49 results about "Pasteuria" patented technology

The subject invention provides novel and advantageous methods for growing bacteria. The methods of the subject invention are particularly advantageous for growing parasitic bacteria in vitro, without the presence of host tissue. In one embodiment of the subject invention, Pasteuria endospores, such as those that infect the rootknot nematode Meloidogyne arenaria or other host nematodes, are grown in vitro under acidic conditions. The process of the subject invention is highly advantageous because Pasteuria can be grown in the absence of nematode tissue.

The subject invention provides novel and advantageous materials and methods for controlling phytopathogenic and / or soil-dwelling nematodes by attaching an effective amount of Pasteuria spores to a seed and delivering the seeds to the situs of nematodes.

The invention discloses a preparation method of a nucleic acid vaccine for fowl cholera, which comprises the following steps of: designing primers by a fowl cholera bacterium genome sequence; carrying out PCR (polymerase chain reaction) amplification by taking a fowl cholera bacterium CVCC474 strain genome as a template to obtain a target gene; carrying out enzyme digestion on the target gene and an eukaryotic expression vector pcDNA3.1(+) by utilizing a restriction enzyme KpnI and a restriction enzyme EcoRI; then connecting the target gene with the eukaryotic expression vector pcDNA3.1(+); converting into the Escherichia coli competence JM83; extracting plasmids; and carrying out enzyme digestion identification to obtain the nucleic acid vaccine for fowl cholera. The animal experiment detection shows that the nucleic acid vaccine for fowl cholera prepared by the method disclosed by the invention can reduce the occurrence of fowl cholera and can reduce the intrusion of fowl cholera bacteria on fowls; and the preparation method of the nucleic acid vaccine is simple and easy to operate.

The invention discloses a preparation method for an avian pasteurella genome vaccine. The preparation method comprises the following steps of: performing enzyme digestion on a target gene by using a restrictive incision enzyme Sau3AI; performing enzyme digestion on an eucaryon expression vector PcDNA 3.1(+) by using a restrictive incision enzyme BamHI; performing dephosphorylation on the eucaryon expression vector PcDNA 3.1(+) which is subjected to enzyme digestion by using calf intestinal alkaline phosphatase; connecting an avian pasteurella genome with the eucaryon expression vector pcDNA3.1(+); transform into a colon bacillus competence JM83; extracting plasmids; and identifying through enzyme digestion to obtain the avian pasteurella genome vaccine. As proved by animal experiment detection, the avian pasteurella genome vaccine prepared with the method can be used for reducing the generation of avian pasteurellosis and reducing the invasion of avian pasteurella to birds; and the preparation method is simple and is easy to operate.

The invention provides a propolis-adjuvant inactivated vaccine for mink pasteurellosis and a method for preparing the propolis-adjuvant inactivated vaccine. The method includes: vaccinating mink original pasteurella onto a blood agar slant, culturing for 24 hours at 37 DEG C, checking purity, using as seed pasteurella liquid vaccinated blood agar matrix, inactivating to obtain an aqueous phase to be fully mixed with propolis adjuvant to obtain the propolis-adjuvant inactivated vaccine for mink pasteurellosis. The propolis-adjuvant inactivated vaccine can effectively prevent mink pasteurellosis, and adverse reactions after vaccination and influences on fur quality can be evidently reduced to create conditions for control of fur-bearing animal diseases.

The invention relates to a culture method for increasing the yield of the capsule of avian pasteurella multocida. The culture method comprises the following steps: (1) inoculation: inoculating avian pasteurella multocida into a triangular flask with a culture medium; (2) shake culture: putting the triangular flask after inoculation in the step (1) into an incubator to undergo shake culture at a temperature of 36-38 DEG C for 5.5-6.5 hours, wherein the shaker speed is 180-220rpm; (3) stationary culture: putting the triangular flask after shake culture in the step (2) into the incubator to stand, wherein the culture temperature is 36-38 DEG C and the stationary culture time is 7.5-8.5 hours. The yield of the capsule of avian pasteurella multocida can be obviously increased by adopting the culture method provided by the invention.

The invention discloses an anti-Mycoplasma bovis and Pasteurella fusion protein Md-UF1-Md-AP2 and a gene encoding the fusion protein. It induces housefly larvae with Mycoplasma pneumoniae and constructs an inhibitory subtractive library. In the inhibitory subtractive library The Md-UF1 gene screened out in the chicken-derived Salmonella housefly larvae was used to construct a suppressive subtractive library, and the Md-AP2 gene screened out in the suppressive subtractive library was constructed using the overlap extension and shearing technique. The fusion gene Md-UF1-Md-AP2, the expressed anti-Mycoplasma bovis and Pasteurella fusion protein Md-UF1-Md-AP2, has inhibitory effects on Pasteurella multocida type A and Mycoplasma bovis, The problem of drug resistance of Pasteurella bovis and Mycoplasma bovis has been solved. When the cattle have pneumonia symptoms, early medication can prevent and treat at the same time.

The present invention discloses one kind of live vaccine of fowl Pasteur's multocidum bacterium and its preparation process, and belongs to the field of biological product technology. The live vaccine is prepared with natural low virulent strain P7810 of fowl Pasteur's multocidum bacterium, and through steps of anabiosis and passage of the bacterium seed to form seed bacterium liquid, culturing to proliferate, adding gelatin in 1.5 % and cane sugar in 5 %, packing and freeze drying to obtain live vaccine of fowl Pasteur's multocidum bacterium P7810. The live vaccine is fed to fowl for immunizing to prevent fowl cholera, and has immunizing period as long as 6 months, high protecting rate, low cost and other advantages.