124 results about "Neonicotinoid insecticide" patented technology

Disclosed are mixtures and compositions for controlling invertebrate pests relating to combinations comprising (a) 3-bromo-N-[4-chloro-2-methyl-6-[(methylamino)carbonyl]phenyl]-1-(3-chloro-2-pyridinyl)-1H-pyrazole-5-carboxamide, and its N-oxides, and suitable salts thereof and a component (b) wherein the component (b) is at least one compound or agent selected from neonicotinoids, cholinesterase inhibitors, sodium channel modulators, chitin synthesis inhibitors, ecdysone agonists, lipid biosynthesis inhibitors, macrocyclic lactones, GABA-regulated chloride channel blockers, juvenile hormone mimics, ryanodine receptor ligands, octopamine receptor ligands, mitochondrial electron transport inhibitors, nereistoxin analogs, pyridalyl, flonicamid, pymetrozine, dieldrin, metaflumizone, biological agents, and suitable salts of the foregoing. Also disclosed are methods for controlling an invertebrate pest comprising contacting the invertebrate pest or its environment with a biologically effective amount of a mixture or composition of the invention.

Disclosed are mixtures and compositions for controlling invertebrate pests relating to combinations comprising (a) 3-bromo-N-[4-cyano-2-methyl-6[(methylamino)carbonyl]phenyl]-1-(3-chloro-2-pyridinyl)-1H-pyrazole-5-carboxamide, an N-oxide, or a salt thereof, Formula (1) and (b) at least one invertebrate pest control agent selected from neonicotinoids, cholinesterase inhibitors, sodium channel modulators, chitin synthesis inhibitors, ecdysone agonists, lipid biosynthesis inhibitors, macrocyclic lactones, GABA-regulated chloride channel blockers, juvenile hormone mimics, ryanodine receptor ligands, octopamine receptor ligands, mitochondrial electron transport inhibitors, nereistoxin analogs, pyridalyl, flonicamid, pymetrozine, dieldrin, metaflumizone, biological agents, and salts of the foregoing. Also disclosed are methods for controlling an invertebrate pest comprising contacting the invertebrate pest or its environment with a biologically effective amount of a mixture or composition of the invention.

Disclosed are mixtures and compositions for controlling invertebrate pests relating to combinations comprising (a) 3-bromo-N-[4-cyano-2-methyl-6-[(methylamino)carbonyl]phenyl]-1-(3-chloro-2-pyridinyl)-1H-pyrazole-5-carboxamide, an N-oxide, or a salt thereof, Formula (1) and (b) at least one invertebrate pest control agent selected from neonicotinoids, cholinesterase inhibitors, sodium channel modulators, chitin synthesis inhibitors, ecdysone agonists, lipid biosynthesis inhibitors, macrocyclic lactones, GABA-regulated chloride channel blockers, juvenile hormone mimics, ryanodine receptor ligands, octopamine receptor ligands, mitochondrial electron transport inhibitors, nereistoxin analogs, pyridalyl, flonicamid, pymetrozine, dieldrin, metaflumizone, biological agents, and salts of the foregoing. Also disclosed are methods for controlling an invertebrate pest comprising contacting the invertebrate pest or its environment with a biologically effective amount of a mixture or composition of the invention.

The invention discloses a pesticide composition and application thereof in agriculture. The effective component of the composition comprises flonicamid and neonicotinoid insecticides with a mass ratioof 80:1-1:80. The composition has the advantage of synergetic effect, obviously for resistant pests, reduces the dosage and delays resistance development, and is used for preventing and treating pests with obvious resistance, and is especially suitable for preventing and treating rice planthopper, thrips and aphid with resistance.

The invention relates to a pesticide production and preparation technology, in particular to a sugarcane insecticide. The invention provides a sugarcane insecticide containing a neonicotinoid insecticide, which is prepared into granules or water dispersible granules by mixing the following components in percentage by weight: 0.1-10% of neonicotinoid insecticide, 0.2-15% of organophosphorus insecticide and the balance of assistants, wherein the neonicotinoid insecticide is one of or mixture of any two of thiamethoxam, imidacloprid and thiacloprid; and the organophosphorus insecticide is one or mixture of phoxim and diazinon. When the sugarcane insecticide of the invention is used for preventing and treating sugarcane pests, the sugarcane insecticide has contact poisoning and stomach poisoning functions, has a good systemic conduction function and has a good function of controlling underground and aboveground pests of sugarcane; the sugarcane insecticide which has broad-spectrum insecticidal effects has good effects on preventing and treating sugarcane borers, underground insects, thrips, woolly aphids and the like; the sugarcane insecticide which has a long lasting period can be applied once to effectively eliminate the harm of sugarcane borers in the seedling stage, underground insects, thrips and the like; and the sugarcane insecticide has smaller lethality to natural enemies. The sugarcane insecticide of the invention is used as an important substitute for highly toxic pesticides, has the advantages of low toxicity, high efficiency, safety to people and livestock and is environmental friendly.

The invention relates to a disinsection composition containing a matrine and a neonicotinoid insecticide, and application thereof. The composition mainly uses the matrine and the neonicotinoid insecticide and is prepared by an auxiliary agent and an excipient to form microemulsion, emulsion in water, suspending agent, water dispersible granule, wetting powder and missible oil, wherein the mass percentage of the matrine and the neonicotinoid insecticide is 30:1-1:100. The disinsection composition has higher synergy effect, prevents pests from becoming resistant to drugs, prolongs the service life of pesticides and reduces pesticide residue. The composition can be used for preventing and treating homoptera, lepidoptera, thysanoptera, coleopteran and diptera on crops, particular for rice planthopper, trialeurodes vaporariorum, aphid, tryporyza incertulas, chilo suppressalis, thrips, paddy weevil and gall midge.

The invention relates to a metarhiziumanisopliae compound insecticide composition and use thereof. The metarhiziumanisopliae compound insecticide composition is characterized in that: the active ingredients of the composition include metarhiziumanisopliae and a new neonicotinoid insecticide; the density of the metarhiziumanisopliae in the composition is 1 to 10 billion spores per gram; and the new neonicotinoid insecticide content of the composition is 20 mass percent; and the rest content of the composite are pesticide auxiliary ingredients. The composition has an obvious synergistic effect on grubs and is suitable for preventing and controlling plant pests, particularly grubs. The composition can be used on various crops such as lawns, peanut, sweet potatoes and vegetables or in various places and has the advantages of stable insecticide effect, high quick-acting performance, long efficacy duration, safety and environmental protection.

The invention discloses an insecticidal composition. The insecticidal composition comprises the active ingredients of benfuracarb insecticide and neonicotinoid insecticides comprising any one of thiamethoxam, clothianidin, acetamiprid, imidacloprid and nitenpyram; and the active ingredients can be prepared into wettable powder, granules, water dispersible granules, suspending agents, soluble liquid and suspension seed-dressing seed coating agents. The weight ratio of the active agents comprising the benfuracarb insecticide and neonicotinoid insecticides chosen from any one of thiamethoxam, clothianidin, acetamiprid, imidacloprid and nitenpyram is (1-100):(1-10); the total weight percentage content of the active ingredients is 0.2 to 68 percent; and other ingredients are auxiliary ingredients which are allowable in pesticides. The insecticidal composition has the advantages that: obvious synergism is achieved, using amount of the pesticides is reduced, action range is enlarged, medicine resistance is avoided or medicine resistance is delayed and the like.

The invention provides ochrobactrum intermedium with a collection number CGMCC NO.8839 and a microbial agent. The microbial agent contains bacteria and a culture medium, wherein the bacteria comprise the ochrobactrum intermedium with the collection number CGMCC NO.8839. The invention further provides applications of the ochrobactrum intermedium and the microbial agent to degradation of neonicotinoid insecticides. According to the technical scheme, the residue content of the neonicotinoid insecticides in soil can be effectively reduced.

The invention relates to an insecticide composition containing spinosad and neonicotinoid insecticide and the application thereof. The composition comprises spinosad which is used as a first active ingredient and neonicotinoid insecticide which is used as a second active ingredient; wherein the second active ingredient is selected from imidacloprid or thiamethoxam; the weight ratio between the first active ingredient and the second active ingredient is (0.01-200):1, preferably, (0.05-20) :1. The composition has the advantages that the components are reasonable, the disinsection effect is good, the drug consumption cost is low, the activity and the disinsection effect have remarkable synergistic action but not simple superposition of the activities of all the components, resistance can not be generated, the level of residue of the preparation is low, no pollution can be caused to crops, the security is good, and thereby the composition conforms to the security requirement for pesticide preparations. The composition has 87.11 to 96.04 percent of prevention and control effects on thysanoptera pests, for example, common thrips, and homoptera pests such as lice and aphides.

The invention relates to a pesticide composition of of flubendiamide and neonicotinoid insecticide. The pesticide composition is characterized in that active ingredients comprise a compound A and a compound B, wherein the compound A is flubendiamide, the compound B is at least one of chloronicotinyl insecticides, and the chloronicotinyl insecticides comprise imidacloprid, clothianidin, thiacloprid, dinotefuran, nitenpyram, imidaclothiz and paichongding (IPP). The weight ratio of the compound A to the compound B is 1: 50-50: 1. The insecticide composition can be prepared into common preparation formulations, such as water dispersible granules, wettable powder, suspension concentrate, granules, microcapsule suspension, microcapsule powder, dispersible oil suspension, ultra-low volume liquid, dry flowable and the like according to needs. The insecticide composition has the characteristics of obvious synergy and broad insecticidal spectrum, can effectively control various pests of a variety of crops and can be applied to the various crops, such as vegetables, fruit trees, rice, corn, soybeans, wheat, peanuts, cotton and the like, and has the advantages of high efficiency, quick acting, long acting period and the like.

The invention discloses a method for efficient detection of neonicotinoid insecticides in honey. The method includes steps: (1) standard solution preparation; (2) to-be-detected sample treatment; (3) online solid-phase extraction; (4) high performance liquid chromatography and electrostatic field orbitrap high-resolution mass spectrometric determination. By combination of a high-efficiency high-repeatability online solid-phase extraction method and high performance liquid chromatography and electrostatic field orbitrap high-resolution mass spectrometric determination for detection of the neonicotinoid insecticides in honey, qualitative and quantitative determination of the neonicotinoid insecticides in samples can be realized, and detection results are sensitive and reliable; by adoption of the method, a sample pretreatment process is quick, simple and convenient, and operation efficiency is improved; due to appropriate selection of an extraction solvent, a cleaning agent, an eluent and mass spectrometric detection parameters according to characteristics of the neonicotinoid insecticides, precision and reliability in detection are improved.

The invention relates to an ultra-low volume liquid containing cyantraniliprole and neonicotinoid insecticides. The liquid is compounded by cyantraniliprole and an active ingredient II into a chemical component, and other components are surfactants and solvents; and the active ingredient II is any one of imidacloprid, acetamiprid, nitenpyram, clothianidin, thiamethoxam, thiacloprid or dinotefuran. The ultra-low volume liquid has the advantages of convenience in processing, long duration, high work efficiency, low possibility of generating resistance to pesticides by a target, low environmental contamination, economy of drug and water, and improvement in pesticide utilization rate; and through ultra-low volume spraying, low volume spraying or ultra-low volume static spraying, various pests of rice, wheat, corn, vegetables, tobacco, tea trees, fruit trees and sugarcanes can be prevented and treated.

The invention relates to a control technology specially used for preventing and controlling important forest pest bursaphelenchus xylophilus (monochamus alternatus), namely a microcapsule powder agent or a microcapsule suspending agent prepared by a highly-efficient, harmfulless and less-persistent neonicotinoid insecticide thiacloprid sustained-release preparation and assisted by a high-adhesionadhesive is adopted for prevention and control. The technology adopts an innocuous and degradable carrier as shell materials, is safe to environment, has excellent durable effect and solves the greatproblems of bursaphelenchus xylophilus prevention and control and chemical pesticide pollution.

Disclosed are mixtures and compositions for controlling invertebrate pests relating to combinations comprising (a) 3-bromo-N-[4-chloro-2-methyl-6-[(methylamino)carbonyl]phenyl]-1-(3-chloro-2-pyridinyl)-1H-pyrazole-5-carboxamide, and its N-oxides, and suitable salts thereofanda component (b) wherein the component (b) is at least one compound or agent selected from neonicotinoids, cholinesterase inhibitors, sodium channel modulators, chitin synthesis inhibitors, ecdysone agonists, lipid biosynthesis inhibitors, macrocyclic lactones, GABA-regulated chloride channel blockers, juvenile hormone mimics, ryanodine receptor ligands, octopamine receptor ligands, mitochondrial electron transport inhibitors, nereistoxin analogs, pyridalyl, flonicamid, pymetrozine, dieldrin, metaflumizone, biological agents, and suitable salts of the foregoing.Also disclosed are methods for controlling an invertebrate pest comprising contacting the invertebrate pest or its environment with a biologically effective amount of a mixture or composition of the invention.

The invention discloses an insecticidal composition which comprises an active substance A and an active substance B which are used as effective components, wherein A is tefluthrin, B is one of neonicotine insecticides comprising acetaniprid, imidacloprid and thiamethoxam, and the mass ratio of A to B is 60:1-1:80. The composition of the invention has obvious synergy, can lower the consumption of the effective components and reduce the insecticide applying times, is used for controlling various agricultural pests, and is especially suitable for controlling scarab larvae, flea beetle larvae androot-knot nematode.

The invention discloses a pesticide composition containing spinetoram and neonicotinoid insecticide, which comprises active components composed of a first active component and a second active component, wherein the first active component includes spinetoram and the second active component includes neonicotinoid insecticide selected from imidaclprid, acetamiprid and thiamethoxam, the weight ratio between the first active component and the second active component is (1:20) : (30: 1). The composition can be prepared to be formulations allowed in agriculture such as a water dispersible granule, wettable powder, a suspending agent, an emulsifiable concentrate or a microemulsion. According to the pesticide composition containing spinetoram and neonicotinoid insecticide, the components are reasonable, the insecticidal effect is good, the pesticide cost is low, and the activity and insecticidal effect are not a simple composition of each component activity, compared with the existing single preparation, the pesticide composition containing spinetoram and neonicotinoid insecticide has the advantages of remarkable insecticidal effect, and also prominent synergistic effect, good security, accordance with the safe requirement of pesticide preparations, and good preventing effect for vegetable thrips.

The invention provides an ethylicin-containing pesticidal composition. The pesticidal composition is composed of an effective component (ethylicin) and a neonicotinoid insecticide (imidacloprid, acetamiprid, thiamethoxam, thiacloprid, nitenpyram, dinotefuran or clothianidin), and in combination with auxiliary components such as additives and carriers and the like which are usually used in pesticides, the pesticidal composition can be processed into micro-emulsions, wettable powder and water dispersible granules. By using the pesticidal composition provided by the invention, a higher synergistic effect can be generated, the production of drug resistance of pests can be reduced and delayed, and the service life of a pesticide variety can be prolonged. The composition provided by the invention can be used for preventing and controlling various homoptera and thysanoptera injurious insects and the like on rice, vegetables and fruit trees and the like, especially can be used for preventing and controlling rice planthopper, aphids, whiteflies, scale insects, thrips, rice gall midges, and the like; and meanwhile, the pesticidal composition also can be used for treating crop diseases such as rice blasts, cotton fusarium wilts, and the like.

A method of treatment and a package that is designed to accomplish this. The package provides the pet owner with a pack containing individual doses of topically applied medications pre-packaged with an oral taste treat. The topically applied medication contains at least one pharmaceutically active ingredient selected from the group of chemical families comprising phenylpyrazoles, macrocyclic lactones, neonicotinoids, synthetic pyrethroids, insect growth regulators, amidines, semicarbazones, spinosyns, octadepsipeptides, and pyrazinoisoquinolines, or any combination thereof. The treat can be administered either before the topically applied treatment is given so as to relax and distract the pet, or after the treatment is given so as to reward the pet and help it forget the stress of treatment. The treat can also be shaped to signify the type of animal to be treated.

The invention discloses an insecticide composition with chlorfenapyr and neonicotinoid insecticides, and a preparation and application of the insecticide composition. The insecticide composition comprises the chlorfenapyr and the neonicotinoid insecticides. The neonicotinoid insecticides are an optional type of imidacloprid, thiamethoxam, clothianidin, nitenpyram, thiacloprid, dinotefuran and acetamiprid. A mass ratio of the chlorfenapyr to the neonicotinoid insecticides is (0.05-20):1. An insecticide comprises the insecticide composition and auxiliary materials acceptable to the insecticide preparation with the insecticide composition. A mass percent content of the insecticide composition in the insecticide is 2%-6%. The insecticide composition, the preparation and the application have the advantages that obvious synergism effects can be realized for bradysia odoriphaga; the chlorfenapyr and the neonicotinoid insecticides have different action mechanisms, accordingly, the insecticide composition is beneficial to delaying development of pest resistance to the insecticide, and the service lives of the chlorfenapyr and the neonicotinoid insecticides can be prolonged; dosage of the neonicotinoid insecticides can be reduced, and accordingly neonicotinoid insecticide residual risks can be reduced; the preparation in various dosage forms is made of the insecticide composition, and accordingly excellent prevention and control effects can be realized for the bradysia odoriphaga.

The invention provides an environment-friendly insecticide, which is prepared from the following components by mass percent: 0.1-40% of thiamethoxam, 50-78% of holly essential oil, 5-30% of neem oil, 1-10% of an emulsifying agent and 0.1-0.8% of an antioxidant. The insecticide prepared by effectively combining neonicotinoid insecticides, the neem oil and the holly essential oil can be used for controlling pests, and has a killing effect specially for the common pests such as aphids, thrips, scale insects and tetranychus mites on Chinese orchid; head oil and the tail oil of the plant essential oil are used for replacing a toxic organic solvent used in the preparation process of emulsifiable concentrate, and so that the cost of the pesticide is lowered. The plant essential oil is rapidly and thoroughly degraded in the environment, is free from residue, has no pollution to the environment, and can improve the pesticide activity and reduce pesticide consumption; furthermore, the plant essential oil is mixed with the neem oil, so that the absorption and conduction effects of the Chinese orchid for the insecticide are better promoted, and a synergistic effect for the thiamethoxam is achieved.

The invention relates to an antibiotic seed treatment agent composition. The composition comprises active ingredients, auxiliaries and carriers, wherein the active ingredients include effective quantity of neonicotinoid insecticide I ingredient and antibiotic bactericide II ingredient; the weight percent of the active ingredients in the composition is 1-20%; the I ingredient accounts for 1-10% of the composition by weight; the II ingredient accounts for 1-10% of the composition by weight. The antibiotic seed treatment agent composition has the beneficial effects that the two active ingredients are used after compounding, thus improving the crop disease control effects; insecticide / bactericide seed treatment agents are prepared from the seed treatment agent composition and the preferentially selected carriers and auxiliaries, so that the film forming property and the water resistance of films are greatly improved and the active ingredients are released slowly, thus lengthening the lasting periods of the active ingredient drugs, increasing the germination rates of seeds and promoting healthy growth of seedlings, reducing the consumption of pesticides and reducing pesticide residues and environmental pollution.

The invention relates to a composition of an agricultural synergist and neonicotinoid insecticides. The composition comprises the agricultural synergist and one or multiple of the neonicotinoid insecticides, wherein NF-100 is adopted as the agricultural synergist, one or multiple of imidacloprid, acetamiprid, nitenpyram, thiacloprid, thiamethoxam and dinotefuran is / are selected as the neonicotinoid insecticides, and the weight ratio of the agricultural synergist to the neonicotinoid insecticides is 1:5-200.

The invention relates to the technical field of pesticides, and in particular relates to an insecticide composition containing thiobenzamide and a neonicotinoid insecticide. The insecticide composition is composed of thiobenzamide and the neonicotinoid insecticide, wherein thiobenzamide is 3-bromo-N-[4-chloro-2-methyl-6-(methylaminothioformyl) benzene]-1-(3-chloropyridine-2-yl)-1H-pyrazol-5-formamide; the neonicotinoid insecticide is any one of dinotefuran, clothianidin, thiamethoxam, imidacloprid, cycloxaprid, acetamiprid, nitenpyram, imidaclothiz or thiacloprid; and the mass ratio of thiobenzamide to the neonicotinoid insecticide ranges from (1:50) to (50:1). The insecticide composition and the insecticide thereof have the obvious synergistic effect when compared with a single dosage, and can be used for effectively preventing and treating various crop insects; and the use dosage of pesticides is reduced, and the drug use cost is saved.

The invention discloses plant-derived pesticide synergist. The synergist comprises leguminous plant astragatus complanatus extracts. The astragatus complanatus extracts are prepared from a solvent extraction method or an supercritical fluid extraction method. According to application of the plant-derived pesticide synergist in chemical pesticide synergism, the good synergism is achieved on multiple types of chemical insecticides such as pyrethroid insecticides, organic phosphorous insecticides, carbamate insecticides, neonicotinoid insecticides, pyrrole insecticides, pyrazole insecticides andpyridine insecticides, and the dosage of the chemical pesticide can be lowered in multiples while the same control effect is achieved. In addition, the plant-derived pesticide synergist is safe to people and livestock and environmentally friendly and facilitates comprehensive utilization of astragatus complanatus.

The invention relates to a pesticide-fertilizer composition. The pesticide-fertilizer composition includes the following components in percentage by weight: 70 to 95 percent of fertilizer components, 1 to 20 percent of pesticide components, and 1 to 10 percent of additives, wherein fertilizers include one or more kinds of the followings: organic fertilizers, inorganic fertilizers, compound fertilizers, organic-inorganic compound fertilizers, and microelement fertilizers; pesticides include neonicotinoid insecticides and / or organophosphorus insecticides; the additives include one or more kinds of the followings: solvents, surfactants, and binders.

The invention relates to baits for controlling flies (fly gels) comprising a) one or more neonicotinoids, b) one or more gelling agents, c) one or more attractants, and having a gel point between having a Brookfield yield value between 150 and 1000 and a Brookfield viscosity between 40,000 and 150,000 mPa·s, to a process for the preparation of such fly gels, its use and methods for controlling flies.

The invention relates to a synergistic pesticide composition containing chlorfenapyr fluorobenzamide bisamide and neonicotinoid insecticide. The active ingredient is a binary compound of the chlorfenapyr fluorobenzene bisamide and the neonicotinoid insecticide. The mass ratio of the chlorfenapyr fluorobenzene bisamide to the neonicotinoid insecticide is 1:50-50:1, the mass ratio of the active ingredient chlorfenapyr fluorobenzene bisamide to the flunicarbamide is 1%-80%, and the rest is an auxiliary component; the dosage form of an insecticidal composition is a water-dispersible granule, wettable powder, a suspending agent, an emulsifiable concentrate, a microemulsion or an an aqueous emulsion, and the composition is mainly used for controlling hemiptera, lepidoptera and coleoptera such ashibiscus, planthopper, whiteflies, spider mites, mites, blind mites, scale insects, mites, cotton bollworm, diamondback moth, grass worms, leaf roller moths, heartworm, potato beetles and the like. The composition expands the insecticidal spectrum of a single agent, and has a significant synergistic effect, and the insecticidal activity is obviously enhanced than the single agent, which reduces the dosage of a pesticide and reduces the environmental pollution.

The invention belongs to the technical field of insecticide detection, and discloses a water neonicotinoid insecticide and a solid phase extraction detection method for the transformation product thereof. The method particularly comprises the following steps: (1) a solid phase extraction cartridge is activated; (2) a recovery indicator is added after the pH value of a water sample is adjusted, andin the solid phase extraction cartridge, the water sample is extracted; (3) after vacuum drying on the solid phase extraction cartridge, elution is performed to obtain an eluent, and an internal standard compound is added; and (4) the concentration of a target compound is detected. The self-loading mixed adsorbent solid phase extraction cartridge operation method adopted in the invention is simple and convenient, the water neonicotinoid insecticide and the transformation product thereof can be analyzed at the same time, good adsorption performance is achieved for the neonicotinoid insecticideand the transformation product thereof, the detection limit in surface water is 0.56 to 5.43ng / L, and the matrix effect is -7.58% to 21.1%.

The invention discloses a method for detecting neonicotinoid insecticides and metabolites in urine by solid phase extraction-ultra-high performance liquid chromatography-tandem mass spectrometry. The method comprises the following steps: (1) preparing an internal standard stock solution and a target analyte standard stock solution; (2) pretreating a urine sample to be detected: 1) performing enzymolysis; 2) performing solid-phase extraction, separation and purification: a) activating a solid-phase extraction small column; b) loading a sample; c) performing elution: eluting the substance to be detected by using a dichloromethane-acetonitrile mixture, wherein the volume ratio of dichloromethane to acetonitrile is (8-5):(2-5); 3) extracting, crystallizing and removing impurities: blow-drying eluent, adding the dichloromethane-acetonitrile mixture to dissolve residues, centrifuging, taking supernate, blow-drying, and re-dissolving with methanol-formic acid water to obtain re-dissolved liquid, and obtaining the methanol-formic acid water by mixing methanol and a formic acid water solution with the concentration of 0.15% according to the volume ratio of (1-2):(9-8); 4) filtering with a filter membrane; (3) analyzing a urine sample; (4) drawing a standard curve; and (5) performing data analysis.