38 results about "Meloidogyne arenaria" patented technology

The subject invention provides novel and advantageous methods for growing bacteria. The methods of the subject invention are particularly advantageous for growing parasitic bacteria in vitro, without the presence of host tissue. In one embodiment of the subject invention, Pasteuria endospores, such as those that infect the rootknot nematode Meloidogyne arenaria or other host nematodes, are grown in vitro under acidic conditions. The process of the subject invention is highly advantageous because Pasteuria can be grown in the absence of nematode tissue.

The invention discloses a preparation method and application of a bacillus methylotrophilus LW-6 bacterial agent for biological control of meloidogyne. The bacillus methylotrophilus LW-6 has the advantages of strong stress resistance, high-temperature resistance, drought resistance, and fast growth and reproduction, and has the characteristics of strong colonization and competitiveness, an obviousnematicidal effect, no toxicity to humans and animals and environmental friendliness. The viable spores and secondary metabolites of the bacillus methylotrophilus LW-6 have the functions of preventing and controlling tobacco meloidogyne and tobacco growth promotion, the efficiency of preventing and controlling the meloidogyne by drip irrigation treatment can reach 70.79%, and the meloidogyne killing ratio can reach 93%. The number of viable spores of a liquid microbial agent prepared by the steps of plate-activated culture of the bacillus methylotrophilus LW-6, seed culture and fermentation can be up to 5 billion / ml, and the preparation method specially comprises the following steps: preferred plate culture, specific seed culture, preferred fermentation, ultrafiltration concentration, low-temperature drying, and dosage form processing preparation.

The invention discloses a pesticide applying control method for susceptible diseases of Mammoth gold under acid soil condition, and belongs to the technical field of tobacco planting. The pesticide applying control method comprises the steps of improving soil with quick lime at the stage of preparing soil for ploughing and sowing, during simultaneous control, and simultaneously applying medicamentfor controlling phytophthora parasitica var. nicotianae and rhizoctonia solani Kuhn and medicament for controlling meloidogyne spp. to lands for growing field crops in different growing stages. Special control on fusarium axysporum is performed from the stage of placing plates to rosette stage. During alternative control, medicament for controlling alterna ria alterna ta and medicament for controlling Pseudomonas syringae pv. Tacaci are alternatively applied to the lands for growing field crops from the vigorous growing stage, wherein the time intervals for applying the medicament each time is 7-10 days. According to control risk degrees, the number of applying numbers of the control medicament is adjusted during simultaneous control, special control and alternative control, and accordingto the disease generation kinds, the kinds and the applying manners of the control medicaments can be adjusted in the total growth stage of the tobacco plants. The medicament applying control methodcan perform pinpoint control on various susceptible diseases in the planting process of the Mammoth gold, and the effect is notable.

The invention relates to a culturing method of streptomyces with the function of poisoning plant menatodes and a preparation method and application of the metabolin, belonging to the technical field of microbial pesticides. The streptomyces bacterial strain Snea253 is Streptomyces venezuelae, stored in China General Microbiological Culture Collection Center on January 18, 2008 (address: Datun Road, Zhaoyang District, Beijing; post code: 100101) with CGMCC No.2348. The streptomyces bacterial strain Snea253 is classified in species Streptomyces venezuelae, genus streptomyces, order actinomycetales, class actinomycetales, phylum actinomycetales. The culturing method of streptomyces with the function of poisoning plant menatode has the advantages that: through liquid fermentation culture preparation, the metabolites can poison plant parasitic nematodes, in particular Heterodera glycines, Meloidogyne spp. and Aphelenchoides besseyi with distinct effects; the fermentation liquid of the bacterial strain has higher poisonous activity to second instar larva of Heterodera glycines, and can remarkably restrain cyst hatching; the treatment of fermentation liquid with different diluted concentration is significantly different from the treatment of sterile water; the insecticidal effects of stock fermentation solution can reach above 90%, and the effects of greenhouse experiment are obvious; at the same time, the bacterial strain can remarkably poison the Meloidogyne spp. and Aphelenchoides besseyi with good application and development prospects.

The invention discloses primers and a method for directly and simultaneously detecting Meloidogyne and Rotylenchulus reniformis in soil and belongs to the technical field of molecular biology detection. Nucleotide sequences of the primers for directly and simultaneously detecting the Meloidogyne and the Rotylenchulus reniformis in the soil are shown as SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5 and SEQ ID NO:6 respectively. The invention also discloses the method for directly and simultaneously detecting the Meloidogyne and the Rotylenchulus reniformis in the soil. The method comprises the following steps of: directly extracting deoxyribonucleic acid (DNA) from soil, purifying, performing first-round polymerase chain reaction (PCR) by using the universal primers of nematodes, namely D2A and D3B by taking the purified DNA as a template, performing second-round PCR by using the primers which are shown as SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5 and SEQ ID NO:6 by taking a product as a template, and judging whether the Meloidogyne and the Rotylenchulus reniformis are contained in a sample through the magnitude of fragments of a PCR product. The primers are sensitive and high in specificity; by the method for directly and simultaneously detecting the Meloidogyne and the Rotylenchulus reniformis in the soil, the detection time can be greatly saved, and results are accurate and reliable; and the primers have high practical application value in the aspect of the rapid detection of the Meloidogyne and the Rotylenchulus reniformis.

The invention discloses a method for preparing a transplanting matrix for blueberry pot seedlings. The method comprises the following steps: (1) surveying the soil pH of a seedling raising field; (2) deeply plowing the seedling raising field by the depth of 30-40cm, and removing foreign bodies such as stone; (3) applying sulfur powder to the seedling raising field so as to adjust the soil pH value to an appropriate pH value, meanwhile, broadcasting dazomet to the seedling raising field, carrying out uniform stirring, and then, covering the seedling raising field with films for fumigation; (4) adding peat and fermented sawdust which are in a certain ratio into soil, so as to improve the soil and increase the organic matter content of the soil; and (5) carrying out uniform stirring mechanically, so as to obtain a mixture for later use. According to the method, the dazomet is adopted to fumigate the soil, so that the disease damage caused by meloidogyne spp. is avoided, and the cost for treating weeds is reduced; meanwhile, the soil pH value is adjusted by using the sulfur powder, and a synergetic effect of the dazomet and the sulfur is generated, so as to obtain a better effect; and the fermented sawdust and the peat are added and interact with the sulfur, so that the structural formation of soil aggregates is promoted, the organic matter content is increased, the quality of the soil is improved, and the success rate of blueberry seedling raising is greatly increased.

A new and distinct tomato variety NUN 00318 TOP is disclosed, as well as seeds and plants and fruits thereof. NUN 00318 TOP is a determinate tomato variety of the Brix type with extended field holding capacity for the processing market, comprising resistance to Verticillium dahliae, Fusarium oxysporum s.p. lycopersici Race 2 (ex 3), Meloidogyne incognita, and Tomato Spotted Wilt Virus (TSWV).

The invention relates to a fungi culture method, which has toxic action on plant parasitic nematode. The invention also relates to a metabolite preparation method of the fungi and the application, which belongs to the technology area of microbial pesticide. The Trichoderma spp strain Snef85 related to the invention is Trichoderma harzianum, which is stored in General Microbiological Culture Collection Center (address: Datun Road, Chaoyang District, Beijing, China, 100101) on 30 October, 2007. And the preservation number of the strain is CGMCC No: M2235. The taxonomic status of Trichoderma sppstrain Snef85 is Deuteromycotina, Hyphomycetales, Moniliales, Moniliaceae, Trichoderma, Harzianum. The fungi is prepared through liquid fermentation culture method; the metabolite has the outstandingcharacteristics of strong toxic action and significant nematode killing effect on plant parasitic nematode, such as Meloidogyne spp., Aphelenchoides besseyi and Heterodera glycines especially. The fermentation broth of the strain has excellent control effect on Meloidogyne spp., Aphelenchoides besseyi and Heterodera glycines. Significant differences were observed between treatment with sterilizedwater and with Aspergillus fermentation broth at different concentrations. The nematode killing effect of fermentation broth can reach more than 95 percent. The invention has great application and development prospect.

The invention discloses a method for detecting a single knot of enterolobium cyclocarpum meloidogyne and application thereof. In the invention, universal primers MF (Forward Primer) and MR (Reverse Primer) for amplifying meloidogyne and specific primers FMe (Forward Primer) and RMe (Reverse Primer) for amplifying the enterolobium cyclocarpum meloidogyne are designed; a single root knot DNA containing the meloidogyne is taken as a template for PCR (Polymerase Chain Reaction) amplification reaction; a PCR amplification reaction product is subjected to lipolysaccharide electrophoresis detection, and a detection result is obtained according to the existence / absence and the size of straps; and the sequences of the primers are respectively shown as SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3 and SEQ ID NO:4 in a table. The one-step dual PCR detection method disclosed by the invention has the advantages of increased molecular detection speed, increased sensitivity and accuracy, and has a practical application value in the aspect of detecting the enterolobium cyclocarpum meloidogyne.