Recompounded microbe germicide for preventing and controlling banded sclerotial blight and green smut of rice
A rice sheath blight and microbial technology, applied in the fields of application, biocide, animal repellent, etc., can solve the problems of no microbial preparations, microbial preparations and chemical pesticides, no high-efficiency Bacillus subtilis, etc. Achieve sustainable disease control, rapid antibacterial effect, and reduce the incidence of disease
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Embodiment 1
[0019] Implementation example 1, Bacillus subtilis B-916 fermented liquid and Jinggangmycin are compounded into the product of the present invention: the spore content is greater than or equal to 90% of the thalline and the bacterium content is greater than or equal to 2 × 10 10 cfu / ml Bacillus subtilis B-916 fermentation broth and 5% Jinggangmycin are compounded according to the volume ratio of 1:1, and stirred evenly to obtain the microbial compounded pesticide product (wenquning) of the present invention. A benzoic acid preservative accounting for 2‰ of the total volume can be added to the above-mentioned microbial compound pesticide to prevent the agent from spoiling. After adding the benzoic acid preservative, stir evenly, and then pack.
Embodiment 2
[0020] Implementation example 2, the industrial production method of microbial composite pesticide product (ventroning) of the present invention: first according to routine method, in laboratory, bacillus subtilis bacterial strain B-916 is activated, then carries out bacterial strain cultivation according to conventional method in laboratory, Transfer the activated strain into 500ml of YPG (tryptone 5g, yeast extract 5g, glucose 5g, water 1000ml) culture solution, culture at 120 rpm, 28°C for 48 hours, according to the inoculum size 1:150, pH value 7.0-7.4 ,) for fermentation culture, the fermentation time is 18-48 hours, during which the seed tank is tested once every 2 hours, when the bacteria content is greater than or equal to 10 10 For cfu / ml, according to the inoculum size 1:150-1:300, transfer the fermented liquid in the seed tank to a large fermenter for fermentation (temperature 28-32°C, pH value 7.0-7.4, and appropriate amount of disinfectant can be added when there a...
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