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Ochrobactrum intermedium, microbial agent and applications of ochrobactrum intermedium and microbial agent

A technology of Bacillus intermedius and microbial inoculum, applied in the field of agricultural biology, can solve problems such as poor effect, and achieve the effect of efficient degradation and reduction of residues

Active Publication Date: 2014-07-23
YANTAI DIYUAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The application of microbial degradation to degrade nicotinic pesticides has the advantage of being environmentally friendly, but at present, the effect of existing microbial strains on the degradation of nicotinic pesticides is still poor

Method used

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  • Ochrobactrum intermedium, microbial agent and applications of ochrobactrum intermedium and microbial agent
  • Ochrobactrum intermedium, microbial agent and applications of ochrobactrum intermedium and microbial agent

Examples

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preparation example Construction

[0020] Wherein, the preparation method of the microbial bacterial agent may include: inoculating the Bacillus intermedium of CGMCC No. 8839 in the fermentation medium for culturing. Preferably, in each gram of the microbial agent obtained by culturing, the number of viable bacteria of Paleobacterium intermedia whose preservation number is CGMCC NO.8839 can be (5-200)×10 7 CFU, more preferably (20-120)×10 7 CFU. Wherein, the cultivation temperature may be 28-35° C., and the cultivation time may be 10-50 hours.

[0021] Preferably, the preparation method of the microbial agent may also include: mixing the material obtained after culturing in the fermentation medium with an inert solid carrier and a binder; the inert solid carrier includes clay, silicon dioxide, silicon At least one of algal earth, kaolin, attapulgite, montmorillonite, bentonite, talc, white carbon black and calcium carbonate; the binder includes maltose and / or dextrin. Preferably, the amount of the inert soli...

Embodiment 1

[0026] This example is used to illustrate the cultivation of Pallidum intermedia and the preparation of microbial inoculum of the present invention.

[0027] Inoculate Ochrobactrum intermedium (Ochrobactrum intermedium) with preservation number CGMCC NO.8839 into LB medium (containing tryptone of 10g / L, yeast extract of 5g / L and NaCl of 10g / L), at 30°C and Cultivate to bacterial density OD under shaking at 200 rpm 600 The value is 0.6-0.8, and the seed bacterial liquid is obtained.

[0028] 100mL of above-mentioned seed bacterium liquid is added in 100L fermentation medium (fermentation medium contains the corn flour of 80g / L, the starch of 80g / L, the sucrose of 80g / L, the soybean oil of 40g / L, the sodium chloride of 3g / L, 3g / L of calcium carbonate, 5g / L of potassium dihydrogen phosphate and 5g / L of ferrous sulfate), cultured at 30°C, samples were taken during the culture and observed by direct counting under a microscope until each gram of culture The viable count of Paleob...

Embodiment 2

[0030] This example is used to illustrate the cultivation of Pallidum intermedia and the preparation of microbial inoculum of the present invention.

[0031] Inoculate Ochrobactrum intermedium (Ochrobactrum intermedium) with preservation number CGMCC NO.8839 into LB medium (containing tryptone of 10g / L, yeast extract of 5g / L and NaCl of 10g / L), at 30°C and Cultivate to bacterial density OD under shaking at 200 rpm 600 The value is 0.6-0.8, and the seed bacterial liquid is obtained.

[0032] 100mL of above-mentioned seed bacterium liquid is added in 100L fermentation medium (fermentation medium contains the corn flour of 80g / L, the starch of 80g / L, the sucrose of 80g / L, the soybean oil of 40g / L, the sodium chloride of 3g / L, 3g / L of calcium carbonate, 5g / L of potassium dihydrogen phosphate and 5g / L of ferrous sulfate), cultured at 30°C, samples were taken during the culture and observed by direct counting under a microscope until each gram of culture The viable count of Paleob...

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Abstract

The invention provides ochrobactrum intermedium with a collection number CGMCC NO.8839 and a microbial agent. The microbial agent contains bacteria and a culture medium, wherein the bacteria comprise the ochrobactrum intermedium with the collection number CGMCC NO.8839. The invention further provides applications of the ochrobactrum intermedium and the microbial agent to degradation of neonicotinoid insecticides. According to the technical scheme, the residue content of the neonicotinoid insecticides in soil can be effectively reduced.

Description

technical field [0001] The invention relates to the field of agricultural biotechnology, in particular to a Pseudomonas intermedia, a microbial agent and applications thereof. Background technique [0002] Nicotinic insecticides are acetylcholinease receptor inhibitors that act on the central nervous system of insects. They are highly efficient, selective, and safe for mammals, and there is no cross-resistance with most traditional pesticides. Among them, the neonicotinoid compound with insecticidal activity was first discovered by Shell Company, and then the structure was continuously optimized to obtain clothianidol with high insecticidal activity. Research. Currently commercialized neonicotinoid insecticides include imidacloprid, acetamiprid, nitenpyram, chlorothiaprid, thiacloprid, thiamethoxam, clothianidin and dinotefuran. [0003] The output of nicotine pesticides accounts for 15-20% of the global pesticide output. The extensive use of nicotine pesticides has cause...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C02F3/34C12R1/01C02F101/34C02F101/38C02F101/36
Inventor 赵君才阮志勇赵秉强王慧敏赵文勇吕昭辉刘慧宋昭王彦伟刘小飞
Owner YANTAI DIYUAN BIOTECH
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