38 results about "Xylophilus" patented technology

The invention provides a pine xylophilus RNAi regulation gene, the full-length sequence of the RNAi regulation gene is shown in SEQ ID NO:1, and the full-length cDNA sequence of the RNAi regulation gene is shown in SEQ ID NO:2. The present invention also provides the application of the pine wood nematode RNAi regulation gene in the preparation of pine wood nematode control bacteria agent. The present invention screens out a highly efficient RNAi regulation gene segment, and feeds the positive fungal transformants containing the gene segment to pine wood nematodes to obtain an obvious RNAi phenotype, the gene expression of pine wood nematode Bx-etr-1 is significantly down-regulated, and the pine wood nematode The nematodes have abnormal embryonic development, high larval mortality, changed trajectory, slowed movement speed, and significantly decreased population.

The invention relates to the application of punicalagin in killing of pine wood nematodes; the concentration of the punicalagin is 200-1000micro-g / mL, the punicalagin is capable of effectively killing the pine wood nematodes, the punicalagin has the median lethal concentration of 332.88micro-g / mL within 72 hours for the pine wood nematodes; the punicalagin is capable of effectively inhibiting the activity of acetylcholine esterase of the pine wood nematodes so as to kill the pine wood nematodes; the punicalagin has the half inhibiting concentration of 654.92micro-g / mL. The punicalagin is obtained by being separated from pomegranate bark. The punicalagin is simple in operation method, wide in raw material source, low in cost, high in efficiency and low in toxicity, safe and environmentally friendly; the punicalagin has a good application prospect in the aspect of controlling pine wilt diseases, thus being applied to pesticide preparations for killing the pine wood nematodes.

The invention discloses polypeptides and nucleic acids that mediate the endocytosis of exogenous proteins and their application, and a method for gene editing in Neocaridina heteropoda. The invention finds a length of A polypeptide of only 40aa, the polypeptide has an unexpected effect of mediating the endocytosis of exogenous proteins, so it is easy to express, purify, form RNP complexes and be endocytosed, and has a good effect in the gene editing of Neopodia prawn Application prospects. This technology can be widely applied to non-model organisms that are not yet proficient in embryonic microinjection technology, and is of great significance for Cas9-mediated gene editing.

The invention relates to the technical field of nematode disease prevention and control, in particular to the mog-2 gene of pine xylophilus, and a patent application for using the gene to interfere with the development of pine xylophilus to prevent and control pine xylophilus disease. The full-length DNA sequence of mog‑2 gene of pine wood nematode is 953bp, including 3 introns and 4 exons, and the start codon and stop codon of the DNA sequence are ATG and TGA, respectively. The length of CDS sequence of mog-2 gene of B. xylophilus composed of exons is 843bp. This application has cloned the mog‑2 gene of pine wood nematode and analyzed its function. It is believed that the gene is closely related to the growth and development of pine wood nematode, especially sex determination; using siRNA to interfere with the development of pine wood nematode can cause pine wood nematode Nematodes are obviously masculinized and their population fecundity is reduced. The siRNA can be used to interfere with development to prevent and control pine wood nematode disease.

The invention belongs to the technical field of agricultural pest control, and in particular relates to a biological control method for effectively controlling pine wood nematode disease by biological control of monochamus. The method for effectively controlling pine wood nematode disease by the biological control of Monochamus alternata described in the present invention is to apply Cornus officinalis extract in pine trees containing Monochamus larvae before the pupation of Monochamus alternata at the end of April and the beginning of May each year The method of / morroniside can effectively block the pupation of monochamus alternatus, stop the development of monochamus larvae, reduce the population density of pine xylophilus pupae and adults, and then reduce the mortality of pine trees. At the same time, it also effectively prevents Subsequent pine wood nematodes formed a diffuse fourth-instar nematode with the eclosion of Monochamus alternatus, which effectively blocked the spread of pine wood nematodes, thereby reducing the mortality rate and spread speed of pine trees in areas where pine wood nematodes occurred, and preventing the spread of pine wood nematodes in forests. The prevention and control goal of further spread and expansion in the region can solve the problem of large-scale reproduction and rapid spread of pine wood nematode in the place where the pine wood nematode epidemic occurs.

The invention discloses a high-synchronization bursaphelenchus xylophilus resistant pinus densiflora somatic embryogenesis and plant regeneration method. The high-synchronization bursaphelenchus xylophilus resistant pinus densiflora somatic embryogenesis and plant regeneration method comprises the steps of inducement and proliferation of embryonic callus as well as maturation, germination and transformation of somatic embryos; and the maturation culture of the somatic embryos is carried out by using a liquid-solid proliferation-solid maturation method. According to the high-synchronization bursaphelenchus xylophilus resistant pinus densiflora somatic embryogenesis and plant regeneration method, with immature zygotic embryos of bursaphelenchus xylophilus resistant pinus densiflora as explants, mature somatic embryos and regenerated plants are successfully obtained by carrying out tests, such as somatic embryo maturation, germination and plant regeneration, on the embryonic callus obtained by successfully inducing the immature zygotic embryos of bursaphelenchus xylophilus resistant pinus densiflora; the mature somatic embryos and the regenerated plants are transplanted and survive; and the germination rate and plant transformation rate of the somatic embryos generated on a maturation culture medium are high and are respectively 67.2% and 46.5%; therefore, the high-synchronization bursaphelenchus xylophilus resistant pinus densiflora somatic embryogenesis and plant regeneration method provides an important scientific basis and technical support for large-scale propagation and factory production of the bursaphelenchus xylophilus resistant pinus densiflora.

The invention discloses a chitosan nanoparticle which is formed by raw materials in percentage by weight: 0.5-5% of chitosan, 1-10% of a cosolvent, 0-5% of an anti-freezing agent, 0.1-1% of a surfactant, 0.1-0.5% of an emulsifying agent, 40-60% of buffering liquid and of the balance of water. The invention further provides a biological pesticide preparation and a preparation method thereof. The biological pesticide preparation is mainly prepared from the following components in percentage by weight: (A) 0.05-15% of an effective component, (B) 19.95-55% of a solvent, and (C) 30-80% of the chitosan nanoparticle, wherein in the effective component, siRNA (small interfering Ribonucleic Acid) is synthesized aiming at pine wood nematode efficient lethal target genes and the siRNA is 15-50nt double-chain RNA. According to the chitosan nanoparticle, the stability of the biological pesticide preparation is better and the effect of preventing and treating pine wood nematodes is better.

The invention relates to a paecilomyces metabolite and application thereof, which belong to the technical field of microbial medicine screening. The invention is realized by the following steps that: a production strain is paecilomyces sp.1788, and the preservation code is CGMCCNo. 3422; the paecilomyces sp.1788 is fermented and cultured by czapek culture medium conventional liquid, the fermentation fluid is concentrated at low pressure, column chromatography is carried out to water phase repeatedly, and the paecilomyces metabolite of the invention is prepared after elution. The paecilomyces metabolite of the invention has the function of killing bursaphelenchuh xylophilus, the lethal rate (ED50) in 24h is 299.5ppm, and the ED95 is 796.2ppm, and can be applied to bursaphelenchuh xylophilus preparations. Compared with the traditional bursaphelenchuh xylophilus chemical, the invention is characterized by high efficiency, low toxin, water solubility, easy preparation and the like.

The invention discloses a gRNA for detecting pine xylophilus, comprising a) a guide sequence such as SEQ ID NO: 1, which can hybridize to a target nucleotide sequence, and b) a framework nucleic acid fragment interacting with Cas nuclease . The invention also discloses a kit for detecting pine xylophilus, which comprises the gRNA or the DNA that can be transcribed into the gRNA. The present invention also discloses a vector system, which comprises one or more vectors, the vector comprising: a first regulatory element and a second regulatory element, the first regulatory element is operably linked to a nucleoside encoding Cas nuclease An acid fragment, the second regulatory element is operably linked to the nucleotide fragment encoding the gRNA.

The invention relates to an active material extracted from euphorbia lathyris and application thereof, and belongs to the technical field of biological pesticides. The active material extracted from euphorbia lathyris is characterized in that a method for obtaining the active material comprises the following steps of: a, cleaning collected branches or leaves of the euphorbia lathyris by tap water, putting the cleaned branches or leaves in an oven for roasting at the temperature of 50 DEG C for 10 hours, cutting 10g of dried branches or leaves into pieces, and putting the cut branches or leaves in a triangular flask, soaking the branches or leaves by 80 percent ethanol, wherein the time for the first soaking extraction is 4 hours, and the time for the second and the third soaking extractions is 12 hours and 24 hours respectively; b, mixing extraction solution obtained by the extractions at the third time, condensing the obtained solution until the solvent is vaporized to obtain coarse extracts of the branches or leaves, dissolving the coarse extracts in methanol to prepare the solution of which the concentration is 0.1g / ml, and putting the obtained solution in a refrigerator for later use. The active material can be used for preparing a biological reagent of killing B.xylophilus. The active material has the advantages of low cost, simple and convenient preparation method, high efficiency and low toxicity, which can be used for preparing the biological reagent of killing B.xylophilus.

The invention relates to a biological pesticide capable of preventing and treating bursaphelenchus xylophilus and a preparation method thereof as well as application of the biological pesticide to prevention and treatment of the bursaphelenchus xylophilus. A dosage form of the pesticide is powder; an active component of the powder is Trichoderma virens T43 fermented liquid n-butanol extract; the preparation method of the powder comprises the following steps: filtering and centrifuging fermented liquid, taking supernatant liquid, adding n-butanol, mixing and extracting, removing a water layer,and evaporating and concentrating an n-butanol layer to obtain the powder.

The invention provides a mobile terminal-based integrated early warning and detection method and device for pine wood nematode disease. The mobile terminal-based integrated early warning and detection method for pine wood nematode includes: extracting features from first image information, and then performing feature extraction on first image information. When the image information includes the characteristics of discolored wood, the characteristics of the host species of B. xylophilus, and the characteristics of the B. xylophilus vector, obtain the second image information corresponding to the target stand; perform feature extraction on the second image information, where the second image information includes In the case of pine wood nematode characteristics, the damage stage detection information of the target diseased trees in the target stand is generated based on the first image information; wherein the first image information includes biological information of at least a part of the area in the target stand, and the second image information includes The wood segment information of the target discolored wood in the target stand. The mobile terminal-based integrated early warning and detection method for pine wood nematode disease of the present invention can realize rapid, efficient and accurate pre-disaster early warning and post-disaster detection of pine wood nematode.

The invention relates to a class of compounds with the activity of poisoning and killing pine xylophilus and applications thereof, belonging to the technical field of biological pesticides. Such active compounds are obtained from production strains by conventional solid fermentation and extraction and separation methods. Its production strain is Gliocladium roseum (Gliocladium roseum) Gr87, which has been preserved in the China Committee for the Preservation of Microorganisms on October 9, 2002, with a preservation number of CGMCC No.0807; this type of active compound consists of 6 compounds The composition can effectively poison and kill pine xylophilus, and can be used alone or in combination to prepare nematicidal biopesticides.

The invention discloses a fungi Xylaria sp.FDYS-1, biological agents prepared by the fungi Xylaria sp.FDYS-1 and application of the fungi Xylaria sp.FDYS-1 in prevention and cure of pine wood nematodes. The fungi Xylaria sp.FDYS-1 is preserved in China Center for Type Culture Collection on February 25th, 2008 and the preservation No. is CCTCC NO:M 208025. The invention inoculates the Xylaria sp.FDYS-1 into a potato glucose liquid medium or a potato sugar liquid medium; the pH of the liquid medium is 5 to 8; the culture temperature is 26 DEG C to 29 DEG C; after cultivation of 3 to 5 days under nonluminous conditions, the culture medium is centrifugally filtered, and the biological agents are obtained by collection of filtrate. The biological agents have high killing activity on the pine wood nematodes, good thermal stability, environment-friendly source without toxity, strong selectivity and small ecological environmental influence. The fungi Xylaria sp.FDYS-1 has low culture condition requirements, basically maintains stable inheritance of the capacity for generation of active materials, and has good development and application prospect.

The invention discloses protein BxCDP1 of bursaphelenchus-xylophilus pathogen-associated molecular patterns and application thereof. The amino acid sequence of the protein BxCDP1 of the pathogen-associated molecular patterns (PAMPS) is shown in SEQID NO.2. From effectors secreted by bursaphelenchus xylophilus and the PAMP, the defensive responses of a host plant, namely, a pine tree, to bursaphelenchus xylophilus invasion are studied, the protein BxCDP1 of the pathogen-associated molecular patterns is obtained from the bursaphelenchus xylophilus, it is proven through experiments that the protein BxCDP1 can trigger cell necrosis of various plants including the host plant, has a certain broad spectrum in cell necrosis triggering, and stimulates the defensive response of the host plant. The BxCDP1 triggered cell necrosis depends on a co-receptor BAK1 of pattern recognition receptors, the BxCDP1 can stimulate the accumulation of nicotiana benthamiana ROS and up regulation of PTI Marker genes, and the immunoreaction of the nicotiana benthamiana is stimulated. It can be seen that the BxCDP1 is a PAMP secreted by the bursaphelenchus xylophilus, and has important theoretical and practicalsignificance for revealing the pathogenic mechanism of the bursaphelenchus xylophilus and improving the resistance of the pine tree to the bursaphelenchus xylophilus in a targeted mode.