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Multiple testing methods for gene mapping

A technology of gene positioning and testing methods, applied in the field of bioinformatics, to achieve the effect of improving precision and accuracy, improving utilization rate, and shortening cycle

Active Publication Date: 2017-07-25
HANGZHOU HEYI GENE TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Previously traditional markers (such as SSR, RFLP, etc.) could not realize the possibility of performing two analyzes simultaneously in one independent experiment

Method used

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  • Multiple testing methods for gene mapping

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Embodiment Construction

[0027] Embodiments of the present invention are described in further detail below in conjunction with accompanying drawings:

[0028] The invention discloses a multiple testing method for gene positioning, which is characterized in that it comprises:

[0029] Step 1: Sequence the parental DNA samples and offspring DNA samples using second-generation sequencing technology to obtain high-precision short-segment sequences;

[0030] Step 2: using SNP analysis software to compare or cluster the obtained high-precision short fragment sequences with reference sequences to obtain accurate SNP information for each sample;

[0031] Step 3: convert such SNP information into population SNP, further convert into standard molecular standard format, use mapping software to construct genetic map, and perform QTL analysis on the basis of genetic map to obtain QTL locus information;

[0032] Step 4: Identify the phenotypic information of the progeny DNA samples, and accurately count the distri...

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Abstract

The invention discloses a multiple detection method for gene positioning, which is characterized in that it comprises: step 1: sequence the DNA samples of parents and offspring to obtain high-precision short fragment sequences; step 2: sequence the obtained high-precision DNA samples The short fragment sequence is compared with the reference sequence or clustered with each other to obtain accurate SNP information; Step 3: convert the SNP information into a population SNP, and further convert it into a standard molecular standard format to construct a genetic map, and on the basis of the genetic map Step 4: Identify the phenotypic information of the progeny DNA samples, and accurately count the distribution of phenotypic values, group the phenotypic values ​​according to gradients, and group the extreme phenotypic values ​​corresponding to the offspring The high-precision short fragment sequences of the first-generation DNA samples are mixed separately, the genotype frequency distribution is analyzed, and the relevant significant regions are found through the chi-square test and the rank sum test; Step 5: Integrate the QTL locus information and Step 4 to obtain accurate gene positioning information.

Description

technical field [0001] The invention relates to the technical field of biological information, in particular to a multiple testing method for gene positioning. Background technique [0002] The simplified genome method is a technical method that uses enzyme digestion technology, sequence capture chip technology or other experimental methods to reduce the complexity of species genomes, and then study various genetic structural variations of genomes. Currently common simplified genome technologies include RAD (Restriction site Associated DNA) and GBS (Genotyping By Sequencing). These technologies can develop tens of thousands of SNP markers in a very short period of time, and molecular markers are the basis for genetic mapping, association analysis, population genetic analysis, and ecological diversity analysis. Scientific research is a popular application of current second-generation sequencing technology. [0003] BSA (Bulked Segregation Analysis or Mixed Segregation Analy...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G06F19/22C12Q1/68
Inventor 刘三阳范崇仪钱晓菊张新明高金龙罗亚丹范玉美赵艳艳王兆宝
Owner HANGZHOU HEYI GENE TECH
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