Patents
Literature
Hiro is an intelligent assistant for R&D personnel, combined with Patent DNA, to facilitate innovative research.
Hiro

38results about How to "Control happens" patented technology

Bacillus amyloliquefaciens B-1619 strain and application in preventing and controlling soil-borne disease of nightshade vegetables thereof

The invention relates to a bacillus amyloliquefaciens B-1619 strain and application in preventing and controlling soil-borne disease of nightshade vegetables thereof. The preservation number of the strain is CGMCC No. 4671. When the strain is applied, biocontrol strain powder is mixed with soil before seeding, and soil with biocontrol strain is covered on seeds after seeding; the biocontrol strain powder is firstly planted on the surface of the soil before seed separation; diluted biocontrol strain liquid is aligned to seeds of nightshade vegetables to be planted before planting, and the diluted biocontrol strain liquid is irrigated to the roots of the plants of nightshade vegetables during the planting; the diluted biocontrol strain liquid is irrigated to the roots of the plants of nightshade vegetables periodically after the planting; or, the application ways in each period from the seeding to the harvesting period of the nightshade vegetables are combined.
Owner:JIANGSU ACADEMY OF AGRICULTURAL SCIENCES

Reactive moisture-curable polyurethane hot melt adhesive and preparation method thereof

The invention belongs to the field of polymer chemistry, and particularly relates to a reactive moisture-curable polyurethane hot melt adhesive and a preparation method thereof. The hot melt adhesiveis prepared from an isocyanate component and an isocyanate reactive component in parts by mass through reaction, wherein the isocyanate component is prepared from the following components in parts bymass: 15-35 parts of isocyanate; wherein the isocyanate reactive component comprises the following components in parts by mass: 30-55 parts of at least one polyether polyol; 15 to 50 parts by mass ofat least one polyester polyol; 0.01-10 parts by mass of at least one other hydroxyl compound containing a long-chain aliphatic hydrocarbon; and 0-3 parts by mass of hydroxyalkyl modified silicone oil.The reactive moisture-curable polyurethane hot melt adhesive disclosed by the invention has excellent moisture permeability, bonding strength, washing resistance and soft hand feeling; the preparation method has the advantages of simple steps, effective control of the side reaction, and improvement of the quality and the production stability of the final product.
Owner:WANHUA CHEM BEIJING +1

Dogwood extraction and its preparation method and usage

ActiveCN1569119AThe extraction process is simple and reasonableIncreased excretion rateOrganic active ingredientsSugar derivativesAlcoholElution
The invention provides a dogwood extraction and its preparation method and use thereof, wherein the preparation consists of extracting dogwood with alcohol, depression concentrating the raffinate to a finite density, collecting the deposition, decompressing and drying, obtaining extract A, passing through macroscopic adsorption resin column, removing foreign substance through washing, ethanol elution, collecting the eluent, decompressing and drying, obtaining extract B, mixing extract A and B homogeneously, thus obtaining the dogwood extract. The invention discloses its novel pharmaceutical use in preparing medicament for prevention and treatment of diabetic blood vessel pathological changes and for reducing blood sugar.
Owner:JIANGSU KANION PHARMA CO LTD

Intercultivation method for oreochromis mossambicus and white prawns

The invention relates to the technical field of aquaculture, and concretely relates to an intercultivation method for oreochromis mossambicus and white prawns. The method comprises the following steps: (1) pond treatment; (2) fry throwing and culturing; (3) cultivation management; and (4) water quality management. The invention aims to provide the intercultivation method for the oreochromis mossambicus and the white prawns, white prawn intercultivation in an oreochromis mossambicus pond can fully utilize cultivation water space, reduce prawn cultivation costs, effectively control prawn diseases and infection, and improve cultivation economic benefits.
Owner:桂平市金田镇旷福淮山种植专业合作社

System and method for disinfecting soilless culture medium

The invention relates to a system and method for disinfecting a soilless culture medium. The system for disinfecting the soilless culture medium comprises a drip irrigation system, a medium groove, a liquid storage pool, a liquid storage barrel and an electrolysis device. The drip irrigation system is arranged above the medium groove. The bottom of the medium groove is connected with the liquid storage pool through a liquid return pipe. Two submerged pumps are arranged at the bottom inside the liquid storage pool. One submerged pump is connected with the liquid storage barrel through a filter. The liquid storage barrel is further connected with an inlet of the electrolysis device through a booster pump. An anode liquid outlet of the electrolysis device is connected with the drip irrigation system through an anode terminal liquid return pipe and a cathode liquid outlet is connected with the liquid storage pool through a cathode terminal liquid return pipe. The invention further provides the method with the system for disinfecting the soilless culture medium adopted. According to the system for disinfecting the soilless culture medium, a disinfecting closed loop and a nutrient solution supply closed loop are obtained through a three-way valve, the automation degree is high, the energy consumption is small, pathogenic microorganism generated in the medium is thoroughly killed, occurrence and transmission of root transmission diseases can be effectively controlled, and residue and pollution are avoided.
Owner:BEIJING RES CENT OF INTELLIGENT EQUIP FOR AGRI

Multiple cropping cultivation method for eggplants

The invention relates to a multiple cropping cultivation method for eggplants. The method comprises the following steps: performing eggplant seedling raising and cultivation; after first eggplants are harvested in a fruit harvesting period, performing pruning, smearing wounds with pasty pesticide ointment to prevent disease infection, and topdressing water full of fertilizer, wherein the pesticide ointment is prepared from 10 to 50 grams of agricultural streptomycin, 10 to 30 grams of 75 percent chlorothalonil and 5 to 40 grams of 20 percent virus A wettable powder, and virus A wettable powder comprises moroxydine hydrochloride and copper acetate; when new side branches are sprouted, performing foliar fertilization, and producing and harvesting second-crop eggplants; then performing pruning, fertilization and the like to harvest all third-crop eggplants according to a cultivation method for the second-crop eggplants. According to the cultivation method, the disease resistance of the eggplants is improved, the planting and harvesting period of the eggplants is greatly prolonged, the yield is 2 to 3 times higher than that of a conventional planting method, the quality of each crop is more stable than that of the conventional planting method, and economic benefits are improved.
Owner:陕西秦灞现代农业开发有限公司

Special catalyst for hydrosilylation

The invention relates to a special catalyst for hydrosilylation, which comprises the following components in parts by weight: 1.5-2.5 parts of chloroplatinic acid and 0.8-1.2 parts of divinyl tetramethyl disiloxane. The invention relates to a catalyst which is used in the hydrosilylation of producing Gamma-chloropropyl trichlorosilane. The catalyst is a bran-novel chlorine platinum acid ligand, i.e. the divinyl tetramethyl disiloxane (tetramethyl divinyl disiloxane) which can catalyze the addition reaction at the room temperature, therefore, the energy used in the heating process is saved, the energy consumption is reduced and the equipment requirement is also reduced.
Owner:TIANJIN SHENGBIN CHEM

Pesticide composition for preventing and treating crop root nematodosis by foliage spray

The invention relates to a pesticide composition for preventing and treating crop root nematodosis by foliage spray. The pesticide composition comprises effective components including oxamyl and a plant resistance inducer, wherein the plant resistance inducer is a plant growth regulator with a resistance induction effect or any one or a mixture of two of active polysaccharides; the plant growth regulator comprises compound nitrophenolate and diethyl aminoethyl hexanoate; the active polysaccharides comprise chitosan oligosaccharide and glucal; the effective components comprise 0.05-90.0 parts by weight of the oxamyl and 0.05-90.0 parts by weight of the plant resistance inducer. According to the pesticide composition, the synergistic effects of nematode killing and resistance induction are utilized to control the generation and the development of the crop root nematodosis; the pesticide composition is conducted to crop roots by foliage absorption and nematodes are prevented from being invaded, parasitized and reproduced at the roots; meanwhile, damages to crops by toxic secreta of the nematodes are removed and the symptoms of etiolation, withering and the like are relieved; the formation of insect galls at the roots is relieved. The pesticide composition has an obvious preventing and treating effect on systemic translocation insects and leaf diseases of over-ground parts of the crops.
Owner:郭红甫

Heat-source-free slow-release polycarboxylic acid high-performance water reducing agent and preparation method thereof

The invention discloses a heat-source-free slow-release polycarboxylic acid high-performance water reducing agent, which comprises: 100 parts of isopentenyl polyoxyethylene ether, 6-10 parts of acrylic acid, 9-13 parts of hydroxyethyl acrylate, 5-9 parts of hydroxypropyl acrylate, 1-2 parts of 2-acrylamide-2-methyl propanesulfonic acid, 1-2 parts of sodium methylallyl sulfonate, 0.5-1.5 parts of sodium phosphite, 0.5-1 part of ammonium persulfate, 1-2 parts hydrogen peroxide, and 140-180 parts of water. The invention further discloses a preparation method of the heat-source-free slow-release polycarboxylic acid high-performance water reducing agent. According to the present invention, the preparation method has characteristics of simple production process and low cost, the obtained producthas advantages of high initial water reduction rate and significant sustained release effect, can maintain concrete at a loss-free state for 1-3 h, can effectively solve the problem of rapid slump loss of concrete, and can save the energy consumption.
Owner:四川恒泽建材有限公司

Strawberry planting method

The invention provides a strawberry planting method, and relates to the field of planting of strawberries. The planting method comprises seeding, transplanting and managing, wherein a biological source bactericide is sprayed during a strawberry growth process, the biological source bactericide comprises materials as follows: artemisia sieversiana, fructus cnidii, golden cypress, the root of Chinese pulsatilla, sealwort, garlic, semen cassiae, tobacco, soya bean lecithin and sorbitol fatty acid ester. The planting method provided by the invention can effectively control occurrence and spreading of diseases during a strawberry growth process, reduces phenomena of flower falling and fruit falling and a malformed fruit rate, improves quality and yield of the strawberries, and achieves the effect of planting without pollution.
Owner:覃淑兰

Fast detection method for high-resistance botrytis cinerea bacterial strains of carbendazim based on LAMP (loop-mediated isothermal amplification) technology

The invention discloses a fast detection method for high-resistance botrytis cinerea bacterial strains of carbendazim. The method can be used for performing dynamic monitoring and early warning on botrytis cinerea bacteria causing gray mold of crops for the resistance groups of the carbendazol. In the method disclosed by the invention, a molecular detection technology having the advantages of fastness, convenience, strong specificity, high sensitivity and low cost is established on the basis of a loop-mediated isothermal amplification technology (LAMP). The detection method comprises the following steps: designing 2 pairs of specific primers on beta tubulin of high-resistance bacterial strains of the botrytis cinerea bacteria for the carbendazol, performing LAMP amplification, and judging whether products are the high-resistance bacterial strains of the botrytis cinerea bacteria for the carbendazol or not according to the colors of reaction products. If the LAMP amplification products appear to be azure, electrophoresis patterns present ladder-shaped strips, and the product amplification is generated, the products are identified as the high-resistance bacterial strains of the botrytis cinerea bacteria for the carbendazol; if the LAMP amplification products present violet, the electrophoresis patterns do not have strips, and the product amplification is not generated, then the products are identified as non high-resistance bacterial strains of the botrytis cinerea bacteria for the carbendazol. The method is simple, convenient, fast, and low in cost and has important actual significance in the resistance risk assessment of the gray mold of the crops and reasonable medication.
Owner:NANJING AGRICULTURAL UNIVERSITY

Culturing method for blue crab egg-carrying crab phase

A culturing method for a blue crab egg-carrying crab phase relates to the field of aquiculture. A blue crab egg-carrying crab is placed in a crab apartment and cultured, meaning each blue crab egg-carrying crab is bred in a culturing box; water in the culturing box is filtered, ultraviolet-disinfected and protein-separation processed; in high temperature seasons, a crab apartment system can be placed in an air conditioning room; the culturing water temperature is kept at 22 DEG C to 26 DEG C and salinity is kept at 26 to 30; the water can be circulated for 8 times for each day; water exchanging amount for each day can be 15% to 25% of original water amount according to a volume percentage; one to two astartes are fed to each blue crab egg-carrying crab; food residuals and feces are cleaned for one time each day; a blue crab egg-carrying crab embryonic development process is determined according to embryonic development accumulated temperature and embryo heartbeat times; and to-be-hatched blue crab egg-carrying crabs are moved to a hatching barrel. The culturing method is simple and easy to operate.
Owner:XIAMEN UNIV

Multiple RT-PCR method for detecting five sugarcane viruses simultaneously as well as primer and kit thereof

The invention discloses a multiple RT-PCR detection primer group for detecting five kinds of sugarcane viruses simultaneously. The multiple RT-PCR detection primer group for detecting five kinds of sugarcane viruses simultaneously comprises six pairs of PCR primers which aim at five sugarcane viruses such as SCBV, SSV, SCMV, ScYLV and SrMV and one sugarcane reference gene GPADH correspondingly; the six pairs of PCR primers have base sequences as shown in a sequence table SEQ ID NO.1 to SEQ ID NO.12 correspondingly. Therefore, primer concentration and annealing temperature which influence multiple RT-PCR amplification are optimized by a multiple RT-PCR system and a corresponding RT-PCR detection method is established; and by the method, the five viruses for infecting the sugarcane can be detected simultaneously from sugarcane tissues rapidly, accurately, simply and conveniently, and important significance in screening healthy seedling mother plants, monitoring healthy seedling breeding,breeding for disease resistance and predicting field disease epidemic is achieved. In order to facilitate application of the multiple RT-PCR detection primer group, a corresponding kit is designed and assembled, so that popularization and promotion in grass roots are facilitated.
Owner:GUANGXI UNIV

Method used for treating urban river and lake algal bloom

The invention discloses a method used for treating urban river and lake algal bloom, and especially relates to a formula of an algae inhibitor, and an application method thereof. The algae inhibitor is a water solution of isothiazolone, dichloroisocyanurate or trichloroisocyanurate, polyhexamethyleneguanidine chloride, and inorganic salt. The mass ratio of the materials is 0.5%-5%:0.1%-2.5%:0.05%-1%:0.0001%-0.005%. During a flood season, the algae inhibitor is added to a water body requiring treatment every 3-15 days; when rainfalls such as a moderate rain or a more severe rain occur, the addition frequency is appropriately increased. The dosage amount of the algae inhibitor is 0.002-0.05kg / m<3> each time. With the algae inhibitor and the application method provided by the invention, algae amount in water bodies can be effectively reduced, and occurrence of algal bloom can be effectively avoided.
Owner:和濡水尚科技(北京)有限公司 +2

Mixed breeding method of carassius auratus gibelio and penaeus vannamei boone

The invention relates to a mixed breeding method of carassius auratus gibelio and penaeus vannamei boone. The method comprises the steps of pond preparation, fingerling and young shrimp putting, bait feeding, breeding management and capturing. The mixed breeding method of the carassius auratus gibelio and the penaeus vannamei boone is simple, the breeding cost is low, the breeding yield can be greatly increased, the total revenue of breeding of the carassius auratus gibelio and the penaeus vannamei boone is improved, and a good application prospect is achieved.
Owner:INST OF OCEANOLOGY & MARINE FISHERIES JIANGSU

Pigeon house electrical feeding disinfection immunization all-in-one machine

The invention relates to an all-in-one machine for dovecote electric feeding, disinfection and immunity. The all-in-one machine includes an electric traveling mechanism, a feeding mechanism, a disinfection mechanism and an immune mechanism. The immune mechanism includes a control switch three, a connecting pipe, a vaccine dilution bottle, a spray head and a vaccine spray immunity box. The vaccine spray immunity The box is in the middle and upper part of the frame and is located above the water tank. The connecting pipe is connected to the vaccine dilution bottle, and a control switch 3 is set on the connecting pipe. The vaccine dilution bottle is connected to a water pipe, and a nozzle is arranged on the water pipe. The vaccine Both the dilution bottle and the spray head are arranged in the vaccine spray immunization box. The invention has the advantages of simple structure, convenient cleaning, labor saving and material saving, and various uses.
Owner:JIANGSU INST OF POULTRY SCI

Ultrasonic atomizing device

The invention discloses an ultrasonic atomizing device. The ultrasonic atomizing device comprises an atomizing box, an atomizing chamber, a mist discharging chamber, a mixing chamber, a heating chamber, an ultrasonic transducer, an air pump, a mist discharging spray head, a fan, a heater and an exhaust nozzle, wherein the atomizing chamber, the mist discharging chamber, the mixing chamber and theheating chamber are sequentially arranged in the atomizing box from bottom to top, the ultrasonic transducer is arranged in the atomizing chamber, the air pump is arranged in the mist discharging chamber, the mist discharging spray head is connected with the air pump, the fan and the heater are arranged in the heating chamber, and the exhaust nozzle is used for exhausting hot air in the heating chamber into the mixing chamber. According to the ultrasonic atomizing device, liquid can be atomized into smaller liquid drops through combination of primary ultrasonic and secondary hot air so that the atomized liquid drops can suspend in the air for a long time, and a better use effect is achieved; mist and hot air can be sufficiently mixed by arranging a flow guide mixing assembly, the heating effect of the hot air on the mist can be improved, and droplets with smaller diameters can be formed by the mist; and according to the ultrasonic atomizing device, by arranging an automatic liquid supplementing assembly, solution supplementing can be automatically carried out on the atomizing chamber, so that efficient operation of the ultrasonic atomizing device is maintained.
Owner:SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI

Bacterial synthetic community and application thereof in preparation of biological control agent for controlling postharvest diseases of citrus fruits

PendingCN112695002AThe effect of early onset is obviousThe effect of disease control is weakenedFruit and vegetables preservationBacteriaBiotechnologyCitrus volkameriana
The invention discloses a bacterial synthetic community, which is composed of eight bacteria, namely Acinetobacter lwoffii, Bacillus amyloliquefaciens, Curptobacterium luteum, Curptobacterium sp., Methylobacterium platani, Pantoea sp., Pseudomonas parasulva and Pseudomonas sp., compared with a single bacterial strain, the bacterial synthetic community can better control the occurrence and progress of citrus green mold and sour rot, can more enduringly control diseases, can be used for preparing a biological control agent for controlling postharvest diseases of citrus fruits, has good application potential. A new thought and method are provided for controlling the postharvest diseases of the citrus fruits, and the application range of the bacterial synthetic community is widened.
Owner:SOUTHWEST UNIVERSITY

Application of litsea cubeba extract as silage corn anti-mildew additive

PendingCN111513233AHigh protein contentIncrease the total amount of amino acidsFood preservationAnimal fodder preservationBiotechnologyNutrition
The invention discloses an application of a litsea cubeba extract as a silage corn anti-mildew additive. The litsea cubeba extract is an extract obtained from litsea cubeba fruits by adopting a Soxhlet extraction method, and the litsea cubeba extract is directly mixed with silage corn. According to the application, the litsea cubeba extract is added into the silage corn, so that moulds of four mould genera of fusarium, aspergillus flavus, penicillium and rhizopus can be effectively inhibited, and the occurrence of moulds in the silage corn is effectively controlled; and the litsea cubeba extract can also significantly improve the protein content, the total amino acid content and the total fatty acid content in the silage corn and improve the nutritional quality of the silage corn, and hasa high application prospect.
Owner:INNER MONGOLIA AUTONOMOUS REGION ACAD OF AGRI & ANIMAL HUSBANDRY SCI

Magnaporthe oryzae avirulence gene AvrPib specific molecular marker, and method and application thereof

The invention relates to the technical field of gene engineering, and particularly discloses a Magnaporthe oryzae avirulence gene AvrPib specific molecular marker, and a method and application thereof. The sequences of the molecular marker are as shown in SEQ ID NO:1-4. By using the molecular marker, a Magnaporthe oryzae natural population pathogenicity variation molecule detection system can be established, and the distribution of the gene in natural population in the field and the dynamic change rule thereof can be known and mastered, thereby guiding the breeding for resistance to rice blast and the rational distribution and alternation of species in various regions. Thus, the breeding efficiency is improved, rice blast can be controlled more effectively, and a new strategy for comprehensively controlling rice blast can be designed accordingly.
Owner:SOUTH CHINA AGRI UNIV

Mixed feed for preventing and curing laying hen contagious viral diseases

The invention relates to the technical field of mixed chicken feed for laying hens, in particular to mixed feed for preventing and curing laying hen contagious viral diseases, prepared from the following materials in parts by weight: 5 to 8 parts of sweet potato leaf, 5 to 8 parts of erigeron breviscapus, 10 to 15 parts of angelica sinensis, 3 to 6 parts of gynostemma pentaphyllum, 5 to 8 parts of medlar, 1 to 2 parts of gastrodia elata, 1 to 2 parts of radix cynanchi bungei, 3 to 6 parts of liquorice, 5 to 8 parts of houttuynia cordata, 10 to 15 parts of isatis root, 5 to 8 parts of folium isatidis, 5 to 8 parts of fructus forsythiae, 1 to 2 parts of aerba lupulin, 3 to 6 parts of semen cuscutae, 1 to 2 parts of selfheal, 3 to 6 parts of morinda officinalis, 3 to 6 parts of eucommia ulmoides, 3 to 6 parts of Chinese yam, 1 to 2 parts of dangshen, 30 to 50 parts of yellow maize, 5 to 8 parts of middlings, 10 to 15 parts of alfalfa powder, 10 to 15 parts of fried soybean, 10 to 15 parts of peanut meal, 1 to 2 parts of calcium lactate, 0.01 to 0.5 parts of probiotics, 0.01 to 0.5 parts of choline chloride, 0.01 to 0.05 parts of Jinxingweita, 0.01 to 0.2 parts of microelement, 0.01 to 1 part of salt and 1 to 5 parts of shell powder. A laying hen using the mixed feed provided by the invention for prevention and curing by traditional Chinese medicine has an illness catching rate of only 0.3 percent; after a sick hen is fed with the mixed feed, the death and culling rate is reduced by over 10 percent, and the veterinary drug residues of eggs laid by the recovered hen do not exceed standards.
Owner:峨眉山市全林农业科技有限公司

A kind of multi-crop cultivation method of eggplant

The invention relates to a multiple cropping cultivation method for eggplants. The method comprises the following steps: performing eggplant seedling raising and cultivation; after first eggplants are harvested in a fruit harvesting period, performing pruning, smearing wounds with pasty pesticide ointment to prevent disease infection, and topdressing water full of fertilizer, wherein the pesticide ointment is prepared from 10 to 50 grams of agricultural streptomycin, 10 to 30 grams of 75 percent chlorothalonil and 5 to 40 grams of 20 percent virus A wettable powder, and virus A wettable powder comprises moroxydine hydrochloride and copper acetate; when new side branches are sprouted, performing foliar fertilization, and producing and harvesting second-crop eggplants; then performing pruning, fertilization and the like to harvest all third-crop eggplants according to a cultivation method for the second-crop eggplants. According to the cultivation method, the disease resistance of the eggplants is improved, the planting and harvesting period of the eggplants is greatly prolonged, the yield is 2 to 3 times higher than that of a conventional planting method, the quality of each crop is more stable than that of the conventional planting method, and economic benefits are improved.
Owner:陕西秦灞现代农业开发有限公司

Temperature-increasing system increasing indoor temperature of edible mushroom planting room through wind energy

InactiveCN103609368AImprove supplemental heating efficiencyPest controlClimate change adaptationCultivating equipmentsHeat sinkElectric energy
The invention discloses a temperature-increasing system increasing the indoor temperature of an edible mushroom planting room through wind energy. The system comprises a wind power generation set, a heater and heat dissipation pieces, the wind power generation set is connected with the heater, the heater is connected with the heat dissipation pieces, and the heat dissipation pieces are arranged on the periphery inside a planting room. According to the system, wind energy is converted into electric energy through the wind power generation set, the electric energy is further used for heating of the heater, thee indoor temperature of the planting room is increased through the heat dissipation pieces, the supplementing heat supply efficiency can be effectively improved, occurrences of pests and diseases are controlled, and economic benefits are greatly improved.
Owner:洛宁县志博农业新技术推广有限公司

Multiple detection method and kit for simultaneously detecting three sweet potato viruses

The invention relates to the technical field of sweet potato virus detection, in particular to a multiple detection method and a kit for simultaneously detecting three sweet potato viruses. The kit comprises a 10 * reaction buffer solution, Taq enzyme, upstream primers of the three sweet potato viruses, downstream primers of the three sweet potato viruses and a positive standard substance. The three sweet potato viruses are a sweet potato chlorotic dwarf virus, a sweet potato pinniform mottle virus and a sweet potato latent virus respectively; the detection method comprises the following steps: (1) extracting total RNA (Ribonucleic Acid) of a sample to be detected, and carrying out reverse transcription to obtain cDNA; (2) PCR amplification; (3) taking 5 mu L of the PCR amplification product in the step (2), and performing electrophoresis on 2% agarose gel containing 5 mu g / ml of ethidium olfactory (EB); (4) result judgment: PCR target amplified fragments are respectively 150bp, 280bp and 480bp; according to the invention, three kinds of sweet potato viruses can be simultaneously detected through one PCR reaction, the operation frequency is reduced, cross contamination is avoided, the usage amount of reagents, primers, templates, consumables and the like is also reduced, and time, labor and cost are saved.
Owner:HENAN UNIV OF SCI & TECH +1

A primer set, kit and method for detecting pathogenic variants of avirulent gene avrpi9 of oryzae oryzae

The invention discloses a primer set, a kit and a method for detecting the pathogenic variation of the rice blast fungus avirulence gene AvrPi9. SNP changes in the avirulent gene AvrPi9 of Magnaporthe grisea lead to premature termination of the coding of the AvrPi9 gene and transformation into a virulent gene. The primer set includes two pairs of primers, the first pair of primers is used to amplify the AvrPi9 gene fragment; the second pair of primers is used to identify whether there is a SNP that causes AvrPi9 to transform into a toxic gene after mutation. The invention provides two pairs of molecular markers of the avirulent gene AvrPi9 of Magnaporthe grisea. The molecular markers can be used to quickly establish a molecular detection system for the pathogenic variation of AvrPi9 in the natural population of Magnaporthe grisea. The distribution and dynamic changes in the population are used to guide rice blast resistance breeding in various regions and the rational distribution and rotation of varieties, so as to improve breeding efficiency and control the occurrence of rice blast more effectively, so as to design rice blast comprehensive new strategies for prevention.
Owner:CHINA NAT RICE RES INST

Elbow type inlet passage with arc-shaped spreader plate

The invention provides an elbow type inlet passage with an arc-shaped spreader plate, and belongs to the technical field of water conservancy projects. The elbow type inlet passage comprises a linear gradually reducing section, an elbow gradually reducing section, a straight cone section and a separating pier, and is characterized in that the arc-shaped spreader plate is arranged in the elbow gradually reducing section, the longitudinal section of the arc-shaped spreader plate is in an arc shape, the central line of the arc-shaped spreader plate and the central line of the elbow gradually reducing section are consistent in shape. When water flow flows out of the linear gradually reducing section of the elbow type inlet passage, the water flow is uniform in flow speed distribution, but when the water flow flows in the elbow gradually reducing section, the mainstream of the water flow deviates to the inner side of the elbow type inlet passage and the flow speed of the inner side is higher than that of the outer side. Then, when the water flow passes through the elbow gradually reducing section of the arc-shaped spreader plate, the flowing and separating of the elbow gradually reducing section are reasonably controlled, the water flow of the inlet passage is more smooth in flowing, and the flow speed uniformity of the elbow gradually reducing section and the flow speed distribution of the outlet of the inlet passage are improved.
Owner:扬州市勘测设计研究院有限公司 +1

A plant cultivation method

The invention relates to the field of plant cultivation, in particular to a plant cultivation method. It includes: transplanting the substrate with seedling plants to the planting board; placing the planting board on the planting groove, and the root system of the plant hangs into the planting groove through the cultivation hole on the planting board; Airtight treatment of the plant root space; wherein, sealing the pores between the substrate and the planting plate, sealing the pores at the junction of the planting groove and the planting plate; passing a certain concentration of oxygen into the planting groove ; Adopting a suitable culture method to cultivate the plant. According to the plant cultivation method of the present invention, the entire root system of the plant is placed in a closed high-humidity oxygen-enriched microenvironment, so as to promote the growth and development of the root system and improve the ability of the entire device to resist water cut-off.
Owner:4D BIOS INC

Primer group, kit and method for detecting pathogenic variation of magnaporthe grisea avirulent gene AvrPi9

The invention discloses a primer group, a kit and a method for detecting pathogenic variation of a magnaporthe grisea avirulence gene AvrPi9. The magnaporthe grisea avirulence gene AvrPi9 is subjected to SNP change, so that AvrPi9 gene coding is terminated in advance, and the gene is converted into a toxic gene. The primer group comprises two pairs of primers, wherein the first pair of primers is used for amplifying an AvrPi9 gene segment; and the second pair of primers is used for identifying whether SNP causing AvrPi9 to be converted into a toxic gene after mutation exists or not. The invention provides two pairs of molecular markers of magnaporthe oryzae non-toxic genes AvrPi9, a molecular detection system of AvrPi9 pathogenic variation in a magnaporthe oryzae natural population can be quickly established by utilizing the molecular markers, and the distribution of the genes in the field natural population and the dynamic change rule of the genes are understood and mastered. Therefore, rice blast disease-resistant breeding and reasonable variety layout and rotation in each region are guided, so that the breeding efficiency is improved, rice blast is more effectively controlled, and a new strategy for comprehensively preventing and treating rice blast is designed.
Owner:CHINA NAT RICE RES INST

Green cultivation instrument for livestock and poultry

The invention provides a green culturing instrument for livestock and poultry, which comprises four parts: an air processing unit, a water processing unit, a casing and a control part. The air processing unit comprises three parts: a fan (1), a fan drum (2) and a plasma generating device. The fan (1) is connected with the fan drum (2) which is connected with an open generating tube of the plasma generating device. The water processing unit comprises three parts: an air pump (10), the plasma generating device and an efflux nozzle (13). The air pump (10) is connected with the plasma generating tube of the plasma generating device, and the generating tube is connected with the efflux nozzle (13). Pathogenic microorganisms in atmosphere and water are thoroughly and rapidly killed. The antibody level of livestock and poultry is improved by over three times. The immunity is enhanced. Body immune cells are activated. The disease resistance of the livestock and poultry is improved. Disinfection with the presence of livestock and poultry can be carried out at any time without dead corners left and secondary pollutions. The occurrence and spread of heavy diseases of livestock and poultry can be controlled effectively.
Owner:XIAN DERUN BIO TECH

Bacillus pumilus strain and micro-ecological preparation and feed thereof

The invention belongs to the technical field of biologics, and discloses bacillus pumilus capable of inhibiting streptococcus agalactiae and application thereof. The bacillus pumilus has the advantages that the screened bacillus pumilus metabolite can be used for obviously inhibiting the metabolism and growth of the streptococcus agalactiae, the number of streptococcus agalactiae in the tlapia mossambica culture environment is reduced, and the occurrence of streptococcicosis is inhibited; the bacillus pumilus is subject to multiple times of strain selection and breeding, and can be performed with liquid deep fermenting, so as to prepare water preparation products and powder preparation products; the powder preparation products are prepared into a tlapia mossambica particle feed according to a certain ratio, and then the tlapia mossambica particle feed can be applied into aquaculture.
Owner:TIANJIN CHANGNONG TECH +2
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products