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32results about How to "Short method cycle" patented technology

Method of establishing animal paternity identification system on basis of whole genome STR

The invention relates to the field of genetic resource conservation, particularly relates to the field of animal paternity identification, and discloses a method of establishing an animal paternity identification system on basis of whole genome STR. The method comprises the following steps: 1) looking up whole genome STR loci; 2) preliminarily screening the STR loci; 3) carrying out a primer design; 4) randomly sampling animal populations and extracting genome DNA; 5) verifying the population diversity of the STR loci; 6) carrying out Hardy-Weinberg equilibrium inspection on the STR loci with genetic polymorphism, and screening out the STR loci with the genotype frequency distribution which meets the Hardy-Weinberg equilibrium; 7) evaluating the forensic medicine application value of the STR loci; 8) establishing the STP animal paternity identification system. The method is convenient to operate; the constructed paternity identification system has a paternity exclusion probability of more than 0.99, and is capable of accurately carrying out animal paternity identification, therefore, the paternity identification system can be used for guiding the breeding of rare species, and the identification and breeding of economic species.
Owner:BGI FORENSIC TECH (SHENZHEN) CO LTD

Preparation method of super-hydrophobic-hydrophilic wood with intelligent temperature response

The invention relates to a preparation method of super-hydrophobic-hydrophilic wood with intelligent temperature response and belongs to the field of super-hydrophobic wood. The super-hydrophobic wood prepared by the method has the hydrophilic reversible characteristic which is not owned by the previous super-hydrophilic wood. A contact angle between the super-hydrophobic wood and water is greater than 150 degrees; a rolling angle is smaller than 10 degrees; the super-hydrophobic-hydrophilic wood is excellent in super-hydrophobic performance and good in self-cleaning performance. The preparation method of the super-hydrophobic-hydrophilic wood with the intelligent temperature response comprises the following steps: soaking the wood in a silicon dioxide aqueous solution, drying, then uniformly and dropwise coating the surface of the wood with polycaprolactone dissolved in a mixed solution of methyl alcohol and chloroform, and finally drying in vacuum. The wood shows the intelligent temperature response in the cross section. The method is simple in process, low in cost, short in period, low in energy consumption and free of special complicated equipment; the prepared super-hydrophobic-hydrophilic wood with the intelligent temperature response is capable of converting the wettability for hundreds of times under high and low temperatures; the prepared super-hydrophobic-hydrophilic wood has a great application prospect in the future.
Owner:GUANGDONG YIHUA TIMBER IND

Preparation method of PVDF/silicon dioxide aerogel membrane material

The invention discloses a preparation method of a PVDF / silicon dioxide aerogel membrane material, and belongs to the field of nano thermal insulation materials. According to the method, PVDF and silicon dioxide aerogel are prepared into a spinning precursor solution, a PVDF fiber / silicon dioxide aerogel non-woven membrane material is prepared through two sets of needle heads by adopting the electrostatic spinning technology, then the PVDF / silicon dioxide aerogel membrane material is formed through hot pressing, and the prepared PVDF / silicon dioxide aerogel membrane material is good in flexibility and free of powder falling.
Owner:苏州热象纳米科技有限公司

Tissue culture method of vaccinium dunalianum containing caffeoyl arbutin

The invention discloses a tissue culture method of vaccinium dunalianum containing caffeoyl arbutin, relating to the technical field of tissue culture of plants. A tissue culture seedling culture system is built and the content of caffeoyl arbutin is measured by using improved WPM (woody plant medium) as basal medium at different proportion concentrations of hormone and activated carbon. The growth and root induction of aseptic seedling can be controlled by adjusting the proportion of 6-BA, NAA and the activated carbon. The induction rate of explants reaches 97 percent, the bud seedling is robust, the growth is rapid, and the rooting percentage of the bud seedling is 94%. The method has the advantages of rapid reproduction speed, short period and high content of caffeoyl arbutin, and the like, is an effective way to obtain a large number of caffeoyl arbutin, and provides reliable technical basis for large-scale production of fine rapid propagation seedlings of vaccinium dunalianum.
Owner:SOUTHWEST FORESTRY UNIVERSITY

Zhugen ginger detoxification tissue culture seedling industrialized breeding method

InactiveCN101836586AMeet the needs of large-scale large-scale cultivationHigh induction ratePlant tissue cultureHorticulture methodsVitrificationShoot
The invention discloses a Zhugen ginger detoxification tissue culture seedling industrialized breeding method, which comprises four steps of induction of shoot-tip clustered shoots, subculture multiplication of the clustered shoots, rooting culture and transplantation of test-tube seedlings and respectively optimizes an induction medium, a multiplication medium, a rooting medium, a transplantation medium and the like. The method has high shoot-tip clustered shoot induction rate, high multiple of subculture multiplication of the clustered shoots and low vitrification ratio; the seedling rooting rate reaches 100 percent; the root system is robust and lateral buds sprout; the transplanting survival rate is high and the seedlings are robust and grow quickly; and the method of the invention has short period and low cost, can completely meet the requirements of large-area scale culture of Zhugen ginger and has good application prospect.
Owner:CHONGQING TIANPEI AGRI TECH

Non-dependent production method of gamma-polyglutamic acid from glutamic acid

InactiveCN102586353AAvoid toxicity and growth inhibitory effectsIncrease productionMicroorganism based processesFermentationOrganic acidChemistry
The invention discloses and provides a non-dependent production method of gamma-polyglutamic acid from glutamic acid. The gamma-polyglutamic acid produced by the prior art is low in concentration and high in cost. According to the non-dependent production method disclosed by the invention, an organic acid or a mixture of the organic acid and a nitrogen source is added into a culture medium by flowing during the process of culturing aerobic bacillus sp. to induce thalli to transform a saccharine material and the organic acid into the gamma-polyglutamic acid, and a precursor L-glutamic acid does not need to be additionally added into the culture medium. The fermentation method for producing the gamma-polyglutamic acid, disclosed by the invention, is short in period and easy to operate, can be used for industrial large-scale fermentation and has great application value.
Owner:ZHEJIANG DEQING HUINING BIOTECH

Method of measuring ultrasonic wave transmission time by using chirp signals

The invention discloses a method of measuring ultrasonic wave transmission time by using chirp signals. The method comprises the following steps: an excitation signal is generated by a chirp signal generator to excite a transmitting transducer to transmit ultrasonic wave, after passing through a material to be measured, a transmitted ultrasonic wave signal is received by a receiving transducer, then wavelet filtering processing is performed, afterwards, an ultrasonic wave signal is moved leftwards, the processed ultrasonic wave signal is multiplied by the transmitted ultrasonic wave signal to obtain a product signal, Fourier transform is performed on the product signal to obtain magnitude spectra of the product signal, the frequency of a peak value in the magnitude spectra is determined by using a maximum value function, and finally the transmission time of the ultrasonic wave in the material to be measured is calculated. According to the method, fast Fourier transform (FFT) and signal multiplication are performed only once, the operation burden is greatly lowered, and thus the method is short in implementation period and high in efficiency; by using chirp signals to excite ultrasonic pulse, the ultrasonic penetrability and the time resolution of ultrasonic detection are improved, and thus the detection accuracy rate is improved.
Owner:ZHEJIANG FORESTRY UNIVERSITY

Alcoholic beverage and preparation method of alcoholic beverage

The invention provides an alcoholic beverage and a preparation method of the alcoholic beverage. The alcoholic beverage with low alcohol content, which is high in content of active ingredients, and sour, sweet and chilly in taste, and has the functions of prevention of oxidation, reduction of blood fat, protection of liver and health care, is obtained by taking roselle and gardenia as raw materials, adding edible alcohol and crystal sugar water, adopting a method of supercritical CO2 extraction-molecular distillation, and optimizing a preparation process.
Owner:天津华易科技发展有限公司

Rotating reaction generator and method for repairing organic contaminated soil based on photocatalysis of rotating reaction generator

InactiveCN105798057APromote photochemical reactionsQuick fixContaminated soil reclamationMotor drivePhotocatalytic reaction
The invention discloses a rotating reaction generator and a method for repairing organic contaminated soil based on photocatalysis of the rotating reaction generator. The rotating reaction generator comprises an operation panel, a motor and a rotary stirring cylinder; stirring blades are arranged on the inner wall of the rotary stirring cylinder; and an ultraviolet lamp rod is arranged in a shaft core in the rotary stirring cylinder. The method for repairing the organic contaminated soil by the rotating reaction generator comprises the following steps: after iron-doped mesoporous nanometer titanium dioxide particles are mixed in the organic contaminated soil, the organic contaminated soil is added in the rotary stirring cylinder of the rotating reaction generator; the motor and the ultraviolet lamp rod are started; and the motor drives the rotary stirring cylinder to rotate, so that the organic contaminated soil is fully mixed under the effect of the stirring blades for ultraviolet photocatalysis reaction. The rotating reaction generator can enable the soil to fully contact with ultraviolet rays to accelerate quick and effective photocatalytic degradation of organic matters in the organic contaminated soil, so that the quick and effective repair of the organic contaminated soil is realized.
Owner:HUNAN HENGKAI ENVIRONMENT TECH INVESTMENT CO LTD

Quick seedling cultivation method of Polygonatum sibiricum

The invention relates to a quick seedling cultivation method of Polygonatum sibiricum. The method comprises steps as follows: explant collection; explant treatment; explant cultivation; rooting cultivation; tissue cultured seedling domestication and the like. The novel tissue culture seedling cultivation method is provided through repeated studies and meets the market demand.
Owner:杨文明

Method and device for selecting arcing horn and insulator string in overhead transmission line

The invention provides a method and device for selecting an arcing horn and an insulator string in an overhead transmission line. The selection method comprises the following steps: selecting a presetarcing horn according to an impact withstand voltage value which needs to be borne by the arcing horn in the transmission line, and determining the withstand voltage value of the preset arcing horn;obtaining the withstand voltage value of the insulator string according to the given parameters of insulators and the number of the insulators; calculating effectiveness index parameters of the presetarcing horn for the insulator string according to the withstand voltage value of the preset arcing horn and the withstand voltage value of the insulator string; and determining the arcing horn and the insulator string according to the validity index parameters. On the premise of discharging randomness, selection and matching of the arcing horn and the insulator string are facilitated, reference can be provided for selection and matching of the arcing horn and the insulator string under the condition that a complete test is not needed, the efficiency of selecting the arcing horn and the insulator string is improved, the reliability is high, the period of the method is short, and the cost is low.
Owner:电力规划总院有限公司

Method for recovering byproduct (ammonium chloride) in kresoxim-methyl production process

The invention relates to the technical fields of pesticide and environmental protection, and relates to a byproduct recycling technology in the preparation process of an agricultural bactericide (kresoxim-methyl), in particular to a method for recovering a byproduct (ammonium chloride) in kresoxim-methyl production process. Toluene or petroleum ether is used as an extraction solvent and is mixed with water, a byproduct (crude ammonium chloride) in the kresoxim-methyl production process is used as a raw material, standing and layering are performed after heating, filtrate, which is obtained after the water phase is filtered and subjected to impurity removal, is subjected to cooling crystallization, and finally high-purity ammonium chloride is obtained through centrifugation. The method hasthe advantages of simple process flow, short period, low cost, high product purity (up to 99.6%) and high yield, no solid waste is discharged in industrial production of kresoxim-methyl, and clean production is realized. Meanwhile, the extraction solvent and the ammonium chloride mother liquor can be recycled, and water is saved.
Owner:JINGBO AGROCHEM TECH CO LTD

Method for preparing astragaloside

The invention provides a method for preparing astragaloside, and belongs to the technical field of purification of trace components. The method for preparing the astragaloside includes the following steps that an astragalus membranaceus solution containing alcohol extract of astragalus membranaceus is placed in a high performance liquid chromatography column for elution, and when astragaloside appears, fractions of the astragaloside are collected. Through the method, the high purity astragaloside can be obtained, and the method has a short cycle and simple operation.
Owner:JING BRAND

Preparation method of bovine placenta extract

The invention discloses a preparation method of bovine placenta, and belongs to the technical field of placenta preparation. In the whole preparation process of the bovine placenta, the activity of active ingredients of the bovine placenta is protected by means of temperature control homogenization, enzymolysis and the like, so that the conformation change of the active ingredients in the bovine placenta is effectively avoided, and the bovine placenta has relatively high activity; by adopting the means of centrifugation, filtration and ultrafiltration, impurities are removed, effective components are intercepted, and the produced bovine placenta is small in molecular weight and convenient to absorb by human bodies. The method provided by the invention is short in period, the produced bovine placenta is high in purity and high in activity, the main component is a small molecular peptide fragment, the peptide content of 100-1000Da reaches 95% or above, and 0.05 mg / mL of the bovine placenta can play a role in promoting proliferation of HFF-1 (human skin fibroblasts). The whole production process is pollution-free, free of toxic and side effects, low in cost and good in application prospect.
Owner:WUXI KESHANG BIOLOGICAL TECH

Method for processing meat products by high-temperature fermentation technology

The invention provides a method for processing meat products by using a high-temperature fermentation technology, and belongs to the field of food processing. The method comprises the following stepsof pretreating raw material meat, mixing the pretreated raw material meat with ingredients, rolling and pickling to obtain pickled meat, fermenting the pickled meat by using a fermenting agent, a black bean peel extract and a cocoa extract to obtain fermented meat, and drying, cooking, cooling, packaging and sterilizing the fermented meat to obtain a fermented meat product. The fermenting agent comprises lactobacillus sake, pediococcus acidilactici, staphylococcus vitulinus and staphylococcus carnosus. The method for processing the meat product by utilizing the high-temperature fermentation technology has the advantages of short period and lower cost, and the prepared meat product is safe, good in flavor and high in nutritional value.
Owner:浙江波拉波拉食品股份有限公司 +1

A method for plasma modification of polytetrafluoroethylene microporous membrane

The invention discloses a plasma modification method for a polytetrafluoroethylene microporous membrane. A low temperature plasma treatment method is adopted for conducting surface modification on the PTFE microporous membrane, process parameters of low-temperature plasma treatment, such as the low-temperature plasma gas type, the treatment distance, the radio-frequency power, the treatment time and the gas circulation volume, are controlled, hydrophilia of the PTFE microporous membrane is improved, the PTFE microporous membrane which is originally quite great in hydrophobicity is modified into the PTFE microporous membrane which is quite great in hydrophilia, the water contact angle is lowered to approximately 40 degrees from the original approximately 13 degrees, and meanwhile mechanical strength of the PTFE microporous membrane is maintained. The plasma modification method is short in cycle and low in cost, commercialized production can be conducted, and the application scope of the PTFE microporous membrane is further expanded.
Owner:NANJING UNIV OF SCI & TECH

Feather degradation method

The invention relates to a feather degradation method. The method comprises the following steps: 1, processing the feather with hydrogen peroxide at 30-80DEG C for 6-48h, wherein the mass percent concentration of the hydrogen peroxide is 0.000001-0.01, and the bath ratio of a hydrogen peroxide solution to the feather is 5-30:1; and 2, washing the processed feather with water, and processing with a protease at 30-65DEG C for 6-48h, wherein each gram of the feather is added with 5-200U of the protease, and the bath ratio of a protease solution to the feather is 5-30:1. The method has the advantages of simple operation, thorough feather degradation, large-scale production, no pollution and low energy consumption; and the resultant feather degradation solution can be widely applied.
Owner:DONGHUA UNIV

A method for establishing an animal paternity system based on genome-wide str

The invention relates to the field of genetic resource conservation, particularly relates to the field of animal paternity identification, and discloses a method of establishing an animal paternity identification system on basis of whole genome STR. The method comprises the following steps: 1) looking up whole genome STR loci; 2) preliminarily screening the STR loci; 3) carrying out a primer design; 4) randomly sampling animal populations and extracting genome DNA; 5) verifying the population diversity of the STR loci; 6) carrying out Hardy-Weinberg equilibrium inspection on the STR loci with genetic polymorphism, and screening out the STR loci with the genotype frequency distribution which meets the Hardy-Weinberg equilibrium; 7) evaluating the forensic medicine application value of the STR loci; 8) establishing the STP animal paternity identification system. The method is convenient to operate; the constructed paternity identification system has a paternity exclusion probability of more than 0.99, and is capable of accurately carrying out animal paternity identification, therefore, the paternity identification system can be used for guiding the breeding of rare species, and the identification and breeding of economic species.
Owner:BGI FORENSIC TECH (SHENZHEN) CO LTD

Polypeptide capable of specifically bonding porcine reproductive and respiratory syndrome viruses, screening method thereof and use thereof

The invention relates to a polypeptide capable of specifically bonding porcine reproductive and respiratory syndrome viruses (PRRSV), a screening method thereof and use thereof, and belongs to the field of animal virology. The polypeptide is His Trp Trp Ser Trp Pro Ser Tyr Thr Gln Ser Ser. The invention also provides a screening method of the polypeptide, which comprises: acquiring viruses; screening out phage monoclones with high affinity with the PRRSVs; and sequencing the phage monoclines. The PRRSV inhibition capabilities of the phage monoclines are tested, phage monoclines which can inhibit the duplication of the PRRSVs obviously are selected, and the polypeptide of the selected phage monoclines is the target polypeptide. The polypeptide can be used in PRRSV detection. When marked by an FITC marker, the polypeptide can be used for detecting PRRSVs. The screening method of the polypeptide of the invention is short in period and simple in operation and compared with a monoclonal antibody making method, is quicker and more convenient. The screened polypeptide can be used for detecting the PRRSVs with simple operation and has high specificity and sensitivity.
Owner:国家兽用生物制品工程技术研究中心 +2

Preparation method of intelligent temperature-responsive superhydrophobic-hydrophilic wood

The invention relates to a preparation method of super-hydrophobic-hydrophilic wood with intelligent temperature response and belongs to the field of super-hydrophobic wood. The super-hydrophobic wood prepared by the method has the hydrophilic reversible characteristic which is not owned by the previous super-hydrophilic wood. A contact angle between the super-hydrophobic wood and water is greater than 150 degrees; a rolling angle is smaller than 10 degrees; the super-hydrophobic-hydrophilic wood is excellent in super-hydrophobic performance and good in self-cleaning performance. The preparation method of the super-hydrophobic-hydrophilic wood with the intelligent temperature response comprises the following steps: soaking the wood in a silicon dioxide aqueous solution, drying, then uniformly and dropwise coating the surface of the wood with polycaprolactone dissolved in a mixed solution of methyl alcohol and chloroform, and finally drying in vacuum. The wood shows the intelligent temperature response in the cross section. The method is simple in process, low in cost, short in period, low in energy consumption and free of special complicated equipment; the prepared super-hydrophobic-hydrophilic wood with the intelligent temperature response is capable of converting the wettability for hundreds of times under high and low temperatures; the prepared super-hydrophobic-hydrophilic wood has a great application prospect in the future.
Owner:GUANGDONG YIHUA TIMBER IND

Method of Measuring Ultrasonic Propagation Time Using Chirp Signal

The invention discloses a method for measuring ultrasonic propagation time by using a linear frequency modulation signal, which comprises the following steps: generating an excitation signal through a linear frequency modulation signal generator to excite a transmitting transducer to transmit ultrasonic waves, and the transmitted ultrasonic signal passes through the material to be tested and is received and converted After receiving by the transducer, perform wavelet filter processing, then shift the ultrasonic signal to the left, multiply the processed ultrasonic signal with the transmitted ultrasonic signal to obtain the product signal, perform Fourier transform on the product signal to obtain the amplitude spectrum of the product signal and use the maximum The value function determines the frequency of the peak value in the amplitude spectrum, and finally calculates the propagation time of the ultrasonic wave in the material to be tested; the present invention only needs to perform a Fourier transform FFT and signal multiplication operation, and the operation is greatly reduced, thereby realizing the short cycle and high efficiency of the method; The linear frequency modulation signal is used to excite the ultrasonic pulse, which improves the penetration ability of the ultrasonic wave and the time resolution of the ultrasonic detection, thereby improving the detection accuracy.
Owner:ZHEJIANG FORESTRY UNIVERSITY

Coating method for paint containing plant fragrance

The invention discloses a coating method of a paint containing plant fragrance. The coating method comprises the following steps that S1, the paint containing the plant fragrance is sprayed on the surface of a base material with a spraying thickness of 5[mu] m-10 [mu] m; and S2, the base material sprayed with the paint is heated to 60 DEG C - 120 DEG C, and heat preservation is conducted for 5 min - 10 min. The coating method provided by the invention is easy to coat and easy to adhere to the base material after coating, and the coating method has the advantages of short period and strong adhesive force between the paint and the base material.
Owner:梁景辉

A kind of preparation method of PVDF/silica airgel film material

The invention discloses a method for preparing a PVDF / silicon dioxide airgel film material, which belongs to the field of nano thermal insulation materials. PVDF and silica airgel are prepared into spinning precursor solution, and the PVDF fiber / silica airgel non-woven film is prepared by electrospinning technology through two sets of needles, and then hot-pressed to form The PVDF / silicon dioxide airgel membrane material, the PVDF / silicon dioxide airgel membrane material prepared by the present invention has good flexibility and does not drop powder.
Owner:苏州热象纳米科技有限公司

A Rapid Prototyping Method for Magnetorheological Composite

The invention provides a rapid prototyping method of a magnetorheological composite body, in which a type I magnetorheological elastic material, a type II magnetorheological elastic material, a type I magnetorheological elastic material and a magnetorheological viscoplastic material are used in any The required forms are combined to prepare a magnetorheological composite with non-uniform physical properties. The mixed 3D printing of magnetorheological materials with different physical states and mechanical properties is realized, and flexible digital structure design can be carried out. The forming of solid magnetorheological elastic materials and the packaging of semi-solid magnetorheological viscoplastic materials can be completed at one time. By virtue of the different response characteristics of the mechanical properties of different types of magnetorheological materials under an external magnetic field, it can meet the requirements of complex operating conditions that require magnetorheological materials with different mechanical properties in different positions. The composite prepared by the method of the invention can be used as various functional elements and structural elements such as shock absorbing and damping elements or soft molds for plate forming, and has good performance; meanwhile, the method of the invention has short period, simple process and low cost.
Owner:无锡德昕新材料科技有限公司

A kind of tissue culture method of camphor bilberry

The invention discloses a tissue culture method of vaccinium dunalianum containing caffeoyl arbutin, relating to the technical field of tissue culture of plants. A tissue culture seedling culture system is built and the content of caffeoyl arbutin is measured by using improved WPM (woody plant medium) as basal medium at different proportion concentrations of hormone and activated carbon. The growth and root induction of aseptic seedling can be controlled by adjusting the proportion of 6-BA, NAA and the activated carbon. The induction rate of explants reaches 97 percent, the bud seedling is robust, the growth is rapid, and the rooting percentage of the bud seedling is 94%. The method has the advantages of rapid reproduction speed, short period and high content of caffeoyl arbutin, and the like, is an effective way to obtain a large number of caffeoyl arbutin, and provides reliable technical basis for large-scale production of fine rapid propagation seedlings of vaccinium dunalianum.
Owner:SOUTHWEST FORESTRY UNIVERSITY

A method for screening suitable biochar types in indoor cultivation rice growth test

ActiveCN106022941BApplicability value increasedIncrease the number of solid grainsAgriculture tools and machinesData processing applicationsAgricultural engineeringBiochar
The invention provides a method for screening a proper charcoal type for indoor rice cultivation growth testing. According to the method, processing and statistics are carried out on al rice data in an average rice plant height standard curve, an average rice spike length standard curve, an average effective standard spike and plant number curve, and an average rice filled grain number standard curve at each rice bed and thus an applicability standard curve is drawn. The method can be operated simply. Compared with other methods, the method enables the cost to be reduced by more than 12%. Moreover, the period of the method is short; the efficiency is higher than the efficiency of other methods by more than 19%; the obtain data have small errors; the rice growth situations of all rice beds can be monitored in real time; and the manpower and materials are saved substantially.
Owner:CAPITAL NORMAL UNIVERSITY
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