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Bacterial strain Smal-007 for controlling pine wilt disease and application of bacterial strain

A technology for pine wood nematode disease and pine wood nematode, applied in the field of bacterial strains, can solve the problems of incomplete felling, large tree damage, and inability to completely solve pine wood nematode disease

Active Publication Date: 2013-07-17
南京绿海生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this method is that the felling is not complete, and it happens every year. ②The trunk is injected with nematicides to protect the precious pine trees
The disadvantage of this method is that it is cumbersome to use and causes great damage to trees
③Using paras

Method used

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  • Bacterial strain Smal-007 for controlling pine wilt disease and application of bacterial strain
  • Bacterial strain Smal-007 for controlling pine wilt disease and application of bacterial strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] 1. Preparation medium: glucose 2%, sucrose 2%, peptone yeast extract complex 3% (g / ml).

[0013] 2. Fermentation culture: Add the strain Smal-007 into the culture medium for submerged fermentation culture, the fermentation ventilation rate is 15L / m, the stirring speed is 200r / min, the initial pH value is 7.0, the fermentation temperature is 30°C, and the fermentation time is 36h. OD value 14.

[0014] 3. Separate packaging and transportation of the bacterial agent: the bacterial agent is packaged in plastic barrels or bottles, and the temperature of the bacterial agent during transportation should not exceed 35°C.

[0015] 4. Spraying fungicides in the forest:

[0016] (1) Aircraft spraying: Spray the original bacteria solution, 400 grams per mu. Each sortie has a drug load of 200 kg and sprays 500 mu.

[0017] (2) Spraying with a motorized sprayer: Spraying the original bacteria solution, spraying 500-1000 grams per mu. Focus on spraying the trunks of dead wood pin...

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PUM

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Abstract

The invention relates to a bacterial strain Smal-007 for controlling a pine wilt disease and an application of the bacterial strain. The bacterial strain is Stenotrophomonas maltophilia and has a preservation number of CGMCC (China General Microbiological Culture Collection Center) NO.6990. A 16s rDNA (ribosome deoxyribonucleic acid) base sequence of the strain is shown as SEQ ID No: 1. The invention further relates to fermentable culture and woodland application of the strain. The application comprises the specific steps of: (1) a fermentation medium formula; (2) a fermentation method (comprising fermentation parameters and technology); (3) a bactericide subpackaging and transporting technology; and (4) a bactericide woodland spraying technology (a woodland control technology).

Description

technical field [0001] The invention relates to a bacterial strain, in particular to a bacterial strain for preventing and treating pine wood nematode disease. Background technique [0002] Pine wood nematode is the most serious tree disease in the world. The disease has occurred in Japan, South Korea, the United States, Canada, Mexico and other countries. Japan is the most serious country for pine wood nematodes, almost causing the destruction of red pine forests and black pine forests. Since the first discovery of pine wood nematode disease in Jiangsu Province in 1982 in my country, the epidemic area has expanded to 192 county-level administrative regions in 14 provinces (autonomous regions and municipalities), killing more than 500 million pine trees and destroying more than 5 million mu of pine forests. Caused economic losses of hundreds of billions of dollars. Although the research on pine wood nematode disease in our country has been going on for decades, so far, we s...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P5/00C12R1/01
Inventor 韩正敏曾腓力贲爱玲刘侃诚孙京天糜文琪
Owner 南京绿海生物科技有限公司
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