Method of establishing animal paternity identification system on basis of whole genome STR
A whole-genome, paternity testing technology, applied in the field of paternity testing system establishment, can solve the problems that cannot be ruled out, no public reports, etc., and achieve the effect of low cost, high screening throughput and short cycle
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[0036] Embodiment: the establishment of Crested Ibis STR paternity test system
[0037] Specific steps:
[0038] 1) Define a tandem repeat sequence with a repeat unit of 1 to 6 bp and a total length of not less than 15 bp as STR.
[0039] Tandem Repeat Finder (TRF) was used to search for STRs in the Crested Ibis genome. The search program parameters are set as follows: "Match=2, Mismatch=7, Delta=7, PM=80, PI=10, Minscore=30, Maxperiod=6". A total of at least 820,000 STR loci have been found in the Crested Ibis genome, including about 50,000 STR loci with 4 base repeats.
[0040] 2) Select 300 STR sites from the STR sites with a repeat unit of 4 bp.
[0041] Selection principles: i) the locus is a non-degenerate STR locus; ii) each locus is inherited independently.
[0042] 3) Use the primer design software Primer Premier 5.0 to design upstream and downstream primers in the conserved sequences within 200 bp at both ends of the STR. The design parameters of the primers wer...
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