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Gene NS1 (Structural Protein) defective respiratory syncytial virus and application thereof

A technology of syncytial virus and defective virus, applied in the direction of virus/bacteriophage, disease, drug combination, etc., can solve the problem that there is no respiratory syncytial virus to treat breast cancer, and achieve the effect of treating breast cancer

Inactive Publication Date: 2011-04-20
张卫东 +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, there are no reports of the use of genetically engineered respiratory syncytial virus to treat breast cancer

Method used

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  • Gene NS1 (Structural Protein) defective respiratory syncytial virus and application thereof
  • Gene NS1 (Structural Protein) defective respiratory syncytial virus and application thereof
  • Gene NS1 (Structural Protein) defective respiratory syncytial virus and application thereof

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Embodiment Construction

[0028] In order to confirm whether the generated virus ΔNS1 RSV lacks the NS1 gene, the inventors infected Vero cells (a kind of IFN-β gene-deficient cells) with wild wt RSV and ΔNS1 RSV respectively, and the NS1 protein was detected by Western blotting, Figure 2A It was shown that NS1 protein was only present in wild RSV, but not in ΔNS1 RSV. This result indicated that ΔNS1 RSV lacked NS1 gene.

[0029] In order to verify whether ΔNS1 RSV can selectively kill human breast cancer cells, MDA-MB-231 breast cancer cell line and normal cell line CCD-1059SK (human normal breast fibroblasts) were cultured under the guidance of ATCC (American Type Culture Collection), At the same time, wild (wt) and genetically engineered ΔNS1RSV viruses were infected, and their morphological changes and virus replication were detected. Figure 2B It shows that ΔNS1 RSV selectively induces MDA-MB-231 breast cancer cell lesions. After 24 hours of infection, the titer of ΔNS1 RSV in cancer cells is si...

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Abstract

The invention relates to a gene NS1 (Structural Protein) defective respiratory syncytial virus and application thereof. The gene NS1 defective respiratory syncytial virus is synthesized by Vero cells, and the Vero cells are formed through plasmid cotransfection of genes carrying NS1-defective virus cDNA, viruses N, M2-1, P and L. In the gene NS1 defective respiratory syncytial virus, the gene NS1 in the respiratory syncytial virus is deleted by utilizing a reverse gene strategy. The gene NS1 defective respiratory syncytial virus is applied to treating breast cancer and kills breast cancer cells selectively.

Description

technical field [0001] The invention relates to respiratory syncytial virus (RSV), specifically a gene NS1-deficient respiratory syncytial virus (ΔNS1 RSV) and application thereof. Background technique [0002] Breast cancer is one of the most common malignant tumors in women. According to statistics, the incidence rate accounts for 7-10% of various malignant tumors in the whole body. Its incidence is often related to heredity, and the incidence rate is higher in women between the ages of 40 and 60 and before and after menopause. Malignant tumors usually occurring in the glandular epithelium of the breast. It is one of the most common malignant tumors that seriously affects women's physical and mental health and even threatens their lives. Breast cancer is common in women. It is estimated that there are more than one million new cases every year in the world. In 2009 alone, 192,370 people were newly diagnosed with breast cancer in the United States, and about 40,170 people...

Claims

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Application Information

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IPC IPC(8): C12N7/00A61K39/155A61P15/14A61P35/00C12R1/93
Inventor 张卫东蒋礼先高利卫
Owner 张卫东
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