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Bacillus pumilus and application thereof for killing bursaphelenchus xylophilus with poison

A technology for Bacillus pumilus and pine wood nematodes, which is applied in the directions of nematicides, applications, bacteria, etc., can solve problems such as no Bacillus pumilus plant nematodes, and achieve good development and application prospects, simple culture conditions, and easy industrial production. Effect

Active Publication Date: 2010-12-01
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the control of plant nematodes by Bacillus pumilus at home and abroad

Method used

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  • Bacillus pumilus and application thereof for killing bursaphelenchus xylophilus with poison
  • Bacillus pumilus and application thereof for killing bursaphelenchus xylophilus with poison
  • Bacillus pumilus and application thereof for killing bursaphelenchus xylophilus with poison

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Test tube culture: The medium formula is: every 1000mL of distilled water contains 3g of beef extract, 10g of peptone, 15g of agar, pH 7.2-7.4, inoculate Bacillus pumilus JK-SD001 on the medium, at 25℃~38℃ Cultivate for 24 hours to obtain test tube seeds;

[0026] Preparation of microbial nematocide: The formula of the liquid medium is: every 1000mL of distilled water contains 3g of beef extract, 10g of peptone, pH7.2-7.4, inoculate the Bacillus pumilus JK-SD001 test tube on 50mL of the liquid medium, 25°C And under the condition of no light with 120r / min rotating speed shaking culture 24h, obtain liquid seed; Liquid seed is inoculated in the liquid culture medium that liquid filling ratio is 1: 2, inoculation amount 2% (v / v), 37 ℃, 120r / min, shaking and fermenting for 96 hours without light. The sterile filtrate obtained by passing the fermentation broth through a filter with a pore size of 0.22 microns.

Embodiment 2

[0028] The liquid seed is prepared according to the method of Example 1, and the liquid seed is inoculated into a liquid medium with a liquid filling ratio of 1:2, the inoculum amount is 3% (v / v), 35° C., 100 r / min, and shakes without light Ferment for 90 hours. The sterile filtrate obtained by passing the fermentation broth through a filter with a pore size of 0.22 microns.

Embodiment 3

[0030] The liquid seed is prepared according to the method of Example 1, and the liquid seed is inoculated into a liquid medium with a liquid filling ratio of 1:2, the inoculum amount is 5% (v / v), 38° C., 200 r / min, and shakes without light Ferment for 100 hours. The sterile filtrate obtained by passing the fermentation broth through a filter with a pore size of 0.22 microns.

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Abstract

The invention discloses a bacillus pumilus JK-SD001. Subclass thereof is named as Bacilluspumilus and is preserved in China Center for Type Culture Collection CCTCC, preservation number thereof is M 209095, and preservation date is May 4th, 2009. The invention also discloses application of bacillus pumilus for killing bursaphelenchus xylophilus with poison. The invention also provides a micro organism bursaphelenchus xylophilus killing agent, obtained by the way that JK-SD001 is inoculated to a culture medium to be fermented and cultured and filtering is carried out by virtue of a filter with the aperture of 0.22Mum. The micro organism bursaphelenchus xylophilus killing agent of the invention has higher toxicity on bursaphelenchus xylophilus (B. xylophilus), and culture condition is simple and preservation is easy, thus being easy for industrialized production and having good application prospect.

Description

technical field [0001] The invention belongs to the technical field of microbial pesticides, and in particular relates to a bacillus pumilus and its application in poisoning and killing pine wood nematodes. Background technique [0002] At present, the situation of the spread of the epidemic is still very serious. Pine wood nematode disease not only affects forestry production, but also brings immeasurable losses to landscaping, tourism, and people's lives. The prevention and control of this disease has become a hot and difficult research topic at home and abroad. [0003] At present, chemical control is mostly used for the prevention and control of pine wood nematode disease, but the application of chemical agents is gradually limited due to problems such as high toxicity and environmental pollution. For example, the widely used chemical nematicides, fonsofos and phamiphos, have been banned due to teratogenicity, carcinogenicity, and sperm reduction. Most of the existing ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P5/00C12R1/07
Inventor 吴小芹邓海娟任嘉红叶建仁
Owner NANJING FORESTRY UNIV
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