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80 results about "Heterodera" patented technology

Heterodera is a genus of nematodes in the family Heteroderidae. Members of the genus are obligate parasites and different species attack different crops, often causing great economic damage. The genus is unique among nematode genera because of the ability of the female to transform into a tough, brown, cyst which protects the eggs which have been formed within her body. The name heterodera "refers to the different 'skins' of female and cyst."

Nematode-inducible plant gene promoter

An isolated DNA fragment that promotes root knot or cyst nematode indcuible transcription of a coding sequence downstream of and operably linked to the fragment in at least an Arabidopsis thaliana plant and a chimeric DNA sequence including the DNA fragment. Also, methods for the use of the DNA fragment.
Owner:SYNGENTA MOGEN BV

Growth medium moisture replacement system

A growth medium moisture replacement system particularly useful in bioassays for Heterodera glycines-Glycine female development includes a first container of growth medium for soybean cultivars, an enclosed second container for water, and a wick which extends upwardly from the water in the second container into the growth medium in the first container to replace moisture in the growth medium after establishment of a hydraulic gradient. The wick includes a rope member having an impermeable sheath around a middle portion of the rope member, leaving its ends uncovered, and sealed to an opening in the bottom of the first container. Another related growth medium moisture replacement system involves an enclosed container for water having a tube extending downwardly from within the container and sealingly through an opening in the bottom of the container to an end having holes therein, and a wick which extends first upwardly in the container and then downwardly in the tube to an end near the end of the tube to deliver moisture from within the container downwardly into the growth medium in which the tube extends.
Owner:MARYLAND UNIV OF

Soybean sudden death syndrome resistant soybeans, soybean cyst nematode resistant soybeans and methods of breeding and identifying resistant plants

InactiveUS20020129402A1Solves the problem quickly and cheaply selecting resistant cultivarsImprove selection for SDS and SCN resistanceMicrobiological testing/measurementOther foreign material introduction processesBiotechnologyMarker-assisted selection
The invention provides a method of introgressing soybean sudden death syndrome (SDS) or soybean cyst nematode (SCN) resistance into non-resistant soybean germplasm. Loci associated with SDS resistance or with SCN resistance in soybean lines known to be resistant to SDS or to SCN are used in marker assisted selection during introgression of SDS or SCN resistance into elite germplasm. In addition, the method may be used to confirm selection of resistance in new soybean cultivars.
Owner:SOUTHERN ILLINOIS UNIVERSITY

Soybean cultivar BA922834

ActiveUS7304217B1Modifying grain digestibilityModifying nutrient availabilityHorticulture methodsPlant tissue cultureSoybean cyst nematodeHeterodera
A soybean cultivar designated BA922834 with high yield potential, tolerance to Roundup™ herbicide, MG VI maturity, resistance to race 3 of soybean cyst nematode (Heterodera glycines), and resistance to frogeye leaf spot, further including the plants and seeds of the cultivar BA922834, methods for producing a soybean plant by crossing the cultivar BA922834 with itself or another soybean plant. The invention also relates to soybean cultivar BA922834 further comprising one or more single gene traits, and to methods of producing a soybean having such traits by introgression, transformation or mutagenesis. The invention also includes using the soybean cultivar BA922834 to produce other soybean cultivars, breeding lines, and progeny.
Owner:SYNGENTA PARTICIPATIONS AG

Chinese herbal medicinal pesticide and usage method thereof

The invention which provides a Chinese herbal medicinal pesticide and a usage method thereof relates to the pesticide field. The Chinese herbal medicinal seed dressing pesticide is prepared by decocting 470-550g of Radix Euphorbiae Ebractealatae, 460-520g of Stemona sessilifolia, and 460-530g of Sophora flavescens in 18000-25000g of water for 1-3h. The Chinese herbal medicinal seed dressing pesticide is used according to a case that each 1kg of the Chinese herbal medicinal seed dressing pesticide is uniformly stirred with 100-170kg of crop seeds, and then the crops seeds are sown. The Chineseherbal medicinal pesticide of the invention, which has the advantages of reasonable compatibility, low cost and simple preparation method, allows problems of high residual, high pollution and bad pest prevention effect of traditional chemical pesticides to be solved, and simultaneously has control effects on root rot diseases and Heterodera glycines of crops of soybean and the like, and pests of soil grubs, cutworms, borers and the like.
Owner:张志辉

Nematicidal composition and application thereof

The invention discloses a nematicidal composition and application thereof. The composition comprises active ingredients A and B, and a mass ratio of A to B is 20:1 to 1:20. The ingredient A refers to one of fosthiazate, abamectin, furfural, dazomet, fluopyram, carbosulfan, metham, verticillium chlamydosporium, emamectin benzoate, paecilomyces lilacinus or fluensulfone; and the structural formula of the ingredient B is a formula I or a formula II, wherein the formula I is as shown in the specification, and the formula II is as shown in the specification. The nematicidal composition disclosed by the invention is used for killing plant parasite nematodes comprising root-knot nematodes, ditylenchus destructor, heterodera sachachtii, pratylenchus coffeae and bursaphelenchus xylophilus, is composed of a novel nematicide containing lactonic rings and the existing insecticides and bactericides, and has good nematocidal activity and low toxicity to people and livestock. The nematicidal composition disclosed by the invention has an excellent effect of controlling eggs and second-stage larvae of the root-knot nematodes.
Owner:SHANDONG UNITED PESTICIDE IND CO LTD

Trichoderma epiphyte having nematicidal activity as well as preparation method and uses thereof

The invention relates to a method for cultivating a fungus capable of poisoning and killing plant parasitic nematodes and a method for preparing and using metabolites thereof, belonging to the technical field of microbial pesticides. The Trichoderma strain Snef85 involved in the present invention is Trichoderma harzianum, which has been preserved on October 30, 2007 in the General Microorganism Center of China Committee for the Collection of Microorganisms (address: Datun Road, Chaoyang District, Beijing, postal code 100101), The deposit number is CGMCC No.2235. The taxonomic status of the Trichoderma strain Snef85 is Deuteromycotina, Hyphomycetes, Trichosporales, Trichosporaceae, Trichoderma, and Trichoderma harzianum. It is prepared by liquid fermentation culture, and its metabolites have high toxicity to plant parasitic nematodes, especially root-knot nematodes incognita, rice stem-point nematodes and soybean cyst nematodes, and outstanding nematicidal effect. The fermentation broth of the strain has obvious control effects on root-knot nematode (Meloidogynes pp.), rice stem-point nematode (Aphelenchoides besseyi) and soybean cyst nematode (Heterodera glycines). There are significant differences between the treatment of fermented liquid with different dilution concentrations and the treatment of aseptic water, and the nematode killing effect of fermented liquid can reach more than 95%, which has good application and development prospects.
Owner:SHENYANG AGRI UNIV

Soybean cyst nematode resistance gene and application thereof

InactiveCN102206651AEffective guidanceEffectively guide conventional breedingFungiBacteriaGlycineAgricultural science
The invention relates to a soybean cyst nematode resistance gene and application thereof. The cDNA (complementary deoxyribonucleic acid) fragment (SRC-J-6) provided by the invention is derived from soybean (Glycine max (L.) Merrill.) variety L-10, and has a nucleotide sequence shown in 1) a nucleotide sequence shown in SEQ ID NO. 1; 2) a DNA (deoxyribonucleic acid) molecule capable of hybridizingwith the nucleotide sequence shown in SEQ ID NO. 1 and being expressed under strict conditions; 3) an mRNA (messenger ribonucleic acid) molecule, which has homology of more than 90% with the nucleotide sequence shown in 1) and can be expressed; or 4) a DNA (deoxyribonucleic acid) molecule, which has homology of more than 90% with the nucleotide sequence shown in 1) and can be expressed. After theplant tissues are transformed by the gene disclosed by the invention, the resistance to cyst nematode of the transgenic soybean is significantly improved. The soybean cyst nematode resistance gene provided by the invention has important significance, not only can effectively guide conventional breeding, can also provide excellent genetic resources for the transgenic breeding of soybean and has broad application prospects in the soybean production.
Owner:NORTHEAST AGRICULTURAL UNIVERSITY

Natural plant biological composite nutrient solution for killing nematode and preparation method thereof

The invention relates to a natural plant biological composite nutrient solution for killing nematode and a preparation method thereof. The natural plant biological composite nutrient solution for killing nematode comprises bacillus subtilis bacillus subtilis, paecilomyces lilacinus, beauveria bassiana and plants marigold flower, stemona, maidenhair, sophora flavescens, derris trifoliata, melia azedarach and protein liquid according to weight ratio, compared with prior art, the natural plant biological composite nutrient solution has beneficial effects that harm due to soil borne parasites such as root-knot nematode, heterodera sachachtii, and ditylenchus dipsaci can be effectively prevented and treated; no pesticide residue on soil and crops fruits can be realized, usage is convenient, no pollution is generated, nematode harm can be avoided by using the solution for three times in a whole fertility period, and the nutrient solution creates condition for producing green organic food; and has good prevention and treatment effects for crop soil borne disease, can improve the soil and increase soil granular structure, and is capable of preventing soil hardening, increasing the content of the organic matter, dissolving phosphor, releasing potassium and fixating nitrogen, as well as supplementing crop nutrition.
Owner:山西年马农业科技股份有限公司

Preparation method of streptomyces having activity for poisoning plant nematodes and uses thereof

InactiveCN101225370ABiocideBacteriaOrder ActinomycetalesPlant nematode
The invention relates to a culturing method of streptomyces with the function of poisoning plant menatodes and a preparation method and application of the metabolin, belonging to the technical field of microbial pesticides. The streptomyces bacterial strain Snea253 is Streptomyces venezuelae, stored in China General Microbiological Culture Collection Center on January 18, 2008 (address: Datun Road, Zhaoyang District, Beijing; post code: 100101) with CGMCC No.2348. The streptomyces bacterial strain Snea253 is classified in species Streptomyces venezuelae, genus streptomyces, order actinomycetales, class actinomycetales, phylum actinomycetales. The culturing method of streptomyces with the function of poisoning plant menatode has the advantages that: through liquid fermentation culture preparation, the metabolites can poison plant parasitic nematodes, in particular Heterodera glycines, Meloidogyne spp. and Aphelenchoides besseyi with distinct effects; the fermentation liquid of the bacterial strain has higher poisonous activity to second instar larva of Heterodera glycines, and can remarkably restrain cyst hatching; the treatment of fermentation liquid with different diluted concentration is significantly different from the treatment of sterile water; the insecticidal effects of stock fermentation solution can reach above 90%, and the effects of greenhouse experiment are obvious; at the same time, the bacterial strain can remarkably poison the Meloidogyne spp. and Aphelenchoides besseyi with good application and development prospects.
Owner:SHENYANG AGRI UNIV

Soybean cultivar BA947474

A soybean cultivar designated BA947474 with high yield potential, tolerance to Roundup™ herbicide, MG VIII maturity, resistance to race 3 of soybean cyst nematode (Heterodera glycines), and moderate resistance to southern root knot nematode and southern stem canker, further including the plants and seeds of the cultivar BA947474, methods for producing a soybean plant by crossing the cultivar BA947474 with itself or another soybean plant. The invention also relates to soybean cultivar BA947474 further comprising one or more single gene traits, and to methods of producing a soybean having such traits by introgression, transformation or mutagenesis. The invention also includes using the soybean cultivar BA947474 to produce other soybean cultivars, breeding lines, and progeny.
Owner:SYNGENTA PARTICIPATIONS AG

Method for identifying resistance against heterodera glycines

ActiveCN102246662AAchieve duplicate identificationHorticulture methodsBiotechnologyGlycine
The invention relates to a method for identifying resistance against heterodera glycines. The method comprises the following steps: scissoring the tissue of young part of the soybean vegetative part of the susceptible control variety and the variety to be identified to perform inductive rooting culture; after the transplanted seedling survives, inoculating the seedling with heterodera glycines suspension containing 400 eggs and / or second-stage larvae / mL; continuing culture at 20-30 DEG C; when white cyst appears at the seedling root, separating counting the number of root cyst of the susceptible control variety and the variety to be identified; and defining the disease resistance of the material according to the resistance grading standard. The method provided by the invention has the positive and beneficial effects that: as the genotype is not changed when the vegetative body roots, the resistance of the vegetative body can represent the resistance of the parent material; one part of the vegetative body of the material to be identified replaces the entire plant for identification, and the resistive material can be completely stored; and for the population of segregative generation (for example, F2), 3-5 branches on one individual plant can be fetched for identification so as to realize repeated identification of the segregation population.
Owner:HENAN ACAD OF AGRI SCI

Singularity SCAR mark of wheat heterodera avenae sensu lato and rapid PCR detection method thereof

The present invention provides a singularity SCAR mark of a wheat heterodera avenae sensu lato and a rapid PCR detection method thereof, which belongs to the field of biotechnology. DNA fragments of the SCAR mark of the wheat heterodera avenae sensu lato is characterized in that the DNA fragments are provided with a nucleotide sequence shown as SEQ ID NO 1. Auele specific primers HaF1 and HaR1 designed according with the DNA fragments of the singularity SCAR mark of the wheat heterodera avenae sensu lato can amplify a singularity fragment with 1010bp in the PCR rapid detection method for the wheat heterodera avenae sensu lato. According to the Singularity SCAR mark and the detection method, the molecule detection sensitivity is increased and the detection is rapid and accurate, which has a high application valve in the detection aspect of the wheat heterodera avenae sensu lato and the early diagnosis aspect of wheat heterodera glycine.
Owner:INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI

Lecanicillium attenuatum and application of lecanicillium attenuatum to control of crop nematodes and bemisia tabaci

InactiveCN104195050ANormal growthNormal sporulationBiocideFungiSporeMicroorganism
The invention discloses lecanicillium attenuatum and application of the lecanicillium attenuatum to controlling heterodera glycines. The lecanicillium attenuatum is lecanicillium attenuatum HNLA1306, which is preserved in CGMCC (China General Microbiological Culture Collection Center) with the preservation number of CGMCC No.9220. A fermentation liquor of the lecanicillium attenuatum can remarkably restrain incubation of eggs of heterodera glycines and has a strong killing effect on J2 larvas; the conidia of the lecanicillium attenuatum have a good parasitic effect on second-stage juveniles of bemisia tabaci; the lecanicillium attenuatum can be used for biological control of crop nematodes and bemisia tabaci.
Owner:张艳军 +1

Modified Bacillus Thuringiensis Cry6 Proteins For Nematode Control

The subject invention concerns plants protected from nematode feeding damage and improved versions of Cry proteins. The subject invention also concerns improved versions of Cry6A proteins. Synthetic genes encoding these modified proteins are also part of the subject invention. Another embodiment of the subject invention includes plants transformed with the genes of the subject invention. In yet another embodiment the subject invention concerns Bt proteins for in-plant protection against crop damage by root knot nematode (RKN; Meloidogyne incognita) and soybean cyst nematode (SCN; Heterodera glycines).
Owner:DOW AGROSCIENCES LLC

Trans-aconitic acid producing bacteria and application thereof

The invention belongs to the field of microbe biotechnology and relates to the field of biological pesticides. Two trans-aconitic acid producing bacteria, that is, bacillus thuringiensis YBT-1501 and bacillus thuringiensis YBT-1520 are obtained from bacillus thuringiensis through screening; through utilizing the fermentation products of the two strains, the coarse and refine product of the trans-aconitic acid can be obtained; according to bioassay, the trans-aconitic acid has the activity of poisoning and killing meloidogyne incognita, heterodera glycine ichinohe and ditylenchus destructor, and the activity of suppressing the growth of cotton bollworm and housefly. The trans-aconitic acid producing bacteria are novel biological disinsection pesticides and a novel prevention and cure way for the current prevention and cure strategy is provided. The bacillus thuringiensis YBT-1501 which is used for preparing the trans-aconitic acid is preserved in the China Center for Type Culture Collection, with the serial number of CCTCC NO: M2012236.
Owner:HUAZHONG AGRI UNIV

Heterodera avenae Ha-16674 protein, encoding gene and application thereof

The invention relates to heterodera avenae Ha-16674 protein, an encoding gene and application thereof. An amino acid sequence of the heterodera avenae Ha-16674 protein is shown as SEQ ID NO: 1, and a cDNA full-length nucleotide sequence of a gene for encoding the heterodera avenae Ha-16674 protein is shown as SEQ ID NO: 2. Experiments show that the Ha-16674 and Bax can inhibit blade cell death caused by the Bax when being jointly injected into tobacco leaves. Full-length overexpression of the Ha-16674 gene in arabidopsis thaliana can obtain stable genetic T3 generation transgene positive plants, and calprotectin Ha-16674 in heterodera avenae can reduce immune defence reaction of the arabidopsis thaliana to promote pesudomona syringae infection. The heterodera avenae Ha-16674 protein disclosed by the invention provides a new target Ha-16674 for nematode-resistance plant culture and has good theoretical guidance significance on culturing broad-spectrum heterodera-resistance crops.
Owner:INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI

Insecticidal composition containing organophosphorus compound

The invention discloses an insecticidal composition containing an organophosphorus compound. In an insecticidal composition containing an active ingredient A and an active ingredient B, the active ingredient A is selected from lythidathion; the active ingredient B is any one selected from the following compounds: chlorpyrifos, profenofos, triazophos, malathion and phoxim; and the weight ratio of the active ingredient A to the active ingredient B is 1:80-80:1. By using the composition disclosed by the invention, multiple pests can be controlled; obvious synergistic action is obtained; the insecticidal spectrum is expanded; higher activities are obtained for grubs, mole crickets, heterodera, cotton bollworms, pink bollworms, rice planthoppers, Cnaphalocrocis medinalis, striped rice borers, rice water weevils, beet armyworms, aphids, armyworms, budworms and the like on vegetables, rice, cottons, corns, wheat, fruit trees and soybeans; the pesticide usage is reduced; the residual quantity of the pesticide on the crops is reduced; and the environment pollution is reduced; and the insecticidal composition containing the organophosphorus compound, disclosed by the invention, is safe for people and livestock, shows excellent environment compatibility and is not easy for the pests to generate resistance.
Owner:SHAANXI MEIBANG PHARMA GRP CO LTD

Bacillus thuringiensis for controlling heterodera glycines and meloidogyne incognita as well as preparation and applications

ActiveCN108070535AAchieved remarkable resultsBiocideBacteriaMicroorganismAureobasidium sp.
The invention belongs to the technical field of microbial pesticides and particularly relates to bacillus thuringiensis for controlling heterodera glycines and meloidogyne incognita as well as a preparation and applications. The bacillus thuringiensis HAN055 having high toxicity for the heterodera glycines and meloidogyne incognita is obtained through separation. The preparation WP-HAN055 preparedfrom the strain has significant control effects on heterodera glycines and meloidogyne incognita damage. The bacillus thuringiensis HAN055 is collected in CCTCC (China Center for Type Culture Collection) with the collection number of CCTCC NO: M2015608.
Owner:HUAZHONG AGRI UNIV +1

Application of tea saponin in prevention and treatment on soybean sporocyst nematodiasis

ActiveCN104068020AEasy to degradeHas low toxicity and low residueBiocideNematocidesGlycineMedicine
The invention relates to application of tea saponin in prevention and treatment on soybean sporocyst nematodiasis, and belongs to the field of biological prevention and treatment. The tea saponin serves as a main effective component, is high in toxic activity to heterodera glycines, and can be effectively used for preventing and treating the soybean sporocyst nematodiasis.
Owner:SOUTH CHINA AGRI UNIV +1

Preparation method of biocontrol fungus gloeotinia having plant nematode poisoning activity

The invention relates to a culturing method for a fungus having plant nematode poisoning activity and a preparation method of a metabolite of the fungus, belonging to the technical field of microbial pesticides. A fungus strain Snef5 related to the invention is gloeotinia nematicida which is collected in the China General Microbiological Culture Collection Center (Address: Datun Road, Chaoyang District, Beijing, Post Code: 100101) with the collection number CGMCC No.4549 on January 24, 2011. The taxonomic statuses of the fungus strain Snef5 are ascomycota, ascomycetes, helotiales and sclerotiniaceae, and gloeotinia belongs to a fungus. The fungus is prepared by performing liquid fermentation culturing. A metabolite of the fungus has very high toxicity on major pathogen nematodes in plant nematodes such as root-knot nematodes, soybean cyst nematodes and aphelenchoides besseyi, a remarkable nematode killing effect and good application and development prospects.
Owner:SHENYANG AGRI UNIV

Pest-killing and disease-resistant suspended seed coating agent

The invention relates to a pest-killing and disease-resistant suspended seed coating agent. The seed coating agent is prepared by processing active ingredients and non-active ingredients, wherein the active ingredients comprise cyantraniliprole, difenoconazole and a plant growth regulation agent; the non-active ingredients comprise a wetting dispersant, a film forming agent, a microelement fertilizer, a stress-resistant agent, an anti-freezing agent, a preservative, a defoaming agent, a thickening agent, a coloring agent and the like. The suspended seed coating agent has a good pesticide effect, a persistent effect and high work efficiency, is economical and safe and is mainly used for controlling wheat powdery mildew, wheat aphid, cotton fusarium wilt, cotton aphid, a maize take-all disease, thrips, soybean root rot, heterodera glycines ichinohe and other kinds of pest and disease damage.
Owner:海南博士威生物科技有限公司

Streptomyces zaomyceticus Snea182 capable of inducing heterodera glycines resistance of soybean and application

The invention relates to a culture medthod for Streptomyces zaomyceticus Snea182 capable of inducing heterodera glycines resistance of soybeans after soybean seeds are treated with the strain and application of the strain. The actinomycetes strain Snea182 provided by the invention is already preserved in the China General Microbiological Culture Collection Center. After the strain is subjected tofermentation culture with a common liquid, a seed treatment agent for treating soybean seeds can be developed from metabolite of the strain, and the resistance of soybeans of the soybean seeds treatedwith the strain to heterodera glycines can be induced. After the soybean seeds are treated with the Streptomyces zaomyceticus Snea182 metabolite, remarkable resistance of soybeans to a first generation of heterodera glycines in a seedling period can be induced, a remarkable inhibition function on formation of heterodera glycines cytocyst is achieved, the strain is a potential biocontrol bacterium, and a novel idea for preventing and treating heterodera glycines diseases is developed.
Owner:项鹏

Method for aided detection of heterodera glycines resistance and special primers for detection of heterodera glycines resistance

The invention discloses a method for aided detection of heterodera glycines resistance and special primers for detection of heterodera glycines resistance. A primer pair for detecting the resistance of the heterodera glycines to be tested is provided and is composed of a single-stranded DNA molecule shown in sequence 1 in a sequence table and a single-stranded DNA shown in sequence 2 in the sequence table. The experiment provided by the invention shows that the primers designed by the invention can be used for specifically identifying the race 3 physiological race soybean of the heterodera glycines with high sensitivity and high accuracy, the selecting efficiency of the material in the breeding process can be greatly increased and the breeding material can be screened.
Owner:INST OF CROP SCI CHINESE ACAD OF AGRI SCI

Novel chorismate mutase gene from the potato cyst nematode globodera rostochiensis

The nucleotide sequence of a 992 bp region of cDNA and the nucleotide sequence of a 1973 bp (or a 1913 bp) of genomic DNA of the Gr-cm-1 gene were determined for G. rostochiensis. PCR primers and probes specific for G. rostochiensis and G. pallida were generated. PCR assays, including a real-time TaqMan PCR were used to identify G. rostochiensis and G. pallida and to differentiate G. rostochiensis from G. pallida. Transgenic hairy roots expressing Gr-cm-1 dsRNA were generated. There was a 52% reduction in the average number of females per root in the Gr-cm-1 dsRNA transgenic lines when compared with the infected control lines.
Owner:US SEC AGRI

Microbe (SGM-1) and its preparing method

The present invention is one kind of microbe and its preparation process. The microbe is amphibious Monacrosporium janus strain SGM-1 with preservation number of CGMCC No. 1036 used in biological prevention and control of plant Sclerotinia sclerotiorum and Heterodera glycines disease. The strain exhibits relatively strong parasitizing capacity on both Sclerotinia sclerotiorum and Heterodera glycines. Through inoculation with the antagonistic microbe, expanding culture and liquid fermentation, corresponding microbe slurry may be obtained; and the microbe slurry may be mixed with diatomite as carrier to prepare bacteria preparation for biological prevention and control of plant Sclerotinia sclerotiorum and Heterodera glycines disease.
Owner:中国农业科学院生物防治研究所

Bacterium with induced resistance activity and cultural method and application thereof

The invention discloses bacterium with an induced resistance activity and a cultural method and application thereof, wherein the bacterium is enterobacter cloacae and the preservation number of the enterobacter cloacae is CGMCC No. 10801. The bacterium with an induced resistance activity or the metabolite thereof can be used for preparation of a soybean seed treating agent and biological prevention and control of plant parasitic nematodes. The enterobacter cloacae (E.cloacae) Sneb572 or the metabolite thereof has an activity of inducing soybeans, can be used for soybean seed treatment, therefore not only is the growth of soybean plants and roots promoted, but also a great inhibiting effect on formation of heterodera glycines cysts is achieved, and the problem of prevention and control of heterodera glycines is solved.
Owner:SHENYANG AGRI UNIV

Method for screening reference gene in real-time quantitative PCR analysis of heterodera glycines infected wild soybean root tissue

InactiveCN107338322AExploring the mechanism of resistanceMicrobiological testing/measurementReference genesHigh resistance
The invention provides a method for screening a reference gene in the real-time quantitative PCR analysis of a heterodera glycines infected wild soybean root tissue, and relates to the fields of biology and genetics. A high-resistance high-sensitive wild soybean germplasm is taken as a test material, the condition of gene expression of 24 candidate housekeeping genes in a wild soybean root system in different heterodera glycines infection periods is detected, the expression abundance and the expression stability are evaluated so as to screen the reference gene suitable for studying the expression of the gene in the heterodera glycines infected wild soybean root system, and the method is used for exploring the resistance mechanism of the wild soybean to the heterodera.
Owner:JILIN ACAD OF AGRI SCI
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