Trichoderma epiphyte having nematicidal activity as well as preparation method and uses thereof
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A technology for killing nematodes and fungi, which is applied in the field of fungi that poison and kill plant parasitic nematodes and their preparation, and can solve problems such as under-researched
Inactive Publication Date: 2010-06-02
SHENYANG AGRI UNIV
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[0004] In summary, the use of Trichoderma harzianum to biologically control plant-parasitic nematodes has great theoretical research significance and practical application potential, but it has not been studied in depth in the world, especially in my country.
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Embodiment 1
[0019] Embodiment 1: the cultivation of Trichoderma bacterial strain Snef85
[0020] The mycelium of Trichoderma strain Snef85 was inoculated on test tube agar medium, and the medium formula was PDA medium, that is, potato 200g; glucose 20g; agar 17g; distilled water 1000mL. Incubate at 25°C for 10 days to obtain test tube species.
[0021] Then inoculate the test tube into a 100mL Erlenmeyer flask (50mL per bottle) liquid medium (Chape's medium), the medium formula is: NaNO 3 : 2.0g, K 2 HPO 4 : 1.0g, KCl: 0.5g, MgSO 4 ·7H 2 O: 0.5g, FeSO 4 : 0.01g, sucrose: 30.0g; distilled water: 1000mL. At 25°C to 28°C, the shaking table rotates at 150r·min -1 ~200r·min -1 , fermentation 168h. The fermented broth was made into different multiples to carry out the nematicide efficacy test.
Embodiment 2
[0022] Example 2: Nematicidal activity of Trichoderma strain Snef85:
[0023] On the basis of concentrating the 10× solution, the fermentation broth was successively diluted into 6 concentration gradients of 1× (stock solution), 5×, 8×, 10×, 15×, and 20× solution to treat root-knot nematodes (Meloidogyne spp.), Rice stem-point nematode (Aphelenchoides besseyi) and soybean cyst nematode (Heterodera glycines).
[0024] 1. Preparation of preparations for testing
[0025] For the Trichoderma strain Snef85 prepared by the aforementioned liquid fermentation culture method, the nematicidal activity test was carried out with different multiples of the fermentation broth.
[0026] 2. Preparation of Nematodes for Experiments
[0027] Root-knot nematode (Meloidogyne spp.): collected from tomatoes infested by root-knot nematode in the Hunnan area of Shenyang. Take out the tomato root system, rinse it gently with water, carefully remove the oocysts, sterilize it in 1% sodium hypochlorit...
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Abstract
The invention relates to a fungi culture method, which has toxic action on plant parasitic nematode. The invention also relates to a metabolite preparation method of the fungi and the application, which belongs to the technology area of microbial pesticide. The Trichoderma spp strain Snef85 related to the invention is Trichoderma harzianum, which is stored in General Microbiological Culture Collection Center (address: Datun Road, Chaoyang District, Beijing, China, 100101) on 30 October, 2007. And the preservation number of the strain is CGMCC No: M2235. The taxonomic status of Trichoderma sppstrain Snef85 is Deuteromycotina, Hyphomycetales, Moniliales, Moniliaceae, Trichoderma, Harzianum. The fungi is prepared through liquid fermentation culture method; the metabolite has the outstandingcharacteristics of strong toxic action and significant nematode killing effect on plant parasitic nematode, such as Meloidogyne spp., Aphelenchoides besseyi and Heterodera glycines especially. The fermentation broth of the strain has excellent control effect on Meloidogyne spp., Aphelenchoides besseyi and Heterodera glycines. Significant differences were observed between treatment with sterilizedwater and with Aspergillus fermentation broth at different concentrations. The nematode killing effect of fermentation broth can reach more than 95 percent. The invention has great application and development prospect.
Description
Technical field: [0001] The invention belongs to the technical field of microbial pesticides, and in particular relates to a fungus for poisoning and killing plant parasitic nematodes, a preparation method and application thereof. Background technique: [0002] Plant parasitic nematodes occur and cause damage in many countries and regions in the world, and there are various types. On a global scale, the occurrence and harm of plant parasitic nematodes are becoming more and more serious along with the development of agriculture and forestry and the changes of farming and cultivation systems. According to Esser statistics, until 1990, 207 genus and 4832 species of plant parasitic nematodes have been reported to be found in the whole world (Liu Weizhi, Duan Yuxi. 2000. Plant Pathogenic Nematodes. Beijing: China Agricultural Press, p1-2). Plant-parasitic nematodes are estimated to cause an annual loss of 12.3% of the world's major crops, exceeding US$ 100 billion, while actual ...
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