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Application of ZnO nanomaterial in inhibiting activity of Erwinia carotovora subsp. carotovora

A technology of carrot soft rot and Erwinia, applied in the fields of application, chemicals for biological control, animal repellent, etc., to achieve the effect of simple equipment, simple operation and low cost

Inactive Publication Date: 2012-12-19
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it has not been reported that ZnO nanomaterials are used as antibacterial materials to inhibit the activity of Erwinia carotovora

Method used

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  • Application of ZnO nanomaterial in inhibiting activity of Erwinia carotovora subsp. carotovora
  • Application of ZnO nanomaterial in inhibiting activity of Erwinia carotovora subsp. carotovora
  • Application of ZnO nanomaterial in inhibiting activity of Erwinia carotovora subsp. carotovora

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Characterization of nano-ZnO structure

[0022] X-ray diffraction (XRD) analysis and transmission electron microscope (TEM) particle size analysis were carried out on the ZnO to be tested. figure 1 It is the XRD analysis result of ZnO nanoparticles, and the diffraction peaks are obviously broadened. According to the Scherrer formula, the ZnO nanoparticle size is 17nm. All the diffraction peaks in the figure can be clearly indexed to the crystal plane diffraction of pure wurtzite crystal ZnO. There are no diffraction peaks of other substances in the XRD pattern, indicating that the nano-ZnO has a high purity.

[0023] From figure 2 It can be seen that nano-zinc oxide is granular, with a particle diameter between 10nm and 20nm, showing a hexagonal crystal form, and the particle size obtained from TEM is in good agreement with the grain size calculated from the half-width of XRD diffraction. The particle size is relatively uniform, and a certain degree of agglomeration...

Embodiment 2

[0029] Add the suspension of Erwinia carotovora soft rot to the nano-ZnO suspension after ultrasonic treatment for 30 minutes to make a bacterial solution containing ZnO concentration of 0.004g / L, and place it in a 28°C constant temperature shaking water bath in the dark for After fully acting for 2 hours at 110rpm, the antibacterial activity of ZnO nanomaterials was detected. From image 3 It can be seen that ZnO nanomaterials have achieved a very good antibacterial effect, and the inhibition rate of Erwinia carotovora can reach 95% at 0.004g / L.

Embodiment 3

[0031] Add the suspension of Erwinia carotovora soft rot to the nano-ZnO suspension after ultrasonic treatment for 30 minutes to make a bacterial solution containing ZnO concentration of 0.005g / L, and place it in a 28°C constant temperature shaking water bath in the dark to avoid light. After fully acting for 2 hours at 110rpm, the antibacterial activity of ZnO nanomaterials was detected. From image 3 It can be seen that ZnO nanomaterials have achieved a very good antibacterial effect, and the inhibition rate of Erwinia carotovora can reach 99% at 0.005g / L.

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Abstract

The invention relates to application of ZnO nanomaterial in inhibiting activity of Erwinia carotovora subsp. Carotovora. The application includes using ZnO independently, or mixing ZnO and streptomycin sulfate and ultrasonically treating for 30 min; adding into a suspension containing Erwinia carotovora subsp. Carotovora; and acting for 2 hr, to inhibit Erwinia carotovora subsp. Carotovora. Compared with prior arts, the application has the advantages of easily accessible materials, low cost, simple operation, simple equipment, and suitability for mass production.

Description

technical field [0001] The invention belongs to the application field of inorganic nanometer materials, and in particular relates to the application of a ZnO nanometer material in inhibiting the activity of Erwinia carotovora. Background technique [0002] Soft rot of Chinese cabbage is a bacterial disease that occurs all over the country. The disease can spread rapidly and expand its damage in the diseased vegetable fields. In severe cases, it can cause more than 50% reduction in Chinese cabbage production. Carotovora subsp. Carotovora (E.carotovora subsp.Carotovora) is the main pathogen of the disease. In addition to damaging Chinese cabbage, this bacteria can also damage potatoes, carrots, onions, peppers, garlic, ginseng, and Clivia. , konjac, tulip, calla lily and other plants, causing different degrees of loss. There are relatively few drugs that can be used to prevent and treat bacterial diseases, and the main traditional drugs for the prevention and treatment of thi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N59/16A01N47/44A01N25/14A01P1/00
Inventor 吴庆生薛敬哲罗志辉
Owner TONGJI UNIV
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