Applications of corn molecular marker in identification and regulation of maize rough dwarf disease resistance trait

Abstract

The invention discloses applications of a corn molecular marker in identification and regulation of maize rough dwarf disease resistance trait. According to the present invention, the corn molecular marker is a DNA molecule obtained by amplifying with a primer pair A1 by using the genomic DNA of corn as a template, wherein the DNA fragment containing the sites 7418-9965 of a sequence 5 can be obtained through amplifying with the A1 by using the genomic DNA of corn as the template; and the experiment results prove that the corn molecular marker is significantly correlated with the maize rough dwarf disease resistance trait, and can be used for identifying the maize rough dwarf disease resistance trait, wherein the ZmGDI2 gene with no the sites 7418-9965 of the sequence 5 can significantly increase the disease susceptibility of corn to rough dwarf disease, and the ZmGDI1 gene containing the sites 7418-9965 of the sequence 5 can significantly increase the disease resistance of corn to rough dwarf disease, such that the disease resistance of corn to rough dwarf disease can be regulated by using the ZmGDI2 gene and the ZmGDI1 gene.

Description

technical field [0001] The invention relates to the application of maize molecular markers in identifying and regulating maize rough dwarf resistance traits in the field of biotechnology. Background technique [0002] Maize (Zea Mays L.) is the largest food crop in my country and plays an important role in China's energy and food security. Maize rough dwarf disease is a worldwide viral disease, which seriously endangers the production of maize. Since it was discovered in my country's Xinjiang and Gansu regions in 1954, maize rough dwarf disease has broken out in a large area in various production areas of my country, especially in the Huanghuaihai region, causing large-scale destruction or extinction of production, posing a serious threat to corn production. In 2007, nearly 53,300 hm in Shandong Province 2 Maize production was extinct due to rough dwarf disease. In 2008, Shandong Province suffered from corn rough dwarf disease, which was rare in history. The occurrence are...

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Problems solved by technology

[0006] At present, the prevention and control of rough dwarf disease mainly depends on two methods: chemical control and adjustment of sowing date: chemical control pollutes the environment and increases costs; adjustment of sowing date has great limitations, which not only wastes light and temperature resources, but also easily suffers from later natural disasters. disaster, reduced production

Therefore, the selection of maize inbred lines with high resistance to rough dwarf disease is the basis of disease-resistant breeding, but most of the varieties currently marketed are at the level of high or moderate susceptibility, and there are few varieties with better resistance. The source is relatively simple, mainly from the American hybrid P78599

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