The invention discloses a method for evaluating the resistance of
pine tree calli to
bursaphelenchus xylophilus. The method is characterized in that the sterile
bursaphelenchus xylophilus is utilizedfor being inoculated to induced
pine tree embryogenic
cell lines, the responses of different
cell lines to the
bursaphelenchus xylophilus are compared through the appearance
shape change of the calli,a TTC cytochemical
staining result and a bursaphelenchus
xylophilus population increase speed, thus the
resistant cell lines are sifted out, the
sensitive cell lines are eliminated, and early-stage evaluation on the resistance of the
pine tree embryogenic cells to the bursaphelenchus
xylophilus can be conducted in the
callus stage. According to the method, the sterile bursaphelenchus
xylophilus is utilized for being inoculated to the induced pine tree embryogenic
cell lines, the responses of different cell lines to the bursaphelenchus xylophilus are compared through the appearance
shape change of the calli, the TTC cytochemical
staining result and the bursaphelenchus xylophilus
population increase speed, thus the
resistant cell lines are sifted out, the
sensitive cell lines are eliminated, and early-stage evaluation on the resistance of the pine tree embryogenic cells to the bursaphelenchus xylophilus can be conducted in the
callus stage. By means of the method, the resistance of thepine tree calli to the bursaphelenchus xylophilus is simply and quickly evaluated.