The invention discloses a detection kit for Thrombophilia-
related gene mutation. The detection kit comprises ddH2O, 10*buffer (Takara), 2*GC buffer I, dNTP, MgCl2, HSTaq,
multiplex PCR primer mixed liquid, Illumina I5 / I7 primer series reagents. The detection kit has the advantages that the detection kit uses the
multiplex PCR enriching next generation sequencing technology to comprehensively and systematically detect the
point mutation of all exons and control regions of 18 genes which include PROC, PROS1, THBD, PROCR, F5, AT3, HCF2, F2, F7, F8, F9, F10, F11, F12, PLG, ADAMTSl3, HRG and TFPI and performs detection aiming at the specific polymorphic sites of three genes which include PLAT, FGG and HABP2; the detection kit is used for biological researches and molecularly diagnosing the Thrombophilia caused by the defects of the genes above; the detection kit is wide in detection range, high in
throughput, fast and accurate in detection method, high in detection accuracy, low in cost, and the like.