Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for detecting single nucleotide polymorphism of SPATA6 gene of sheep and application

A technology of single nucleotide polymorphism and polymorphism, which is applied in the fields of genomics, biochemical equipment and methods, and microbial measurement/inspection, etc., can solve problems such as scarcity, and achieve convenient PCR amplification and DNA double strands Unchained effect

Active Publication Date: 2019-09-17
甘肃润牧生物工程有限责任公司
View PDF5 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the lack of research on the relationship between SPATA6 gene and sheep testis size, the research on the function of this gene and the relationship between the genetic variation of this gene and the development of testis are blank

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting single nucleotide polymorphism of SPATA6 gene of sheep and application
  • Method for detecting single nucleotide polymorphism of SPATA6 gene of sheep and application
  • Method for detecting single nucleotide polymorphism of SPATA6 gene of sheep and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0063] The technical solution of the present invention will be further described in detail below in conjunction with the accompanying drawings, but the protection scope of the present invention is not limited to the following description.

[0064] A method for detecting single nucleoside polymorphism of sheep SPATA6 gene, comprising the following steps:

[0065] S1 sample selection: Collect the testes and epididymis of 299 6-month-old Hu sheep with the same feeding conditions after slaughter, and measure the weight and volume of the testes and epididymis;

[0066] Construction of S2 cDNA pool:

[0067] a) Utilize chloroform to extract RNA in testicular tissue, the specific steps are as follows: 1) randomly select 100 samples from 229 samples, take about 0.2g respectively, and then quickly put them into a sterilized mortar pre-cooled with liquid nitrogen In the mortar, pour liquid nitrogen into the mortar and quickly grind it into a fine powder; add 1mL TransZol to a sterilize...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for detecting single nucleotide polymorphism of a SPATA6 gene of sheep. The method comprises the following steps of S1, selecting samples; S2, constructing a cDNA pond; S3, performing PCR amplification; S4, performing product sequencing and single nucleotide polymorphism subtyping; and S5, performing frequency statistics of single nucleotide polymorphism sites of the SPATA6 gene of the sheep and analyzing the correlation between the frequency statistics and testis phenotypes. The invention further provides an application of the detected nucleotide polymorphism of the SPATA6 gene of the sheep. Through genotypic frequency calculation, the subtyping result of a fluorescence multiple enzyme connection reaction technique, testis phenotype data and SAS(9.2) software analysis data, the relationship between the testis phenotype and the single nucleotide polymorphism mutated by the 72272nd T>C of the spermatogenesis related gene 6 is sufficiently constructed, and high-fecundity sheep are bred for screening and labeling.

Description

technical field [0001] The invention relates to the field of screening and detection of sheep single nucleoside polymorphism (SNP) molecular markers, in particular to a method and application for detecting the single nucleoside polymorphism of sheep SPATA6 gene. Background technique [0002] Single nucleotide polymorphism (single nucleotide polymorphism, SNP), as the most common form of genetic variation, refers to the polymorphism of DNA sequence caused by the conversion, transversion, insertion and deletion of a single base at the genome level. attitude. This molecular marker has a large number and wide distribution. According to whether it is in the protein coding region, it can be divided into coding SNP and non-coding SNP. There are relatively few cSNPs in the coding region, but they are of great significance in the study of genetic diseases. Therefore, The study of cSNPs in the coding region has attracted more attention. The cSNP in the coding region of the gene can ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6888C12Q1/6806G16B20/20
CPCC12Q1/6888C12Q1/6806G16B20/20C12Q2600/156C12Q2600/124C12Q2531/113C12Q2521/107
Inventor 乐祥鹏王丽秦芳李发弟李万宏
Owner 甘肃润牧生物工程有限责任公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com