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265 results about "Pinewood nematode" patented technology
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Jump to navigation Jump to search. Bursaphelenchus xylophilus, commonly known as pine wood nematode or pine wilt nematode (PWN), is a species of nematode that infects pine trees and causes the disease pine wilt. It occurs in much of the United States, Canada, and Mexico.
Technology for artificial domestication and breeding of sclerodermus and applied to prevention and treatment of pine sawyer beetles
The invention discloses a technology for artificial domestication and breeding of sclerodermus and applied to prevention and treatment of pine sawyer beetles. The technology for artificial domestication and breeding of sclerodermus comprises the following steps of: firstly, feeding the sclerodermus to be substituted for the host of a barley pest; secondly, carrying out the selecting and refrigerating treatment of female sclerodermus; thirdly, packing the initiated pupa barley pest and the female sclerodermus into a finger-shaped tube, and plugging the tube with an ordered pine plug; and finally, neatly stacking and placing the tubes into a weather cultivation box with a cultivation temperature and conditions as well as prompts. In a bursaphelench xylophilus epidemic area, the throwing technology of sclerodermus comprises the following steps of: firstly, setting bait woods to induce the pine sawyer beetles to lay eggs; then examining the parasitic situation of the pine sawyer beetles in a pine tree section in an appropriate weather condition, and releasing the sclerodermus according to the groove number of the sawyer beetles; and finally investigating the parasitic situation of the sclerodermus. By the adoption of sawyer pests to prevent and treat the pine sawyer beetles so as to inhibit the occurrence of a bursaphelench xylophilus disease, the environment can not be polluted, the ecological balance is maintained, the biological diversity is kept, and the technology is an advanced technology for controlling the pine sawyer beetles and the bursaphelench xylophilus disease.
Owner:浙江森得保生物制品有限公司 +2
Monochamus alternatus adult attractant
ActiveCN102986739AImprove trapping effectSuppress and delay the spread of transmissionBiocidePest attractantsAlcoholTrapping
The invention provides a Monochamus alternatus adult attractant. The Monochamus alternatus adult attractant comprises an artificially-synthesized compound, and the compound is prepared through adding reagents comprising undecyl alcohol, thionyl chloride, glycol, metallic sodium and the like. The Monochamus alternatus adult attractant comprises the artificially-synthesized compound, so the effective application amount is less; the Monochamus alternatus adult attractant has a high trapping effect on Monochamus alternatus, and can be used as an effective means for monitoring and controlling the Monochamus alternatus to inhibit and delay the propagation of the pine wilt disease; and the Monochamus alternatus adult attractant generally has the advantages of simple components, low production cost, safety, environmental protection, high insect trapping efficiency, and advantage obviousness.
Owner:福建辰康农林科技有限公司
Captive-breed piebald dastarcus helophoroides and technology applied to prevent and control monochamus alternatus hope
The invention discloses a captive-breed piebald dastarcus helophoroides and a technology applied to prevent and control monochamus alternatus hope; the technology has the following steps: first, feeding imagoes of the piebald dastarcus helophoroides in the environment with the environmental temperature ranging from 23 DEG C to 27 DEG C and humidity ranging from 55 DEG C to 65 DEG C and inducing the imagoes to oviposit in large quantity and incubate larvae of the piebald dastarcus helophoroides; then using an alterative host to facilitate the larvae to live a parasitic life and grow, thus finally eclose to imagoes; then preparing the ovum or imagoes of the piebald dastarcus helophoroides into ovum release cards or imago release bags or finger-shaped tubes to be released in forest stands endangered by monochamus alternatus hope in the bursaphelenchus xylophilus disease area. With natural enemy insects adopted to control pests, the pests and the disease can be brought under effective andsustained control in the premise of not polluting the environment, maintaining ecological balance and biodiversity, therefore, the technology of the invention is an advanced technology for controlling monochamus alternatus hope and bursaphelenchus xylophilus disease.
Owner:INST OF FOREST ECOLOGY ENVIRONMENT & PROTECTION CHINESE ACAD OF FORESTRY
Pinewood nematoda detection reagent-box and detection method
InactiveCN1529164AImprove practicalityImprove detection efficiencyMicrobiological testing/measurementWood testingNematodePinewood nematode
Two pairs of primer are designed: Pl / P3 is an idiocratic primer for pine wood nematode; P2 / P3 an idiocratic primer for quasi pine wood nematode. The kit includes 1.25ml solution I,1ml solution II, 100U tag enzyme, and 50ul for each contrapositive DNA of wireworm. The solution I is as following constitutes: Worm Lysis Buffer, 500mM KCl, 100mMTris-ClpH 8.0, 15mM MgCl2, 10mMDTT, 4.5% Tween20, 0.1%gelatin, 0.2ug / ul protease K. The solution II is as following constitutes: 2xPCR reaction Buffer, 4.0mM MgCl2, 0.2mM dNTP, 0.8 mu MP1, P2, P3. The invented kit possesses high specificity, sensitiveness and stability, providing fast, sensitive and specific technical method.
Owner:NANJING AGRICULTURAL UNIVERSITY
Method for preparing bacteria-free pine wood nematodes
The invention discloses a method for preparing bacteria-free pine wood nematodes. The method for preparing the bacteria-free pine wood nematodes comprises the following steps: culturing the pine wood nematodes; preparing pure pine wood nematode eggs; soaking the pine wood nematode eggs in 15% H2O2 for 50-60 minutes; incubating the pine wood nematodes; and culturing the pine wood nematodes and performing bacteria-free identification, thereby preparing the bacteria-free pine wood nematodes. As for the method for preparing the bacteria-free pine wood nematodes, the property of being capable of being adhered on a glass vessel of the pine wood nematode eggs is utilized, a small culture dish is adopted for collecting the eggs, the newly prepared 15% H2O2 is further used for performing sterilization treatment, the bacteria-free pine wood nematode eggs are obtained, the bacteria-free eggs are incubated, mass propagation is further performed on botrytis cinerea, and then the bacteria-free pine wood nematodes are finally obtained. Under sterile conditions, the obtained bacteria-free pine wood nematodes are used for performing an inoculation test on seedlings cultured by spruce bacteria-free tissues, and the result shows that the inoculation of the bacteria-free pine wood nematodes can still enable the bacterial-free seedlings to wilt. The method can be adopted for enabling the pine wood nematodes to be bacteria-free and lose pathogenicity, thereby being the simple and successful method.
Owner:NANJING FORESTRY UNIV
Method for preparing extractive of perilla for killing activity of pine wood nematode
InactiveCN1795724AEnhance killing activitySimple manufacturing methodBiocideAnimal repellantsOrganic solventAdditive ingredient
A process for preparing the extract of perilla with the activity to kill the pine nematode includes such steps as providing fresh perilla leaves, immersing in distilled water, vacuum concentrating, and extracting in organic solvent. Its advantages are high killing activity and no poison.
Owner:QINGDAO UNIV
High-spectrum comprehensive analysis early detection method for Bursaphelenchus xylophilus
InactiveCN101776584AImprove detection accuracyMeasuring parameters are simple and intuitiveScattering properties measurementsColor/spectral properties measurementsRed edgeRadiometer
The invention relates to a high-spectrum comprehensive analysis early detection method for Bursaphelenchus xylophilus. In the outbreak season of the Bursaphelenchus xylophilus, a pine needle sample is picked up at regular intervals and sealed by a freshness protection package, then spectrum data of the sample is determined by a field spectrum radiometer under an artificial light source in a darkroom; and a red edge, a green peak reflection height, a red light absorption depth, a red-green index, a light green index and a fractal dimension are calculated, and then the values are comprehensively analyzed to obtain a detection result. The method has the advantages that: the spectrum characteristics of the pine changed by a disease are detected from the fine characteristic to the integral characteristic through the approximately continuous high-spectrum data of visible light, near-infrared light and part of intermediate infrared light, and microscopy and macroscopy are integrated; and the various characteristic indexes are comprehensively applied, and the relationships among the characteristics are considered adequately, wherein the red-green index and the light green index are promoted for the first time in the invention, and reflect the photosynthesis and the water content of plants respectively.
Owner:ZHEJIANG FORESTRY UNIVERSITY
Injection containing emamectin benzoate and application of injection
InactiveCN110100831AStable structureHas a bactericidal effectBiocidePlant growth regulatorsDiseaseAlcohol
The invention discloses an injection containing emamectin benzoate and application of the injection. The injection is prepared from the emamectin benzoate, chitosan oligosaccharide, an organic solventand a surface active agent, wherein the weight ratio of the emamectin benzoate to the chitosan oligosaccharide is 20:(1-100); the emamectin benzoate accounts for 0.1wt%-10wt% of the injection; the deacetylation degree of the chitosan oligosaccharide is equal to or higher than 50%; the organic solvent is lower alcohol which contains 1 to 8 carbon atom chains; the organic solvent accounts for 0.1wt% to 95wt% of the injection. The injection is suitable for trunk injection, various pests of woods are prevented, and the injection has the advantages of being low in cost, high in prevention efficiency and wide is application range. The injection is suitable for prevention and control over pests and diseases of the woods, and especially for prevention and control over monochamus alternatus and other wood borers of pine trees, and prevention and control over pine wood nematode, and therefore, the pine wood nematode disease is controlled and prevented.
Owner:谢祥英
Culture of nematoda without bacteria
An aseptic culture method for pine nematode includes such steps as culturing Pseudomonas fluorescens GcM5-1A (CCTCC No.M204065), deactivating, culturing the calli of black pine, deactivating, preparing the aseptic ova of pine nematode, preparing the culture liquid from deactivated cells and calli and aseptic water, adding said aseptic ova, and culturing at 15-40 deg.C for 4-20 days.
Owner:赵昕
Method for early diagnosis and detection of pine nematode disease
InactiveCN1420180AConducive to early censusFree from peelingBiocideMicrobiological testing/measurementMicroscopic observationNematode
A process for earlierly detecting pine nematosis includes inoculating the disc fungus (Ps0202) or other microbial fungi on culture medium, drilling circular hole on the pine trunk to be detected, inserting the test tube containing said fungi in said hole, staying it in the hole for 3-5 days, and determining by microscope. Its advantages are easy operation and high sensitivity.
Owner:XIAMEN UNIV
A pine wilt disease tree identification and positioning method and system
ActiveCN109766744AImprove recognition rateHigh positioning accuracyImage analysisCharacter and pattern recognitionDiseaseNematode
Owner:BEIJING RES CENT OF INTELLIGENT EQUIP FOR AGRI
Method for efficiently inducing generation of adventitious roots of Pinus densiflora tissue culture plantlets
ActiveCN101589690AQuality improvementLess callusPlant tissue cultureHorticulture methodsDiseaseSucrose
The invention discloses a method for efficiently inducing the generation of adventitious roots of Pinus densiflora tissue culture plantlets, which uses growing young Pinus densiflora tissue culture plantlets as explants, and adopts a 'two-step method', namely firstly, inducing the generation of adventitious root blastemas in a culture medium containing 1 / 2 to 1 / 4 of WPM, 0.1 to 0.2 mg / L NAA, 1.0 to 2.0 mg / L IBA and 0.5 to 1.5 percent sucrose, and secondly, transferring the explants into a culture medium containing 1 / 2 of the WPM, 0.5 to 1.0 g / L AC and 2.0 percent of the sucrose to promote the expression and the extension of the adventitious roots so as to finish the generation process of the adventitious roots. By adopting the method, the generation ratio of the adventitious roots of the Pinus densiflora tissue culture plantlets is high, the root system develops well and the transplant survival rate is high, thus the method lays a solid foundation for the mass production of Pinus densiflora tissue culture regeneration plants for resisting pine wood nematode diseases, and simultaneously has important reference value for the cultivation of other pine species tissue culture plantlets.
Owner:NANJING FORESTRY UNIV
Wireworm-killing biologic bacterial agent, its preparation and application
The present invention relates to a process for preparing and application of bionematocide and belongs to technique domain of biological pesticide. Product of the invention can be gained from conventional method that the produced strain is cultivated in test tube, enlarged cultivated in liquid and extracts active constituents of nematocide, whose produced strain is Lecanicillium psalliotae YMF1.00112, with conservation number CGMCC No.1312. The formulation of enlarged liquid base in the invention is, dextrose 10g, ammonia sulfate 1g, magnesium sulfate 1g, ferrous sulfate 0.015g, leachate of potato 1000mg, pH6.5. the invention can poison pine wood nematode and Panagrellus redivivus and be used as bio-prevention to plant parasitic nematode. The product has the advantage of high poison effect, safety to human body and environment protection, etc.
Owner:YUNNAN UNIV
Method for effectively controlling bursaphelenchus xylophilus by biologically controlling monochamus alternatus
InactiveCN104871886AIncreased parasitic abilityAnimal huntingPlant protectionBombus hypocritaZoology
The invention discloses a method by the adoption of biological control. According to the method, the populations of the monochamus alternatus of pine forests are obviously reduced and controlled, and the number of pines which die from bursaphelenchus xylophilus is also obviously reduced. Drone pupae of bombus hypocrita are adopted as hosts of sclerodermus sp., meanwhile proliferation conditions are regulated and controlled, and therefore the parasitic ability of the sclerodermus sp. obtained through proliferation is remarkably improved.
Owner:INST OF FOREST ECOLOGY ENVIRONMENT & PROTECTION CHINESE ACAD OF FORESTRY
Kit for detecting pine beam nematode and detecting process thereof
ActiveCN101338342AAccurate discriminationQuick DiscriminationMicrobiological testing/measurementFluorescence/phosphorescenceForward primerFluorescence
The invention discloses a pine wood nematode detecting reagent box which takes 5'ACC ATT CGG TTG GCT CTG TT 3' as a forward primer (F), takes a 5' CCC TAA GGC GTC GGT GAA C 3' as a reverse primer (R) and takes 5' FAM- TAG CTG AGC ATC TTT T-TAMRA 3' as a fluorescent probe (P). The invention also discloses a method for adopting the reagent box to detect the pine wood nematode. The primers and the probe in the pine wood nematode detecting reagent box of the invention have higher specificity, sensitivity as well as stability and provides a fast, sensitive and specific method for detecting the pine wood nematode.
Owner:NANJING FORESTRY UNIV
Fast detection method of pine tree wilt disease pathogenic nematode
InactiveCN1793866AShort manufacturing timeLess prone to lossMaterial analysis by observing effect on chemical indicatorMicrobiological testing/measurementNematodePinewood nematode
A method for quickly detecting pathogenic threadworm of pine wilt disease includes designing each pair of specific primer for PCR detection of pine threadworm and pine imitate-threadworm, obtaining single threadworm DNA as template by mechanical grinding and filtering paper absorption, carrying out specific PCR amplification of pine threadworm and pine imitate-threadworm as well as detecting PCR product.
Owner:YUNNAN AGRICULTURAL UNIVERSITY
Primer and method for detecting specificity of pine wood nematode in Chinese red pine died wood of illness, and application thereof
InactiveCN101586160ARealize detectionQuick checkMicrobiological testing/measurementDNA/RNA fragmentationRed pineNematode
The present invention discloses a primer and a method for detecting a specificity of a pine wood nematode in a Chinese red pine died wood of an illness, and an application thereof, wherein a nucleotide sequence of an upstream primer of the specificity detection primer is represented by SEQID NO:1, a nucleotide sequence of a downstream primer is represented as SEQ ID NO:2. the primer of the invention is capable of separating the pine wood nematode from other 12 nematodes of the Chinese red pine fat wood and Chinese red pine as well as common four fungus and ten bacteria of the Chinese red pine. the invention also provides a method for implementing a PCR amplification of the pine wood nematode of the diseased wood directly without separation of the pine wood nematode from the diseased wood, after rubbing the diseased wood liquid nitrogen, by means of two cleavages of WLB and protease K, a DNA formwork for the PCR amplification will be obtained after purifying, the amplification efficiency will achieve 100%, a separation time is saved and a detection efficiency is improved. The detection method of the invention is simple and high-efficiency, has a high accuracy and the specificity; it is capable of being produced into reagent kits to popularize widely.
Owner:SOUTH CHINA AGRI UNIV
Bacterial agent capable of killing nematode and application thereof
The invention relates to nematocide and its appliances thereof, belonging to the field of biological pesticide technique. Said nematocide is acquired through the sequence of culturing preparing strain in test tube, fluid enlargement culture and extracting nematocide active component, and said preparing strain is as follows: germinated barley-acceptable single cell bacteria stenotrophomonas maltophilia G6 which is preserved in microbe bacterial preservation management commission common microbe center of China with preservation data: 2005-10-31 and preservation registration number CGMCC No: 1522. The main mechanism of action of G6 strain is to break up body wall of worm through producing enzyme and poisoning worm, especially pine beam worm, and could be used to prepare biological nematocide. The production in said invention is characterized on high poison ability, which has better potential utility.
Owner:YUNNAN UNIV
Artificial repellent for monochamus alternatus
InactiveCN102726463ADevelop resistanceGood avoidance effectBiocidePest repellentsNematodeActive component
The invention provides an artificial repellent for monochamus alternatus, which belongs to the technical field of controlling of insects. The artificial repellent is characterized by containing 8 to 19% of active components, 5 to 12% of synergistic components, 2 to 11% of a stabilizing agent and 63 to 81% of a solvent. Through mutual synergism of a plurality of the active components which have good repellent effects, the artificial repellent for monochamus alternatus provided in the invention can substantially repel monochamus alternatus larvae boring into pine trees and monochamus alternatus adults on the surface of pine trees; therefore, carrying and propagation of pine wood nematode by the pine trees can be mitigated, the survival rate of the pine trees can be increased, overflowing of pine wood nematode can be effectively controlled, and a transmission source can be cut off. Production process for the artificial repellent is simple and convenient, and monochamus alternatus does not generate resistance even under the condition of long-term usage of the artificial repellent; and the artificial repellent has the advantages of a good repellent effect, low production cost and suitability for large-area sprinkling usage in a pine forest.
Owner:ZHEJIANG FORESTRY UNIVERSITY
Method for controlling pine wood nematode diseases by utilizing body surface bacteria of American pine wood nematodes
The invention is suitable for the field of nematode disease control and provides a method for controlling pine wood nematode diseases by utilizing body surface bacteria of American pine wood nematodes. The method comprises the steps of: spraying American bacterium suspension liquid into woodlands: spraying once in May for two years in a row, wherein the body surface pathogenic bacteria of the pine wood nematodes are replaced with American bacteria in two or three years so as to control the prevalence of woodland pine wood nematode diseases in China. According to the invention, the biological control is carried out by utilizing the replacement of ecological niche of the body surface bacteria of the pine wood nematodes and bacterial strains isolated and screened from body walls of the American pine wood nematodes. In the method, the biocontrol bacteria sprayed into the woodlands have the characteristics of low pathogenicity, strong reproduction and replacement capabilities and the like and are capable of rapidly replacing the body surface strong pathogenic bacteria of the pine wood nematodes in China to ensure that the pine wood nematodes lose the pathogenic ability and the purpose of controlling the prevalence of pine wood nematode diseases is achieved. The method has the advantages of simpleness, practicability, easiness in popularization and application, lower cost, good and thorough effect and the like.
Owner:南京绿海生物科技有限公司
Sky-ground integrated monitoring method for monitoring pine wood nematode disease
PendingCN114387528AImprove efficiencyHigh efficiency in screening epidemic areasScene recognitionData setPinewood nematode
According to the technical scheme, the sky-ground integrated monitoring method comprises the steps that S1, firstly, infected and withered trees are extracted through unmanned aerial vehicle images, plaques of the infected and withered trees are extracted through satellite images, and on-site marking, positioning and measuring are conducted on the withered trees through ground investigation; s2, respectively acquiring high-spatial-resolution satellite remote sensing data and unmanned aerial vehicle remote sensing data as data sources, and jointly forming a data set of a monitoring area in combination with ground survey data and forest resource second-class survey data; s3, establishing samples of the epidemic trees according to the data, wherein the samples comprise spectral features, texture features and geometric features of the pine wood nematode disease withered and dead trees; and S4, according to the sample and satellite remote sensing, confirming the infected, withered and dead tree plaques, comprehensively applying an image enhancement and image classification method, performing verification, and establishing spatial distribution information and position information of the pine wood nematode disease withered and dead trees. The method has the advantages of high epidemic area screening efficiency, capability of realizing large-area efficient monitoring, convenience in acquisition of remote sensing data of the unmanned aerial vehicle, high recognition precision and guarantee for precision verification.
Owner:浙江同创空间技术有限公司
Microbial insecticide for controlling wireworm and application thereof
The invention relates to a microbe nematization killing agent, which is the ferment product of photosynthetic bacterium acidophilic PSB-1 Rhodoblastus acidophilus CCTCC M 206124. Said ferment product contains the nematization killing active component as 5-Aminolevulinic acid or delta-Aminolevulinic acid whose simplified name is ALA. The inventive agent is pure biological agent, which can be used to treat rootworm, or the like, without pollution but low cost. The inventive agent will not hurt the tobacco, hot pepper, or the like, while it can accelerate and excite the growth of crops.
Owner:HUNAN PLANT PROTECTION INST
Method for controlling pipe wilt disease by forecasting and mass-trapping of monochamus alternatus and monochamus saltuarius
ActiveCN105530811ASustainable lureEffective lureBiocidePest attractantsNematodeMonochamus saltuarius
The present invention relates to a method for controlling pine wood nematode by installing, at regular intervals, net type traps including an attractant composition for attracting Monochamus alternatus and Monochamus saltuarius, which are pollinating insects of pine wood nematode, and capturing, within the traps, the attracted Monochamus alternatus and Monochamus saltuarius. The present invention can constantly and effectively capture Monochamus alternatus and Monochamus saltuarius over a whole generation by forming a net-type trap system at a control area on the basis of the physiological, ecological, and behavioral characteristics of Monochamus alternatus and Monochamus saltuarius which do not show active scattering mobility, and thus can control the damage caused by pine wood nematode through the medium of Monochamus alternatus and Monochamus saltuarius and can forecast whether or not damage will occur.
Owner:AD公司
Method for comprehensively preventing and treating longhorn beetles
InactiveCN107667739AHigh kill rateFacilitated DiffusionPlant protectionInsect catchers and killersNematodeLonghorn beetle
The invention relates to the technical field of insect pest prevention and treatment in forestry, in particular to a method for comprehensively preventing and treating longhorn beetles. In the method,first, healthy masson pine wood is selected, pine wood nematodes are introduced and utilized for infecting the masson pine, then the infected masson pine and beauveria bassiana are blended and subjected to anaerobic digestion, yeast is obtained, monoterpene pheromones of the pine wood nematodes are carried in the masson pine yeast after infection of the pine wood nematodes, the pine wood nematodes are hosts of the longhorn beetles, the pheromones of the hosts will lure and gather the longhorn beetles, the lured and gathered longhorn beetles will make contact with beauveria bassiana, in this way, the longhorn beetles and larvae of the longhorn beetles can be effectively killed, the longhorn beetles will also carry the deadly beauveria bassiana to other places to promote diffusion of the longhorn beetle deadly bacteria, and then the killing rate is further increased for the longhorn beetles. Sclerodermus harmandi as parasitical natural enemies of the longhorn beetles is released aroundluring wood filled with self-made luring and killing agents and is parasitic on the larvae of the longhorn beetles to further increase the killing rate for the longhorn beetles, finally, the luring wood is burned down in a concentrated mode, the longhorn beetles are thoroughly killed, and broad application prospects are achieved.
Owner:陈合华
Pesticide for pine material nematode
InactiveCN1554238AImprove permeabilityHigh insecticidal activityBiocideAnimal repellantsNematodeAbamectin
The pesticide for killing pine material nematode consists of abamectin 0.001-0.1 wt%, copper sulfate 30-35 wt%, Shenzhanling 1-3 wt% and water the rest. Of the components, copper sulfate can kill separated nematode directly in high effect, wide killing spectrum and lasting effect, Shenzhanling makes copper sulfate permeate into pine material to kill nematode, and abamectin can kill nematode and black pine longicorn. The present invention has the advantages of high permeation, high efficiency, last effect, safety and convenience.
Owner:陈纪文
Attractant for entrapping imagoes of arhopalus rusticus
InactiveCN101933511AGood lure effectSimple preparation processBiocidePest attractantsDiseasePinewood nematode
The invention provides an attractant for entrapping imagoes of arhopalus rusticus, the attractant comprises the components including 3-carene and absolute ethyl alcohol in a volume ratio of 0.8-1.2:1. The attractant has advantages of simple preparation technique, obvious entrapping effect on arhopalus rusticus, no pollution, environmental friendliness, good entrapping persistence and the like; the attractant is used together with polyethylene thin-walled slowly-releasing plastic bottles and black plastic cross traps, and has an excellent entrapping effect on imagoes of arhopalus rusticus; theattractant is capable of predicting, forecasting, preventing and treating arhopalus rusticus, as well as effectively controlling the density of arhopalus rusticus in forest and preventing the spread and expansion of nematode disease for pine; therefore, the attractant provides an important technical and product support for the comprehensive treatment of pine wood ematode disease, pine wood mucronatus and arhopalus rusticus.
Owner:YUNNAN ACAD OF FORESTRY
Kit for qualitative detection of pinewood nematode and detection method thereof
ActiveCN102827933AIncrease concentrationOptimizing the Reaction TemperatureMicrobiological testing/measurementPositive controlA-DNA
The invention discloses a kit for qualitative detection of pinewood nematode and a detection method thereof. Reagents of the kit comprise a DNA extract, a reaction liquid of constant-temperature amplification of pinewood nematode nucleic acid, a positive control and a negative control, wherein the positive control is a plasmid containing a DNA fragment of a pinewood nematode BxS1 gene, and the negative control is sterile double distilled water. The kit detects the pinewood nematode through constant-temperature amplification of nucleic acids, and sensitivity thereof is that 100 copies can be detected in each reaction system. Repetitive experiments show that detection results have no significant differences and are good in repeatability. Only 2 hours are required to finish the sample detection, thereby greatly reducing detection time. Meanwhile, only one person is needed to finish all operation process of the kit, and one to hundreds of samples can be detected for one time, thereby reducing manpower waste, meeting requirements of rapid detection of the pinewood nematode and thus being quite practical.
Owner:NANJING FORESTRY UNIV +1
Satellite monitoring spatial database of dead pinus massoniana infected with pine wilt disease
InactiveCN109360376AReal-time monitoring of air temperatureMonitor humidity in real timeColor/spectral properties measurementsAlarmsPlant diseaseOrganism
The invention discloses a satellite monitoring spatial database of dead pinus massoniana infected with a pine wilt disease. The satellite monitoring spatial database of the dead pinus massoniana infected with the pine wilt disease comprises (1) spectral information and environmental information of the pinus massoniana are collected through a satellite remote sensing image; (2) the collected spectral information is transmitted to a data receiving unit; (3) spectral data of the data receiving unit is extracted to obtain a characteristic parameter, and the characteristic parameter is processed toobtain a spectral index; (4) characteristic matching and recognizing processing is carried out on the spectral index and pine wilt disease characteristic data through a pine wilt disease forecastingunit to obtain pine wilt disease degree information of the pinus massoniana; and (5) the pine wilt disease degree information of the pinus massoniana is transmitted to an early warning release unit. An early warning method of the pine wilt disease of the pinus massoniana is simple in processes, the pine wilt disease of the pinus massoniana monitoring and early warning can be accurately carried outin a target area, the air temperature, humidity, pinus massoniana images and other parameters of the pinus massoniana can be monitored in real time, and the growth situation of the pinus massoniana can be automatically and rapidly monitored.
Owner:航天信德智图(北京)科技有限公司
Application of alpha-trithiophene in control of pine wood nematode disease
InactiveCN1736194AHighly toxicExhibits photoactivatable propertiesBiocideAnimal repellantsNematodePinewood nematode
The invention relates to the use of alpha-terthiophene, in particular the use for the control of pine wilt disease, experimental investigation has shown that, when the when the concentration of the alpha-terthiophene is higher than 10-4g / L, the pine wilts can be poisoned within short period of time. The alpha-terthiophene can be applied to pine trees directly or with the compositions containing the alpha-terthiophene.
Owner:赵昕
Method for plant regeneration and somatic embryogenesis of masson pine capable of resisting against pine wilt diseases
PendingCN107593454AConvenient inductionPromote rootingPlant tissue cultureHorticulture methodsSomatic embryogenesisMasson pine
The invention discloses a method for plant regeneration and somatic embryogenesis of masson pine capable of resisting against pine wilt diseases. The method comprises the following steps: by taking immature zygotic embryo of the masson pine capable of resisting against pine wilt diseases as a material, performing embryonic callus induction, embryonic callus multiplication, maintenance, differentiation, maturation and germination; performing rooting induction on the somatic embryo; performing strong seedling culture and domestication transplanting on the regenerated plant; and lastly, acquiringthe masson pine plant capable of resisting against pine wilt diseases. According to the method for plant regeneration and somatic embryogenesis of masson pine capable of resisting against pine wilt diseases disclosed by the invention, the immature zygotic embryo of the resistant masson pine is taken as the material, a resistant masson pine somatic embryogenesis regeneration system is preliminarily established, the embryonic callus induction for the resistant masson pine is optimized, the important links, such as, somatic embryo multiplication, differentiation, germination and regeneration plant transplanting domestication, are further researched, a small quantity of resistant masson pine suspension culture and somatic embryogenesis regenerating plants are cultivated and an excellent technical basis is established for the industrialized production of the resistant masson pine.
Owner:NANJING FORESTRY UNIV
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