32 results about "Genotype frequency" patented technology

The present invention provides a method and system for inferring the source of the Han, Tibetan and Uyghur populations of individuals of unknown origin. The method includes: obtaining the genotypes of 94 specific loci of Han, Tibetan, and Uyghur populations by detecting the DNA of individuals of unknown origin; genotype frequency; multiply the genotype frequency of each specific locus to obtain the matching probability values ​​of the 94 specific loci in the Chinese, Tibetan, and Uighur populations; the population with the highest matching probability value is the individual of unknown origin source groups. The scheme of the present invention can realize the inference of the source of the Han, Tibetan and Uighur groups with high accuracy.

The invention relates to the field of genetic resource conservation, particularly relates to the field of animal paternity identification, and discloses a method of establishing an animal paternity identification system on basis of whole genome STR. The method comprises the following steps: 1) looking up whole genome STR loci; 2) preliminarily screening the STR loci; 3) carrying out a primer design; 4) randomly sampling animal populations and extracting genome DNA; 5) verifying the population diversity of the STR loci; 6) carrying out Hardy-Weinberg equilibrium inspection on the STR loci with genetic polymorphism, and screening out the STR loci with the genotype frequency distribution which meets the Hardy-Weinberg equilibrium; 7) evaluating the forensic medicine application value of the STR loci; 8) establishing the STP animal paternity identification system. The method is convenient to operate; the constructed paternity identification system has a paternity exclusion probability of more than 0.99, and is capable of accurately carrying out animal paternity identification, therefore, the paternity identification system can be used for guiding the breeding of rare species, and the identification and breeding of economic species.

The invention discloses a chicken preserved population individual apolegamy optimizing method. Blood DNA of chicken preserved population breed reserving cock and hen individuals is extracted, sequencing is carried out through a reduced genomic sequencing technology, and the sequence is compared with a sequence of the GenBank database reference chicken breed to find SNP loci existing in different individuals. The individual own inbreeding coefficient and the interindividual phylogenetic relationship coefficient are calculated through gene frequency and genotype frequency of the SNP loci, individual apolegamy is optimized according to the statistics value, mating combination of individuals with an overlarge inbreeding coefficient and individuals with an overlarge within-family phylogenetic relationship coefficient is avoided, and the purposes of reducing the preserved population inbreeding level and maintaining the genetic diversity total quantity steady state are achieved. By means of the method, the problem that fine individual apolegamy cannot be carried out depending on genealogy information in the chicken breed resource protection process can be effectively solved, the preserved population inbreeding level can be effectively reduced, the genetic diversity steady state is maintained, and the breed preservation effect and efficiency are improved.

The invention discloses a method for detecting the single nucleoside polymorphism of sheep SPATA6 gene, comprising the following steps: S1 sample selection, S2 cDNA pool construction, S3 PCR amplification, S4 product sequencing and single nucleotide polymorphism analysis Type, S5 sheep SPATA6 gene single nucleotide polymorphism site frequency statistics and its correlation analysis with testis phenotype; Genotype frequency calculation, typing results of fluorescent multiple enzyme ligation reaction technology, testicular phenotype data, and SAS (9.2) software analysis data, and then fully constructed the 72272th T>C mutation of testicular phenotype and spermatogenesis-related gene 6 Relationship of single nucleotide polymorphisms to provide selection markers for breeding high fecundity sheep.