The invention relates to a preparation method of targeting tumor vasculature Anxa1 marked precursor NOTA-IF7, which belongs to the fields of organic chemistry and medicines. According to the invention, a double-function chelating agent 1,4,7-triazacyclononane-1,4,7- triaceticacid (1,4,7-triazacyclononane-1,4,7- triaceticacid, p-SCN-Bn-NOTA, NOTA for short) is coupled with specifically combined tumor vasculature 7 peptide amino acid (isoleucine-phenylalanine-leucine-leucine-tryptophan-glutamine-arginine, IF7), and a HPLC one-step method is used for purifying to obtain NOTA-IF7. THE preparation method of NOTA-IF7 is simple, the reaction condition of the technology is mild, the yield is high, the product can be used for isotope mark of fluorine-18, copper-64 and gallium-68, and is conveniently prepared to a positron photographic developer for tumor.