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A method and application for detecting single nucleotide polymorphism of sheep spata6 gene

A single nucleotide polymorphism, sheep technology, applied in genomics, biochemical equipment and methods, microbial determination/inspection, etc., can solve problems such as scarcity

Active Publication Date: 2022-05-17
甘肃润牧生物工程有限责任公司
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Problems solved by technology

Due to the lack of research on the relationship between SPATA6 gene and sheep testis size, the research on the function of this gene and the relationship between the genetic variation of this gene and the development of testis are blank

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  • A method and application for detecting single nucleotide polymorphism of sheep spata6 gene
  • A method and application for detecting single nucleotide polymorphism of sheep spata6 gene
  • A method and application for detecting single nucleotide polymorphism of sheep spata6 gene

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Embodiment Construction

[0063] The technical solution of the present invention will be further described in detail below in conjunction with the accompanying drawings, but the protection scope of the present invention is not limited to the following description.

[0064] A method for detecting single nucleoside polymorphism of sheep SPATA6 gene, comprising the following steps:

[0065] S1 sample selection: Collect the testes and epididymis of 299 6-month-old Hu sheep with the same feeding conditions after slaughter, and measure the weight and volume of the testes and epididymis;

[0066] Construction of S2 cDNA pool:

[0067] a) Utilize chloroform to extract RNA in testicular tissue, the specific steps are as follows: 1) randomly select 100 samples from 229 samples, take about 0.2g respectively, and then quickly put them into a sterilized mortar pre-cooled with liquid nitrogen In the mortar, pour liquid nitrogen into the mortar and quickly grind it into a fine powder; add 1mL TransZol to a sterilize...

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Abstract

The invention discloses a method for detecting the single nucleoside polymorphism of sheep SPATA6 gene, comprising the following steps: S1 sample selection, S2 cDNA pool construction, S3 PCR amplification, S4 product sequencing and single nucleotide polymorphism analysis Type, S5 sheep SPATA6 gene single nucleotide polymorphism site frequency statistics and its correlation analysis with testis phenotype; Genotype frequency calculation, typing results of fluorescent multiple enzyme ligation reaction technology, testicular phenotype data, and SAS (9.2) software analysis data, and then fully constructed the 72272th T>C mutation of testicular phenotype and spermatogenesis-related gene 6 Relationship of single nucleotide polymorphisms to provide selection markers for breeding high fecundity sheep.

Description

technical field [0001] The invention relates to the field of screening and detection of sheep single nucleotide polymorphism (SNP) molecular markers, in particular to a method and application for detecting the single nucleotide polymorphism of sheep SPATA6 gene. Background technique [0002] Single nucleotide polymorphism (single nucleotide polymorphism, SNP), as the most common form of genetic variation, refers to changes in DNA sequences at the genome level due to conversions, transversions, insertions, and deletions of a single base. polymorphism. This molecular marker has a large number and wide distribution. According to whether it is in the protein coding region, it can be divided into coding SNP and non-coding SNP. There are relatively few cSNPs in the coding region, but they are of great significance in the study of genetic diseases. Therefore, The study of cSNPs in the coding region has attracted more attention. The cSNP in the coding region of the gene can be div...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12Q1/6806G16B20/20
CPCC12Q1/6888C12Q1/6806G16B20/20C12Q2600/156C12Q2600/124C12Q2531/113C12Q2521/107
Inventor 乐祥鹏王丽秦芳李发弟李万宏
Owner 甘肃润牧生物工程有限责任公司
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