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Bacillus subtilis 203 and applications thereof

A technology for Bacillus subtilis and banana Fusarium wilt, applied in the application, bacteria, fungicides and other directions, can solve problems such as weak technology and blank, and achieve the effects of fast reproduction, easy preservation, and broad antibacterial spectrum.

Inactive Publication Date: 2009-10-07
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Isolation and screening from banana plants to obtain biocontrol bacterial strains with antagonistic effects on Fusarium wilt of banana is of great significance to the control of Fusarium wilt of banana, but the relevant technology is still weak or even blank

Method used

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  • Bacillus subtilis 203 and applications thereof
  • Bacillus subtilis 203 and applications thereof
  • Bacillus subtilis 203 and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Plate Antagonism Experiment of Bacillus subtilis 203 to No. 4 Race FOC4 of Banana Fusarium wilt

[0025] Bacillus subtilis 203 was obtained by face-off screening on a plate, and the screening method belongs to a conventional method in the art. In the center of the potato sucrose medium PDA plate, insert a piece of FOC4 mycelium block of Fusarium wilt No. 4 race with a diameter of 5 mm, and then put a sterilized filter paper sheet with a diameter of 5 mm on the triangle of the equidistant FOC4 mycelium block, each piece Insert 10 μL of Bacillus subtilis 203 culture solution on the filter paper, take the plate without Bacillus licheniformis 202 culture solution as the control, repeat three times, culture in the incubator at 25-28°C for 5-7 days, measure the diameter of the inhibition zone, and use the attached figure 1 It can be seen that Bacillus subtilis 203 significantly inhibits the growth of Fusarium wilt.

Embodiment 2

[0026] Example 2 Bacillus subtilis 203 prevents banana plant main root and vascular bundle from browning or rot experiment

[0027] Select Brazilian banana seedlings with a plant height of about 15 cm, and carry out 3 different treatments: (1) clean water healthy plant control: the roots of banana seedlings are soaked in sterile water and then directly transplanted in a nutrient bowl to grow; (2) the roots of banana seedlings are soaked in sterile water. After 30 minutes in the Bacillus subtilis 203 culture solution, transplant it into the nutrient bowl, and then pour 20mL of FOC4 spore suspension into the nutrient bowl soil; (3) Incidence control: root irrigation of banana seedlings inoculated with 20mL of FOC4 spores Suspension, not inoculated with Bacillus subtilis 203 culture solution. For the above treatments, there were 20 plants in each treatment group. Afterwards, culture at 26°C, investigate the incidence rate and disease index at 10, 20, and 30 days after inoculatio...

Embodiment 3

[0028] Example 3 Antagonism of Bacillus subtilis 203 to various pathogenic bacteria

[0029] The antagonism of Bacillus subtilis 203 to 7 pathogenic bacteria including tomato fusarium wilt, Curvularia cornis leaf spot, glyosporium anthracnose, cotton wilt, cotton Verticillium wilt, wheat scab, and watermelon wilt was tested by plate confrontation method Effect, insert a target mycelium block with a diameter of 5mm in the center of the PDA plate, and then put a sterilized filter paper sheet with a diameter of 5mm on the triangle of the equidistant FOC4 mycelium block, and insert Bacillus subtilis 203 on each filter paper sheet for cultivation 10 μL of liquid solution, and a plate without Bacillus licheniformis 202 culture solution as a control, cultivated in a 28°C incubator for 5 to 7 days, and measured the diameter of the inhibition zone. Bacillus subtilis 203 has a wide antibacterial spectrum and has antagonistic effects on various pathogenic fungi such as Fusarium wilt of c...

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Abstract

The invention discloses a bacillus subtilis 203 which is preserved by China Center for Type Culture Collection (CCTCC) of Wuhan University at Luojiashan mountain of Wuchang of Wuhan city in Hubei province, with the preservation number of CCTCC No: M207094. The invention also discloses a preparation method and applications thereof. The bacillus subtilis 203 is obtained by slab antagonism and pot experiment screening, can effectively inhibit the growth of fusarium axysporum of banana, has wide antimicrobial spectrum, has antagonistic effect to various pathogenic fungi such as fusarium vasinfectum, verticillium dahliae kleb, fusarium axysporum of watermelon, fusarium axysporum of tomato, curvularia lunata or colletotrichum musae and the like, and has the advantages of good environment safety performance and excellent development and application prospects.

Description

technical field [0001] The invention relates to the field of plant protection, in particular to an endogenous biocontrol bacterial strain, in particular to a bacillus subtilis 203 for efficiently preventing and controlling banana wilt and its application. Background technique [0002] Banana Fusarium wilt is a devastating disease caused by the infection of Fusarium oxysporum f.sp.cubense, and it is a devastating soil-borne disease of banana production. The pathogen invades from the injured rhizome, spreads to the pseudostem and leaves through the vascular bundle, and blocks the xylem vessel until the plant withers and dies. Fusarium wilt of banana is a soil-inhabiting fungus, and its chlamydospores can survive in the soil for 8 to 10 years. Banana Fusarium wilt is distributed in many counties and cities in the banana production area of ​​the Pearl River Delta, and it has developed rapidly in recent years. The rate of diseased plants in the diseased garden is generally abou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/125
Inventor 王振中孙正祥纪春艳
Owner SOUTH CHINA AGRI UNIV
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