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166 results about "Research model" patented technology

Research Model is a plan for conducting research whereas theory/proposition is an outcome of research. Quantitative research, generally, work out a model based on which the researcher proceeds. The outcome is either approval or rejection of the hypothesis.

Process and system for pricing and processing weighted data in a federated or subscription based data source

InactiveUS20120173381A1Rapid designContinuous captureFinanceCommerceData sourceResearch model
A system and method for collecting handling processing and calculating values weights and prices for observations entered by one or a plurality of sources about one or a plurality of targets related to a researchable model or a theory or practice.
Owner:SMITH STANLEY BENJAMIN

Compounds and Methods for Promoting Angiogenesis

Angiogenesis may be initiated or increased through the use of gamma-secretase inhibitors. The gamma-secretase inhibitor can be a dipeptide class, sulfonamide class, transition state mimic class, benzodiazepine class, or benzocaprolactam class gamma secretase inhibitor. Methods for initiating and increasing angiogenesis are used for disease prevention and treatment as well as for generating research models.
Owner:ANGIOGENETICS SWEDEN AB

Automated research engine

A method of data retrieval and presentation over a network can include, responsive to an inquiry, querying at least one search engine in the network as specified by configuration attributes and the inquiry. References from the query can be received from the at least one search engine. The references can be processed according to research rules and a research model can be generated from the processed references.
Owner:IBM CORP

Apparatus for changing the attack angle of a cavitator on a supercavatating underwater research model

InactiveUS7428870B1Efficiently and quickly and accurately vary angle of attackCheap manufacturingDirection controllersMarine torpedoesResearch modelPower component
An apparatus for changing the angle of attack of a cavitator on a supercavitating underwater research model. The apparatus has a nose assembly that has a pivotable cavitator tilt plate, an actuator member and a drive system engaged with the actuator member to drive the actuator member so as to tilt the cavitator tilt plate to a desired angle. Power components are remotely located and accessible to an operator so as to enable an operator to vary the angle of the cavitator tilt plate while the supercavitating underwater research model is underwater and in motion.
Owner:THE UNITED STATES OF AMERICA AS REPRESENTED BY THE SECRETARY OF THE NAVY

System for storing, displaying, and navigating content data regarding market driven industries

InactiveUS20070276871A1Simplify speed up processEfficient navigationDigital data processing detailsOffice automationMarket drivenResearch model
A model and system employing the model provides an organized structure for storing, displaying, and navigating content data regarding instruments for market driven industries (i.e., securities). A Market Research Model (MRM) paradigm is used to represent elemental concepts, a plurality of specific classes of entities form the MRM, and an interface is used to assemble, maintain, and interact with the model. Information may be added to the model by a research provider and provided to an end user on a subscription basis. The user is provided with an interconnected, navigable model of an item of interest for research and decision-making support.
Owner:PRIMARY GLOBAL RES

System for researching material distribution rule of furnace top equipment

The invention relates to a system for researching a material distribution rule of furnace top equipment and belongs to the technical field of smelting. The system comprises a fully-emulational belt machine loading system, a fully-transparent furnace top material distribution equipment whole model, a fully-transparent furnace body partition research model, an enterprise-level high-speed camera shooting and analysis system and a test platform, wherein the fully-emulational belt machine loading system (1) is arranged at the top end of the whole set of system and is connected with a second layer of platform of the test platform (4) through a moveable bracket; a lower opening of the fully-emulational belt machine loading system (1) is connected with the fully-transparent furnace top material distribution equipment whole model (2); the lower end of the fully-transparent furnace top material distribution equipment whole model (2) is mounted on the fully-transparent furnace body partition research model (3); and a shooting terminal of the enterprise-level high-speed camera shooting and analysis system (5) is generally placed on a first layer of platform of the test platform (4). The invention has the advantages that the research system is comprehensive, complete, intuitive and accurate; and the test scheme is simple and practical, reliable in result and is a new breakthrough in the field of research on a blast furnace material distribution rule.
Owner:BEIJING SHOUGANG INT ENG TECH

High metastatic potential hepatoma cell line capable of steady autophagy indication, and establishment method and application method thereof

InactiveCN102115730AHigh metastatic potentialDynamic observation of autophagy changesMicrobiological testing/measurementMicroorganism based processesMorphological filterIn vivo
The invention belongs to the field of biomedical science, and aims to provide a high metastatic potential hepatoma cell line capable of steady autophagy indication, and an establishment method and an application method thereof. Lentivirus expression vectors containing EGFP (enhanced green fluorescent protein)-LC3 (light chain 3) autophagy reporter genes are constructed and used to infect hepatoma cells with high metastatic potential, so that host cells can express the EGFP-LC3 steadily and efficiently and indicate autophagy changes of the hepatoma cells steadily. To apply the cell line, metastasis research models can be established in vitro and in vivo for the observation on autophagy changes during metastasis, and the cell autophagy changes can be analyzed rapidly and quantitatively by adopting an image analysis method based on Top-hat operators and morphological filter. The high metastatic potential hepatoma cell line has the characteristics that autophagy indication is conducted steadily, reliably and sensitively in a real-time manner, the metastasis occurs definitely, the autophagy is quantified rapidly and accurately, the operation is simple and convenient, and the like, and can greatly facilitate correlation study on autophagy and metastasis.
Owner:ZHONGSHAN HOSPITAL FUDAN UNIV

Steady ultra-hydrophobic surface controllable design method based on geometric analysis

The invention provides a stabilizing ultra-hydrophobic surface controllable design method based on geometric analysis, relating to the fields of material research modeling and simulating material (compute) design, which is characterized in that firstly establishing parallel grating shaped or circular pit and other regular shaped microstructure surface model, according to the established microstructure feature, deriving the apparent contact angle theory predictor formula of the liquid droplet on the microstructure surface based on Wenzel theory and Cassie theory; at the same time, analyzing the C / W converting process on the microstructure surface from the geometric angle; then, pre-designing the geometric parameter of the established microstructure surface model, calculating the apparent contact angle of the liquid droplet on the microstructure surface by the apparent contact angle theory predictor formula derived above, determining whether the designed microstructure surface has ultra-hydrophobicity; at last, calculating h0 of pre-designed microstructure geometric parameter and the corresponding apparent contact angle derived from calculation, judging whether the designed microstructure surface has ultra-hydrophobic stability. The invention is direct viewing, and is easy to operate, which has strong controllability.
Owner:JIANGSU UNIV

Novel lithium battery SOC estimation method based on fractional order model

The invention discloses a novel lithium battery SOC estimation method based on a fractional order model. The method includes: building a simplified fractional order battery model under direct-current excitation on the basis of electrochemical theory; establishing a testing scheme to collect pulse discharging data of a battery under different SOC; recognizing model parameters under different SOC on the basis of a lease square algorithm; building a relation table between the parameters and the SOC after studying change rules of the model parameters and SOC and finding that the model parameters and the battery SOC present obvious monotonic linear tendency; adopting fixed-point theorem to perform iterative calculation for several times on the basis of the relation table between the parameters and the SOC to enable the parameters to gradually converge, and finally acquiring SOC value which is more accurate and stable. The method is a new exploration in estimating battery SOC by building a mathematic function relation between the model parameters and the SOC.
Owner:NANJING FORESTRY UNIV

Combined underground pipe gallery space fire safety research model device

ActiveCN109345938ARealistic reproduction of flow conditionsMeet the requirements of the ventilation modeEducational modelsSource typeEngineering
The invention relates to a combined underground pipeline space fire safety research model device. The device is formed by freely combining a plurality of integrated pipe gallery modules which are of the same structure and detachably assembled together. The combined module includes a variable-frequency ventilation device, a fire source device, a slope adjustment device, a fire detection device anda fire fighting system. One end of main pipe galleries is fixed on a support frame, and the other end is supported by a jack and the slope thereof can be adjusted. A mobile trolley equipped with a burning oil pan and a cable rack can slide in the pipe galleries to change the position of the fire source. The pipe gallery modules can be disassembled and assembled freely. Combined modules can be combined to form a multilayer or multi-compartment pipe gallery. After the fire detection device issues a warning, corresponding fire extinguishing media (such as water or gas) can be provided for fires of different fire source power through the control on the fire fighting system. The device provides an integrated, convenient and flexible small-size comprehensive pipe gallery scale experimental model, which can realize arbitrary combination of different pipe gallery structures, fire fighting systems, fire detection devices, ventilation modes, fire source types and pipe gallery slopes and meet theneeds of experimenters to carry out simulation experiments on the fire conditions of various complex pipe galleries.
Owner:BEIJING UNIV OF TECH

Method for producing heart-specific fluorescence of non-human eukaryotic animals

InactiveUS20050273874A1VectorsSugar derivativesExon intronEukaryotic plasmids
A method of expressing in vivo heart-specific fluorescence in transgenic line of zebrafish is developed, which provides a research model for studying heart-related gene functions and performing gene therapies in the future. The method comprises the following steps. Firstly, a plasmid is constructed. This plasmid construct includes the upstream regulatory region, the exon 1, the intron 1, and the exon 2 of cmlc2 gene, cDNA of GFP, wherein the cmlc2 gene and GFP cDNA form a cassette, and inverted terminal repeats from adeno-associated virus are flanked at both sides of this cassette. The plasmid construct is linearized and microinjected into one-celled zebrafish fertilized eggs. Lastly, the heart-specific fluorescent expressed zebrafish are selected and the germline-transmitting transgenic strain is generated.
Owner:NAT TAIWAN UNIV

Method for zigbee indoor location networking design and corresponding simplification

A method for zigbee indoor location networking design and corresponding simplification belongs to the field of network of things. The method includes determining an area with side length being 6.5-7.5 in actual application to be optimum networking space, selecting common isosceles triangle or equilateral triangle to conduct networking and obtaining a value parameter A by offset estimation. The method simplifies a research process and is favorable for raising a corresponding research model. Effective location data can be obtained under a networking plan by value relation only related to a parameter n obtained by reduction by means of the proportional relation of three points and a coordinate value. The method enables a location process to be free of interference of the parameter A and the parameter n and only needs to obtain relative function relation with the parameter n after parameter elimination and known reference point coordinate under the corresponding relation. Therefore, corresponding coordinate is calculated, and the location aim can be achieved. By means of the method, networking density and the networking plan are scientifically designed. The method provides an analysis base for proceeding of a scientific experiment and application cost.
Owner:BEIJING UNIV OF TECH

Construction method for mouse model with conditionally deleted Tmem30a genes and with pancreatic beta cells and application

The invention discloses a construction method for a mouse model with conditionally deleted Tmem30a genes and with pancreatic beta cells and an application. The construction method comprises the following steps: constructing a conditionally deleted Tmem30a gene homozygote mouse, wherein the two ends of one or more exon of the Tmem30a genes are inserted into homonymous arrayed loxP locus; mating the mouse with pancreatic beta cell specific transgenic mouse Ins2-Cre, thereby acquiring the mouse model with conditionally deleted Tmem30a genes and with pancreatic beta cells. The mouse with conditionally deleted Tmem30a genes and with pancreatic beta cells shows glucose intolerance and poor insulin sensitivity. The mouse model can be used as a diabetes research model.
Owner:成都基康医药科技有限公司

Method for producing heart-specific fluorescence of non-human eukaryotic animals

A method of expressing in vivo heart-specific fluorescence in transgenic line of zebrafish is developed, which provides a research model for studying heart-related gene functions and performing gene therapies in the future. The method comprises the following steps. Firstly, a plasmid is constructed. This plasmid construct includes the upstream regulatory region, the exon 1, the intron 1, and the exon 2 of cmlc2 gene, cDNA of GFP, wherein the cmlc2 gene and GFP cDNA form a cassette, and inverted terminal repeats from adeno-associated virus are flanked at both sides of this cassette. The plasmid construct is linearized and microinjected into one-celled zebrafish fertilized eggs. Lastly, the heart-specific fluorescent expressed zebrafish are selected and the germline-transmitting transgenic strain is generated.
Owner:NAT TAIWAN UNIV

Moon driving apparatus for earth-moon driving instrument

The invention relates to a moon drive device of an earth-moon transmission instrument, comprising a moon body and an orbit ring, wherein the inner bottom surface of the orbit ring is provided with an annular fixed orbit, the inner side surface of the orbit ring is provided with a moon body autorotation gear ring, the orbit ring is movably mounted with an annular movable ring inside, the movable ring is mounted on the annular fixed orbit by a movable ring riding wheel, the moon body is mounted on the movable ring by a connection base and a rotation base; the outside of the movable ring is mounted with a moon body revolution gear ring which is meshed with a transmission gear driven by a power transmission shaft arranged at the outside of the orbit ring; the moon body is internally provided with an autorotation transmission shaft and a gear variable transmission mechanism, the movable ring is mounted with a primary transmission gear which is meshed with the moon body autorotation gear ring on the orbit ring, and the primary transmission gear drives the moon body to auto-rotate on the movable ring. The invention has reasonable structure, and the autorotation and revolution of the moon body are simple and independent. The invention is suitable for being mounted on the earth-moon transmission instrument as the moon drive device, and can be used as a teaching and scientific research model and as a large sight-seeing apparatus in public entertainment locations, and integrates the function with the sight effect.
Owner:孙成夫

Method for establishing SD mouse fat and diabetes research model through diet and STZ induction

The invention provides a method for establishing an SD mouse fat and diabetes research model through diet and STZ induction. Through high-fat feed and STZ induction, the attacking characteristics of fat and diabetes of humans are induced, and an animal model for establishing pathogenesis of fat and type-2-diabetes caused by insulin resistance and partial defects of pancreatic beta cells is provided. Mice are divided into a normal control group, a fat group and a diabetes group, and each group has six SD male mice. The high-fat feed is adopted for feeding mice for eight weeks, a pure-fat insulin resistance model is induced, after the mice are fed with the high-fat feed for eight weeks, injection of STZ small doze in batch is conducted, and establishment of the diabetes animal model is induced. The method can better simulate the attacking characteristics of human type-2-diabetes caused by improper diet and enjoinment factors currently, and compared with other modeling, the method has better scientific significance of clinical and fundamental research combination.
Owner:任慧雯

Method for steel production contract plan and multi-level inventory matching optimization under MTO-MTS management mode

The invention belongs to the field of management science and engineering and particularly relates to a steel production contract plan compilation method based on multi-level inventory matching under an MTO-MTS mixed production mode. A logical frame of the method is composed of five modules which are a contract pool establishing module, a multi-level inventory matching module, a multi-process production plan module, an inventory and production collaboration module and a human-computer interaction and evaluation module, wherein the inventory and production collaboration module is a system core, a multi-level inventory and production plan joint optimization algorithm based on a rematch collaboration strategy is adopted for the inventory and production collaboration module, and the contract plan compilation problem under a mixed mode is solved by being combined with parameters coming from the human-computer interaction and evaluation module and coordinating the multi-level inventory matching module and the multi-process production plan module according to the output of the contract pool establishing module. The method is based on an operational research model and an intelligent algorithm, is the contract plan compilation method based on multi-level inventory matching and has important theoretical significance and wide application values for improving flexibility of a steel enterprise production management system.
Owner:SHANGHAI UNIVERSITY OF FINANCE AND ECONOMICS

Construction method for plant root related functional gene research model

The invention provides a construction method for a plant root related functional gene research model. The method comprises the following steps of: connecting a plant root related functional gene to a transgenic expression vector pRI101-AN to obtain recombinant plasmid containing a target gene and transferring the recombinant plasmid into agrobacterium rhizogene K599; infecting a part which is 0.2-0.5cm below a cotyledon of a living cucumber seedling germinated for 4 days, and inducing to form a transgenic hairy adventitious root; shearing off a main root below the part where the hairy root isformed when the hairy adventitious root grows to be 5-10cm long; burying the formed hairy adventitious root into soil; and continuously growing for 1-2 months to obtain the plant root related functional gene research model. In the method, by using a cucumber plant with the transgenic hairy adventitious root, a cucumber root related functional candidate gene can be quickly identified, and the identification can be performed without obtaining the transgenic plant, so that an experimental procedure can be greatly reduced, the defect of low transgenic regeneration efficiency is overcome, and quick analysis of functions of the root related gene can be performed on a large scale.
Owner:HANGZHOU NORMAL UNIVERSITY

Simulation model and method of DC circuit breaker

The invention relates to a simulation model and method of a DC circuit breaker. The simulation model comprises a main branch, a transfer branch and an energy consumption branch which are connected inparallel, wherein the main branch comprises a switch part, a first controlled voltage source and a first bidirectional conduction circuit, the switch part, the first controlled voltage source and thefirst bidirectional conduction circuit are connected in series, the transfer branch comprises a second controlled voltage source and a second bidirectional conduction circuit, the second controlled voltage source and the second bidirectional conduction circuit are connected in series, and the energy consumption branch comprises a resistor and a controlled power supply which are arranged in parallel. An existing model for working performance research model of the DC circuit breaker is simplified, and the performance simulation efficiency of the DC circuit breaker is improved.
Owner:GLOBAL ENERGY INTERCONNECTION RES INST CO LTD +2

Water-landing load test device and method for water surface aircraft model under fixed posture

The invention discloses a water-landing load test device and method for a water surface aircraft under a fixed posture. The water-landing load test device and method for the water surface aircraft under the fixed posture aims at the accurately predicted difficulty that the water-landing load of a current water surface aircraft, and is used for the fine evaluation of the water-landing load in the fixed state of the water surface aircraft. The method can ensure that a certain parameter of the model remains unchanged in the water-landing process, the influence of a model attitude angle, the horizontal velocity, the vertical velocity and wave parameters on the water-landing load is studied, and the influence of the aerodynamic lift on the water-landing load on the water surface is studied by means of unloading. During testing, measurement data of an acceleration sensor is used for accurately analyzing the water-landing load, and a distribution region and a distribution rule of the pressurein the bottom of the model are analyzed by using measurement data of a pressure sensor, and a load factor and pressure distribution produced by the model in any of the poses which may occur and sea conditions which may occur are accurately predicted.
Owner:CHINA SPECIAL TYPE FLIER RES INST

Power transmission line icing real-time drawing method for plateau micro-terrain

The invention discloses a power transmission line icing real-time drawing method for a plateau micro-terrain. The method comprises the steps of step 1, constructing a K.J model and calculating a real-time ice thickness value of each spatial site by utilizing real-time meteorological parameters such as heavy rainfall, air liquid water content, a horizontal wind speed of a vertical lead wire and an air temperature value; step 2, calculating standard ice thickness of each spatial site; step 3, performing spatial interpolation for a Kriging model to obtain a Kriging interpolation ice zone distribution diagram A; step 4, according to the conditions of historical ice observation data, terrain factors and meteorological factors, performing zone division of an ice chart in a multi-year return period; step 5, establishing altitude and ice thickness distribution models of different similar icing zones; step 6, obtaining an ice zone distribution diagram B; and step 7, making a research on a real-time ice zone distribution model. Therefore, the problems of high cost, low efficiency, high risk and the like for manual ice observation caused by the defect of non full consideration of a power transmission line icing research model in real-time meteorology and plateau micro-terrain in the prior art are solved.
Owner:ELECTRIC POWER RESEARCH INSTITUTE, CHINA SOUTHERN POWER GRID CO LTD +1

Boost converter model-based negative regulation voltage suppression condition analysis method

The invention relates to a Boost converter model-based negative regulation voltage suppression condition analysis method. The method comprises the following steps of 1: building a Boost converter research model; 2: determining key parameters involved by negative regulation voltage leap by analyzing a mechanism of generating a negative regulation voltage in a CCM-CISM mode; 3: building a negative regulation voltage mathematic model, and analyzing influence of inductance and capacitance on the negative regulation voltage; 4: determining inductance and capacitance selection conditions of suppressing the negative regulation voltage; and 5: performing simulation and test verification. For the problems of an instable system, negative regulation of an output voltage, reduced system response speed and the like caused by right half plane zero in a Boost converter mathematic model, a converter parameter design principle of suppressing the negative regulation voltage is provided, an analysis method for solving the problem is given, and a used modeling form is of important significance for a reference provided for analyzing the problem.
Owner:XIAN UNIV OF SCI & TECH +1

Assistant experiment teaching aid for space cognition and light environment simulation

InactiveCN101436360AImplement static imagesRealize functionEducational modelsCognitionEngineering
The invention relates to an assistant experimental teaching aid for spatial cognition and luminous environment simulation, which comprises an experimental box main body, a sunlight simulator, an image acquirer, a sliding worktable and a shielding fabric cover, wherein the sliding worktable bears a research model, the sunlight simulator simulates sunlight projection scenes at different moments, the image acquirer selects proper shooting pots for shooting, and the shielding fabric cover is sleeved on the experimental box main body and used for shielding external light. The teaching aid can accurately simulate the running path track of the sun, acquire static or dynamic building model images under simulation environment, and effectively aids teaching research of the spatial cognition and the luminous environment simulation in building design.
Owner:HUAZHONG UNIV OF SCI & TECH

System for inducing nerve stem cell differentiation and inducing method thereof

The invention discloses a method for preparing neural stem cells, which comprises a step of inductively culturing P19 cells in N2B27 culture medium to obtain a cell group containing neural stem cells above 94%. The method can culture the P19 cells in an environment without blood serum and retinoic acid, thus suppressing the interference of the blood serum with complex components and preventing the obtained neural stem cells from being transformed posteriorly by retinoic acid. Moreover, the method can directly transform pluripotent stem cells to neutral stem cells without resulting in selective cell apoptosis. The neutral stem cells obtained by the invention have anterior neutral plate characteristics and totipotency, and can well simulate the neurogenesis process in body. Accordingly, the neural stem cells can be used as the research model for analyzing neural induction and neural differentiation process from epiblast to neuroderm, thus providing an ideal path for researching development of embryo after nidation.
Owner:SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI

Filtering method and device for network malicious information

The invention discloses a filtering method and device for network malicious information and relates to the technical field of computer information processing and information filtering. The filtering method disclosed by the embodiment of the invention comprises the following steps of: obtaining text information to be filtered, system pre-research model information and user feedback model information; pre-treating the text information to be filtered; carrying out characteristic information matching on the pre-treated text information to be filtered and the system pre-research model information to obtain a first matched result; carrying out the characteristic information matching on the pre-treated text information to be filtered and user feedback model information to obtain a second matched result; and carrying out filtering treatment on the text information to be filtered according to the first matched result and the second matched result. With the adoption of the filtering method and device of the network malicious information, disclosed by the embodiment of the invention, the automatic filtering performance for the malicious information is improved and system information can be automatically updated.
Owner:NEW FOUNDER HLDG DEV LLC +2

Waste machine tool key part residual life prediction and evaluation method

The invention relates to a method for predicting and evaluating the residual life of key parts of a waste machine tool and belongs to the technical field of green remanufacturing systems. According tothe method for predicting and evaluating the residual life of the key part of the waste machine tool, a main failure form of the key part of the machine tool is considered to be fatigue fracture failure, and a continuous fatigue damage model based on fracture mechanics is adopted to predict the life of the waste part so that remanufacturability is determined. The research model better conforms tothe actual working condition so that the prediction and evaluation result is more accurate, precision of predicting and evaluating the main shaft key part model is ensured, residual life calculationaccuracy of the model is ensured, and precision of predicting and evaluating spindle key part models is ensured, meanwhile, data of the secondary load loading fatigue test are compared with the calculation result of the model, and accuracy, effectiveness and feasibility of the corrected model are verified; practice proves that prediction precision of the residual life prediction and evaluation method can meet the actual engineering requirement of waste part remanufacturability evaluation.
Owner:SHENYANG POLYTECHNIC UNIV

Subsynchronous oscillation assessment method of electric power system

ActiveCN105281324AAccurate Simulation ForecastEffectively analyze the degree of impactForecastingAc network circuit arrangementsElectric power systemPower grid
The invention discloses a subsynchronous oscillation assessment method of a power system including a Unified Plow Flow Controller (UPFC) and belongs to the technical field of power system planning and operating. The method firstly establish a subsynchronous oscillation equivalent research model of the UPFC, then makes proper equivalent simplification of the power system according to a network structure and a generator and obtains an equivalent model from the generator to the UPFC, and finally uses the equivalent model from the generator to the UPFC for assessment and prediction of subsynchronous oscillation behaviors of the power system. The method achieves subsynchronous oscillation assessment of the power system with the UPFC and can be used to guide planning and safe and stable operation of a power grid.
Owner:STATE GRID JIANGSU ECONOMIC RES INST +1

Mouse prostate cancer circulating tumor cell line and prostate cancer circulating tumor cell isolating and culturing method

The invention discloses a mouse prostate cancer circulating tumor cell line and a prostate cancer circulating tumor cell isolating and culturing method. The preservation number of the circulating tumor cell line is CCTCC C201601. The isolating and culturing method comprises the following steps: establishing an in-situ prostate cancer animal model, taking blood, lysing red blood cells, and conducting isolating and culturing and passage. Obtained circulating tumor cells are uniform in quality; based upon immunofluorescence as well as scratch and membrane-penetrating experiments, the obtained circulating tumor cells conform to the properties of the circulating tumor cells and the circulating tumor cells are relatively strong in transfer and invasion capacities; and the circulating tumor cells can serve as a good experimental tool for researching a prostate cancer transfer mechanism in a laboratory and can also provide a cell research model for preventing prostate cancer transfer in the clinical field. The circulating tumor cell isolating and culturing method provided by the invention is simple and easy to operate, and the isolated cells can undergo stable passage, so as to facilitate such experiments as researching the properties of the circulating tumor cells and the like in the laboratory. The circulating tumor cell line and the circulating tumor cell isolating and culturing method provided by the invention can have a broad application scope in the field of researching tumor transfer.
Owner:FOURTH MILITARY MEDICAL UNIVERSITY
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