Trehalase gene Bx-tre1 and application thereof
A technology of trehalase and gene, which is applied in the field of trehalase gene Bx-tre1, can solve problems such as the spread of pine wood nematodes, achieve the goal of inhibiting sexual reproduction, reducing the activity of trehalase, and blocking the metabolic pathway of trehalose Effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
specific Embodiment approach 1
[0012] Specific embodiment 1: The nucleotide sequence of a trehalase gene Bx-tre1 in this embodiment is shown in the sequence table Seq ID No: 1, with a length of 1413 bp.
[0013] The expression level of the trehalase gene in this embodiment is significantly higher in the male pine xylophilus than in other stages, indicating that the gene plays an important role in the male pine xylophilus. Reducing the expression of this gene in male pine wood nematodes by gene silencing can effectively reduce the enzyme activity of trehalase and block the metabolic pathway of trehalose, thereby reducing the 55%-60 % survival rate, thereby inhibiting sexual reproduction, has important application value in the control of pine wood nematode.
specific Embodiment approach 2
[0014] Specific embodiment two: the difference between this embodiment and specific embodiment one is: the nucleotide sequence of the upstream primer for constructing the trehalase gene Bx-tre1 is shown in the sequence table Seq ID No: 2; the nucleotide sequence of the downstream primer The sequence is shown as Seq ID No:3 in the sequence table. Others are the same as the first embodiment.
specific Embodiment approach 3
[0015] Embodiment 3: In this embodiment, the trehalase gene Bx-tre1 is applied to the prevention and treatment of pine diseases.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com