48 results about "Tetranychus" patented technology

The invention belongs to the technical field of pesticide and midbody synthesis, particularly relates to a synthesis method for bifenazate. In the synthesis method for the bifenazate, a target product is synthetized by taking para-hydroxydiphenyl as a raw material through nitration, methylation, hydrogenated reduction, diazotization, diazonium salt reduction and an acylation one-step method, wherein acylation reaction is directly performed on hydrazonium salt without treatment, thus, the experimental procedure is shortened, the yield is improved to above 65 percent from 50 percent, the conversion rate of a reaction product is high, and the purification treatment is easy to perform. The synthesis method for the bifenazate has the advantages that the operation is simple, the condition is mild, the yield is high, the cost is low, and the like, is easy to carry out industrial production and has a wide application prospect. The bifenazate synthetized by the invention can be used as a specific acaricide, is active in active stage of tetranychid, can also control pests, such as Tetranychus urticae, in the egg stage, and is used for cotton, hop and certain fruiter crops to effectively prevent all mites in the growth stage.

The invention relates to a chalcone compound derived from Beta-ionone and a preparation method and an application thereof. The preparation method for the compound specifically comprises the followingsteps: in an organic solvent, and in the presence of a trapping agent, performing a condensation reaction on the Beta-ionone and substituted cinnamyl aldehyde, to obtain the compound. The provided chalcone compound derived from the Beta-ionone has apparent attractive activity of apolygus lucorum and insecticidal activity of plutella xylostella and tetranychus cinnabrinus, and has the direct control effect to the apolygus lucorum, the plutella xylostella and the tetranychus cinnabrinus. In addition, the preparation method is simple in process, easy to implement, less in by-product, higher in yield, and low in cost, and has a practical application value in agricultural production, and extensive application prospect.

The invention belongs to the technical field of compounding pesticides, and specifically relates to missible oil containing flonicamid and bifenthrin and an application of the missible oil. The effective components of the missible oil are flonicamid and bifenthrin in a weight ratio of 20:1-1:1. The missible oil containing flonicamid and bifenthrin is particularly beneficial to prevention and treatment of tetranychus telarius linne by virtue of synergistic interaction effects of the components.

The invention relates to an acaricidal composition, in particular to an acaricidal composition containing spirodiclofen and cyflumetofen, which is particularly used for preventing panonychus citri, European red mites, European red two-spotted spider mites and cotton leaf mites. The acaricidal composition has an obvious synergistic effect on mite killing as compared with a single pesticide, can kill egg, larva, nymph and adult of acarid, and is contributed to postponing the generation of pesticide resistance of bacteria.

The invention discloses a sequence characterized amplified region (SCAR) marker of tetranychus truncates ehara, a specific PCR detection method and a kit of tetranychus truncates ehara. The SCAR marked nucleotide sequence is as set forth in SEQ ID NO.1. The specific PCR detection method is characterized by that: a specific primer used in the detection is designed by the SCAR marked sequence, and a target amplified region is on the SCAR marked sequence. The specific PCR detection method and the kit of tetranychus truncates ehara provided by the invention have the advantages of strong specificity, high detection efficiency, good operability and the like, thus being capable of being widely used as a low-cost detection technology of tetranychus truncates ehara.

The invention relates to a semi-artificial diet preparing and breeding method of tetranychus cinnabarinus. The method is characterized in that: any four or more than four of saccharose, beer yeast powder, fresh yolk, multi-vitamins, KH2PO4 and Na2HPO4 are taken to form a mixture of more than 10 g and less than 30 g, then 164 g of water, 0.005 g of streptomycin sulphate and 0.2 g of sorbic acid are added into the mixture, the volume is metered to 200 ml, then 30 ml of an aqueous liquid ofkidney beanleavesis added to form a semi-artificial diet for the tetranychus cinnabarinus, and the semi-artificial diet is used in the method for breeding the tetranychus cinnabarinus.A novel liquid is provided for the maintenance and amplification of the tetranychus cinnabarinuspopulation, the tetranychus cinnabarinuspopulation is maintained and reproduces within a certain period of time, and particularly, the method is used to store the food for thetetranychus cinnabarinus for a long period of time.

The invention discloses a spider mite preventing and treating compound leaf fertilizer and a preparation method thereof. The spider mite preventing and treating compound leaf fertilizer comprises the following components in percentage by weight: 8% of geranium extract liquor, 30% of potato stem leaf extract liquor, 25% of beet root extract liquor, 15% of wormwood extract liquor, 1% of boric acid and 21% of emulsifier, wherein the components are processed and prepared into emulsion. During the use, water is added to the spider mite preventing and treating compound leaf fertilizer to obtain liquid which is 500-1000 times of the original weight to carry out spraying; tests prove that the mixture which is 500 times of the original weight has the remarkable effect in the frequently occurred two-spotted spider mites and purple spider mites, and after continuously spraying the mixture for 7 days, the spider mites become colloids; every 7 days, the 1000-times liquid is used for preventing spider mite; in the frequently occurred areas, no spider mites invade to the sprayed areas; and moreover, the spider mite preventing and treating compound leaf fertilizer has trace elements required by the growth of the plants, has a product for promoting the photosynthesis, and has elements for forming carbohydrate so as to promote blossoming and fruit bearing. The spider mite preventing and treating compound leaf fertilizer is safe, environment-friendly and non-toxic, has trace element fertilizer and has the function of preventing and treating spider mites.

The invention belongs to the technical field of compounding pesticides, and specifically relates to missible oil containing flonicamid and bifenthrin and an application of the missible oil. The effective components of the missible oil are flonicamid and bifenthrin in a weight ratio of 20:1-1:1. The missible oil containing flonicamid and bifenthrin is particularly beneficial to prevention and treatment of tetranychus telarius linne by virtue of synergistic interaction effects of the components.

The invention discloses a cytochrome P450, the amino acid sequence of which is shown in SEQ ID NO: 2. The P450 gene is overexpressed in the populations with low to medium level resistance to organic phosphorus in the field. The gene can be used as a molecular Marker-assisted early detection and early warning of resistance to organophosphorus insecticides in the field Tetranychus urticae population, and the detection process is simple and fast. The gene can metabolize chlorpyrifos and pirimiphos-methyl in vitro, and is expected to metabolize other organophosphorus pesticides. Therefore, the engineered bacteria expressed by this gene can degrade organic phosphorus pesticide residues in fruits and vegetables, waste water and sewage It has the potential for industrial development and application. The cloned P450 gene involved in drug resistance can also be used as a target to design inhibitors of specific detoxification enzymes and provide ideas for the development of new drugs in the future.

Provided are a lycoris radiata bulb extractive pesticide and a method for preparing an extractive. Lycoris radiata bulbs can be manufactured into the lycoris radiata bulb extractive pesticide in forms of missible oil, microemulsion, wettable powder and the like through certain processing procedures. The lycoris radiata bulb extractive pesticide has good insecticidal ovicidal activities to tetranychus, plutella xylostella, vegetable aphides, armyworms and pine wood nematodes and protective effect in fields and woodlands. The pesticide is remarkable in effect, low in toxicity and residuals, simple in preparing process, low in cost and suitable for popularization and application and accords with the development tendency of existing pesticides, and pests do not easily produce drug resistance.

The invention provides a method for propagating Oriusminutus artificially, which comprises the following steps: cultivation of spawning substrate, feeding of host; preparation of instruments of production, preparation of artificial feedstuff and preparation of seeds are carried out; under the conditions of 25 DEG C, 75% of PH and 16L:8D of illumination, kidney bean buds are taken as the spawning substrate, and Tetranychus cinnabarinus or the artificial feedstuff is used for feeding; firstly, the kidney bean buds on which the Oriusminutus spawn is produced are placed in a large tray, and kidney bean seedlings with proper Tetranychus cinnabarinus are placed in the large tray by interpenetration, according to the age period of Oriusminutus, the number of the proper Tetranychus cinnabarinus is confirmed; then the Oriusminutus with the tray are placed in an insect breeding cage for feeding the Oriusminutus. Spawn needs 12-16 days to grow to be imago, and the production period is about 30 days. The method is applicable to feeding with large amount and being promoted, thus laying the solid foundation for producing Oriusminutus with large amount and providing great convenience to biological control.

The invention belongs to the technical field of agricultural microbiology, and particularly relates to a protein BVP10 for preventing and treating spider mites and application of the protein BVP10. The protein is shown as SEQ ID NO.2. The medial lethal concentration of the BVP10 protein to tetranychus urticae is 19.07 mug / mL, the medial lethal concentration of the BVP10 protein to panonychus citri is 58.05 mug / mL, the medial lethal concentration of the BVP10 protein to tetranychus cinnabarinus is 36.08 mug / mL, the prevention and treatment effect on strawberry red spider mites is 79.53 percent to 95.45 percent, and the protein provides new selectivity for preparation of novel acaricides.

The invention belongs to the technical field of agricultural microbiology, and specifically relates to a protein BVP10 for preventing and controlling spider mites and its application, and the protein is shown in SEQ ID NO.2. The half-lethal concentration of BVP10 protein against Tetranychus urticae was 19.07 μg / mL, the half-lethal concentration against Panonychus citrus was 58.05 μg / mL, the half-lethal concentration against Tetranychus cinnabarinus was 36.08 μg / mL, and the half-lethal concentration against spider mite The control effect is 79.53%~95.45%. The protein provides a new selectivity for the preparation of new acaricides.

The invention discloses a breed conservation breeding method for Tetranychus cinnabrinus. The method comprises the steps that a box with a box cover is taken, a sponge with the section slightly smaller than a box body is arranged at the bottom inside the box in a cushioned mode, a layer of black plastic film with the area slightly smaller than the sponge is laid on the sponge, a proper amount of water is added, a moisture cotton sliver wraps the black plastic film, a half of the cotton sliver is arranged on the plastic film, and the other half of the cotton sliver is arranged on the sponge; cleaned and fresh erythrina crista-galli leaves are wiped dry, leaf stalk is wrapped by the moisture cotton, the back sides of the leaves face upwards, the leaf stalk is pressed by the cotton sliver, a plurality of fresh erythrina crista-galli leaves are placed, Tetranychus cinnabrinus is picked up to be placed on the leaves, the box cover covers the box reversely, and a gap is formed in the length direction of the box in a staggered mode; according to the breeding condition, the temperature is 25-27 DEG C, the relative humidity is 60-70%, the photoperiod is 14L:10D, and the leaves are replaced with fresh ones every two days. The population size of the Tetranychus cinnabrinus can be effectively controlled, and the population can be stored for a long time, so that the Tetranychus cinnabrinus sensitivity or high resistance strain is obtained.