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Tissue culture method of vaccinium dunalianum containing caffeoyl arbutin

A technique for caffeoyl arbutin and tissue culture, which is applied in the field of plant tissue culture, can solve problems such as lack of resources of camphor bilberry, and achieves the effects of good application prospect, low price and simple culture process.

Active Publication Date: 2014-08-27
SOUTHWEST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Prior art At present, there is no report on obtaining high-content caffeoylarbutin plant materials by tissue culture technology, and it is necessary to use modern biotechnology to solve the problem of resource scarcity in Vaccinium camphoria

Method used

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  • Tissue culture method of vaccinium dunalianum containing caffeoyl arbutin
  • Tissue culture method of vaccinium dunalianum containing caffeoyl arbutin

Examples

Experimental program
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Effect test

Embodiment 1

[0030] (1) Initiation culture of axillary bud induction

[0031] Obtain the young and tender vaccinia vaccinia stems of the year as explants from robust vaccinia plants, cut off the leaves of the explants and cut them into 4-6 cm long stems, soak them in detergent water for 30-60 minutes , rinse with running water for 1 to 2 hours, then disinfect with 75% alcohol and 0.1% mercuric chloride, soak in 75% alcohol for 15 to 30 seconds, soak in 0.1% mercuric chloride for 7 to 12 minutes, and rinse with sterile water for 4 to 10 minutes after disinfection. 5 times, cut it into a stem section with 1-2 leaf axils on sterilized filter paper and blot the water on the stem section, then insert the leaf axil upwards obliquely in the axillary bud induction medium (WPM+6-BA2.5mg / L+NAA1.0mg / L+activated carbon 2.5g / L, in which white sugar 20~30g / L, agar 4-6g / L, pH5.5), the culture temperature is 25±2℃, the light intensity is 2000lx, light The cycle ratio is 14h:10h. After 25 days of cultur...

Embodiment 2

[0039] (1) Initiation culture of axillary bud induction

[0040] Obtain the young and tender vaccinia vaccinia stems of the year as explants from robust vaccinia plants, cut off the leaves of the explants and cut them into 4-6 cm long stems, soak them in detergent water for 30-60 minutes , rinse with running water for 1 to 2 hours, then disinfect with 75% alcohol and 0.1% mercuric chloride, soak in 75% alcohol for 15 to 30 seconds, soak in 0.1% mercuric chloride for 7 to 12 minutes, and rinse with sterile water for 4 to 10 minutes after disinfection. 5 times, cut it into a stem section with 1-2 leaf axils on sterilized filter paper and blot the water on the stem section, then insert the leaf axil upwards obliquely in the axillary bud induction medium (WPM+6-BA2mg / L +NAA1.5mg / L+activated carbon 3g / L, in which white sugar 20~30g / L, agar 4~6g / L, pH5.4), culture on 27±2℃, light intensity 2500lx, light cycle ratio 15h :9h. After 30 days of cultivation, the bud induction rate was ...

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Abstract

The invention discloses a tissue culture method of vaccinium dunalianum containing caffeoyl arbutin, relating to the technical field of tissue culture of plants. A tissue culture seedling culture system is built and the content of caffeoyl arbutin is measured by using improved WPM (woody plant medium) as basal medium at different proportion concentrations of hormone and activated carbon. The growth and root induction of aseptic seedling can be controlled by adjusting the proportion of 6-BA, NAA and the activated carbon. The induction rate of explants reaches 97 percent, the bud seedling is robust, the growth is rapid, and the rooting percentage of the bud seedling is 94%. The method has the advantages of rapid reproduction speed, short period and high content of caffeoyl arbutin, and the like, is an effective way to obtain a large number of caffeoyl arbutin, and provides reliable technical basis for large-scale production of fine rapid propagation seedlings of vaccinium dunalianum.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for tissue culture of vaccinia camphora rich in caffeoylarbutin. Background technique [0002] Vaccinium dunalianum is a plant belonging to the genus Vaccinium of Ericaceae, an evergreen shrub. It is mainly produced in Yunnan, Sichuan, Guizhou, Tibet and other places. It is also distributed in Taiwan, Sikkim, Bhutan, India (northeast), Myanmar (northeast) to Vietnam. In Yunnan Province, it is mainly distributed in the northwestern part of Yunnan Province. From the Central Yunnan Plateau to southern Yunnan and southeastern Yunnan. Its young leaf buds are shaped like a sparrow's beak and are conical, so it is also called "sparrow's mouth tea" among the Yi people in Yunnan. According to records, it has been used as a tea substitute for a long time since the Ming Dynasty. effect. Plants of the genus Vaccinium are rich in various nutrients and have...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 赵平唐军荣罗旭璐阚欢刘云李旭
Owner SOUTHWEST FORESTRY UNIVERSITY
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