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87 results about "Bursaphelenchus xylophilus" patented technology

Bursaphelenchus xylophilus, commonly known as pine wood nematode or pine wilt nematode (PWN), is a species of nematode that infects pine trees and causes the disease pine wilt. It occurs in much of the United States, Canada, and Mexico. It also occurs in Japan, China, Taiwan, Korea, and Portugal.

Lycoris radiata bulb extractive pesticide and method for preparing extractive

Provided are a lycoris radiata bulb extractive pesticide and a method for preparing an extractive. Lycoris radiata bulbs can be manufactured into the lycoris radiata bulb extractive pesticide in forms of missible oil, microemulsion, wettable powder and the like through certain processing procedures. The lycoris radiata bulb extractive pesticide has good insecticidal ovicidal activities to tetranychus, plutella xylostella, vegetable aphides, armyworms and pine wood nematodes and protective effect in fields and woodlands. The pesticide is remarkable in effect, low in toxicity and residuals, simple in preparing process, low in cost and suitable for popularization and application and accords with the development tendency of existing pesticides, and pests do not easily produce drug resistance.
Owner:江西金帆达生化有限公司

Thiacloprid microcapsule powder agent, suspending agent and preparation method and application thereof

The invention relates to a control technology specially used for preventing and controlling important forest pest bursaphelenchus xylophilus (monochamus alternatus), namely a microcapsule powder agent or a microcapsule suspending agent prepared by a highly-efficient, harmfulless and less-persistent neonicotinoid insecticide thiacloprid sustained-release preparation and assisted by a high-adhesionadhesive is adopted for prevention and control. The technology adopts an innocuous and degradable carrier as shell materials, is safe to environment, has excellent durable effect and solves the greatproblems of bursaphelenchus xylophilus prevention and control and chemical pesticide pollution.
Owner:吴学民

Nematicidal composition and application thereof

The invention discloses a nematicidal composition and application thereof. The composition comprises active ingredients A and B, and a mass ratio of A to B is 20:1 to 1:20. The ingredient A refers to one of fosthiazate, abamectin, furfural, dazomet, fluopyram, carbosulfan, metham, verticillium chlamydosporium, emamectin benzoate, paecilomyces lilacinus or fluensulfone; and the structural formula of the ingredient B is a formula I or a formula II, wherein the formula I is as shown in the specification, and the formula II is as shown in the specification. The nematicidal composition disclosed by the invention is used for killing plant parasite nematodes comprising root-knot nematodes, ditylenchus destructor, heterodera sachachtii, pratylenchus coffeae and bursaphelenchus xylophilus, is composed of a novel nematicide containing lactonic rings and the existing insecticides and bactericides, and has good nematocidal activity and low toxicity to people and livestock. The nematicidal composition disclosed by the invention has an excellent effect of controlling eggs and second-stage larvae of the root-knot nematodes.
Owner:SHANDONG UNITED PESTICIDE IND CO LTD

Application of ethyl palmitate in preventing and controlling pine wilt disease

The invention relates to application of ethyl palmitate in preventing and controlling pine wilt disease. A method provided by the invention for preventing and controlling the pine wilt disease by using ethyl palmitate is that: the ethyl palmitate is placed into pine trees which contain generative bursaphelenchus xylophilus. The method provided by the invention can be used for treating pine trees which are infected with bursaphelenchus xylophilus in a forest. The problems of mass reproduction and rapid diffusion of bursaphelenchus xylophilus under a bursaphelenchus xylophilus epidemic situation can be solved.
Owner:INST OF ZOOLOGY CHINESE ACAD OF SCI

Bacterial agent capable of killing nematode and application thereof

The invention relates to nematocide and its appliances thereof, belonging to the field of biological pesticide technique. Said nematocide is acquired through the sequence of culturing preparing strain in test tube, fluid enlargement culture and extracting nematocide active component, and said preparing strain is as follows: germinated barley-acceptable single cell bacteria stenotrophomonas maltophilia G6 which is preserved in microbe bacterial preservation management commission common microbe center of China with preservation data: 2005-10-31 and preservation registration number CGMCC No: 1522. The main mechanism of action of G6 strain is to break up body wall of worm through producing enzyme and poisoning worm, especially pine beam worm, and could be used to prepare biological nematocide. The production in said invention is characterized on high poison ability, which has better potential utility.
Owner:YUNNAN UNIV

Method for detecting bursaphelenchus xylophilus, detection primer and LAMP (loop-mediated isothermal amplification) detection kit of detection primer

The invention discloses a method for detecting bursaphelenchus xylophilus, a detection primer and a LAMP (loop-mediated isothermal amplification) detection kit of the detection primer. The method for detecting the bursaphelenchus xylophilus comprises the steps of (1) extracting a DNA (deoxyribonucleic acid) of the bursaphelenchus xylophilus in a pine wood sample; (2) constructing a LAMP reaction system for loop-mediated isothermal amplification; (3) judging whether the bursaphelenchus xylophilus exists in the pine wood sample according to the change of fluorescence or turdity of an amplified product. The invention also provides a method which is easy to operate, low in cost and high in stability and is used for extracting the DNA of the bursaphelenchus xylophilus from pine wood. The invention further provides a LAMP primer group with high specificity and high sensitivity for detection of the bursaphelenchus xylophilus. The invention also provides the LAMP detection kit for the bursaphelenchus xylophilus. The LAMP detection kit comprises the LAMP primer group, a reaction buffering solution, a DNA chain replacement enzyme and a fluorescent dye / specific probe. The detection kit disclosed by the invention has the advantages of high specificity, high sensitivity, convenience and quickness in detection and the like.
Owner:杭州益森键生物科技有限公司

Method for treating dead and drying tree suffering from pine wood nematode disease

InactiveCN102428907AEasy to handleEfficient and safe handlingAnimal huntingInsect diseaseTree trunk
The invention relates to the field of national serious pest comprehensive treatment. In a method for treating a dead and drying tree suffering from the pine wood nematode disease, for falling the dead and drying tree suffering from the pine wood nematode disease in place, the dead and drying tree suffering from the pine wood nematode disease is covered with woodland dry branches and fallen leaves and / or weed shrubs and / or soil. Under the condition with thick woodland soil layer, a soil pit can be dug, the trunk and the dead and drying lateral branches with the diameters of over 1cm of the dead and drying tree suffering from the pine wood nematode disease are placed into the soil pit and are covered with the soil and / or the woodland dry branches and fallen leaves and / or the weed shrubs. In the method, by utilizing an ecology principle, entomopathogens in the dead and drying tree suffering from the pine wood nematode disease are artificially induced to attack monochamus alternatus hope and soil termites are induced to consume substrates on which the monochamus alternatus hope and pine wood nematode depend for living. The invention provides the method for treating the dead and drying tree suffering from the pine wood nematode disease, which is convenient, simple, easy, efficient and safe.
Owner:HUIZHOU CITY SHEN LONG COMPREHENSIVE MANAGEMENT OF PINE WILT DISEASE CO LTD

Bursaphelenchus xylophilus invasion risk prediction method based on ecological niche factor model

The invention belongs to the technical field of bursaphelenchus xylophilus invasion risk prediction, and discloses a bursaphelenchus xylophilus invasion risk prediction method based on an ecological niche factor model, and the method comprises the steps: obtaining distribution point data and environment data of an epidemic area of bursaphelenchus xylophilus; performing Kriging interpolation processing on the climate data, calculating the gradient and the slope direction in the topographic data, and obtaining vegetation types and human activity interference data; importing the distribution point data and various environmental data into an ecological niche factor model, and calculating pine wood nematode disease risk early warning; and evaluating the pine wood nematode disease invasion riskbased on the feature matrix and the risk map obtained by calculation. According to the invention, main environmental influence factors influencing pine wood nematode propagation are used; the method is suitable for local conditions, lays a foundation for early warning, monitoring and control work of pine wood nematode diseases and insect pests, provides a scientific basis for measures of forest biodiversity protection and ecological environment protection, and provides a basis and a reference for early warning and control work of forest diseases and insect pests.
Owner:YANGTZE UNIVERSITY

Candida with nematocidal activity as well as preparation method and application of candida

The invention provides Candida with a nematocidal activity as well as a preparation method and application of Candida and belongs to the technical field of microbial pesticides. The Candida is Candida krusei TY9 and is preserved in the typical culture preservation center in China, and the preservation number of the Candida is CCTCCNO: M2014435. The Candida krusei TY9 strain is prepared by fermentation culture of conventional liquid, is applied to prevention and control of plant-parasitic nematodes, and particularly has a good effect of preventing and controlling panagrellus redivivus and / or bursaphelenchus xylophilus. The Candida has the advantages of low cost, simple preparation process, easiness in raw material obtaining, good in effect of killing nematodes and the like, and is suitable for industrial popularization and application.
Owner:NANYANG NORMAL UNIV

Pesticide for controlling monochamus alternatus and application method thereof

The invention belongs to the field of agriculture production, aims at the monochamus alternatus causing the pine wilt disease, and discloses a pesticide for controlling monochamus alternatus and an application method thereof. The pesticide is prepared by mixing main materials namely thiamethoxam and phorate. The pesticide is painted on the bark of pine trees, is injected into the pine trees, or is sprayed on the soil so as to effectively control the monochamus alternatus. The provided pesticide has the advantages of simple formula and user-friendliness, can prevent the propagation and generation of pine wilt disease by killing the monochamus alternatus, and is used to control pine wilt disease in pine planting.
Owner:FUJIAN AGRI & FORESTRY UNIV

Rapid culture method of Bursaphelenchus xylophilus endoparasitic fungus Esteya vermicola

The invention discloses a rice medium formula and culture method of Bursaphelenchus xylophilus endoparasitic fungus Esteya vermicola, and belongs to the technical field of microorganisms. Compared with a conventional PDA solid culture method and a PDB liquid culture method, a rice medium preparation and culture method of the Bursaphelenchus xylophilus endoparasitic fungus Esteya vermicola not onlyshortens the culture time of the bacteria, but also obtains a high-purity, high-density fungal spore suspension. The method is applied to the control of Bursaphelenchus xylophilus, and has significant economic, ecological and social benefits.
Owner:SOUTH CHINA AGRI UNIV

Protein BxCDP1 of bursaphelenchus-xylophilus pathogen-associated molecular patterns and application thereof

The invention discloses protein BxCDP1 of bursaphelenchus-xylophilus pathogen-associated molecular patterns and application thereof. The amino acid sequence of the protein BxCDP1 of the pathogen-associated molecular patterns (PAMPS) is shown in SEQID NO.2. From effectors secreted by bursaphelenchus xylophilus and the PAMP, the defensive responses of a host plant, namely, a pine tree, to bursaphelenchus xylophilus invasion are studied, the protein BxCDP1 of the pathogen-associated molecular patterns is obtained from the bursaphelenchus xylophilus, it is proven through experiments that the protein BxCDP1 can trigger cell necrosis of various plants including the host plant, has a certain broad spectrum in cell necrosis triggering, and stimulates the defensive response of the host plant. The BxCDP1 triggered cell necrosis depends on a co-receptor BAK1 of pattern recognition receptors, the BxCDP1 can stimulate the accumulation of nicotiana benthamiana ROS and up regulation of PTI Marker genes, and the immunoreaction of the nicotiana benthamiana is stimulated. It can be seen that the BxCDP1 is a PAMP secreted by the bursaphelenchus xylophilus, and has important theoretical and practicalsignificance for revealing the pathogenic mechanism of the bursaphelenchus xylophilus and improving the resistance of the pine tree to the bursaphelenchus xylophilus in a targeted mode.
Owner:NANJING FORESTRY UNIV

LAMP (Loop-mediated Isothermal Amplification) primer group, kit and detection method for detecting bursaphelenchus xylophilus nickle

The invention discloses a LAMP (Loop-mediated Isothermal Amplification) primer group, a kit and a detection method for detecting bursaphelenchus xylophilus nickle. According to the invention, firstly, a conserved SYG-2 gene in the bursaphelenchus xylophilus nickle is used as a target gene, the LAMP primer group with high specificity for bursaphelenchus xylophilus nickle is designed and screened out, and the LAMP primer group can specifically distinguish bursaphelenchus xylophilus nickle from bursaphelenchus mucronatus. The invention utilizes the LAMP primer group to establish the bursaphelenchus xylophilus nickle LAMP rapid detection kit. The invention further utilizes the LAMP primer group to establish the bursaphelenchus xylophilus nickle LAMP rapid detection method. According to the method, whether bursaphelenchus xylophilus nickle is carried in a sample or not can be accurately distinguished. The LAMP detection method disclosed by the invention is high in specificity, simple and convenient to operate and short in time consumption, and has the important significance for rapid detection on bursaphelenchus xylophilus nickle carried in monochamus alternatus and total early warning and a control strategy for the pine wilt disease.
Owner:INST OF FOREST ECOLOGY ENVIRONMENT & PROTECTION CHINESE ACAD OF FORESTRY

Method for storing monochamus alternatus hope sample and method for fast detecting bursaphelenchus xylophilus therein

The invention discloses a method for storing a monochamus alternatus hope sample and a method for fast detecting bursaphelenchus xylophilus therein. The method comprises the following step of putting the caught monochamus alternatus hope sample into 75% ethyl alcohol or a refrigeratory with -70 DEG C to be stored, thereby furthest preserving the amount of the bursaphelenchus xylophilus carried bythe monochamus alternatus hope. The invention creates a production technology of the bursaphelenchus xylophilus DNA template in the monochamus alternatus hope with the storing state, directly freezesand thaws lyses and centrifuges the middle and back thoracic tracheal tissues of the adult monochamus alternatus hop for PCR amplification, has the amplification rate of 100%, fastens the detecting speed, improves the detecting accuracy, and achieves the aim of convenient, fast and accurate detection.
Owner:SOUTH CHINA AGRI UNIV

Emamectin benzoate pesticide capable of preventing Bursaphelenchus xylophilus

The invention discloses an emamectin benzoate pesticide for preventing Bursaphelenchus xylophilus. The preparation raw material comprises: 1.5-2.5 wt% of emamectin benzoate, 5-20wt% of compound containing oxyethylene group, 5-57wt% of monohydric alcohol with 1-6 carbon atoms, 0.1-0.5 wt% of thickener, 2-20wt% of alcohol ether compound and the balance of water. The emamectin benzoate pesticide agent has excellent stability through reasonable composition, and the extremely fine particle size ensures that the agent can be stored for a long time. The efficacy test proves that the agent has remarkable effect on preventing Bursaphelenchus xylophilus and has broad development prospects.
Owner:宁波甬森生物科技有限公司

Universal detection primer and DNA barcode molecular identification method for bursaphelenchus xylophilus and similar species thereof

The invention discloses a universal detection primer and a DNA barcode molecular identification method for bursaphelenchus xylophilus and similar species thereof. A forward primer is represented as AGTACCGTGAGGGAAAGTTGA and a reverse primer is represented as GCTACTAGATGGTTCGATTAGTCT; the universal primer can be used for amplifying for D2 / D3 in a ribosomal 28S gene of the bursaphelenchus xylophilus, sequencing an amplified product, comparing the detected sequence with an established sequence standard database of the bursaphelenchus xylophilus and similar species thereof, and rapidly and accurately identifying the bursaphelenchus xylophilus and similar species thereof depending on the differences of DNA sequence of the 28S; and the universal detection primer and the DNA barcode molecular identification method have extensive using value in such aspects as exit and entry plant quarantine, domestic forestry quarantine and forest nematode disease investigation.
Owner:NINGBO ACAD OF SCI & TECH FOR INSPECTION & QUARANTINE

Method for ultralow-temperature preservation of anti-bursaphelenchus-xylophilus pinus massoniana embryonic callus

The invention belongs to the technical field of tissue culture and discloses a method for ultralow-temperature preservation of anti-bursaphelenchus-xylophilus pinus massoniana embryonic callus. By proper pretreatment and adoption of a mixed cryoprotectant, the method is suitable for freeze preservation of the anti-bursaphelenchus-xylophilus pinus massoniana embryonic callus, cell viability of theembryonic callus unfrozen at 30-35 DEG C reaches 100% maximally, and the embryonic callus subjected to ultralow-temperature preservation has no evident difference from embryonic callus subjected to normal proliferation in appearance and microstructure and still has a function of differentiation for forming somatic embryos. An ultralow-temperature preservation system of the anti-bursaphelenchus-xylophilus pinus massoniana embryonic callus is established preliminarily, and technical references are provided for long-time embryonic maintaining of the resistant pinus massoniana embryonic callus.
Owner:NANJING FORESTRY UNIV

PCR-RFLP method for identifying mucronate form of Bursaphelenchus xylophilus and round-tailed form of Bursaphelenchus xylophilus

The invention discloses a PCR-RFLP (polymerase chain reaction- restriction fragment length polymorphism) method for identifying a mucronate form of Bursaphelenchus xylophilus and a round-tailed form of Bursaphelenchus xylophilus. The method comprises the following steps: DNA extraction, PCR amplification, reaction with restriction endonucleases HhaI and Hpy188I and spectral identification. If themaximum band of the PCR-RFLP spectrum resulting from the reaction with the restriction endonuclease HhaI is 467 bp, the mucronate form of Bursaphelenchus xylophilus is identified; if the maximum bandis 357 bp or 358 bp, the reaction with restriction endonuclease Hpy188I is carried out; and if the maximum band is 492 bp, the mucronate form of Bursaphelenchus xylophilus is identified. If the maximum spectral band is 743 bp or 741 bp, the round-tailed form of Bursaphelenchus xylophilus is identified. Therefore, the PCR-RFLP method can clearly identify the mucronate form of Bursaphelenchus xylophilus and the round-tailed form of Bursaphelenchus xylophilus and provides a guarantee for quarantine identification and treatment.
Owner:宁波中盛产品检测有限公司 +1

Primers, kits and method for fluorescent quantitative PCR detection of bursaphelenchus xylophilus or bursaphelenchus mucronatus

The invention discloses primers, kits and a method for fluorescent quantitative PCR detection of bursaphelenchus xylophilus or bursaphelenchus mucronatus, wherein the fluorescent quantitative PCR detection primer for bursaphelenchus xylophilus comprises a PCR amplification primer I; the PCR amplification primer I comprises a PCR amplification forward primer I with the nucleotide sequence shown inSEQ ID NO: 1 (5'-GTTCCGCCTACTGATGGTTC-3') and a PCR amplification reverse primer I with the nucleotide sequence shown in SEQ ID NO: 2 (5'-CGGCTTTTCATCATCCTACGC-3'); the fluorescent quantitative PCR detection primer for bursaphelenchus mucronatus comprises a PCR amplification primer II; and the PCR amplification primer II comprises a PCR amplification forward primer II with the nucleotide sequenceshown as SEQ ID NO: 4 (5'-TTCTCTACGCAGCACGTTTGTCCG-3') and a PCR amplification reserve primer II with the nucleotide sequence shown as SEQ ID NO: 5 (5'-TTCACGAGACAGAGCTCACAACAAA-3'). The invention hasthe advantages of good DNA extraction effect, simple operation, short time consuming (within 1 hour), accurate and reliable results, and at least a single nematode can be detected, with the characteristics of high sensitivity and high specificity.
Owner:沈阳知物检测技术有限公司 +3

Primer group and kit for detecting whether monochamus alternatus carries Bursaphelenchus xylophilus Nickle and application of primer group

The invention discloses a primer group and a kit for detecting whether monochamus alternatus carries Bursaphelenchus xylophilus Nickle and application of the primer group. The invention first discloses the LAMP primer group for detecting whether the monochamus alternatus carries the Bursaphelenchus xylophilus Nickle. With an SYG-2 gene of the Bursaphelenchus xylophilus Nickle as a target gene, the LAMP primer group with high specificity to the Bursaphelenchus xylophilus Nickle is designed and screened; and the LAMP primer group can specifically distinguish the Bursaphelenchus xylophilus Nickle from Bursaphelenchus mucronatus. The invention further discloses the LAMP detection kit for detecting whether the monochamus alternatus carries the Bursaphelenchus xylophilus Nickle. The LAMP detection kit comprises the LAMP primer group. The invention further establishes an LAMP detection method for detecting whether the monochamus alternatus carries the Bursaphelenchus xylophilus Nickle by using the LAMP primer group. By the LAMP detection method, quick and accurate detection on the Bursaphelenchus xylophilus Nickle carried by the monochamus alternatus can be achieved; the LAMP detection method is high in specificity, easy and convenient to operate and low in cost, and is of a great significance in monitoring occurrence and spread of a pine wilt disease.
Owner:INST OF FOREST ECOLOGY ENVIRONMENT & PROTECTION CHINESE ACAD OF FORESTRY

Monochamus alternatus repellent and preparation method thereof

The invention discloses a monochamus alternatus repellent. The repellent comprises in percent by weight: 10-30% of p-allylanisole, 60-80% of a solvent and 5-10% of a synergist. The invention also discloses a preparation method of the monochamus alternatus repellent. The beneficial effects are as follows: the monochamus alternatus in the pine forest can be significantly repelled by the synergisticaction of the active components with good repelling effects, the carrying and spreading of bursaphelenchus xylophilus is reduced, the survival rate of pine trees is improved, the flooding of bursaphelenchus xylophilus can be effectively prevented and treated, and the transmission path is cut off. The biological prevention and treatment concept is used, the preparation of the repellent is simple and convenient, the cost is low, the resistance to monochamus alternatus cannot be caused for the long-term use, and thus the repellent is suitable for the hanging and slow-release use in a large area in the pine forest.
Owner:南京生兴有害生物防治技术股份有限公司

Method for efficiently trapping Monochamus alternatus and special device for method

The invention discloses a method for efficiently trapping Monochamus alternatus and a special device for the method. According to the method, traps are installed in a Monochamus alternatus region, and the Monochamus alternatusis trapped by 495-500nm of light waves emitted from the traps. According to the method for efficiently trapping the Monochamus alternatus and the special device for the method, disclosed by the invention, the Monochamus alternatus is trapped by adopting monochromatic LED (light-emitting diode) illuminators, so the trapping efficiency is high; the method has a very good trapping effect on the Monochamus alternatus in a diet supplement period and a spawning period, not only is capable of reducing the population number of the Monochamus alternatus and monitoring the population dynamics of the Monochamus alternatus but also is suitable for preventing the propagation of Bursaphelenchus xylophilus to reduce the hazard of the Bursaphelenchus xylophilus.
Owner:NANJING FORESTRY UNIV

Method for evaluating resistance of pine tree calli to bursaphelenchus xylophilus

InactiveCN109924129AQuick judgment of resistanceEase of evaluationHorticulture methodsPlant tissue cultureShape changeCytochemical staining
The invention discloses a method for evaluating the resistance of pine tree calli to bursaphelenchus xylophilus. The method is characterized in that the sterile bursaphelenchus xylophilus is utilizedfor being inoculated to induced pine tree embryogenic cell lines, the responses of different cell lines to the bursaphelenchus xylophilus are compared through the appearance shape change of the calli,a TTC cytochemical staining result and a bursaphelenchus xylophilus population increase speed, thus the resistant cell lines are sifted out, the sensitive cell lines are eliminated, and early-stage evaluation on the resistance of the pine tree embryogenic cells to the bursaphelenchus xylophilus can be conducted in the callus stage. According to the method, the sterile bursaphelenchus xylophilus is utilized for being inoculated to the induced pine tree embryogenic cell lines, the responses of different cell lines to the bursaphelenchus xylophilus are compared through the appearance shape change of the calli, the TTC cytochemical staining result and the bursaphelenchus xylophilus population increase speed, thus the resistant cell lines are sifted out, the sensitive cell lines are eliminated, and early-stage evaluation on the resistance of the pine tree embryogenic cells to the bursaphelenchus xylophilus can be conducted in the callus stage. By means of the method, the resistance of thepine tree calli to the bursaphelenchus xylophilus is simply and quickly evaluated.
Owner:NANJING FORESTRY UNIV

Bursaphelenchus xylophilus disease image recognition and detection method and device

The invention belongs to the technical field of pine wood nematode disease image recognition and detection, and particularly relates to a pine wood nematode disease image recognition and detection method and a pine wood nematode disease image recognition and detection device, pine wood nematode disease is detected by setting a deep learning target detection technology, the recognition efficiency of diseased wood can be effectively improved, and the detection precision is relatively high; the image intelligent identification and positioning method adopts a unified discrimination standard, so the coverage rate of identification results is effectively improved, and the generalization ability is high. According to the above advantages, the pine wood nematode disease image identification and detection method can timely discover infected pine trees and determine the distribution condition of the pine trees, effectively monitor the development trend of pine wood nematode disease epidemic situations, and provide timely and accurate information for pine forest management personnel and forest protection personnel.
Owner:NORTHEAST FORESTRY UNIVERSITY

Comprehensive treatment method for controlling bursaphelenchus xylophilus disease epidemic situation not to spread

InactiveCN113455261APrevent proliferationAvoid preventive measuresPlant protectionDiseaseNonwoven fabric
The invention discloses a comprehensive treatment method for controlling the bursaphelenchus xylophilus disease epidemic situation not to spread, and relates to the technical field of comprehensive prevention and control over bursaphelenchus xylophilus. The invention relates to a comprehensive treatment method for controlling the bursaphelenchus xylophilus disease epidemic situation not to spread. The method comprises the following steps: regularly patrolling the ground; removing dead trees and dead branches in time: cutting all dead trees and dead branches before eclosion of adult longhorn beetles, carrying out chemical fumigation, pesticide spraying and bagging cleaning work on stumps, the dead trees and the dead branches, and carrying out strict acceptance inspection; carrying out fixed monitoring of traps: hanging live monochamus alternatus traps as fixed monitoring points; arranging trap trees: selecting small-diameter pinus massoniana and pinus thunbergii around the trapper, carrying out girdling on tree trunks 50 cm away from the ground, and adding attractants; releasing sclerodermus harmandi; and hanging a beauveria bassiana non-woven fabric. According to the comprehensive treatment method for controlling the bursaphelenchus xylophilus disease epidemic situation not to spread, a single prevention and control measure is avoided, the prevention and control effect of bursaphelenchus xylophilus is improved, epidemic situation recurrence is avoided, and the treatment time is shortened.
Owner:安徽省友林林业技术发展有限公司

DNA extraction method for bursaphelenchus xylophilus from wood chips, lamp primer set for bursaphelenchus xylophilus, and detection method for bursaphelenchus xylophilus from wood chips

To extract and detect Bursaphelenchus xylophilus DNA without isolating Bursaphelenchus xylophilus from a piece of wood taken from a tree belonging to the genus Pinus and the like, provided are a method for extracting Bursaphelenchus xylophilus DNA from a collected piece of wood that includes Bursaphelenchus xylophilus, a LAMP primer set including primers that anneal to a specific region of Bursaphelenchus xylophilus DNA, and a method for detecting Bursaphelenchus xylophilus from a piece of wood by amplifying a DNA by a LAMP method using this primer set.
Owner:FORESTRY & FOREST PRODS RES INST

Bursaphelenchus xylophilus comprehensive prevention and control command device

InactiveCN113519476AReduce labor intensityPlay the role of integrated pest controlInsect catchers and killersTrappingAgricultural engineering
The invention discloses a bursaphelenchus xylophilus comprehensive prevention and control command device. The device comprises a base, a U-shaped frame is fixedly installed in the middle of the upper end of the base, fixing plates are fixedly installed on the left portion and the right portion of the upper end of the U-shaped frame correspondingly, and sliding grooves are formed in the opposite faces of the two fixing plates correspondingly. Sliding devices are arranged on the groove walls of the two sliding grooves, a groove is formed in the rear portion of the upper end of the base, a sliding clamping device is arranged on the groove wall of the groove, trapping boxes are fixedly installed at the upper ends of two concentric-square-shaped frames, and the inner cavity and the upper end of each trapping box is commonly provided with a trapping device. The inner cavities of the two concentric-square-shaped frames are each provided with an expansion extrusion device, and the lower ends of the two concentric-square-shaped frames are each provided with a pesticide spraying device. According to the bursaphelenchus xylophilus comprehensive prevention and control command device, the pesticide spraying mode and the trapping and killing mode are used for achieving the comprehensive prevention and control effect of insect killing, the prevention and control comprehensiveness is improved, and then the prevention and control effect is improved.
Owner:安徽省友林林业技术发展有限公司

Bursaphelenchus xylophilus effector BxSCD1 interaction protein as well as coding gene and application thereof

The invention discloses a pine wood nematode effector BxSCD1 interaction protein as well as a coding gene and application thereof, and belongs to the technical field of molecular biology. The pine wood nematode effector BxSCD1 interaction protein has an amino acid sequence as shown in SEQ ID No. 1, and a nucleotide sequence for expressing the protein is as shown in SEQ ID No. 2. Starting from an effector secreted by the pine wood nematode and a host target, the interaction between a host pine tree and the pine wood nematode is studied, it is found that BxSCD1 silencing can delay the disease attack of the black pine, and BxSCD1 inhibits expression of genes related to the disease course of the black pine; through interaction of the BxSCD1 and the pine tree ethylene synthase gene ACO1, it is proved that the BxSCD1 can influence the immune response of the pine tree by influencing the expression quantity of the ethylene synthase ACO1 of the black pine, and the BxSCD1 has important scientific significance in the aspects of revealing the pathogenesis of the pine wood nematode, early detection of the occurrence of the pine wood nematode disease and the like.
Owner:NANJING FORESTRY UNIV

Bursaphelenchus xylophilus disease occurrence area intelligent identification method

The invention discloses a pine wood nematode disease occurrence area intelligent identification method. Remote sensing image data and a deep learning technology are applied to the field of pine wood nematode disease monitoring. A pine wood nematode disease occurrence area semantic segmentation sample data set is constructed based on a remote sensing image, a UNet semantic segmentation model is constructed, the model is trained and optimized, and intelligent recognition of a pine wood nematode disease occurrence area is achieved. The method is simple in process and high in practicability, a new method is provided for intelligent monitoring of pine wood nematode diseases, and the method is suitable for the fields of forest pest and disease damage monitoring, deep learning image recognition and the like.
Owner:国家林业和草原局生物灾害防控中心

Morphological and molecular combined detection method for pine wood nematodes

The invention belongs to the technical field of morphological and molecular combined detection, and discloses a morphological and molecular combined detection method for pine wood nematodes. The method comprises the steps of sample separation, morphological detection of the pine wood nematodes and molecular detection of the pine wood nematodes. A simple pine wood nematode separation device is designed in the invention; saprophytic nematodes and aphelenchoides nematodes are distinguished by adopting the presence or absence of stylets, and morphological identification is carried out according tothe shape difference of the tails and the tips of the female adults of pine wood nematodes and the quasi pine wood nematodes; molecular identification is carried out on the aphelenchoides nematodes,the pine wood nematodes and bursaphelenchus mucronatus by adopting a specific PCR technology; and complete detection technology system details are designed. The same pine wood sample is repeatedly sampled for three times during DNA extraction, so that accidental detection errors during sampling are avoided. The method meets requirements of forestry workers for rapid, efficient and accurate pine wood nematode detection, and provides timely and reliable detection means and technical support for general investigation of pine wood nematode diseases of forestry departments.
Owner:CHONGQING NORMAL UNIVERSITY
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