The invention relates to a method for cultivating a
fungus capable of poisoning and killing
plant parasitic nematodes and a method for preparing and using metabolites thereof, belonging to the technical field of microbial pesticides. The
Trichoderma strain Snef85 involved in the present invention is
Trichoderma harzianum, which has been preserved on October 30, 2007 in the General
Microorganism Center of China Committee for the Collection of Microorganisms (address: Datun Road, Chaoyang District, Beijing,
postal code 100101), The deposit number is CGMCC No.2235. The taxonomic status of the
Trichoderma strain Snef85 is Deuteromycotina,
Hyphomycetes, Trichosporales, Trichosporaceae, Trichoderma, and
Trichoderma harzianum. It is prepared by liquid
fermentation culture, and its metabolites have high
toxicity to
plant parasitic nematodes, especially root-knot nematodes incognita, rice stem-point nematodes and soybean
cyst nematodes, and outstanding nematicidal effect. The
fermentation broth of the strain has obvious control effects on root-knot
nematode (
Meloidogynes pp.), rice stem-point
nematode (
Aphelenchoides besseyi) and
soybean cyst nematode (
Heterodera glycines). There are significant differences between the treatment of fermented liquid with different
dilution concentrations and the treatment of aseptic water, and the
nematode killing effect of fermented liquid can reach more than 95%, which has good application and development prospects.