Bursaphelenchus xylophilus effector BxSCD1 interaction protein as well as coding gene and application thereof

Abstract

The invention discloses a pine wood nematode effector BxSCD1 interaction protein as well as a coding gene and application thereof, and belongs to the technical field of molecular biology. The pine wood nematode effector BxSCD1 interaction protein has an amino acid sequence as shown in SEQ ID No. 1, and a nucleotide sequence for expressing the protein is as shown in SEQ ID No. 2. Starting from an effector secreted by the pine wood nematode and a host target, the interaction between a host pine tree and the pine wood nematode is studied, it is found that BxSCD1 silencing can delay the disease attack of the black pine, and BxSCD1 inhibits expression of genes related to the disease course of the black pine; through interaction of the BxSCD1 and the pine tree ethylene synthase gene ACO1, it is proved that the BxSCD1 can influence the immune response of the pine tree by influencing the expression quantity of the ethylene synthase ACO1 of the black pine, and the BxSCD1 has important scientific significance in the aspects of revealing the pathogenesis of the pine wood nematode, early detection of the occurrence of the pine wood nematode disease and the like.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and specifically relates to BxSCD1 interaction protein of pine xylophilus effector, its coding gene and application. Background technique [0002] Pine wood nematode disease, also known as pine wilt disease (PWD), is a major forest disease that has seriously damaged and threatened the ecological environment and forest resources in my country and the world. Since pine wood nematode was first discovered in Nanjing Zhongshan Mausoleum in 1982, 18 provinces (autonomous regions and municipalities directly under the Central Government) including Jiangsu, Zhejiang, Shandong, Anhui, Fujian, Guangxi, Liaoning, Tianjin, Sichuan and Hubei have become pine wood nematode epidemics. Area. After being infected by pine xylophilus, the needle leaves turn yellowish brown or reddish brown, and some branches die until the whole plant wilts. The pathogenic mechanism of pine wood nematode disease has not y...

AI Technical Summary

Problems solved by technology

As a migratory plant-parasitic nematode, pine xylophilus has not been reported on its host targets for secreting effectors and a series of immune responses during the infection process

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