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Runzhou crested white duck variety identification method

A variety identification, Runzhou Phoenix technology, applied in the fields of molecular biology and animal husbandry, can solve the problem of not being able to identify which species it is

Pending Publication Date: 2019-08-16
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Morphological identification refers to the appearance characteristics of animal breeds, but there are many animal breeds that cannot be identified based on their appearance characteristics, pure or hybrid.

Method used

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  • Runzhou crested white duck variety identification method
  • Runzhou crested white duck variety identification method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0011] A kind of identification method of crested white duck in Runzhou, the specific steps are as follows:

[0012] 1. Materials and methods

[0013] 1.1 Experimental materials

[0014] Runzhou Crested White Duck, Peking Duck, Cherry Valley Duck, Liancheng White Duck, Ji'an Red-feathered Duck, Yutian Shelduck, Jinding Duck, Chaohu Duck, Sansui Duck, Shaoxing Duck, Putian Black Duck, Jingxi Hemp Duck , Linwu duck, Jingjiang duck, Dayu duck, Sumu duck, green-winged duck, Youxian duck, Sichuan duck, Zhongshan duck, Jianshui yellow-brown duck, mountain duck, Muscovy duck, Guangxi duck Shelduck, Gaoyou Duck, Mawang Duck.

[0015] 1.2 Extraction of duck genomic DNA

[0016] 30 individuals of each duck breed were collected, and blood was collected from the duck wing vein. The total genomic DNA was extracted by the traditional phenol-chloroform method, and the DNA concentration and purity were measured. The DNA was diluted to 100ng / L and stored in a -20°C refrigerator.

[0017] 1...

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PUM

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Abstract

The invention relates to a Runzhou crested white duck variety identification method, and belongs to the technical field of zootechny and molecular biology. By the aid of microsatellite loci provided by pertinent literature, microsatellite DNA (deoxyribonucleic acid) seats with common genotypes are screened to perform STR (short tandem repeat) type sequencing, data are analyzed by a Microsatellite-Toolkit, genotype frequency is counted, and Runzhou crested white ducks are identified by the aid of common genotype bands of all varieties.

Description

technical field [0001] The invention relates to a method for identifying the breed of Runzhou crested duck, which belongs to the technical fields of animal husbandry and molecular biology. Background technique [0002] In 2005, Yangzhou University and the Zhenjiang Waterfowl Research Institute discovered crested ducks in a group of ducks in a natural lake in the Runzhou area of ​​​​Zhenjiang. After nearly 10 years of work, in 2013, they successfully recovered the black-billed ducks that are consistent with ancient literature records. Crested duck—Runzhou crested white duck. [0003] Traditional species identification methods are based on morphological, biochemical and molecular markers of animal groups. Morphological identification refers to the appearance characteristics of animal breeds, but there are many animal breeds that cannot be identified based on their appearance, pure or hybrid. Molecular markers are identified based on mutations in proteins and nucleic acid mol...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 常国斌王统苗刘本帅张莘陈国宏张扬王志秀白皓江勇
Owner YANGZHOU UNIV
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