Chicken preserved population individual apolegamy optimizing method

Abstract

The invention discloses a chicken preserved population individual apolegamy optimizing method. Blood DNA of chicken preserved population breed reserving cock and hen individuals is extracted, sequencing is carried out through a reduced genomic sequencing technology, and the sequence is compared with a sequence of the GenBank database reference chicken breed to find SNP loci existing in different individuals. The individual own inbreeding coefficient and the interindividual phylogenetic relationship coefficient are calculated through gene frequency and genotype frequency of the SNP loci, individual apolegamy is optimized according to the statistics value, mating combination of individuals with an overlarge inbreeding coefficient and individuals with an overlarge within-family phylogenetic relationship coefficient is avoided, and the purposes of reducing the preserved population inbreeding level and maintaining the genetic diversity total quantity steady state are achieved. By means of the method, the problem that fine individual apolegamy cannot be carried out depending on genealogy information in the chicken breed resource protection process can be effectively solved, the preserved population inbreeding level can be effectively reduced, the genetic diversity steady state is maintained, and the breed preservation effect and efficiency are improved.

Description

technical field [0001] The invention discloses a method for selecting and optimizing individuals of chicken conservation populations, and belongs to the fields of conservation genetics and molecular biology. Background technique [0002] Scientifically protecting the genetic diversity carried by my country's local chicken breed resources is of great significance to the sustainable development of my country's and the world's poultry industry. [0003] At present, local chicken breed resources are mainly protected by small-scale family conservation populations at all levels of conservation farms and gene banks, and living preservation is the most important protection method. For limited-scale conservation populations, inbreeding and genetic drift will lead to loss of genetic diversity during the continuation of generations. The development trend of resource conservation in the future is to maintain the stable state of genetic diversity of a breed to the greatest extent with t...

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is aimed at the problem that in the process of chicken species conservation, relying on pedigree information cannot carry out fine management of individual matching (especially for endangered resource groups), which will lead to the problem that the total amount of genetic diversity of the preserved population will be reduced to varying degrees, and provides A method for individual selection and optimization of chicken conservation population

Images