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Bacillus amyloliquefaciens subsp.plantarum B232 and application thereof

A technology of amylolytic spores and bacillus plants, applied in the fields of application, plant growth regulator, botany equipment and methods, etc., which can solve problems such as death and no big trees seen

Inactive Publication Date: 2014-12-10
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the increase of poplar seedlings and afforestation area, it has occurred to varying degrees in poplar forests in Jiangsu and other places, and it has become more and more serious, but no cases of death of large trees have been seen.

Method used

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  • Bacillus amyloliquefaciens subsp.plantarum B232 and application thereof
  • Bacillus amyloliquefaciens subsp.plantarum B232 and application thereof
  • Bacillus amyloliquefaciens subsp.plantarum B232 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0037] Experimental Example 1: Bacillus amyloliquefaciens subsp.plantarum B232 is effective against poplar large spotted canker (Dothichiza populea), Dendrobium officinale gray mold (Botrytis cinerea), Dendrobium officinale white spot disease (Athelia rolfsii), Bacteriostatic activity of strawberry root rot (Fusarium oxsporum) and wheat head blight (Fusarium asiaticum) on plates.

[0038] The Bacillus amyloliquefaciens subsp.plantarum B232 strain activated on the LB slant was streaked and inoculated on one side of the PDA plate, and then the poplar canker sore, Dendrobium officinale gray mold, and Dendrobium officinale , Strawberry root rot and wheat scab 90mm PDA plate culture with a puncher with a diameter of 5mm to get the same quality and the same amount of bacteria block, inoculated on the other side of the plate. Cultivate at 30±1°C for 14 days and 28 days to observe the antibacterial ability, and record its width (as shown in Table 1). The experimental effect is as follow...

experiment example 2

[0048] Experimental Example 2: Bacillus amyloliquefaciens subsp.plantarum B232 metabolites are effective against poplar large spotted canker (Dothichiza populea), Dendrobium officinale gray mold (Botrytiscinerea), Dendrobium officinale white spot disease (Athelia rolfsii ), Strawberry root rot (Fusarium oxsporum), wheat head blight (Fusarium asiaticum) plate antibacterial effect.

[0049] The fermentation broth of Bacillus amyloliquefaciens subsp.plantarum (Bacillus amyloliquefaciens subsp.plantarum) B232 cultivated for 3 days was filtered with a bacterial filter, and the filter membrane pore size of the bacterial filter was 0.25 μm; the 90 mm PDA plate culture of five kinds of plant pathogenic fungi was used Use a hole puncher with a diameter of 5mm to get the same quality and the same amount of bacterial blocks, and inoculate them on one side of the PDA plate; Take out the block; then connect 100 μL of the sterile filtrate of the filtered Bacillus amyloliquefaciens plant sub...

experiment example 3

[0060] Experimental Example 3: A test to verify whether Bacillus amyloliquefaciens subsp. plantarum B232 has an inhibitory effect on poplar canker bacterium.

[0061] 1. Experimental equipment

[0062] Ultra-clean workbench, PRX-250A intelligent artificial climate box, Peiying brand DHZ-DA TCYQ constant temperature oscillator.

[0063] 2. Experimental principle

[0064] After inoculating the poplar leaf canker fungus on the poplar trunk, brush Bacillus amyloliquefaciens subsp.plantarum B232 culture solution on the surface of the canker lesion, and B232 can pass through the wound and the bark surface The tiny cracks in the canker lesions penetrate into the canker lesions to continue to grow and reproduce, and produce metabolites, which can effectively control the growth of Dothichiza populea.

[0065] 3. Experimental method:

[0066] 1) Cultivation of Bacillus amyloliquefaciens subsp. plantarum B232 bacterial liquid: Inoculate Bacillus amyloliquefaciens subsp. plantarum B232...

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Abstract

The invention discloses bacillus amyloliquefaciens subsp.plantarum which is classified and named as bacillus amyloliquefaciens subsp.plantarum B232 and preserved in the China General Microbiological Culture Collection Center (CGMCC) of the China Committee for Culture Collection of Microorganisms (CCCCM) on June 9th, 2014, and has the preservation number of CGMCCNo.9279. The bacillus amyloliquefaciens subsp.plantarum B232 is mainly used for carrying out biological prevention and treatment on Dothichiza populea Sacc.et Briard, and has good functions of inhibiting Dothichiza populea Sacc.et Briard germs, dendrobium officinale botrytis and other various pathogenic fungi. The bacillus amyloliquefaciens subsp.plantarum B232 taken as an antagonistic bacterium also has good effects of preventing and treating other plant diseases. The bacillus amyloliquefaciens subsp.plantarum B232 has the advantages of being rapid in propagation speed, easy to operate, convenient in production application, strong in stress resistance and the like, and can be artificially cultured. The bacillus amyloliquefaciens subsp.plantarum B232 requires a simple culture condition and is easy to store and favorable for industrial production. Thus, the bacillus amyloliquefaciens subsp.plantarum B232 has a good development and application prospect.

Description

technical field [0001] The invention belongs to the field of plant protection, and in particular relates to Bacillus amyloliquefaciens subsp. plantarum B232 and its application in preventing and treating poplar canker. Background technique [0002] With the general concern of the public on the safety of chemical pesticides in the environment, it has prompted people to seek safe and effective new ways to control plant diseases, and the use of beneficial microorganisms in nature to control plant diseases has attracted increasing attention. It introduces exogenous antagonistic bacteria beneficial to plants into the broken plant micro-ecosystem, and promotes the natural beneficial microbial population to reproduce and grow continuously by regulating the micro-ecological environment, and exhibits biological control activity, so as to prevent plant diseases. Purpose. [0003] There are many types of potential microbial resources in nature, and they have obvious advantages in the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/07
Inventor 赵桂华赵楠杨鹤同
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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