40results about How to "Control epidemic" patented technology

The invention relates to a bacillus amyloliquefaciens strain, which is characterized in that the collection No. of the strain is CGMCC No.3789; and the 16S rRNA of the strain accesses to GenBank, with accession No. of HQ179100. The strain is used for controlling rice bacterial leaf streak. The strain has the following advantages: the strain has good control effect on the rice bacterial leaf streak, is free of toxin and pathogenicity, is harmless to people and livestock and is environment-friendly; the prepared biocontrol bacteria or the compound biocontrol bacteria formed by compounding the prepared biocontrol bacteria and bismerthiazol are sprayed after being diluted, so that reasonable distribution of the microfloras in the ecological environment around the root leaves of the rice can be controlled and managed and the living environment of the pathogenic bacteria is worsened to form a rice ecosystem with biodiversity, thus effectively and enduringly controlling prevalence of the rice bacterial leaf streak.

The invention provides bacillus amyloliquefaciens SZ-60 which is characterized in that the collection number is CGMCC No.8277. The bacterial colony formed by the strain on a beef-extract peptone (NB) medium by virtue of single-cell reproduction is irregular with oyster white color, slight upheaval at the center and wet and semitransparent surface; the microscopy is of a rod shape, and the size is (0.3-0.4)*(3.2-3.3)microns; with flagella, G- and spores, the strain can grow on the beef-extract peptone (NB) medium containing 2-5% of NaCl; the formula of the beef-extract peptone (NB) medium contains 3.0g of beef extract, 10.0g of peptone, 5.0g of NaCl, 17g of agar and 1,000ml of water, and the pH is 6.8-7.2. The bacillus amyloliquefaciens realizes a remarkable antagonistic action on the main pathogenic bacteria causing ginseng root rot, epidemic diseases, sclerotinia sclerotiorum, cylindrocarpon destructans, black spot and damping off. The invention also provides an application of the bacillus amyloliquefaciens SZ-60 in preventing plant fungal diseases, or application in preparing a microbial preparation for preventing plant fungal diseases.

The present invention is recompounded microbe germicide for preventing and controlling banded sclerotial blight and green smut of rice. The recompounded microbe germicide is recompounded through thefermentation culture of Bacillus subtilis and recompounding the fermented liquid with certain content of Bacillus subtilis and Jinggangmeisu. It is used in preventing and controlling banded sclerotial blight and green smut of rice in field via utilizing the complementary superiority of Bacillus subtilis and Jinggangmeisu. It has high functions of preventing and controlling banded sclerotial blight and green smut of rice, less influence on rice quality, stable germicidal effect and long preservation period.

The invention provides bacillus methylotrophicus SZ-3 and SZ-81, with collection numbers of CGMCC No.8275 and CGMCC No.8276 respectively. The bacillus methylotrophicus SZ-3 has obvious antagonistic effects on pathogenic bacteria causing root rot, black spot and sclerotinia sclerotiorum of ginseng. The bacillus methylotrophicus SZ-81 has obvious antagonistic effects on pathogenic bacteria causing epidemic diseases, root rust rot and black spot of panax ginseng. The invention also provides an application of the bacillus methylotrophicus SZ-3 and SZ-81 to control of fungal diseases of plants or an application to preparation of microbial preparations for controlling fungal diseases of plants.

The invention relates to a bacillus amyloliquefaciens B-1619 strain and application in preventing and controlling soil-borne disease of nightshade vegetables thereof. The preservation number of the strain is CGMCC No. 4671. When the strain is applied, biocontrol strain powder is mixed with soil before seeding, and soil with biocontrol strain is covered on seeds after seeding; the biocontrol strain powder is firstly planted on the surface of the soil before seed separation; diluted biocontrol strain liquid is aligned to seeds of nightshade vegetables to be planted before planting, and the diluted biocontrol strain liquid is irrigated to the roots of the plants of nightshade vegetables during the planting; the diluted biocontrol strain liquid is irrigated to the roots of the plants of nightshade vegetables periodically after the planting; or, the application ways in each period from the seeding to the harvesting period of the nightshade vegetables are combined.

The invention provides a smoked plum extractive and wild jujube seed extractive compound preparation, comprising the following components according to parts by weight: smoked plum alcohol 49-90, wild jujube seed alcohol 9-50, and menthol crystal 0.2-4.5. The smoked plum extractive and wild jujube seed extractive compound preparation is prepared into soluble powder, granule, oral liquid, injection or lyophilized powder for injection by extracting smoked plum and wild jujube seed with 70-75% alcohol in a proper way to obtain crude extract and regular auxiliary material or carrier according to regular preparation method. The compound preparation can be applied in preparing medicament for preventing and curing poultry and livestock virus diseases or bacteria diseases, having the characteristics of good curing effect, quick effect, high security, no residue, environment protection and the like.

The invention is suitable for the field of nematode disease control and provides a method for controlling pine wood nematode diseases by utilizing body surface bacteria of American pine wood nematodes. The method comprises the steps of: spraying American bacterium suspension liquid into woodlands: spraying once in May for two years in a row, wherein the body surface pathogenic bacteria of the pine wood nematodes are replaced with American bacteria in two or three years so as to control the prevalence of woodland pine wood nematode diseases in China. According to the invention, the biological control is carried out by utilizing the replacement of ecological niche of the body surface bacteria of the pine wood nematodes and bacterial strains isolated and screened from body walls of the American pine wood nematodes. In the method, the biocontrol bacteria sprayed into the woodlands have the characteristics of low pathogenicity, strong reproduction and replacement capabilities and the like and are capable of rapidly replacing the body surface strong pathogenic bacteria of the pine wood nematodes in China to ensure that the pine wood nematodes lose the pathogenic ability and the purpose of controlling the prevalence of pine wood nematode diseases is achieved. The method has the advantages of simpleness, practicability, easiness in popularization and application, lower cost, good and thorough effect and the like.

The invention discloses a trichoderma preparation for controlling pepper phytophthora blight and a field tank mix pesticide containing the same. The trichoderma preparation comprises, by weight: 28-32% of Trichoderma conidia, 10-15% of a dispersant, 4-6% of a wetting agent, 1-2% of a protective agent, and 50-55% of a carrier, wherein the trichoderma is Trichoderma. asperellum CGMCC No.6422. The field tank mix pesticide is prepared by mixing the trichoderma preparation and a bactericide and dissolving the mixture in water during application. The bactericide is eugenol or mandipropamid. The trichoderma preparation provided in the invention has the advantages of good dispersing effect, long storage period, and good pepper phytophthora blight control effect. In addition, the field tank mix pesticide developed in the invention can achieve a good control effect with a reduced number of times of pesticide delivery to phytophthora capsici, the control cost can be lowered, and pollution of reagents to the environment is reduced.

The invention discloses a train compartment air purification system, and relates to air purification equipment. According to the technical scheme, the system consists of an air purification unit and an air quality detection display unit; an air inlet of a central air conditioner in the train compartment is provided with a purification device, the air in the compartment and the fresh air in the train are mixed in an air mixing box and then continuously circulate to enter the purification device; after being purified, the air is sent to each air outlet through a pipeline of the central air conditioner, so that the purpose of purification is achieved; the purification device is provided with a purification material containing polydopamine and silver ions, and the polydopamine is used for removing TVOC, the silver ions are used for killing and inhibiting germs; the air quality detection module is used for detecting the air quality in the compartment in real time, an air quality display screen displays main indexes of the air pollutants in the compartment in real time and transmits the main indexes to a main train department in real time. The system has the beneficial effects that the air quality of train compartments such as high-speed railway, subways, urban rails and the like can be effectively improved.

The invention discloses a fast detection method for high-resistance botrytis cinerea bacterial strains of carbendazim. The method can be used for performing dynamic monitoring and early warning on botrytis cinerea bacteria causing gray mold of crops for the resistance groups of the carbendazol. In the method disclosed by the invention, a molecular detection technology having the advantages of fastness, convenience, strong specificity, high sensitivity and low cost is established on the basis of a loop-mediated isothermal amplification technology (LAMP). The detection method comprises the following steps: designing 2 pairs of specific primers on beta tubulin of high-resistance bacterial strains of the botrytis cinerea bacteria for the carbendazol, performing LAMP amplification, and judging whether products are the high-resistance bacterial strains of the botrytis cinerea bacteria for the carbendazol or not according to the colors of reaction products. If the LAMP amplification products appear to be azure, electrophoresis patterns present ladder-shaped strips, and the product amplification is generated, the products are identified as the high-resistance bacterial strains of the botrytis cinerea bacteria for the carbendazol; if the LAMP amplification products present violet, the electrophoresis patterns do not have strips, and the product amplification is not generated, then the products are identified as non high-resistance bacterial strains of the botrytis cinerea bacteria for the carbendazol. The method is simple, convenient, fast, and low in cost and has important actual significance in the resistance risk assessment of the gray mold of the crops and reasonable medication.

The invention discloses a gridding rice sheath blight habitat evaluation method integrating multi-source remote sensing information. Prediction only depends on punctiform meteorological data and is relatively extensive, and a continuous planar prediction result cannot be provided. The method comprises the following steps: 1, selecting an investigation area, acquiring multi-source remote sensing data of the investigation area and investigating disease severity of the investigation area; 2, determining the position and area of late rice; and 3, extracting rice sheath blight habitat characteristics based on the multi-source remote sensing image. And 4, establishing a disease habitat suitability model. And 5, gridding the measured field, and extracting the remote sensing habitat characteristics of the rice sheath blight disease. And 6, masking each remote sensing habitat feature by using the late rice classification map to obtain each remote sensing habitat factor feature of the late rice.And 7, acquiring the disease habitat suitability degree corresponding to each grid according to the model. And 8, obtaining a disease habitat suitability spatial distribution map. Through investigation and research analysis, most of soil-borne diseases are found to be related to habitat conditions such as host plant growth state and farmland environment.

The invention discloses fermentation liquor for producing extracellular antifungal protein produced by using Bacillus amyloliquefaciens SZ-60 and a fermentation method of the fermentation liquor. A fermentation culture solution which contains 1000 mL of deionized water contains 10-20 g of soluble starch, 10-20 g of soybean meal, 2-7 g of NaCl, 2-7 mg of MnSO4, 5-15 mg of Ca(HCO3)2 and 1-4 mg of FeSO4, wherein the pH value of the fermentation culture solution is 6.5-7.5. The fermentation culture condition combination of the Bacillus amyloliquefaciens SZ-60 for producing antibacterial protein substances is optimized, and technical support and theoretical guidance are provided for industrial production of the Bacillus amyloliquefaciens SZ-60 strain and acquirement of high yield of the antifungal protein substances. An NB60YF microbial preparation prepared by the fermentation of the Bacillus amyloliquefaciens SZ-60 has a good prevention and treatment effect on gray mold caused by Botrytiscinerea.

The present invention provides Bacillus vallismortis SZ-4, which has the preservation number of CGMCC No.8273. According to the present invention, the strain grows on a beef extract-peptone (NB) culture medium in a single cell reproduction growth manner to form bacterial colonies, and the bacterial colonies have characteristics of round shape, milk white color, slightly elevated center, and moist and transparent surface; microscopic examination results show that the Bacillus vallismortis SZ-4 has a rod shape, has flagella, has a size of 0.7-0.82*1.6-2.2 mum, presents the positive Gram staining effect, has spores, and can grow on a beef extract-peptone (NB) culture medium containing 2-5% of NaCl, wherein the beef extract-peptone (NB) culture medium comprises: 3.0 g of a beef extract, 10.0 g of peptone, 5.0 g of NaCl, 17 g of agar and 1000 ml of water, and the pH value is 6.8-7.2; and the Bacillus vallismortis SZ-4 provides significant antagonism effects for pathogenic bacteria causing ginseng root rot, blight, sclerotinia sclerotiorum and botrytis cinerea. The present invention further provides an application of the Bacillus vallismortis SZ-4 in prevention and control of plant fungal diseases or preparation of microorganism preparations for prevention and control of plant fungal diseases.

The invention involves nephrotic syndrome hemorrhagic fever Vero cells purified bivalent vaccine and its industrialized preparation methods. The nephrotic syndrome hemorrhagic fever Vero cells bivalent purified vaccine in the inventioncontains effective doses of nephrotic syndrome hemorrhagic fever deactive virus type I, type II and vaccine adjuvants, the type I and type II virus are from PS-6 (C-3) strains, CCTCC-V200503 and L99(C-2) strains, CCTCC-V200504 respectively. The preparation method of the invention includes: 1. Preparating the vaccine stock solution nephrotic syndrome hemorrhagic fever virus PS-6(C-3), CCTCC-V200503 and L99(C-2),CCTCC-V200504, inactivating virus with formaldehyde; 2. combining the deactive vaccine stock solution of PS-6(C-3), CCTCC-V200503 and L99(C-2),CCTCC-V200504 and purifying, the process contains centrifugal separation, hyperfiltration, concentration, zinc acetate deposition and column chromatography; 3. adding adjuvant into the purified vaccine stock solution and getting the purified bivalent vaccine. The nephrotic syndrome hemorrhagic fever Vero cells purified bivalent vaccine in the invention is suitable for the prevention of nephrotic syndrome hemorrhagic fever mainly based on all type I and type II.

The present invention discloses a method for preventing and controlling porcine epidemic diarrhea. A return feeding method is adopted, a natural infection method is simulated, feces or intestinal tract and intestinal contents of porcine epidemic diarrhea virus (PEDV) infected animals are crushed, ground and diluted, then antibiotics are added, non-infected animals are infected through mouths, the total group can be infected in the same period, mucosal immunoreaction is stimulated to be generated, IgA antibodies are generated, so that the anti-PEDV effect is achieved, and finally the pestilence can be controlled. The return feeding method is tried out through a small group, the specificity sIgA antibody level is detected by using an indirect ELASA method, the return feeding dosage is determined according to antibody level change conditions, then large group return feeding is carried out, and return feeding failure caused by the fact that the return feeding dosage is not exact in the past is largely reduced. According to the method, the porcine epidemic diarrhea can be effectively prevented and controlled, the spread of diseases can be rapidly and effectively controlled especially when the epidemic diarrhea diseases outbreak, and greater economic losses and disease outbreak again can be prevented; and the return feeding process is simple and easy to operate, and cost is low.

The invention relates to an all-in-one machine for dovecote electric feeding, disinfection and immunity. The all-in-one machine includes an electric traveling mechanism, a feeding mechanism, a disinfection mechanism and an immune mechanism. The immune mechanism includes a control switch three, a connecting pipe, a vaccine dilution bottle, a spray head and a vaccine spray immunity box. The vaccine spray immunity The box is in the middle and upper part of the frame and is located above the water tank. The connecting pipe is connected to the vaccine dilution bottle, and a control switch 3 is set on the connecting pipe. The vaccine dilution bottle is connected to a water pipe, and a nozzle is arranged on the water pipe. The vaccine Both the dilution bottle and the spray head are arranged in the vaccine spray immunization box. The invention has the advantages of simple structure, convenient cleaning, labor saving and material saving, and various uses.

The present invention relates to a construction and an application of a A19 Brucella VjbR deletion mutant strain and belongs to the field of Brucella vaccine research. By designing PCR primers for upstream and downstream regions of VjbR and kana resistance genes, the upstream and downstream two length homologous nucleotide fragments and kana resistance genes of a target gene are amplified and directionally connected together by a PCR amplification method to obtain target gene-deficient type homologous nucleotide fragments, the obtained target gene-deficient type homologous nucleotide fragmentsare linked to cloning vectors to obtain the target gene-deficient type homologous nucleotide fragment-containing recombinant vectors, the constructed target gene-deficient type homologous nucleotidefragment-containing recombinant vectors are transformed into Brucella A19, and positive clones are screened to obtain a target gene-deficient Brucella A19 attenuated vaccine mutant strain. The vaccinemutant strain is expected to develop into a labeled attenuated vaccine and play positive promotion roles in controlling spreading of brucellosis and healthy development of national economy.

The invention relates to construction and application of an A19 Brucella GroES deleted mutant strain and belongs to the field of Brucella vaccine research. By designing PCR primers of an upstream region, a downstream region and a kana resistance gene of GroES, amplifying homologous nucleotide fragments of two lengths of the upstream and the downstream of a target gene and the kana resistance gene,directionally connecting the homologous nucleotide fragments with the kana resistance gene by adopting a PCR amplification method, defective homologous nucleotide fragments of the target gene are obtained, and the obtained defective homologous nucleotide fragments of the target gene are connected into a cloning vector to obtain a recombinant vector containing the defective homologous nucleotide fragments of the target gene, the constructed recombinant vector containing the defective homologous nucleotide fragments of the target gene is transformed into Brucella A19, positive clones are screened to obtain the Brucella A19 attenuated vaccine mutant strain of the deleted target gene. The vaccine mutant strain is expected to be developed into a marked attenuated vaccine, and plays a positivepromoting role in controlling the epidemic and transmission of brucellosis and the healthy development of national economy.

The invention is a technical route that a full physical method is adopted for vegetable pest control, which is characterized in that: firstly, soil 1 being subjected to water washing, detoxication, and disinfection of ozone is subjected to heating deinsectization process after soil testing and formulated fertilization, so as to obtain full nutrient clean soil 2; the soil 2 is put in a nontoxic, moisture holding, and air permeable container 3; a healthy seed or seedling is planted in the soil 2 in the container 3 to obtain healthy vegetable 4; cultivation environment of temperature, moisture, illumination and the like are adjusted and controlled to satisfy physiologic requirements of the vegetable 4, and to reduce physiologic diseases 5 and infective diseases 6 caused by the physiologic diseases 5; secondly, the physical methods of fluorescent trapping, colour plate trapping, and the like are utilized to reduce pest damages 7 and the infective diseases propagated by pests; thirdly, the growth situation of the vegetable 4 is monitored, if a diseased plant 8 suffered from the infective diseases 6 is found, the diseased plant 8 along with the cultivation container 3 and the soil 2 are moved to be far away from healthy groups 9 of the vegetable 4, so that the epidemic of the infective diseases 6 among the healthy groups 9 is controlled.

The invention relates to a preparation method of a C-type clostridium perfringens toxin vaccine of piglet red dysentery. The preparation method comprises the following steps of inoculating strains NCTC (National Collection Of Type Culture) 3180 of the C-type clostridium perfringens into a blood plate culture medium for carrying out anaerobic cultivation for 36 hours at 37 DEG C; carrying out shake cultivation on the culture by adopting a homemade toxin-producing medium (100 ml of PBS (Phosphate Buffer Solution), 2g of peptones, 1g of dextrin, 2g of yeast extracts, and 1.2g of L- arginine) under an anaerobic environment; inactivating the culture, filtering and adding an adjuvant to prepare the C-type clostridium perfringens toxin vaccine of the piglet red dysentery. During a using process, intramuscular injection is respectively adopted once a month and half a month before the delivery of a sow, wherein the dosage is 5-10 ml at a time. The C-type clostridium perfringens toxin vaccine of the piglet red dysentery is used for the sow at the later immunological gestation period, so that piglets obtain passive immunity through colostrums, and therefore, piglet red dysentery can be prevented. Moreover, the C-type clostridium perfringens toxin vaccine of the piglet red dysentery can be used for preventing sheep struck and calf enterotoksemia, so that the popularization of the C-type clostridium perfringens disease can be effectively controlled.

The invention discloses a viral acute infectious disease prevention and control method based on the disease control theory. The method accords with the objective law of occurrence and development of diseases. The highest efficiency, the optimal effect and the purposes of controlling prevalence of the diseases, decreasing the morbidity, decreasing the death rate and the like can be achieved through cooperation, mechanism innovation, management control and other means according to requirements of the disease control theory.

The invention relates to a new usage of citric acid and compound citric acid in the field of pharmacy. The product can prevent and control viral diseases and bacterial diseases of livestock and poultry; wherein, the compound citric acid comprises the following components according to weight portion: 93-98 portions of citric acid, 0.5-4 portions of gualfenesin and 1.5-3 portions of etamsylate. Proved by in vivo and vitro experiments, the compound citric acid can play a role in preventing and controlling fowl mycoplasma gallisepticum, avian influenza (H9 subtype), bursal virus disease and otherdiseases, and can effectively kill animal pathogenic bacterias such as cattle colon bacillus, mycoplasma gallisepticum, chicken colibacillosis, etc.

The invention discloses a method for preventing and treating the pine wilt disease by using American pine wood nematode body surface bacteria. The method comprises the following steps: separation of nematodes and bacteria: separating nematodes in a wood block to be tested by adopting a Belman funnel method, and separating bacteria on body surface of the nematodes by adopting a dilution separation method; preparation of a bacterial suspension; preparation of a bacterial cell-free culture solution; and application of a bacterial cell-free filtrate: applying the bacterial cell-free filtrate according to the size of the diameter at breast height of a pine tree, wherein the use frequency of the bacterial cell-free filtrate is 2 years per time. According to the method for preventing and treating the pine wilt disease by using the American pine wood nematode body surface bacteria, a pesticide application amount is determined according to the size of the diameter at breast height of the pine tree, and the ecological niche of the pine wood nematode body surface bacteria is replaced, so the prevalence rate of the pine tree is effectively reduced, and the survival rate of the pine tree is improved; a prevention and treatment period is prolonged, and the disease can be prevented for 2 to 3 years as the bacterial cell-free filtrate is applied once; and the method is easy to operate and low in cost, and the survival rate of the pine tree reaches 99% when the pine tree is treated with the method.

The invention discloses a fermentation culture solution for producing extracellular antifungal protein by using Bacillus vallismortis SZ-4 and a fermentation method of the fermentation culture solution. The fermentation culture solution which contains 1000mL of deionized water contains 7-15 g of glucose, 15-25 g of rapeseed powder, 1.5-4.5 g of NaCl, 1.0-3.0 mg of MnSO4.7H2O, 2.5-7.5 mg of MgSO4.H2O, 1.0-3.0 mg of Ca(HCO3)2 and 2.5-7.5 mg of KH2PO4, wherein the pH value of the fermentation culture solution is 5.5-7. The fermentation culture condition combination of the Bacillus vallismortis SZ-4 for producing antifungal protein substances is optimized, and technical support and theoretical guidance are provided for industrial production of the Bacillus vallismortis SZ-4 and acquirement ofhigh yield of the antifungal protein substances. A GR4YF microbial preparation prepared by the fermentation of the Bacillus vallismortis SZ-4 has a good prevention and treatment effect on ginseng rootrot.

The invention relates to a schistosomiasis protective agent used for cattle, namely niclosamide spraying agent and a preparation method thereof, belonging to the technical field of protective agent medicines used for preventing cattle from being infected by schistosomiasis. In parts by mass, 0.5-5 parts of niclosamide is dissolved into 5-20 parts of cosolvent, 0.5-2 parts of penetrant and 1-5 parts of emulsifier are added, then absolute ethyl alcohol is added under the stirring condition to prepare solution, and the total mass of the solution is 100 part, thus the niclosamide spraying agent is obtained. The spraying agent is sprayed onto the skin of cattle to form a medicine protective layer, the cattle can be effectively prevented from being infected by schistosome in 15-30 days, spread of schistosomiasis is controlled, and human health is protected while sustainable development of animal husbandry is promoted. The niclosamide spraying agent provided by the invention is simple in preparation method, is convenient to use and can prevent and control prevalence of schistosomiasis among people while the cattle is protected from being infected by schistosome.

The invention relates to a bacillus amyloliquefaciens strain, which is characterized in that the collection No. of the strain is CGMCC No.3789; and the 16S rRNA of the strain accesses to GenBank, with accession No. of HQ179100. The strain is used for controlling rice bacterial leaf streak. The strain has the following advantages: the strain has good control effect on the rice bacterial leaf streak, is free of toxin and pathogenicity, is harmless to people and livestock and is environment-friendly; the prepared biocontrol bacteria or the compound biocontrol bacteria formed by compounding the prepared biocontrol bacteria and bismerthiazol are sprayed after being diluted, so that reasonable distribution of the microfloras in the ecological environment around the root leaves of the rice can be controlled and managed and the living environment of the pathogenic bacteria is worsened to form a rice ecosystem with biodiversity, thus effectively and enduringly controlling prevalence of the rice bacterial leaf streak.