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Rapid positioning method for industrial hemp trait related genes

A technology of industrial hemp and positioning method, which is applied in the fields of genomics, plant genetic improvement, botanical equipment and methods, etc., can solve the problems of late start and unlocated related functional genes, and achieve the effect of rapid positioning

Pending Publication Date: 2021-03-30
DAQING BRANCH OF HEILONGJIANG ACAD OF SCI
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AI Technical Summary

Problems solved by technology

[0003] The invention provides a method for rapidly locating genes related to industrial hemp traits, which solves the problems that the molecular biology of industrial hemp starts late and the relevant functional genes have not yet been located.

Method used

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  • Rapid positioning method for industrial hemp trait related genes
  • Rapid positioning method for industrial hemp trait related genes
  • Rapid positioning method for industrial hemp trait related genes

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Experimental program
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Effect test

Embodiment 1

[0027] (1) Variety selection: Jindao-15 with high fiber content was selected as the male parent, and Huoma No. 1 with low fiber content was selected as the female parent for hybridization.

[0028] (2) Population construction: Jindao-15 and Huoma No. 1 have the same flowering period, and the two varieties are planted at the same time. The male plants of Huoma No. 1 are pulled out at the budding stage, and the obtained hybrid is the F1 generation. Select industrial hemp plants of the same gender for listing in the early stage, take young leaves and freeze them in liquid nitrogen, store them in a -80°C refrigerator for later use, harvest industrial hemp at the mature stage of the process, and measure the original stem weight and fiber weight of a single plant after fresh peeling , to calculate the fiber content. The calculation formula is as follows: fiber content=fiber weight / original stem weight×100%.

[0029] (3) SLAF library construction and high-throughput sequencing: Acco...

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Abstract

The invention discloses a rapid positioning method for industrial hemp trait related genes, and belongs to the technical field of industrial hemp research. The method comprises the following steps: variety selection, extreme group construction, specific site amplified fragment library construction and high-throughput sequencing, marker development, correlation analysis, gene annotation and real-time fluorescent quantitative PCR. The method comprises the following steps: constructing a high-low mixing pool for F1-generation groups obtained by hybridizing parents with relatively large target character differences, re-sequencing the parents, sequencing the high-low mixing pool by adopting a simplified genome deep sequencing technology, developing an SLAF tag, carrying out single nucleotide polymorphism detection, and carrying out marker correlation analysis on genotype frequency differences among the mixing pools. According to the method, a target character related candidate region is obtained, most plant populations at least need to be propagated to F2 generations to perform gene localization, and due to the high heterozygosity characteristic of industrial hemp, gene localization isrealized through F1 generations, so that rapid localization of industrial hemp genes can be realized in a high-efficiency and low-cost manner.

Description

technical field [0001] The invention belongs to the technical field of industrial hemp research, and in particular relates to a method for rapidly locating genes related to industrial hemp traits. Background technique [0002] Industrial hemp (Cannabis Sativa.L) is an annual herb plant of the genus Cannabis in the family Cannabinaceae. Its tetrahydrocannabinol (THC) content is less than 0.3%. The stems, leaves and seeds of industrial hemp are all Economic utilization value, widely used in industries such as textile, construction, papermaking, medicine and food, etc. The main goal of industrial hemp breeding can be formulated according to the needs of specific industries. At present, industrial hemp breeding mainly adopts traditional breeding methods, but this method cultivates New varieties not only have a long cycle but also are difficult to achieve customized breeding goals. The molecular breeding method can not only improve the breeding efficiency but also achieve the pur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/6858G16B20/20G16B25/20G16B30/10A01H1/02
CPCC12Q1/6895C12Q1/6858G16B20/20G16B25/20G16B30/10A01H1/02C12Q2600/13C12Q2600/156C12Q2531/113C12Q2535/122C12Q2537/165C12Q2563/107C12Q2545/113
Inventor 赵越王晓楠曹焜张晓艳边境孙宇峰张治国朱浩王盼韩承伟姜颖徐磊
Owner DAQING BRANCH OF HEILONGJIANG ACAD OF SCI
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