Sampling apparatus for plant and nematode and detection method thereof

Abstract

The invention provides a sampling device used for plant nematode and soil nematode and a detection method thereof, relating to the plant nematode and the soil nematode. The sampling device is provided with a box body, an atomizer and at least one set of separation device; the box body is provided with a movable box cover; the position in the box body close to the bottom is provided with a grid-typed cushion plate; one side of the box body close to the bottom is provided with an drainage valve; the atomizer is arranged at the center of the box body; the separation device is provided with a screen, a screen bracket and nematode receiving utensils; the screen is sheathed on the screen bracket and arranged above the nematode receiving utensils; and the separation device is put on the grid-typed cushion plate. Plant root samples or soil samples are put in the screen; the box cover is opened, the screen bracket is sheathed on the nematode receiving utensil, subsequently, the screen with embedded samples is arranged on the screen bracket; the separation device in the sampling device of plant nematode and the soil nematode is uniformly put in the box body and the box cover is covered; the power of the atomizer is turned on and the atomizer generates water mist; after 12-24 hours, the power is turned off, the box cover is opened and the nematode receiving utensils are sequentially taken out and put under a binocular dissecting microscope and an advanced microscope so as to check the nematode and the types of the nematode.

Description

technical field [0001] The invention relates to plant nematodes and soil nematodes, in particular to a sampling device for plant nematodes and soil nematodes and a detection method thereof. Background technique [0002] Nematode disease is a kind of disease of many plants, which reduces the yield of crops by 30% to 50%, causing huge economic losses. Because most of the nematodes exist in the roots of plants, they are often overlooked and misdiagnosed, which not only causes unnecessary economic losses and disease epidemics, but also abuses pesticides, pollutes crops, and pollutes the environment. Therefore, it is very necessary to isolate nematodes from plant tissues and soil and identify their species for correct diagnosis. [0003] Usually, the nematode detection method is followed by the Baermann funnel method. The basic device is a funnel with a suitable diameter. The funnel is placed on the ring of the iron frame, and it is filled with clean water. Take the fine roots...

AI Technical Summary

Problems solved by technology

Although this method can isolate nematodes, it has the following obvious disadvantages: 1) many of the nematodes obtained by separation are dead; 2) in seasons with high temperature, water often stinks; multiple samples, low work efficiency, multiple funnels occupy laboratory space, and waste water

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