65 results about "Disease susceptibility" patented technology

The present invention relates to methods of assessing disease susceptibility associated with dietary and lifestyle risk factors. The invention provides for analysis of alleles at loci of genes associated with lifestyle risk factors, and the disease susceptibility profile of an individual is determined by reference to datasets which further match the risk factor with lifestyle recommendations in order to produce a personalized lifestyle advice plan.

The present invention relates to allelic variants of human Spinocerebellar ataxia 2 (SCA2) gene and provides allele-specific primers and probes suitable for detecting these allelic variants for applications such as molecular diagnosis, prediction of an individual's disease susceptibility, and / or the genetic analysis of SCA2 gene in a population.

The invention generally relates to the field of diagnostic or prognostic assays and in particular relates to assays for the detection of antibodies in a sample comprising patient bodily fluid, wherein such antibodies are used as biological markers of a disease state or disease susceptibility. The assay is based on cross-titration of both the patient bodily fluid to be tested for the antibody and an antigen used to detect the antibody by specific binding.

The invention relates to a method of detecting a disease state or disease susceptibility in a mammalian subject which comprises detecting an antibody in a test sample comprising a bodily fluid from said mammalian subject wherein said antibody is a biological marker of a disease state or disease susceptibility, the method comprising: (a) contacting said test sample with a plurality of different amounts of an antigen specific for said antibody, (b) detecting the amount of specific binding between said antibody and said antigen, (c) plotting or calculating a curve of the amount of said specific binding versus the amount of antigen for each amount of antigen used in step (a) and (d) determining the presence or absence of said disease state or disease susceptibility based upon the amount of specific binding between said antibody and said antigen at each different antigen concentration used.

Computer controlled methods, systems and apparatus for detecting an infectious agent in a host cell, an animal cell, or plant cell, a body fluid or tissue sample to provide information useful to diagnose a disease or prognosticate disease susceptibility, extent or outcome are provided. In one illustrative embodiment, the infected animal cell is a Chlamydia infected mononuclear phagocyte in a blood sample. In another illustrative embodiment, the infected body fluid is a blood sample infected with “fee floating”Chlamydia. Detection and preferably, quantification of such an infected mononuclear phagocyte(s) or blood sample provides information useful in diagnosing or prognosticating susceptibility, extent or outcome of patients suspected of suffering from vascular, coronary or central nervous disease(s) or disorder(s).

The invention relates to a method of detecting a disease state or disease susceptibility in a mammalian subject which comprises detecting an antibody in a test sample comprising a bodily fluid from said mammalian subject wherein said antibody is a biological marker of a disease state or disease susceptibility, the method comprising: (a) contacting said test sample with a plurality of different amounts of an antigen specific for said antibody, (b) detecting the amount of specific binding between said antibody and said antigen, (c) plotting or calculating a curve of the amount of said specific binding versus the amount of antigen for each amount of antigen used in step (a) and (d) determining the presence or absence of said disease state or disease susceptibility based upon the amount of specific binding between said antibody and said antigen at each different antigen concentration used.

The invention relates to a composite growth regulator for beet stress-resistant yield and sugar-increased seedling stage and a using method thereof. The composite growth regulator is prepared from plant hormone, Ca2 and various signal substances, is particularly suitable for saline and arid environment conditions, and can induce the expression of beet stress-resistant genes and accelerate coordination and unification between the beet and the environment, the individual and population, the root and the overground part, the external form of the plant and internal physiological function. After the beet is transplanted, the salinization resistance, drought resistance and disease resistance can be effectively enhanced; and the problem that the beet yield and the sugar content resulted from the influence of salinization, drought and disease spreading are reduced year by year is solved. The regulator also has the characteristics of a common strong-seedling agent and also can solve the problems of vain growth, disease susceptibility and weak seedlings of the young seedlings in the beet seeding culture process; the beet root yield under the saline and arid environment conditions reaches 47,267.47kg / hm<2>, the yield is increased by 16 to 18 percent; the sugar content is 16.1 percent, and is increased by 0.9 to 1.2 percent; and the effects of increasing the yield and sugar are obvious.

A privacy-preserving method for performing a disease susceptibility test on a patient, said method comprising:(I) performing homomorphic computations,(J) obtaining a test result which is partly decrypted with a first part (prk1 resp. prk2) of a private key,(L) decrypting said partly decrypted result with a second part (prk2 resp. prk1) of said private key,wherein said homomorphic computations are based on encrypted genomic markers of the patient, on encrypted clinical and / or environmental markers, and on encrypted ancestry markers of the patient.The invention is also related to a method for inferring ancestry in the encrypted domain.

The present invention relates to methods of assessing disease susceptibility associated with dietary and lifestyle risk factors. The invention provides for analysis of alleles at loci of genes associated with lifestyle risk factors, and the disease susceptibility profile of an individual is determined by reference to datasets which further match the risk factor with lifestyle recommendations in order to produce a personalized lifestyle advice plan.

The invention discloses a method for identifying resistance to a bacterial wilt disease of mulberry. The method comprises the steps of: inoculating damaged roots into bacterial wilt pathogenic bacteria liquid after planting different resistant varieties or strains of mulberry seedlings with certain seedling age to be identified, a disease-resistance control mulberry seedling and a disease-susceptibility control mulberry seedling indoors by water, controlling epidemic factors, then investigating disease incidence and evaluating the resistance according to a resistance evaluation standard to obtain mulberry varieties and strains with different disease resistances. The method has the advantages of simplicity in operation, accurate and reliable result and good repeatability, and has great application potential in the aspects such as identifying of resistance to bacterial wilt disease of the mulberry, evaluating of a disease-resistant resource, screening of a disease-resistant breeding material and the like.

The invention generally relates to the field of diagnostic or prognostic assays and in particular relates to assays for the detection of antibodies in a sample comprising patient bodily fluid, wherein such antibodies are used as biological markers of a disease state or disease susceptibility. The assay is based on cross-titration of both the patient bodily fluid to be tested for the antibody and an antigen used to detect the antibody by specific binding.

The invention discloses applications of a corn molecular marker in identification and regulation of maize rough dwarf disease resistance trait. According to the present invention, the corn molecular marker is a DNA molecule obtained by amplifying with a primer pair A1 by using the genomic DNA of corn as a template, wherein the DNA fragment containing the sites 7418-9965 of a sequence 5 can be obtained through amplifying with the A1 by using the genomic DNA of corn as the template; and the experiment results prove that the corn molecular marker is significantly correlated with the maize rough dwarf disease resistance trait, and can be used for identifying the maize rough dwarf disease resistance trait, wherein the ZmGDI2 gene with no the sites 7418-9965 of the sequence 5 can significantly increase the disease susceptibility of corn to rough dwarf disease, and the ZmGDI1 gene containing the sites 7418-9965 of the sequence 5 can significantly increase the disease resistance of corn to rough dwarf disease, such that the disease resistance of corn to rough dwarf disease can be regulated by using the ZmGDI2 gene and the ZmGDI1 gene.

The invention relates to a method of detecting a disease state or disease susceptibility in a mammalian subject which comprises detecting an antibody in a test sample comprising a bodily fluid from said mammalian subject wherein said antibody is a biological marker of a disease state or disease susceptibility, the method comprising: (a) contacting said test sample with a plurality of different amounts of an antigen specific for said antibody, (b) detecting the amount of specific binding between said antibody and said antigen, (c) plotting or calculating a curve of the amount of said specific binding versus the amount of antigen for each amount of antigen used in step (a) and (d) determining the presence or absence of said disease state or disease susceptibility based upon the amount of specific binding between said antibody and said antigen at each different antigen concentration used.

A privacy-preserving method for performing a disease susceptibility test on a patient, said method comprising:(I) performing homomorphic computations,(J) obtaining a test result which is partly decrypted with a first part (prk1 resp. prk2) of a private key,(L) decrypting said partly decrypted result with a second part (prk2 resp. prk1) of said private key,wherein said homomorphic computations are based on encrypted genomic markers of the patient, on encrypted clinical and / or environmental markers, and on encrypted ancestry markers of the patient.The invention is also related to a method for inferring ancestry in the encrypted domain.

The present invention relates to business methods for discovery of therapeutic and diagnostic products by identifying variations that occur in the human genome, relating these variations to one another, and, ultimately, relating these variations to the genetic bases of phenotype such as disease resistance, disease susceptibility or drug response.

Disclosed herein our novel methods of increasing resistance of Citrus plants to diseases, in particular, citrus canker disease caused by Xanthomonas citri ssp. citri and Huanglongbing disease. Some embodiments relate to novel methods of altering gene sequence, structure and expression of certain disease susceptibility genes in the Citrus plant. Other embodiments relate to gene constructs equipped to be introduced into Citrus cells and direct modifications to target gene sequences.

The invention relates to the field of the association analysis between the gene-environmental interaction and the disease susceptibility, in particular to a method for judging the disease susceptibility of severe depression through detecting the associative action between a NET gene and negative life events. The method solves the problem that the disease susceptibility of the severe depression cannot be accurately judged yet in prior art through detecting the NET gene only or evaluating the negative life events only. First, the study object that is peripheral blood leukocyte DNA is extracted by a conventional phenol-chloroform method; the polymerase chain reaction and the direct sequencing DNA technique are adopted to detect the polymorphism of SNPs rs2242446 and rs5569 which are the NET gene; the genotype and the allele of examinees are determined; then the screening and the judgement of susceptible population are evaluated and performed through combining a life event scale so as to find that individuals are easier to be attacked by tristimania when suffering from the stimulus of certain life events, wherein, NET gene rs2242446 polymorphism basic groups of the individuals are C, and rs5569 polymorphism basic groups thereof are A.

The invention discloses a method for predicating gene age and disease susceptibility and a kit, and belongs to the technical field of medical biology and gene diagnosis. The method comprises the following steps: extracting cellular genome DNA of a host; testing polymorphic sites of genes (RTEL1 gene, ACYP2 gene, TERC gene, TERT gene, NAF1 gene, OBFC1 gene and ZNF208 gene) of a subject; and predicating the gene age and the disease susceptibility of the subject. According to the method, the correlation between the polymorphic sites of seven genes and senescence gene is described for the first time; the method can be applied to predication, auxiliary diagnosis and treatment of diseases caused by the senescence gene, and can be also applied to research and development of new drugs in order to guide the subject to start a healthy life.

Atopic asthma is a chronic, inflammatory lung disease characterized by recurrent breathing problems in response to an allergen. Platelets play an important role in this allergic inflammatory process, by releasing preformed mediators like platelet factor 4 (PF4) and regulated upon activation in normal T cells expressed and secreted (RANTES) upon activation causing eosinophil chemotaxis. The present invention relates to allelic variants of the human Inositol polyphosphate 4-phosphatase (INPP4A) gene and splice variants of the coding sequence, which encodes INPP4A enzyme known to be an important regulator of platelet activation; and provides primers and methods suitable for the detection of these allelic variants for applications such as molecular diagnosis, prediction and prevention of an individual's disease susceptibility, and / or the genetic analysis of the INPP4A gene in a population. The invention also provides an association with the expression profile of INPP4A protein in the mouse model of asthma. Specifically, the invention provides a method for detection of predisposition to atopic disorders / other immunological disorders such as, autoimmune disorders, inflammatory disorders, cancer, multiple sclerosis, fibrosis, tuberculosis, sarcoidosis, hypertension and disorders developing due to hypertension, diabetes and disorders developing due to diabetes, alcohol abuse, anxiety, asthma, chronic obstructive pulmonary disease (COPD), cholecystectomy, degenerative joint disease (DJD), seizure disorder, arthritis, etc. where human Inositol polyphosphate 4-phosphatase (INPP4A) might play an important role due to its involvement in platelet action.

The invention relates to a kit for detecting disease susceptibility, in particular to a kit for detecting breast cancer susceptibility. The kit comprises specific primers used for detecting No. rs2294021 SNP locus on Foxp3 gene and No. rs2569190 SNP locus on CD14 gene, and a specific endonuclease used for detecting the two SNP loci. The estimation on the breast cancer susceptibility is realized by detecting polymorphism of the No. rs2294021 SNP locus on the Foxp3 gene and the No. rs2569190 SNP locus on the CD14 gene.

The invention discloses a method for improving the survival rate of forsythia cuttings, including 1) seedling selection, 2) soil quality selection, 3) fertilizer supplementation and sterilization, and 4) temperature control. In terms of water control, regular spraying and moisturizing are required to ensure cutting branches No wilting, after the seedlings are cut, maintain high temperature and humidity, and timely and proper disinfection measures prevent the cutting seedlings from being attacked by diseases and insect pests, ensure the good development of the seedling root system, rapid reproduction, and avoid the occurrence of less roots, root loss, aging, and disease susceptibility, etc. defect.

The invention provides an early screening method and a kit for non-alcoholic fatty liver disease susceptibility genes. In particular, the difference of non-alcoholic fatty liver disease gene spectrums of Chinese people and European and American people is considered, and a combination of SNP loci of genes related to non-alcoholic fatty liver disease susceptibility of Chinese people is screened out; wide (high-throughput detection sites and high-throughput detection samples) screening and inspection are carried out on genetic markers related to the non-alcoholic fatty liver disease by using a nucleic acid mass spectrometer. The method disclosed by the invention is high in detection success rate, good in technical reproducibility and high in cost performance, can realize multi-gene detection of a single small sample, and meets maximum use of the small sample; The method has the technical advantages of high accuracy and high sensitivity, the detection result is stable, and the detection positive rate is increased.

The invention discloses a functional specificity molecule marker Pi7FNP of blast-resistance gene Pi7, a method and an application thereof. The molecule marker consists of Pi7-1FNP and Pi7-2FNP, wherein Pi7-1FNP is the nucleotide sequence amplified from rice total DNA by SEQ ID NO.1 and SEQ ID NO.2, and Pi7-2FNP is the nucleotide sequence amplified from rice total DNA by SEQ ID NO.3 and SEQ ID NO.4. According to the invention, two single-base differences which can be different from the Pi7 functional specificity of the disease-susceptibility alleles and other blast-resistance genes are gained by the analysis through comparing allele sequences of the rice blast-resistance gene Pi7 with the sequences of SEQ ID NO.5 and SEQ ID NO.6; primers SEQ ID NO.1 and primers SEQ ID NO.2 and primers SEQ ID NO.3 and primers SEQ ID NO.4 are respectively obtained by designing; by carrying out DNA amplification and then specific enzyme digestion on rice, the Pi7-1FNP and Pi7-2FNP are respectively obtained. The invention can be applied in screening from large amounts of rice genetic resources and identifying the functional resistant genes, and molecular marker assisted selection, gene polymerization breeding and transgenic breeding.

The invention discloses a polymorphism marker site, primer pair and detection kit used for identifying a disease resistance / disease susceptibility character of peach trees to meloidogyne incognita andapplication. The polymorphism marker site is a site with a sequence, on the positions 4, 467, 346-4, 467 and 380 bp with 35 bp deletion, of the second chromosome of a peach genome. The PCR primers designed according to upstream and downstream 200 bp sequences of the site can be used for identifying or assisting in identifying the disease resistance / disease susceptibility character of the peach trees to the meloidogyne incognita and has high accuracy when used for identifying the disease resistance / disease susceptibility character of the peach trees to the meloidogyne incognita. Verification is conducted on the PCR marking accuracy of 250 to-be-detected peach single plants of an F2 group cross-bred by prunus kansuensis and prunus persica, and a result shows that when phenotypic character prediction of a hybrid group is conducted, the accuracy can reach 100.00%; therefore, the PCR primers developed by means of the marker site have the advantages of being simple, fast and low in cost when used for detection, and large-scale application in production can be achieved.

Specific PON1 and ACHE alleles segregate in linkage, forming an haplotype which directly correlates with higher susceptibility to develop Parkinson's Disease (PD). This PD-susceptibility haplotype is herein presented as a tool for predicting the risk of developing Parkinson's Disease and its severity, both for an individual and for the population in general. Thus, the present invention provides the use of said PD-susceptibility haplotype in diagnostic and screening methods.

The invention discloses a susceptibility gene of hyperthyroidism disease and an application thereof. Specifically, the invention discloses 20 single nucleotide polymorphisms (SNP) related to human hyperthyroidism disease susceptibility. The invention, for the first time, also confirms that the 20 single nucleotide polymorphisms are closely related to hyperthyroidism disease. These SNPs can be used to detect hyperthyroidism susceptibility, prepare a reagent and a kit for detecting susceptibility of hyperthyroidism disease and screen hyperthyroidism medicines and the like, thereby providing a new way for diagnosis, typing, prognosis and treatment of hyperthyroidism disease.

Applicants have successfully generated heritable phenotypes in plants making them resistant to bacterial blight. TAL effector binding elements, (EBEs) of bacterial pathogen disease susceptibility genes are modified to prevent induction of expression associated with disease states caused by the bacterial pathogens. Surprisingly, Applicants have found that modifications may be made in the EBEs of these genes which prevent bacterial pathogen induction, but still allow for normal plant development and seed production. Nucleic acid sequences for generating such plants, amino acid sequences, cells, vectors and expression constructs are included as well as resistant plants, seeds and lines.

The invention discloses disease susceptibility associated SNP sites, especially relates to coronary heart disease susceptibility substantially-associated SNP sites of a susceptible region chr5p15, and applications of the SNP sites in the assessment of the susceptibility of the coronary heart disease, and belongs to the biotechnological field. The SNP sites are a series of SNP sites positioned in a segment of a closely linked region of a TRIO gene region of a chromosome 5p15, comprising rs117297784, rs1058312, rs9942367, rs17500919 and rs11133787. According to a method for determining the coronary heart disease susceptible populations through the variation characteristics of the haplotype composed of the SNP sites in the TRIO gene region of the chromosome 5p15 and a series of the SNP sites, the populations without early-stage clinical symptoms of the coronary heart disease, especially coronary heart disease high-risk populations with traditional danger factors, can be non-invasively, rapidly and simply screened, so coronary heart disease susceptible objects can be early discovered, and corresponding health measures can be adopted, thereby the incidences of the coronary heart disease and myocardial infarction are reduced