42 results about "CAR bacillus" patented technology

The present invention relates to the isolation of polypeptides derived from the Clostridium botulinum neurotoxin and the use thereof as immunogens for the production of vaccines and antitoxins, as well as research and drug development applications. Clostridium botulinum is responsible for food bone botulism, a severe and often deadly disease. Botulinum neurotoxin binds to neural cells and are translocated into the cytosol. The toxin then prevents neurotransmitter release by cleaving a SNARE protein. A double mutant E224A / E262 full length botulinum neurotoxin Type A holo form was successfully cloned and purified, which lacks the endopeptidase activity involved in the toxic action of the BoNT, and thus leading to its detoxification (DR BoNT / A). This new molecule can be used as an antidote against botulism, and also as a vaccine candidate for botulism. Due to the poor availability and extreme toxicity of native holo-toxin, a nontoxic form of the holo-toxin is highly desired for research and vaccine development. The full length DR BoNT / A protein has been expressed in E. coli as a soluble form.

The present invention discloses a Proteus mirabilis strain and a method for producing S-equol through daidzein conversion by using the Proteus mirabilis strain, wherein the strain is preserved in the China Center for Type Culture Collection on November 13, 2011, and a preservation number is CCTCC M 2011390. In the prior art, the existing technology is difficult to prepare the S-equol through conversion. With the present invention, the problem in the prior art is solved, and a single function microorganism strain for directly converting a prochiral compound daidzein into a chiral compound S-equol and a preparation method thereof are provided; and compared with the strictly anaerobic equol conversion strains and the mixing bacteria conversion in the previous reports, the Proteus mirabilis LH-52 strain of the present invention is a facultative anaerobe, and is more suitable for industrial production.

The invention discloses an EPSP synthase gene derived from ochrobactrum anthropi and application thereof. The EPSP synthase gene contains 1353 basic groups and 451 coded amino acids, the nucleotide sequence of the EPSP synthase gene is as shown in SEQ ID NO1, and the amino acid sequence of the coded proteins is as shown in SEQ ID NO2. The EPSP synthase gene derived from ochrobactrum anthropi disclosed by the invention is synthesized by a manual method, has high homology with the reported EPSP synthase gene derived from agrobacterium tumfaciens, has higher glyphosate tolerance, and can be used for culturing genetically modified crops.

The invention belongs to the technical field of biological fermentation and discloses a multi-stage rotating speed regulating policy for improving pseudomonas denitrificans fermentation for productionof vitamin B12. A pseudomonas denitrificans strain is fermented and cultured in a fermenting culture medium. One litter of the culture medium contains 70-85g of saccharose, 40-55g of corn steep liquor, 10-20g of glycine betaine, 0.02-0.05g of auxin, 0.01-0.05g of tryptophan and 0.05-0.1g of glutamine and the like; if the fermentation time is 0-25h, the stirring rotating speed is 150-280 rpm; if the fermentation time is 26-90 h, the stirring rotating speed is 300-400 rpm; if the fermentation time is 91-185h, the stirring rotating speed is 100-200 rpm; vitamin B12 is separated and purified fromfermentation liquor. By controlling the stirring rotating speed and the components of the fermenting culture media, the output of vitamin B12 is improved.

The invention discloses a new bacteria strain-bacteroides uniformis L8 and an application thereof in degrading agar or agar oligosaccharide. The bacteria strain can degrade agar with molecular weight of 100-300kDa into agar oligosaccharide and further degrade the agar oligosaccharide into D-galactose, or degrade the agar oligosaccharide with molecular weight of 0.5-10kDa into D-galactose finally, in addition, the agar oligosaccharide has the specificity to promote growth of the bacteroides uniformis, so as to play a role in regulating associated symptoms caused by obesity.

The invention discloses an automatic mycobacterium tuberculosis screening system for mycobacterium tuberculosis sputum smear, comprising a mycobacterium tuberculosis sample scanner, a control recognition system and a remote diagnosis system, wherein the mycobacterium tuberculosis sample scanner comprises an optical system for amplifying a mycobacterium tuberculosis sample, a high-precision slide glass platform for holding the mycobacterium tuberculosis sample, an illumination system and a control PC machine; the control recognition system comprises a mycobacterium tuberculosis sample scanning system, an intelligent mycobacterium tuberculosis recognition system and a mycobacterium tuberculosis image database management system; the control PC machine is in communication connection with the mycobacterium tuberculosis sample scanning system; the mycobacterium tuberculosis image database management system is connected with the intelligent mycobacterium tuberculosis sample recognition system by a mycobacterium tuberculosis sample image display and analyzing application module; the control recognition system is connected with the remote diagnosis system. According to the invention, the mycobacterium tuberculosis sputum smear is successfully digitalized by a fluorescent scanner, automatic scanning is realized, remote share of one mycobacterium tuberculosis sputum smear can be realized, and the mycobacterium tuberculosis sputum smear can be stored for a long time.

The invention discloses a construction method and application of a tubercle bacillus Pup gene deletion mutation strain. Based on a fusion PCR technique and a T carrier cloning technique, a replacementtype deletion mutation carrier T-Pup-N-K-Pup-C of a gene is constructed and identified for the first time, through an electrotransformation technique, the recombinant Pup gene deletion mutation carrier enters mycobacterium tuberculosis competent cells, and through chromosome homologous recombination, multiple screening and identification, the tubercle bacillus Pup gene deletion mutation strain containing a kanamycin resistant gene is obtained for the first time. According to the tubercle bacillus Pup gene deletion mutation strain constructed by the construction method disclosed by the invention, a Pup gene in an MTB Pup-proteasome system is knocked out, so that the drug tolerance of a clinical drug-tolerance tubercle bacillus separation strain can be reduced, the clinical drug-tolerance tubercle bacillus separation strain can restore sensibility to tubercle resisting medicines, the ubercle bacillus Pup gene deletion mutation strain can help for exploring the pathogenic mechanism of MTB and controlling propagation of tuberculosis, and a new direction is provided for seeking a tubercle resisting drug target.

The present invention discloses a polypeptide with tubercle bacillus immunogenicity, which contains a sequence shown by Seq ID No.2 in the sequence table. The present invention also discloses a kit containing the polypetide as above. When the polypeptide and the kit are adopted to detect the infectivity of tubercle bacillus, the false positive rate is greatly decreased, while the true positive rate can be kept unchanged.

The invention belongs to the technical field of biology and particularly discloses a method for efficiently degrading azoxystrobin by utilizing ochrobactrum anthropic. Ochrobactrum anthropic SH14 is used for degrading the azoxystrobin under the condition that the temperature is 30.2 DEG C, the pH is 7.9 and the inoculum size is 0.2g / L, and the degradation rate of the ochrobactrum anthropic SH14 on 50mg / L of azoxystrobin is above 85%; the method disclosed by the invention provides theoretical practice basis for future large-scale industrial production and application of the ochrobactrum anthropic SH14 and has wide signification.

The invention relates to a composite probiotics feed additive containing lactic acid bacteria. The composite probiotics feed additive is prepared by spraying, freezing, drying and compounding 40-60% of lactobacillus acidophilus, 15-20% of lactobacillus amylophilus, 5-10% of lactobacillus salivarius, 5-10% of streptococcus thermophilus, 5-10% of bacteroides thetaiotaomicron and 5-10% of glucose. All strains are separated from pig intestines chime. With the consideration of each part of an intestinal canal of animal, the lactic acid bacteria which produce lactic acid of the front section of the intestinal canal are combined with the bacteroides thetaiotaomicron which dissolve cellulose and polysaccharide of the rear section of the intestinal canal, the proportion among the bacteria is similar to the composition of corresponding bacteria in the intestinal canal of a healthy animal, so that the respective advantages can be developed well, the health of the animal is improved further, the feed digestibility is improved, and the production performance of the animal is improved.

The present invention is one kind of natural cell growth regulating factor F and its preparation process. The preparation process includes screening Gram-positive coccus or bacillus and Gram-negative coccus or bacillus, freeze preserving coccus or bacillus strain, conventional culture of the coccus or bacillus strain at 4-38 deg.c, once filtering to eliminate harmful bacteria while leaving beneficial thallus segment, extracting natural gene, adding alanine into the natural gene, safety test, preparing into solution or powder, and sealing. The cell growth regulating factor F has the functions of stimulating and strengthening immunity, strengthening TC, repairing and reforming necrotic, degenerative, atrophic, fibrillated and calcified pancreatic histiocyte, regenerating capillary vessel, so that it can repair and reform skin and soft tissue.

The invention discloses wheat ochrobactrum of degradable lincomycin. The wheat ochrobactrum is preserved in China General Microbiological Culture Collection Center as the preservation number CGMCC No.10665. The wheat ochrobactrum of the degradable lincomycin can use the lincomycin as an exclusive carbon source to grow, and the degradation capacity of the strain for the lincomycin can reach 100 percent in 24 hours. The strain can be used for harmlessly treating fermented fungi residue in the industrial production of the lincomycin, the treatment method is effective, environment-friendly, capable of saving the resource, low in treatment cost and capable of thoroughly eliminating adverse impact of lincomycin waste hypha on the environment.

The invention relates to a double-antibody enzyme-linked immunosorbent assay for detecting clove pseudomonas spot pathogenic and variant bacteria, which belongs to the field of the immunoassay. Inactivated clove pseudomonas spot pathogenic and variant inactivated bacteria are used for immunizing an 8-month-old BALB / c mouse, and six hybridoma cell strains for producing specific monoclonal antibodies can be obtained by virtue of immunization, cell fusion and screening. The six antibodies are respectively marked by HRP, and every two of the six antibodies are paired by adopting the clove pseudomonas spot pathogenic and variant bacteria as a target. The antibody 1D3 (CGMCCNo.9312 monoclonal cell strain Q) and the antibody 6C6 (CGMCCNo.9313 monoclonal cell strain R) are respectively used as a coating antibody and an ELISA antibody to establish the double-antibody enzyme-linked ELISA method of the clove pseudomonas spot pathogenic and variant bacteria, and LOD is 1.5*10<5>cfu / mL. By adopting the ELISA method to detect the double-antibody enzyme-linked immunosorbent assay for detecting clove pseudomonas spot pathogenic and variant bacteria, the specificity is good, the sensitivity is high, the cost is low, and the high-flux determination of the double-antibody enzyme-linked immunosorbent assay for detecting clove pseudomonas spot pathogenic and variant bacteria can be realized.

The invention discloses an EPSP synthase gene derived from ochrobactrum anthropi and application thereof. The EPSP synthase gene contains 1353 basic groups and 451 coded amino acids, the nucleotide sequence of the EPSP synthase gene is as shown in SEQ ID NO1, and the amino acid sequence of the coded proteins is as shown in SEQ ID NO2. The EPSP synthase gene derived from ochrobactrum anthropi disclosed by the invention is synthesized by a manual method, has high homology with the reported EPSP synthase gene derived from agrobacterium tumfaciens, has higher glyphosate tolerance, and can be usedfor culturing genetically modified crops.