140results about How to "High degradation activity" patented technology

The invention relates to the technical field of sewage treatment, and in particular to preparation method of magnetic biochar loaded photosynthetic bacteria material and a sewage treatment method. The preparation method comprises the steps of: preparing a magnetic nano iron oxide material by a chemical coprecipitation method, conducting activation and expansion culture on photosynthetic bacteria, loading the nano iron oxide particles on the surface of biological carbon material through a self-assembly method to form a magnetic biochar; and loading the photosynthetic bacteria on the surface of the magnetic biochar through a co-incubation method, so as to obtain the magnetic biochar loaded photosynthetic bacteria material. The magnetic biochar loaded photosynthetic bacteria material is co-incubated with sewage for a period of time, so as to realize degradation of COD, ammonia nitrogen and phosphate radical in the sewage. The invention employs the electrostatic binding of high specific surface of the nano magnetic iron oxide and the bacteria with charge on the surface to achieve immobilization of strains, and magnetic iron oxide material has activation on strain enzyme, thereby enhancing the degradation of COD, ammonia nitrogen and phosphate radical by microbes.

The invention discloses a denitrogenation paracoccus strain and the cultivating method and the application. The strain is Paracoccus denitrificans W12 CGMCC No.1673. It has the strong degradation ability to organic pollutant, and could be used to take the biology treatment to the polluted water containing benzene, xylene, quinoline and cyanide and to the polluted soil. Especially, it has high degradation activity to benzene.

The invention discloses a three-dimensional graphene-based nanometer metal composite material, a preparation method and application thereof. The three-dimensional graphene-based nanometer metal composite material comprises a graphene base body with a three-dimensional porous foam structure, and nano metal particles uniformly loaded on the graphene base body. The preparation method comprises the steps of: performing plasma processing on graphene in an air atmosphere, adding a soluble ferrite solution, uniformly mixing, then adding a reducing agent, and fully reacting under a set condition to obtain the three-dimensional graphene-based nanometer metal composite material. According to the three-dimensional graphene-based nanometer metal composite material, a three-dimensional graphene foam material is taken as a carrier, the nano metal particles uniformly coating the surface of graphene are prepared through a chemical liquid phase reduction method, the dispersibility of the nano particles is improved, the agglomeration is avoided; and meanwhile, the three-dimensional graphene-based nanometer metal composite material is generally of a three-dimensional porous structure, has the advantages of good stability, high degradation activity, high adsorption speed to polluted molecules, and the like, is more easily recovered, and has a wide application prospect in fields of chemical catalysis, sewage treatment and the like.

The invention relates to a method for preparing a micro magnetic carrier, the micro magnetic carrier and an active sludge immobilization method. The method for preparing the micro magnetic carrier comprises the following steps of: adding 10 to 50g / L of Fe3O4 particles into a polyvinyl alcohol solution, stirring, and adding a glutaraldehyde solution, concentrated hydrochloric acid and a sodium alginate solution for reacting; and washing with acetone, ethanol or distilled water, drying, and thus obtaining the micro magnetic carrier using the magnetic Fe3O4 particles as a core for wrapping a polyvinyl alcohol layer. A sequencing batch reactor technology is used in the active sludge immobilization method using the micro magnetic carrier as a core, the inoculation medium consists of the micro magnetic carrier and conventional sewage in a mass ratio of 1:1-1:3 and is used for secondary bio-treatment of active sludge, and the concentration of the inoculated active sludge is 2 to 4g / L (mass of the dry sludge / effective volume of a reactor); and the operating mode comprises water feeding, anaerobic stirring, aerobic aeration, settlement, drainage and standing. The invention has the advantages of quickening the biochemical reaction rate of dirt degradation and improving the treatment efficiency and the treatment effect of the sewage.

The invention relates to a method for preparing a semiconductor oxide catalyst which can degrade gas-phase volatile organic compounds. The method for preparing the semiconductor oxide catalyst with a synergistic effect of light and heat is characterized by comprising the following steps of: 1) selecting a semiconductor oxide and a solvent in a certain proportion of 0.1 to 1g: 10 to 40 ml, adding the semiconductor oxide into the solvent, and uniformly mixing the semiconductor oxide with the solvent to obtain a suspension of the semiconductor oxide; 2) placing a beaker which contains the suspension of the semiconductor oxide in an ultrasonic device, and performing ultrasonic treatment on the suspension for 1 to 2 hours; 3) coating the suspension on the surface of an ultraviolet lamp under the illumination of an infrared lamp; and 4) drying the surface of the ultraviolet light under the infrared lamp to obtain the semiconductor oxide catalyst with the synergistic effect of light and heat. The preparation method has the advantages of easily obtained raw materials, simple process and easy industrialization; and the semiconductor oxide catalyst prepared by the method can effectively degrade gas-phase volatile organic pollutants such as benzene, methyl benzene, methyl aldehyde, methyl alcohol, acetone and the like, and has excellent catalytic stability.

The invention provides a method for preparing a novel chicken manure organic fertilizer, comprising the following steps: (1) constructing a fermentation tank, forming vents in the bottom of the fermentation tank, and arranging a rainproof device on the top of the fermentation tank; (2) dehydrating chicken manure till the water content is 45-50%, mixing uniformly with straws, rice husk, humic acid, peanut shell, melon seed shell and bran to obtain a mixture, and uniformly spraying a compound fermentation agent to the mixture to obtain a fermented raw material; (3) transferring the fermented raw material obtained in step (2) to the fermentation tank constructed in step (1), piling up, and forming a plurality of vents penetrating through the pile by using a stick; and (4) turning over the fermented raw material once every day when the temperature of the pile reaches 35 DEG C or above and twice every day when the temperature of the pile reaches 60 DEG C or above, and curing, drying and crushing the pile when the temperature of the pile is lowered to 40 DEG C or below to obtain the chicken manure organic fertilizer.

The present invention belongs to the fields of microorganisms and applications thereof, and specifically discloses a composite micro-organism agent based on bacillus subtilis BS1 and an application thereof. The bacillus subtilis is preserved in China Center for Type Culture Collection and has a preservation number of CCCCC NO:M 2019185. The composite micro-organism agent prepared from the bacillussubtilis strain and saccharomyces cerevisiae, bacillus megaterium and bacillus licheniformis can be used for manure composting of livestock and poultry. The composite micro-organism agent can increase composting temperature in compost, shortens time to reach a high temperature, conducts deodorization, fixes nitrogen, and has significant reduction effects on various antibiotic resistance genes andpathogenic micro-organism in the compost.

The invention belongs to the technical field of inorganic nano composite materials, and discloses a preparation method of a visible light response SnIn4S8 nanoparticle / TiO2 nanobelt core-shell type composite photocatalyst. The preparation method comprises the following steps: firstly, dispersing TiO2 nanobelt into an ethylene glycol aqueous solution; sequentially adding tin chloride pentahydrate,indium chloride and thioacetamide; growing the snIn4S8 nanoparticles on the surface of the TiO2 nanobelt through a solvothermal method, and constructing the visible light response SnIn4S8 nanoparticle / TiO2 nanobelt core-shell type composite photocatalyst, which can be used for degrading methyl orange and tetracycline hydrochloride or photoreducing Cr (VI) under visible light. According to the composite photocatalyst constructed by the method, the light absorption range is widened, a photo-induced electron linear transmission path is provided, the separation efficiency of photo-induced electronpairs is improved, the active sites of the reaction are increased, and the photocatalytic efficiency and the stability are greatly improved. The method disclosed by the invention has the advantages of environment-friendly preparation raw materials, simple method, mild hybridization reaction conditions, short period, low cost and the like, and has a great application prospect in the aspect of water body pollution treatment.

The invention relates to a cultivation matrix, and especially relates to a purely natural organic nutritional matrix and a preparation method thereof. The purely natural organic nutritional matrix is characterized in that the matrix is mainly prepared through using 40-60 parts by weight of bark, 20-40 parts by weight of sawdust, 10-30 parts by weight of coal ash, 0.1-1 part by weight of a biological fertilizer starter, and 1-5 parts by weight of livestock manure. The organic nutritional matrix prepared in the invention has the advantages of low cost, abundant resources and stable quality, solves the waste utilization, energy saving and emission reduction problems, and also solves the nuisance-free matrix production problem by utilizing forest product wastes to produce a soilless nutritional matrix. The addition of a constant quality of a plurality of controlled-release nutritional master batches avoids burnt seedlings caused by the early-stage excess release of a fertilizer, satisfies the nutrition needs of the whole growth period of tree peony, and has a nutritional expiration period reaching above 120d.

The invention discloses a preparation method of micrococcus luteus immobilized beads. The method comprises the following steps: burying micrococcus luteus into a sodium alginate-porous soil filtering material mixed solution, so as to stably keep the activity of the micrococcus luteus; enabling the micrococcus luteus to grow into a source for providing resuscitation-promoting factor Rpf proteins for a long period, wherein the Rpf proteins can resuscitate and promote a potential functional bacterial group in a toxic and difficult-to-degrade organic pollution environment, so as to enhance efficient degradation of pollutants. The invention further provides application of the micrococcus luteus immobilized beads in the toxic organic pollutants; compared with common immobilized carrier powder active carbon, the operation cost is lower, and the micrococcus luteus immobilized beads have more remarkable economic benefits and environment-friendly benefits. The micrococcus luteus immobilized beads can be used for a biological treatment system of toxic and harmful, difficult-to-degrade and high-concentration organic industrial wastewater including coking wastewater, high-salt phenol-containing wastewater, printing and drying wastewater, pharmaceutical wastewater and the like.

The invention relates to incision difunctional alginate lyase Aly2 generating various monosaccharide products, an encoding gene of the Aly2 and an application of the Aly2. The amino acid sequence of the Aly2 is as shown in SEQ ID NO. 2, and the nucleotide sequence of the encoded Aly2 is as shown in SEQ ID NO. 1. The specific activity of prepared gene engineering recombinant protein rAly2 for alginate, polyguluronic acid and polymannuronic acid is 2025U / mg, 3672U / mg and 1324U / mg, so that the gene engineering recombinant protein rAly2 is a difunctional enzyme. The enzyme is an incision enzyme, and final main products are unsaturated disaccharide and unsaturated trisaccharide when an alginate polysaccharide substrate is thoroughly degraded. When different oligosaccharide substrates are degraded, various monosaccharide products such as saturated M and G or unsaturated delta can be generated. Besides, the rAly2 is stable in biochemical property, is a potential tool enzyme, can be applied tothe field of medicines, food and the like and has a wide application prospect.

The invention discloses alginate lyase and an application thereof, and belongs to the technical field of biology. The alginate lyase provided by the invention is high in degradation activity, and theenzyme activity can reach 65U / mg; the alginate lyase is stable in nature, and after the alginate lyase is stored at 4 DEG C for 18 months, the enzyme activity still maintains 98% or above of initial enzyme activity; and the alginate lyase has very high product specificity, and can specifically produce alginate oligosaccharides and alginate tri-saccharide. The prepared alginate lyase has importantindustrial application value and scientific research value.

The invention discloses an Ag / BiO2-x / Bi2O3 / Bi2O2.75 composite photocatalyst, a preparation method and application. NaBiO3 is added into a NaOH solution and then is dispersed to water to obtain a reaction precursor solution, and Bi2O2.75 / BiO2-x is prepared through hydrothermal reaction; the Bi2O2.75 / BiO2-x is dispersed in water, and stirring is performed under natural lighting condition to obtain turbid liquid A; AgNO3 is dispersed into the water to obtain a solution B; the solution B is dispersed into the turbid liquid A, and Ag / BiO2-x / Bi2O3 / Bi2O2.75 is obtained under natural lighting condition. The Ag / BiO2-x / Bi2O3 / Bi2O2.75 composite photocatalyst has oxygen vacancies, the synergistic resonance effect of the oxygen vacancies and surface plasmons of precious metals Ag makes the photocatalyst respond to a complete solar spectrum, and the photocatalyst is used for catalytic degradation of organic pollutants.

The invention provides a preparation method and application of a g-C3N4-@CeO2 composite catalyst, and the preparation method comprises the following steps: dispersing a graphite-phase carbon nitride monomer material in deionized water, and carrying out ultrasonic treatment to obtain a dispersion liquid I; dispersing a cerium dioxide monomer material in deionized water, and carrying out ultrasonictreatment to obtain a dispersion liquid II: dropwise adding the dispersion liquid I into the dispersion liquid II while stirring to obtain a mixed dispersion liquid; continuously stirring the obtainedmixed dispersion liquid to obtain a dispersion solution of a reaction product; and centrifuging and drying the reaction product to obtain the composite catalyst containing 5-35wt% of g-C3N4 by mass.The g-C3N4 / CeO2 composite catalyst material obtained by the invention has high room-temperature visible light irradiation activity and has an efficient catalytic degradation effect on formaldehyde, sothat the purpose of removing formaldehyde is achieved.

The invention belongs to the field of microorganisms, and particularly relates to a gossypol degradation strain coming from ruminant rumens and application thereof. The gossypol degradation strain is collected in a common microorganism center of the microorganism culture preservation and management committee of Chinese on August 23, 2013; and the culture preservation number is CGMCC No.8069. According to the gossypol degradation strain coming from ruminant rumens and the application, gossypol serves as a unique carbon source, the new strain with higher degradation activity to the gossypol is separated, screened, purified and cultivated from the ruminant rumens; through morphology and molecular biological identification, the strain RBS is Bacillus subtilis; and the homology between the Bacillus subtilis and the Bacillus tequilensis strain KM34 is 98%. The strain comes from the ruminant rumens of a living body, the nondeterminacy of the microorganism toxicology coming from soil or other natural environments is avoided, and a mice toxicity test shows that bacteria do not have toxicity. The liquid state fermentative gossypol degradation rate reaches 94.74%; after the bacteria are used for fermenting cottonseed meal, it is determined that the degradation rate of the free gossypol in the fermented cottonseed meal approaches to 80% through liquid chromatography (HPLC).

The invention discloses a Pd / TOC photocatalyst, a Pd / CdS / TOC photocatalyst, a preparation method and an application thereof. The Pd / TOC photocatalyst is prepared by taking TOC as a load object and loading Pd on TOC. The Pd / CdS / TOC photocatalyst is prepared by further loading CdS on the basis of the Pd / TOC photocatalyst. Various photocatalysts prepared according to the invention are all green and environment-friendly materials; no new pollution is generated after degradation of the photocatalysts; degradation efficiency is high; applicable environment is extensive; the photocatalysts can be used for treating wastewater; the efficiency of degrading 2,4-dichlorophenol is high; the popularization and application of photocatalysts have huge advantages.

The invention discloses a DSF quorum sensing signal molecule quenching bacterium and application thereof in plant disease prevention and treatment. Researches of the invention discover that Cupriavidus sp. can grow and propagate by using DSF as the only carbon source, nitrogen source and energy and has an evident degradation effect on high-concentration DSF. The Cupriavidus sp. strain HN-2 which is capable of efficiently degrading DSF is preserved in Guangdong Microbial Culture Collection Center on August 13th, 2018, and the preservation number of the strain is GDMCC No. 60432. The Cupriavidussp. strain HN-2 has evident degradation activity to quorum sensing signal molecules in phytopathogens, is environmentally friendly, has huge application potential in the prevention and treatment of DSF and / or DSF signal molecule analogue mediated phytopathogen, can reduce the problems of antibiotics abuse and pesticide residue pollution, can relieve environment pressure, provides a new thought for biological plant disease prevention and treatment, and has a huge application value and promising application prospect.

The invention discloses a DSF quorum sensing signal degrading bacteria, and applications thereof in plant disease control, and more specifically provides Burkholderia anthina bacterial strain HN-8 preserved at the Guangdong Culture Collection Center, on 24th, November, 2017, and the preservation number is GDMCCNO.60289. It is found in study that Burkholderia anthina, especially the bacterial strain HN-8 possesses remarkable and rapid degrading effect on quorum sensing signal molecule DSF, the degradation performance is stable, quorum sensing communication can be destroyed via degradation of DSF, and great application potential is achieved in prevention and treatment of black rotten caused by xanthomonas campestris, and pathogenic bacteria based on DSF. The Burkholderia anthina bacterial strain HN-8 also possesses relatively high drug resistance on ampicillin, carbenicillin, and streptomycin, so that pesticide or antibiotic abuse and drug resistance are avoided, and novel methods are provided for prevention of plant diseases.

The invention discloses a method for treating organic waste water with bentonite and ultrasonic waves. Granular or pulverized 10-100 mesh bentonite is added to the organic waste water, and the mass ratio of bentonite to waste water to be treated is 1:50-1:10000; Apply ultrasonic waves with a frequency of 20kHz-120kHz and a power of 100W-1200W for 10s-10min; the reaction product stays in the sedimentation tank for 10-60min, the soil-water settles and separates, and the waste water is discharged after reaching the standard. The waste water purification treatment time is greatly shortened, and the pollutant removal efficiency Improvement, simple operation, small equipment and low cost.

The invention relates to a crystal structure of thermophilic alphanol alcohol dehydrogenase under tail end degradation of thermophilic denitrified bacillus n-alkane. According to the crystal structure, the alcohol dehydrogenase is subjected to heterogenous expression by using escherichia coli E.coli; the pure alcohol dehydrogenase is obtained by a protein purification method; the crystal, proper for diffraction, of the alcohol dehydrogenase is obtained by a hanging-drop crystallizing method; and the crystal structure of the alcohol dehydrogenase is analyzed by an X-ray diffraction method. As the results shown, one subunit includes two structural domains, and one structural domain is separated by a crack; the N-end structural domain includes an Alpha / Beta fold similar to Rossman fold, and the structural domain also includes a binding site of NAD (Nicotinamide Adenine Dinucleotide) + coenzyme; the C-end structural domain includes an Alpha structural domain distribution similar to that of dehydro-quinate synthetase; and one iron ion is bonded to the C-end structural domain and exposed in the intermediate crack. According to the results, the spatial conformation of the alcohol dehydrogenase can be universally shown, and the theoretical guidance is provided for further searching the relationship between the structure and the function of the alcohol dehydrogenase and improving the degrading activity of the alcohol dehydrogenase.

The invention discloses a method for preparing a pure biomass organophosphorus pesticide degradation agent and belongs to the field of pesticide degradation agents. According to the method, a large number of organophosphorus pesticide degrading bacteria are cultured, separated and screened out from mixed soil of earth surface sludge, activated sludge and common earth surface soil; hypha suspension liquid, humus and chitosan are mixed and aged to obtain a pasty mixture; and the pasty mixture, nano titanium dioxide and a garlic extract concentrated solution are mixed and pelletized. The organophosphorus pesticide degrading bacteria screened and acclimatized through the method have high degradation activity, and the temperature and pH applicable range is wide; the pure biomass organophosphorus pesticide degradation agent is combined with substances such as humic acid and can degrade organophosphorus pesticide more thoroughly and faster; allicin and alliin in the garlic extract concentrated solution have a degradation effect on the organophosphorus pesticide; degradation of the pesticide can be accelerated through photocatalysis of the nano titanium dioxide; moreover, the prepared pure biomass degradation agent is non-toxic, has no residues and is free of secondary pollution.

The invention discloses a preparation method of raw starch-digesting glucoamylase and application of the raw starch-digesting glucoamylase to raw starch hydrolysis and preparation of ethanol by simultaneous saccharification and fermentation of raw starch. The raw starch-digesting glucoamylase is obtained after codon optimization, and a nucleotide sequence of the raw starch-digesting glucoamylase is shown as any of from A1) to A5): A1), sequence 3 in a sequence table; A2), nucleotides from 1st position to 1878th position in the sequence 3 in the sequence table; A3), DNA molecules shown by nucleotides in 19th position to 1878th position in the sequence 3 in the sequence table; A4), DNA molecules shown by nucleotides in 19th position to 1857th position in the sequence 3 in the sequence table; A5), a protein sequence shown in sequence 4 in a coding sequence table and showing 98% identity with the sequence limited by A1), A2), A3) or A4). Through experiments, it is proven that raw starch-digesting glucoamylase genes can be effectively expressed in a heterologous expression system to obtain the raw starch-digesting glucoamylase rPoGA15A, and the raw starch-digesting glucoamylase rPoGA15A and alpha-amylase act synergistically to hydrolyze the raw starch rapidly and efficiently and prepare the ethanol by simultaneous saccharification and fermentation of the raw starch.

The invention discloses a preparation method of a tin sulfide / vegetable sponge nanocomposite. The technical key lies in that a target product is obtained by carrying out a reaction at high temperatureby taking natural vegetable sponge as a carrier and petal-shaped tin sulfide as a photocatalyst. The preparation method has the advantages that: (1) the vegetable sponge is natural, green, pollution-free, wide in source, cheap and available; (2) the reaction condition is simple, the equipment requirement is low, and the energy consumption is low; (3) the product is good in catalytic effect, highin catalytic efficiency and strong in circularity; and (4) the preparation method is safe and smooth in reaction, free of naked fire or smoke and easy to realize industrial amplification.

The invention discloses a treatment method and application of antibiotic mushroom dregs. The method comprises the following steps: putting fresh antibiotic mushroom dregs into a reaction tank; carrying out uniform mixing under stirring, and then putting the formed mixture into a microwave reactor for microwave regulation and control; then uniformly mixing the mixture with a phanerochaete chrysosporium culture solution; carrying out filtering after standing for 1-2 days, collecting a solid part, and uniformly mixing the solid part with organic waste; and putting the formed mixture into a fermentation tank, and carrying out groove type aerobic composting fermentation. The treated antibiotic mushroom dregs can be used as a fertilizer due to high nitrogen content, can improve soil and does notpollute the environment. The method has good application prospects and can be applied to gardening, fruits and vegetables, farmlands, forests, urban landscaping lands and the like.

The invention belongs to the field of genetic engineering, and in particular relates to a resistance gene for degrading herbicide glyphosate and coded protein of the resistance gene. The gene and the protein are screened from a resistance gene which is capable of effectively degrading herbicide glyphosate; the gene is named glyphosate oxidase B4S7 gene, a nucleotide sequence is as shown in SEQ ID NO: 1, and the protein of the protein coded by virtue of the gene is as shown in SEQ ID NO: 2. Through DNA reshuffling on the coding gene of glycine oxidase mutant B3S1 of bacillus cereus as well as the enzyme-coupling activity screening based on T7 bacteriophage lytic-horseradish peroxidase / o-dianisidine, the glyphosate oxidase mutant B4S7 is finally obtained. The resistance gene disclosed by the invention preliminarily verifies the functions of the glyphosate oxidase B4S7 gene as well as application potential of the glyphosate oxidase B4S7 gene in glyphosate-resistance crops.