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38 results about "Meloidogyne arenaria" patented technology

Meloidogyne arenaria is a species of plant pathogenic nematodes. This nematode is also known as the peanut root knot nematode. The word "Meloidogyne" is derived from two Greek words that mean "apple-shaped" and "female". The peanut root knot nematode, M. arenaria is one of the "major" Meloidogyne species because of its worldwide economic importance. M. arenaria is a predominant nematode species in the United States attacking peanut in Alabama, Florida, Georgia, and Texas. The most damaging nematode species for peanut in the USA is M. arenaria race 1 and losses can exceed 50% in severely infested fields. Among the several Meloidogyne species that have been characterized, M. arenaria is the most variable both morphologically and cytologically. In 1949, two races of this nematode had been identified, race 1 which reproduces on peanut and race 2 which cannot do so. However, in a recent study, three races were described (races 1, 2, and 3). López-Pérez et al (2011) had also studied populations of M. arenaria race 2, which reproduces on tomato plants carrying the Mi gene and race 3, which reproduces on both resistant pepper and tomato.

Materials and methods for in vitro production of bacteria

The subject invention provides novel and advantageous methods for growing bacteria. The methods of the subject invention are particularly advantageous for growing parasitic bacteria in vitro, without the presence of host tissue. In one embodiment of the subject invention, Pasteuria endospores, such as those that infect the rootknot nematode Meloidogyne arenaria or other host nematodes, are grown in vitro under acidic conditions. The process of the subject invention is highly advantageous because Pasteuria can be grown in the absence of nematode tissue.
Owner:PASTEURIA BIOSCI +1

Materials and methods for the efficient production of Pasteuria

The subject invention provides novel and advantageous methods for growing bacteria. The methods of the subject invention are particularly advantageous for growing parasitic bacteria, in vitro, without the presence of host tissue. In one embodiment of the subject invention, Pasteuria spores, such as those that infect the rootknot nematode Meloidogyne arenaria or other host nematodes, are grown in vitro. The process of the subject invention is highly advantageous because Pasteuria can be grown in the absence of nematode tissue.
Owner:SYNGENTA PARTICIPATIONS AG +1

Preparation method of streptomyces having activity for poisoning plant nematodes and uses thereof

InactiveCN101225370ABiocideBacteriaOrder ActinomycetalesPlant nematode
The invention relates to a culturing method of streptomyces with the function of poisoning plant menatodes and a preparation method and application of the metabolin, belonging to the technical field of microbial pesticides. The streptomyces bacterial strain Snea253 is Streptomyces venezuelae, stored in China General Microbiological Culture Collection Center on January 18, 2008 (address: Datun Road, Zhaoyang District, Beijing; post code: 100101) with CGMCC No.2348. The streptomyces bacterial strain Snea253 is classified in species Streptomyces venezuelae, genus streptomyces, order actinomycetales, class actinomycetales, phylum actinomycetales. The culturing method of streptomyces with the function of poisoning plant menatode has the advantages that: through liquid fermentation culture preparation, the metabolites can poison plant parasitic nematodes, in particular Heterodera glycines, Meloidogyne spp. and Aphelenchoides besseyi with distinct effects; the fermentation liquid of the bacterial strain has higher poisonous activity to second instar larva of Heterodera glycines, and can remarkably restrain cyst hatching; the treatment of fermentation liquid with different diluted concentration is significantly different from the treatment of sterile water; the insecticidal effects of stock fermentation solution can reach above 90%, and the effects of greenhouse experiment are obvious; at the same time, the bacterial strain can remarkably poison the Meloidogyne spp. and Aphelenchoides besseyi with good application and development prospects.
Owner:SHENYANG AGRI UNIV

Methods for coupling resistance alleles in tomato

A Lycopersicon esculentum plant comprising within its genome at least one tomato yellow leaf curl virus (TYLCV) resistance allele and at least one root knot nematode resistance allele, characterized in that the resistance alleles are present in coupling phase at different loci on one chromosome and in that the plant is highly resistant both against TYLCV and to at least one root knot nematode species selected from the group consisting of Meloidogyne arenaria, Meloidogyne incognita and Meloidogyne javanica.
Owner:SEMINIS VEGETABLE SEEDS INC

Biological medicament for preventing and treating plant parasitic nematode and uses thereof

The invention discloses a biological medicine for preventing and curing plant parasitic nematode and application thereof. The active ingredient of the biological medicine is Streptomyces rubrogriseus HDZ-9-47 CGMCC No 2878. The plant parasitic nematode is prevented and cured by using the biological medicine in the invention, and the results show that the Streptomyces rubrogriseus HDZ-9-47 CGMCC No 2878 and the metabolite thereof have very strong toxic activity for eggs of Meloidogyne incognita and can obviously inhibit hatching of eggs, and have obvious parasitization for eggs and egg capsules of Meloidogyne incognita; when Streptomyces rubrogriseus HDZ-9-47 CGMCC No 2878 and the metabolite thereof are mixedly used, the effect of insect disinfestation on Meloidogyne incognita can reach 100%. The greenhouse experimental results of tomatoes show that the Streptomyces rubrogriseus HDZ-9-47 CGMCC No. 2878 has very good effect of killing nematode. The biological medicine in the invention has good application development prospect for prevention and cure of plant parasitic nematode.
Owner:CHINA AGRI UNIV

Method for preparing transplanting matrix for blueberry pot seedlings

The invention discloses a method for preparing a transplanting matrix for blueberry pot seedlings. The method comprises the following steps: (1) surveying the soil pH of a seedling raising field; (2) deeply plowing the seedling raising field by the depth of 30-40cm, and removing foreign bodies such as stone; (3) applying sulfur powder to the seedling raising field so as to adjust the soil pH value to an appropriate pH value, meanwhile, broadcasting dazomet to the seedling raising field, carrying out uniform stirring, and then, covering the seedling raising field with films for fumigation; (4) adding peat and fermented sawdust which are in a certain ratio into soil, so as to improve the soil and increase the organic matter content of the soil; and (5) carrying out uniform stirring mechanically, so as to obtain a mixture for later use. According to the method, the dazomet is adopted to fumigate the soil, so that the disease damage caused by meloidogyne spp. is avoided, and the cost for treating weeds is reduced; meanwhile, the soil pH value is adjusted by using the sulfur powder, and a synergetic effect of the dazomet and the sulfur is generated, so as to obtain a better effect; and the fermented sawdust and the peat are added and interact with the sulfur, so that the structural formation of soil aggregates is promoted, the organic matter content is increased, the quality of the soil is improved, and the success rate of blueberry seedling raising is greatly increased.
Owner:青岛沃林蓝莓果业有限公司

Primers and method for directly and simultaneously detecting Meloidogyne and Rotylenchulus reniformis in soil

The invention discloses primers and a method for directly and simultaneously detecting Meloidogyne and Rotylenchulus reniformis in soil and belongs to the technical field of molecular biology detection. Nucleotide sequences of the primers for directly and simultaneously detecting the Meloidogyne and the Rotylenchulus reniformis in the soil are shown as SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5 and SEQ ID NO:6 respectively. The invention also discloses the method for directly and simultaneously detecting the Meloidogyne and the Rotylenchulus reniformis in the soil. The method comprises the following steps of: directly extracting deoxyribonucleic acid (DNA) from soil, purifying, performing first-round polymerase chain reaction (PCR) by using the universal primers of nematodes, namely D2A and D3B by taking the purified DNA as a template, performing second-round PCR by using the primers which are shown as SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5 and SEQ ID NO:6 by taking a product as a template, and judging whether the Meloidogyne and the Rotylenchulus reniformis are contained in a sample through the magnitude of fragments of a PCR product. The primers are sensitive and high in specificity; by the method for directly and simultaneously detecting the Meloidogyne and the Rotylenchulus reniformis in the soil, the detection time can be greatly saved, and results are accurate and reliable; and the primers have high practical application value in the aspect of the rapid detection of the Meloidogyne and the Rotylenchulus reniformis.
Owner:嘉兴卓十生物科技有限公司

Pesticide composition containing dazomet and 1,3-dichloropropylene for soil fumigation

The invention relates to wettable powder, powder or a microgranule preparation prepared from accessory ingredients, such as a wetting agent, a dispersing agent, fillers and the like, and a pesticide composition containing dazomet and 1,3-dichloropropylene for controlling soil-borne diseases such as Phytoph-thora capsici Leonian and the like as well as Meloidogyne. The invention also discloses a method for preventing the soil-borne diseases such as the Phytoph-thora capsici Leonian and the like as well as Meloidogyne by using the composition containing the dazomet and the 1,3-dichloropropylene. The dazomet and the 1,3-dichloropropylene are mixed at the ratio of (1:2)-(2:1) to especially perform action on the Phytoph-thora capsici Leonian and the Meloidogyne. The pesticide composition is not only used for controlling the Phytoph-thora capsici Leonian and the Meloidogyne, but also widely applied to the aspects of controlling the soil-borne diseases (including the meloidogyne) in vegetables, such as hot peppers, gingers, cucumbers and the like, field crops and flowers. The pesticide composition has high application value and obvious economic and social benefits.
Owner:SHANDONG AGRICULTURAL UNIVERSITY

Preparation for prevention and treatment of tobacco and tomato meloidogyne spp. and application thereof

The invention discloses a preparation for prevention and treatment of tobacco and tomato meloidogyne spp. and an application thereof. The preparation for prevention and treatment of tobacco and tomato meloidogyne spp. has an active ingredient of a fermentation liquid of a strain of bacillus amyloliquefaciens YN2010-KC CGMCC No.5722. The preparation not only is a microbial fertilizer which can significantly promote the growth of plants, but also is a biological pesticide and has the average relative control efficiency on tobacco meloidogyne spp. disease of up to 78.32%; the preparation has the control efficiency on the tomato meloidogyne spp. disease of up to 74.50% or more, and has remarkable prevention and treatment effect. The preparation has low production cost, an application method is simple and easy to implement, and the preparation can be used for direct seed dressing and root irrigation, has no toxicity, no pollution and no residue, and is safe to human and livestock.
Owner:MICROBIAL FERMENTATION ENG RES CENT CO LTD OF YUNNAN PROVINCE

Materials and methods for in vitro production of bacteria

The subject invention provides novel and advantageous methods for growing bacteria. The methods of the subject invention are particularly advantageous for growing parasitic bacteria in vitro, without the presence of host tissue. In one embodiment of the subject invention, Pasteuria endospores, such as those that infect the rootknot nematode Meloidogyne arenaria or other host nematodes, are grown in vitro under acidic conditions. The process of the subject invention is highly advantageous because Pasteuria can be grown in the absence of nematode tissue.
Owner:PASTEURIA BIOSCI +1

Pesticide for preventing and killing soil nematode and other pests and its prepn

The present invention discloses one kind of pesticide for preventing and killing soil nematode and other pests and its preparation. Emamectin benzoate as main material is mixed with solvent, emulsifier and penetrant to form the pesticide oil cream. The pesticide oil cream is diluted and irradiated to roots or sprayed to soil so as to prevent and controlling meloidogyne hapla and other pests. It has simple use, low consumption, high pesticidal effect, fast degradation and no residue, and may be used in produce green food material.
Owner:领先生物农业股份有限公司

Preparation method and application of bacillus methylotrophilus LW-6 bacterial agent for biological control of meloidogyne

The invention discloses a preparation method and application of a bacillus methylotrophilus LW-6 bacterial agent for biological control of meloidogyne. The bacillus methylotrophilus LW-6 has the advantages of strong stress resistance, high-temperature resistance, drought resistance, and fast growth and reproduction, and has the characteristics of strong colonization and competitiveness, an obviousnematicidal effect, no toxicity to humans and animals and environmental friendliness. The viable spores and secondary metabolites of the bacillus methylotrophilus LW-6 have the functions of preventing and controlling tobacco meloidogyne and tobacco growth promotion, the efficiency of preventing and controlling the meloidogyne by drip irrigation treatment can reach 70.79%, and the meloidogyne killing ratio can reach 93%. The number of viable spores of a liquid microbial agent prepared by the steps of plate-activated culture of the bacillus methylotrophilus LW-6, seed culture and fermentation can be up to 5 billion / ml, and the preparation method specially comprises the following steps: preferred plate culture, specific seed culture, preferred fermentation, ultrafiltration concentration, low-temperature drying, and dosage form processing preparation.
Owner:陕西恒田生物农业有限公司

Application of benzoic acid in prevention and treatment of plant nematode diseases

The invention relates to the technical field of prevention and treatment of plant diseases, in particular to application of benzoic acid in prevention and treatment of plant nematode diseases, wherein the benzoic acid is used as an effective component in nematicide. Based on the research result of the invention, the benzoic acid has strong toxic activity on various nematodes such as Meloidogyne, Heterodera, Bursaphelenchusxylophilus and the like, can be used for effectively preventing and treating plant nematode diseases, has the advantages of low harm to a human body, low toxicity, low residue, easiness in degradation, greenness, environmental protection and the like, and has good application values are realized in the aspects of preventing and controlling the plant nematodes and developing related medicament products.
Owner:SOUTH CHINA AGRI UNIV +1

Purpureocillium lilacinum capable of preventing and treating Meloidogyne and application thereof

The invention provides an isolated Purpureocillium lilacinum strain, a composition containing the same, and an application of the strain in prevention and treatment of Meloidogyne. The strain is highin sporulation capacity, high in viable count and good in prevention and treatment effect, and can be used for prevention and treatment of various plants.
Owner:MOON (GUANGZHOU) BIOTECH CO LTD

Pesticide applying control method for susceptible diseases of Mammoth gold under acid soil condition

The invention discloses a pesticide applying control method for susceptible diseases of Mammoth gold under acid soil condition, and belongs to the technical field of tobacco planting. The pesticide applying control method comprises the steps of improving soil with quick lime at the stage of preparing soil for ploughing and sowing, during simultaneous control, and simultaneously applying medicamentfor controlling phytophthora parasitica var. nicotianae and rhizoctonia solani Kuhn and medicament for controlling meloidogyne spp. to lands for growing field crops in different growing stages. Special control on fusarium axysporum is performed from the stage of placing plates to rosette stage. During alternative control, medicament for controlling alterna ria alterna ta and medicament for controlling Pseudomonas syringae pv. Tacaci are alternatively applied to the lands for growing field crops from the vigorous growing stage, wherein the time intervals for applying the medicament each time is 7-10 days. According to control risk degrees, the number of applying numbers of the control medicament is adjusted during simultaneous control, special control and alternative control, and accordingto the disease generation kinds, the kinds and the applying manners of the control medicaments can be adjusted in the total growth stage of the tobacco plants. The medicament applying control methodcan perform pinpoint control on various susceptible diseases in the planting process of the Mammoth gold, and the effect is notable.
Owner:YUNNAN TOBACCO CO LTD KUNMING BRANCH +1

Meloidogyne culture medium and culture method

The invention discloses a meloidogyne culture medium. The meloidogyne culture medium comprises compound amino acid powder, fish meal, calcium chloride dihydrate, agar powder and water. By adding the compound amino acid powder and the fish meal into the water agar culture medium, the yield of the meloidogyne can be greatly increased, and the period is shortened, so that the invention provides a simple, convenient and rapid culture method capable of obtaining a large number of plant meloidogyne, and the problems of dependence on hosts, tedious operation, long culture time and small meloidogyne number in the existing meloidogyne culture are solved.
Owner:广东博沃特生物技术有限公司

Marigold straw organic fertilizer with nematode control function and preparation method thereof

The invention discloses a marigold straw organic fertilizer with a nematode control function and a preparation method thereof. The marigold straw organic fertilizer is prepared from the following rawmaterials in parts by weight: 800-900 parts of marigold straw, 80-150 parts of rape cake, 0.2-0.5 part of a decomposing inoculant, 15-30 parts of ammonium nitrate, 0.2-0.5 part of potassium phosphiteand 4.6-19 parts of potassium silicate, wherein the decomposing inoculant is prepared from trichoderma viride, aspergillus niger and bacillus subtilis in an equal mass ratio; the organic fertilizer provided by the invention can effectively improve the conversion of nematocidal active ingredients through secondary fermentation, and enhance the reduction rate of meloidogyne in soil.
Owner:MICROBIAL FERMENTATION ENG RES CENT CO LTD OF YUNNAN PROVINCE +1

Quasi-trichoderma harzianum strain and application thereof

The invention discloses a quasi-trichoderma harzianum strain and an application thereof. The stain is quasi-trichoderma harzianum strain Thar2 which is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC NO.10924. For the application of a biocontrol seed coating agent prepared from the quasi-trichoderma harzianum strain Thar2 in prevention and treatment of Meloidogyne, the quasi-trichoderma harzianum Thar2 strain is a fungus obtained by being separated from soil, is harmless to crops, has prevention and treatment functions for the Meloidogyne, has safety in the application and is suitable for biological control of the Meloidogyne. The death rate of quasi-trichoderma harzianum Thar2 strain fermentation broth on second instar larvae of the Meloidogyne reaches over 98.8 percent, the prevention and treatment efficiency in a greenhouse reaches 83.5 percent, the prevention and treatment efficiency is high and stable, and the quasi-trichoderma harzianum Thar2 strain fermentation broth is suitable for agricultural production requirements.
Owner:MOON (GUANGZHOU) BIOTECH CO LTD

Composite biological preparation and application thereof in elimination of meloidogyne

The invention relates to a composite biological preparation and application thereof in elimination of meloidogyne. The active component contained in the composite biological preparation is a nematodecontrol composition, which contains algae polysaccharide, xylanase, cellulase, protease and beta-glucosidase. The composite biological preparation prepared with the composition is soluble in water, can be added into irrigation liquid and seep into the soil at the same time of irrigation, thus achieving the meloidogyne control effect, the composite biological preparation also can be applied to irrigation treatment on soil before plant sowing, and the use method is simple and effective. The composite biological preparation can reach a nematode control rate up to 90% or more, also is non-toxic and harmless to animals and plants, and is low in cost, thus being suitable for mass production and promotion.
Owner:北京国康本草物种生物科学技术研究院有限公司

Preparation method of streptomyces having activity for poisoning plant nematodes and uses thereof

InactiveCN101225370BBiocideBacteriaBiotechnologyOrder Actinomycetales
The invention relates to a culturing method of streptomyces with the function of poisoning plant menatodes and a preparation method and application of the metabolin, belonging to the technical field of microbial pesticides. The streptomyces bacterial strain Snea253 is Streptomyces venezuelae, stored in China General Microbiological Culture Collection Center on January 18, 2008 (address: Datun Road, Zhaoyang District, Beijing; post code: 100101) with CGMCC No.2348. The streptomyces bacterial strain Snea253 is classified in species Streptomyces venezuelae, genus streptomyces, order actinomycetales, class actinomycetales, phylum actinomycetales. The culturing method of streptomyces with the function of poisoning plant menatode has the advantages that: through liquid fermentation culture preparation, the metabolites can poison plant parasitic nematodes, in particular Heterodera glycines, Meloidogyne spp. and Aphelenchoides besseyi with distinct effects; the fermentation liquid of the bacterial strain has higher poisonous activity to second instar larva of Heterodera glycines, and can remarkably restrain cyst hatching; the treatment of fermentation liquid with different diluted concentration is significantly different from the treatment of sterile water; the insecticidal effects of stock fermentation solution can reach above 90%, and the effects of greenhouse experiment are obvious; at the same time, the bacterial strain can remarkably poison the Meloidogyne spp. and Aphelenchoides besseyi with good application and development prospects.
Owner:SHENYANG AGRI UNIV

Primers and method for directly and simultaneously detecting Meloidogyne and Rotylenchulus reniformis in soil

The invention discloses primers and a method for directly and simultaneously detecting Meloidogyne and Rotylenchulus reniformis in soil and belongs to the technical field of molecular biology detection. Nucleotide sequences of the primers for directly and simultaneously detecting the Meloidogyne and the Rotylenchulus reniformis in the soil are shown as SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5 and SEQ ID NO:6 respectively. The invention also discloses the method for directly and simultaneously detecting the Meloidogyne and the Rotylenchulus reniformis in the soil. The method comprises the following steps of: directly extracting deoxyribonucleic acid (DNA) from soil, purifying, performing first-round polymerase chain reaction (PCR) by using the universal primers of nematodes, namely D2A and D3B by taking the purified DNA as a template, performing second-round PCR by using the primers which are shown as SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5 and SEQ ID NO:6 by taking a product as a template, and judging whether the Meloidogyne and the Rotylenchulus reniformis are contained in a sample through the magnitude of fragments of a PCR product. The primers are sensitive and high in specificity; by the method for directly and simultaneously detecting the Meloidogyne and the Rotylenchulus reniformis in the soil, the detection time can be greatly saved, and results are accurate and reliable; and the primers have high practical application value in the aspect of the rapid detection of the Meloidogyne and the Rotylenchulus reniformis.
Owner:嘉兴卓十生物科技有限公司

Separation method of meloidogyne incognita eggs

PendingCN114176044AHigh egg activityAnimal husbandryAnimal sciencePlant roots
The invention discloses a method for separating meloidogyne incognita egg granules, which comprises the following steps of: performing static incubation, NaClO dissociation, oscillation, centrifugation, cleaning and the like on meloidogyne incognita egg blocks stripped from the surface of a plant root system, so that pure meloidogyne incognita egg granules can be separated, meanwhile, higher egg activity is ensured, and the method has popularization and application values.
Owner:陕西省生物农业研究所

Method for preventing and treating fruit and vegetable meloidogyne disease

The invention discloses a method for preventing and treating fruit and vegetable meloidogyne disease. The method comprises the following steps: treating fruit and vegetable vegetables by using a meloidogyne disease treating powder and paecilomyces lilacinus; spreading meloidogyne treating powder based on 2-4kg / mu as base fertilizer to the land during land preparation; performing rotary tillage, bedding, film covering, holing and field planting; dropwise irrigating a paecilomyces lilacinus aqueous solution after seedlings of the fruit and vegetable slowly grow once every 20-30 days based on 1000-2000ml / mu; and stopping dropwise irrigating 7d before uprooting. With the adoption of the method, the fruit and vegetable meloidogyne disease can be effectively prevented and treated, and the preventing and treating effect reaches 89.07-95.13%.
Owner:CHIFENG UNIV

Bacillus aerophilus and application of bacillus aerophilus in killing meloidogyne

ActiveCN107164290AFast growthHigh sporulationBiocideBacteriaBiotechnologyBacillus aerophilus
The invention provides Bacillus aerophilus ZDXC-01. The collection number of Bacillus aerophilus ZDXC-01 is CGMCC (China general microbiological culture collection center) No.14104. The invention further provides a 16S rRNA gene segment of the strain, a fermentation liquid, a fermentation method, an application of the strain in prevention of meloidogyne and the like.
Owner:CHINA KINGDOM AGRITECH QINGDAO

New uses of calcium formate in prevention and control of Meloidogyne incognita

The present invention provides new uses of calcium formate in prevention and control of Meloidogyne incognita, particularly new uses of calcium formate in preparation of a Meloidogyne incognita insecticide, wherein calcium formate can be separately used as an active component, and can further be compatible with other active components capable of preventing and controlling Meloidogyne incognita so as to be co-used as an active component, the Meloidogyne incognita insecticide is a calcium formate solution, the advantages of safety, no toxicity and effectiveness can be provided by using the calcium formate solution to prevent and control Meloidogyne incognita, the Meloidogyne incognita egg hatching inhibition rate can achieve 90% when the concentration of the calcium formate solution is 8.00-10.00 mM / L, and the Meloidogyne incognita second instar larva fatality rate is 30-40% when the concentration of the calcium formate solution is 8.00-10.00 mM / L.
Owner:CHINA AGRI UNIV

A kind of preparation method of blueberry bowl seedling transplanting matrix

The invention discloses a method for preparing a transplanting matrix for blueberry pot seedlings. The method comprises the following steps: (1) surveying the soil pH of a seedling raising field; (2) deeply plowing the seedling raising field by the depth of 30-40cm, and removing foreign bodies such as stone; (3) applying sulfur powder to the seedling raising field so as to adjust the soil pH value to an appropriate pH value, meanwhile, broadcasting dazomet to the seedling raising field, carrying out uniform stirring, and then, covering the seedling raising field with films for fumigation; (4) adding peat and fermented sawdust which are in a certain ratio into soil, so as to improve the soil and increase the organic matter content of the soil; and (5) carrying out uniform stirring mechanically, so as to obtain a mixture for later use. According to the method, the dazomet is adopted to fumigate the soil, so that the disease damage caused by meloidogyne spp. is avoided, and the cost for treating weeds is reduced; meanwhile, the soil pH value is adjusted by using the sulfur powder, and a synergetic effect of the dazomet and the sulfur is generated, so as to obtain a better effect; and the fermented sawdust and the peat are added and interact with the sulfur, so that the structural formation of soil aggregates is promoted, the organic matter content is increased, the quality of the soil is improved, and the success rate of blueberry seedling raising is greatly increased.
Owner:青岛沃林蓝莓果业有限公司

Trichoderma epiphyte having nematicidal activity as well as preparation method and uses thereof

The invention relates to a fungi culture method, which has toxic action on plant parasitic nematode. The invention also relates to a metabolite preparation method of the fungi and the application, which belongs to the technology area of microbial pesticide. The Trichoderma spp strain Snef85 related to the invention is Trichoderma harzianum, which is stored in General Microbiological Culture Collection Center (address: Datun Road, Chaoyang District, Beijing, China, 100101) on 30 October, 2007. And the preservation number of the strain is CGMCC No: M2235. The taxonomic status of Trichoderma sppstrain Snef85 is Deuteromycotina, Hyphomycetales, Moniliales, Moniliaceae, Trichoderma, Harzianum. The fungi is prepared through liquid fermentation culture method; the metabolite has the outstandingcharacteristics of strong toxic action and significant nematode killing effect on plant parasitic nematode, such as Meloidogyne spp., Aphelenchoides besseyi and Heterodera glycines especially. The fermentation broth of the strain has excellent control effect on Meloidogyne spp., Aphelenchoides besseyi and Heterodera glycines. Significant differences were observed between treatment with sterilizedwater and with Aspergillus fermentation broth at different concentrations. The nematode killing effect of fermentation broth can reach more than 95 percent. The invention has great application and development prospect.
Owner:SHENYANG AGRI UNIV

Method for detecting single root knot of enterolobium cyclocarpum meloidogyne and application thereof

The invention discloses a method for detecting a single knot of enterolobium cyclocarpum meloidogyne and application thereof. In the invention, universal primers MF (Forward Primer) and MR (Reverse Primer) for amplifying meloidogyne and specific primers FMe (Forward Primer) and RMe (Reverse Primer) for amplifying the enterolobium cyclocarpum meloidogyne are designed; a single root knot DNA containing the meloidogyne is taken as a template for PCR (Polymerase Chain Reaction) amplification reaction; a PCR amplification reaction product is subjected to lipolysaccharide electrophoresis detection, and a detection result is obtained according to the existence / absence and the size of straps; and the sequences of the primers are respectively shown as SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3 and SEQ ID NO:4 in a table. The one-step dual PCR detection method disclosed by the invention has the advantages of increased molecular detection speed, increased sensitivity and accuracy, and has a practical application value in the aspect of detecting the enterolobium cyclocarpum meloidogyne.
Owner:嘉兴卓十生物科技有限公司

EM bacteria cultured by ascophyllum nodosum enzymatic hydrolysate and application of EM bacteria to prevention and treatment of meloidogyne

The invention relates to the technical field of microorganisms, and particularly provides ascophyllum nodosum enzymatic hydrolysate, a process for culturing EM bacteria by using the ascophyllum nodosum enzymatic hydrolysate as a culture medium component, and an application of the cultured EM bacteria to prevention and treatment of meloidogyne. The ascophyllum nodosum enzymatic hydrolysate is obtained by performing enzymolysis on ascophyllum nodosum by using ADL compound enzyme, and can be used as an important component to be added into a culture medium for culturing the EM bacteria, so that stable and high-yield viable bacteria can be obtained. Research on obtained fermentation liquid shows that the fermentation liquid has an extremely remarkable prevention and control effect on M.incognita, a new direction is provided for prevention and control of the meloidogyne, and the fermentation liquid better serves agricultural production.
Owner:山东科大创业生物有限公司
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