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Haemophilus parasuis disease and swine pasteurella multocida disease combined vaccine and preparation method thereof

A technology for Haemophilus suis disease and Haemophilus suis, applied in antibacterial drugs, pharmaceutical formulations, bacterial antigen components, etc., can solve problems such as different immune procedures, and achieve the effect of long immune protection period and good immune effect.

Active Publication Date: 2014-02-12
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention solves the problem of different immunization procedures of Haemophilus parasuis single vaccine and Pasteurella multocida single vaccine, and finds that the two have a mutual promotion effect on immune effect. The vaccine of the invention has high safety performance, high immunogen Good sex, long immunization period, etc.; and can reduce costs, reduce immunization labor, reduce animal stress, and can meet the requirements of different users

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: the preparation of double vaccine

[0030] 1. Preparation of seeds for production

[0031] Propagation of primary seeds: Haemophilus parasuis serotype 4 JS strain and type 5 ZJ strain freeze-dried strains were streaked and inoculated on TSA / NAD (TSA was produced by BD Company, NAD was produced by Roche Company) plates, Cultivate at 37°C for 18-24 hours, select colonies that meet the requirements, inoculate them in TSB / NAD (TSB is produced by BD Company, NAD is produced by Roche Company) liquid medium, and cultivate at 37°C for 12-16 hours, as the primary seed ; Pasteurella multocida type A HN5 strain, Pasteurella multocida type B type C44-1 strain and Pasteurella multocida type D type HB4 strain freeze-dried strains were streaked and inoculated in TSA (10% chicken serum ) (chicken serum was produced by Zhengzhou Bai'an Bioengineering Co., Ltd.) plates, and cultured at 37°C for 18-24 hours as first-class seeds.

[0032] Propagation of secondary seeds: Tak...

Embodiment 2

[0044] Embodiment 2: Sterility test and safety test of dual vaccine

[0045] 1. Sterility test

[0046] According to the method on page 50 of the appendix of "Regulations of Veterinary Biological Products of the People's Republic of China" 2000 edition, the vaccine prepared by the present invention was sterility tested; the result was that no colony growth was seen in the vaccine obtained.

[0047] 2. Safety test

[0048] Safety test on mice: Select 10 3-week-old BALB / c mice and divide them into two groups, 5 mice in each group. One group is injected with 0.5ml of this inactivated vaccine subcutaneously, and the other group is injected with 0.1ml in the thigh muscle. Observation after 10 days. As a result, the mice were in a good state of mind, with no adverse reactions and no deaths.

[0049] Piglet safety test: select 10 healthy susceptible piglets within 30 days of age, randomly select 5 piglets for neck intramuscular injection with the double dose according to usage (th...

Embodiment 3

[0051] Embodiment 3: Efficacy test of dual vaccine

[0052] 50 healthy and susceptible 3-week-old weaned piglets were selected and divided into two groups, 25 in the immunization group and 25 in the control group. 3-week-old piglets were immunized for the first time, with an interval of 2 weeks, and booster immunization was carried out at the age of 5 weeks, with an interval of 2 weeks, and each virulent strain was used to challenge the virus at the age of 7 weeks; the vaccine dosage for each immunization was 2ml. The finished product of the combined vaccine; after the challenge, observe the statistical results for 14 days.

[0053] Haemophilus parasuis JS strain: Select 5 immunized pigs, together with 5 unimmunized pigs with the same conditions, intraperitoneally inject Haemophilus parasuis JS strain, the challenge dose is 5.0×10 9 CFU / head, observed for 14 days after challenge, more than 4 / 5 of the immune piglets were protected, and more than 4 / 5 of the control piglets were...

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Abstract

The invention provides a haemophilus parasuis disease and swine pasteurella multocida disease combined vaccine which comprises at least one haemophilus parasuis antigen and at least one swine pasteurella multocida antigen, preferably, the haemophilus parasuis antigen at least comprises type 4 serotype and type 5 serotype, and the swine pasteurella multocida antigen at least comprises type A capsule serotype, type B capsule serotype and type D capsule serotype. By using the combined vaccine provided by the invention, the haemophilus parasuis antigen and the swine pasteurella multocida antigen do not generate mutual interference or influence, a vaccine composition containing the haemophilus parasuis antigen and the swine pasteurella multocida antigen not only can generate protective immune response to two kinds of bacilli, but also makes the haemophilus parasuis antigen and the swine pasteurella multocida antigen generate mutually-enhanced immune effect. The combined vaccine provided by the invention has an immunization protection stage as long as one year. The invention also correspondingly provides a preparation method of the combined vaccine.

Description

technical field [0001] The invention relates to a dual vaccine against Haemophilus parasuis and Pasteurella multocida and a preparation method thereof. Background technique [0002] Haemophilus parasuis and pasteurellosis are two major bacterial infectious diseases in pig herds in my country. [0003] Haemophilus parasuis (Hps) disease, also known as porcine Glasser disease, affects young pigs aged 2 weeks to 4 months, mainly around weaning and nursery stages, and is usually seen in pigs aged 5 to 8 weeks. The incidence rate is generally 10-15%, and the mortality rate can reach 50% in severe cases. The main clinical symptoms were cough, dyspnea, emaciation, lameness, and rough coat; the main autopsy lesions were pleurisy, pericarditis, peritonitis, arthritis, and meningitis. In addition, Haemophilus parasuis can also cause sepsis, cyanosis, subcutaneous edema and pulmonary edema, and even death in the absence of typical serositis. Haemophilus parasuis acute infection may ...

Claims

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Application Information

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IPC IPC(8): A61K39/116A61P31/04A61K39/102
Inventor 张许科孙进忠白朝勇
Owner PU LIKE BIO ENG
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