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Preparation method of biocontrol fungus gloeotinia having plant nematode poisoning activity

A technology for biocontrol of fungi and nematodes, applied in the field of fungi for poisoning and killing plant parasitic nematodes and its preparation, capable of solving problems such as polluting the environment, high toxicity and high residues, and destroying soil biota

Inactive Publication Date: 2011-11-23
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the control of plant parasitic nematodes is still dominated by chemical control, but in recent years, the application of chemical nematicides is wasteful, pollutes the environment, has high toxicity and high residue, and damages soil biota. , Zhou Wei et al. 1991. The role of fungi and bacteria in nematode biocontrol and their research progress. Insecticidal Microbes. 3: 55-66), the application of some effective chemical nematocides has been restricted one after another. Research on biological control of nematodes has naturally become a hot issue

Method used

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  • Preparation method of biocontrol fungus gloeotinia having plant nematode poisoning activity
  • Preparation method of biocontrol fungus gloeotinia having plant nematode poisoning activity
  • Preparation method of biocontrol fungus gloeotinia having plant nematode poisoning activity

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Experimental program
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Effect test

Embodiment 1

[0009] Example 1: Cultivation of strain Snef5

[0010] A single colony of Snef5 was inoculated on the agar medium on the slant of the test tube, and the medium formula was PSA medium: 200 g of potatoes, 20 g of sucrose, 17 g of agar, 1000 ml of water, and cultured at 28 ° C for 7 days to obtain the strains of the slant of the test tube.

[0011] Then inoculate the bacteria on the slant of the test tube into a 150mL Erlenmeyer flask (50mL per bottle) liquid medium, the medium formula is containing (NH 4 ) 2 SO 4 3.67g, KCl 0.96g, FeSO 4 0.02g, K 2 HPO 4 0.64g, MgSO 4 ·5H 2 O 1.04g, sucrose 28.3g, distilled water 1000ml, pH 7.2, at 25℃~28℃, shaker speed at 150r min -1 ~200r·min -1 , fermentation 168h. The cultivated fermented liquid is suction-filtered through a Buchner funnel with filter paper to remove the mycelia and obtain nematicidal active substances in the filtrate. The fermented filtrate was made into different multiples to carry out the nematicide efficacy ...

Embodiment 2

[0012] Example 2: Determination of nematicidal activity of bacterial strain Snef5

[0013] The fermentation filtrate was diluted sequentially to 2×, 5×, 10×, 20×, 50× on the basis of the original solution, and the nematicidal activity tests were carried out on soybean cyst nematode J2, root-knot nematode incognita J2 and rice stem-knot nematode respectively. In a sterilized special Belleville dish, add 1 mL each of the fermentation broth diluted 2×, and add 1 mL of sterile water as a control, and then add soybean cyst nematodes and root-knot nematodes to the treatment and control respectively. About 100 nematodes or rice stem apex nematodes were placed in a 25°C incubator, and the nematode mortality was recorded at 24h and 48h respectively, and the corrected mortality was calculated as the nematicidal efficacy. Each treatment was repeated 3 times.

[0014]

[0015]

[0016] test results

[0017] The nematicidal effect of Snef5 fermentation filtrate against root-knot ne...

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Abstract

The invention relates to a culturing method for a fungus having plant nematode poisoning activity and a preparation method of a metabolite of the fungus, belonging to the technical field of microbial pesticides. A fungus strain Snef5 related to the invention is gloeotinia nematicida which is collected in the China General Microbiological Culture Collection Center (Address: Datun Road, Chaoyang District, Beijing, Post Code: 100101) with the collection number CGMCC No.4549 on January 24, 2011. The taxonomic statuses of the fungus strain Snef5 are ascomycota, ascomycetes, helotiales and sclerotiniaceae, and gloeotinia belongs to a fungus. The fungus is prepared by performing liquid fermentation culturing. A metabolite of the fungus has very high toxicity on major pathogen nematodes in plant nematodes such as root-knot nematodes, soybean cyst nematodes and aphelenchoides besseyi, a remarkable nematode killing effect and good application and development prospects.

Description

Technical field: [0001] The invention belongs to the technical field of microbial pesticides, and in particular relates to a fungus for poisoning and killing plant parasitic nematodes and a preparation method thereof. Background technique: [0002] Plant parasitic nematodes occur and cause damage in many countries and regions in the world, and there are various types. On a global scale, the occurrence and harm of plant parasitic nematodes are becoming more and more serious along with the development of agriculture and forestry and the changes of farming and cultivation systems. According to Esser statistics, until 1990, 207 genus and 4832 species of plant parasitic nematodes have been reported to be found in the whole world (Liu Weizhi, Duan Yuxi. 2000. Plant Pathogenic Nematodes. Beijing: China Agricultural Press, p1-2). Plant-parasitic nematodes are estimated to cause an annual loss of 12.3% of the world's major crops, more than $100 billion, and the actual losses far exc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P5/00C12R1/645
Inventor 陈立杰段玉玺赵迪刘彬王媛媛朱晓峰
Owner SHENYANG AGRI UNIV
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