Preparation method of streptomyces having activity for poisoning plant nematodes and uses thereof
A technology for killing nematodes and plants, which is applied in the field of actinomycetes poisoning and killing plant-parasitic nematodes and the preparation thereof, and can solve the problems of strong sterilization, environmental pollution, and large waste of chemical nematicides.
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Embodiment 1
[0017] Example 1: Cultivation of bacterial strain Snea253
[0018] A single colony of Snea253 was inoculated on the test tube agar medium, and the medium formula was GA medium, that is, KNO 3 1g; K 2 HPO 4 0.5g; MgSO 4 0.5g; NaCl 0.5g; FeSO 4 0.01g; 20g soluble starch; 17g agar; 1000mL water. Cultivate at 28°C for 5 days to obtain the test tube slant strain.
[0019] Then test tube slant strain is inoculated into 250mL triangular flask (50mL of every bottle) liquid medium, and medium formula is (percentage by weight): contain soybean cake powder 15%, soluble starch 10%, glucose 20%, ammonium sulfate 2.5% 、K 2 HPO 4 0.2%, CaCO 3 1%, NaCl 4%, the rest is water, and the pH is 7.2. At 25°C to 28°C, the shaker speed is 150r·min -1 ~200r·min -1 , fermentation 240h. The fermented broth was made into different multiples to carry out the nematicide efficacy test.
Embodiment 2
[0020] Example 2: Nematicidal activity of strain Snea253:
[0021] On the basis of the stock solution of the fermentation broth, it was diluted successively to 1×, 5×, 10×, 15×, 20×, and the nematicidal activity tests were carried out on soybean cyst nematode second instar larvae, root-knot nematode incognita and soybean cyst nematode respectively. Cyst hatching inhibition assay.
[0022] 1. Preparation of preparations for testing
[0023] For the actinomycete strain Snea253 prepared by the aforementioned liquid fermentation culture method, the nematicidal activity test was carried out with different multiples of the fermentation broth.
[0024] 2. Preparation of Nematodes for Experiments
[0025] 2.1 Obtaining soybean cyst nematode eggs and second instar larvae
[0026]Soybean cyst nematode (Heterodera glycines): race 3 of soybean cyst nematode was collected from the experimental field of Northern Nematology Research Institute of Shenyang Agricultural University, and the c...
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