Gossypol degradation strain coming from ruminant rumens and application thereof

Abstract

The invention belongs to the field of microorganisms, and particularly relates to a gossypol degradation strain coming from ruminant rumens and application thereof. The gossypol degradation strain is collected in a common microorganism center of the microorganism culture preservation and management committee of Chinese on August 23, 2013; and the culture preservation number is CGMCC No.8069. According to the gossypol degradation strain coming from ruminant rumens and the application, gossypol serves as a unique carbon source, the new strain with higher degradation activity to the gossypol is separated, screened, purified and cultivated from the ruminant rumens; through morphology and molecular biological identification, the strain RBS is Bacillus subtilis; and the homology between the Bacillus subtilis and the Bacillus tequilensis strain KM34 is 98%. The strain comes from the ruminant rumens of a living body, the nondeterminacy of the microorganism toxicology coming from soil or other natural environments is avoided, and a mice toxicity test shows that bacteria do not have toxicity. The liquid state fermentative gossypol degradation rate reaches 94.74%; after the bacteria are used for fermenting cottonseed meal, it is determined that the degradation rate of the free gossypol in the fermented cottonseed meal approaches to 80% through liquid chromatography (HPLC).

Description

technical field [0001] The invention belongs to the field of microorganisms, in particular to a gossypol-degrading bacterial strain derived from the rumen of ruminants and an application thereof. Background technique [0002] With the development of animal husbandry, the shortage of protein feed in my country is becoming increasingly severe. For this reason, it needs to be solved by developing unconventional protein feed. Cotton meal has become the object of attention due to its protein content of 44%. Its protein content is second only to soybean meal. The annual output of cotton meal in my country is about 8 million tons, accounting for about 11% of domestic protein feed production. However, due to the anti-nutritional factors in cotton meal The presence, especially of free gossypol, hampers its widespread use in monogastric animal husbandry. [0003] Gossypol, commonly known as gossypol, is a polyphenol dinaphthalene derivative produced by the pigment glands of the genus...

AI Technical Summary

Problems solved by technology

It is generally believed that gossypol is toxic to ruminants. Although free gossypol in the rumen can be combined with soluble protein to reduce free gossypol entering the intestinal tract, 63% of gossypol still passes through the rumen, and the combined gossypol is in the rumen. The duodenum is again released, which makes it possible for gossypol ingested by ruminants to be absorbed like monogastrics

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