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A method for establishing an animal paternity system based on genome-wide str

A whole-genome, paternity testing technology, applied in the field of paternity testing system establishment, can solve the problems that cannot be ruled out, no public reports, etc., and achieve the effect of low cost, high screening throughput and short cycle

Active Publication Date: 2017-01-18
BGI FORENSIC TECH (SHENZHEN) CO LTD
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  • Summary
  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

[0007] 2. In the case that certain marker genes of the offspring are from the biological father, and it is assumed that the father also carries these genes, it cannot be ruled out that it is the biological father of the offspring
[0010] Through literature search, do not see the public report identical with the present invention

Method used

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  • A method for establishing an animal paternity system based on genome-wide str
  • A method for establishing an animal paternity system based on genome-wide str
  • A method for establishing an animal paternity system based on genome-wide str

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0036] Example: Establishment of Crested Ibis STR Paternity Identification System

[0037] Specific steps:

[0038] 1) A tandem repeat sequence with a repeating unit of 1-6 bp and a total length of not less than 15 bp is defined as STR.

[0039] Tandem Repeat Finder (TRF) was used to search for STRs in the crested ibis genome. The search procedure parameters are set as follows: "Match=2, Mismatch=7, Delta=7, PM=80, PI=10, Minscore=30, Maxperiod=6". A total of at least 820,000 STR sites were found in the crested ibis genome, of which about 50,000 STR sites with 4-base repeats were found.

[0040] 2) 300 STR sites were selected from STR sites with a repeating unit of 4 bp.

[0041] Selection principle: i) This locus is a non-degenerate STR locus; ii) Each locus is inherited independently.

[0042] 3) Use the primer design software Primer Premier 5.0 to design upstream and downstream primers respectively in the conserved sequences within 200bp of both ends of the STR. The pa...

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Abstract

The invention relates to the field of genetic resource conservation, particularly relates to the field of animal paternity identification, and discloses a method of establishing an animal paternity identification system on basis of whole genome STR. The method comprises the following steps: 1) looking up whole genome STR loci; 2) preliminarily screening the STR loci; 3) carrying out a primer design; 4) randomly sampling animal populations and extracting genome DNA; 5) verifying the population diversity of the STR loci; 6) carrying out Hardy-Weinberg equilibrium inspection on the STR loci with genetic polymorphism, and screening out the STR loci with the genotype frequency distribution which meets the Hardy-Weinberg equilibrium; 7) evaluating the forensic medicine application value of the STR loci; 8) establishing the STP animal paternity identification system. The method is convenient to operate; the constructed paternity identification system has a paternity exclusion probability of more than 0.99, and is capable of accurately carrying out animal paternity identification, therefore, the paternity identification system can be used for guiding the breeding of rare species, and the identification and breeding of economic species.

Description

technical field [0001] The invention relates to a method for establishing a paternity identification system, in particular to a method for establishing a paternity identification system for animals based on STR sites in a whole genome sequence. Background technique [0002] Whether it is the protection of rare animal germplasm resources or the breeding of animals with high economic value, there are more and more events requiring individual identification and paternity testing of animals. For example, the paternity test and individual identification of giant pandas can help to avoid the problem of inbreeding in artificial breeding, and it is of great significance to save the giant panda species. For another example, in the modern horse breeding and racing industry, the identification of the origin of the horses of some excellent breeds is also highly valued. [0003] Because DNA molecular markers can directly reflect the changes in the genetic material itself or the genome, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q2600/156
Inventor 李生斌李波刘清波常辽熊子军
Owner BGI FORENSIC TECH (SHENZHEN) CO LTD
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