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1163 results about "Arabidopsis sp." patented technology

Plant genome sequences and uses thereof

The present invention is in the field of plant biochemistry and genetics. More specifically the invention relates to nucleic acid sequences from plant cells, in particular, genomic DNA sequences from Arabidopsis thaliana plants. The invention encompasses nucleic acid molecules present in non-coding regions as well as nucleic acid molecules that encode proteins and fragments of proteins. In addition, the invention also encompasses proteins and fragments of proteins so encoded and antibodies capable of binding these proteins or fragments. The invention also relates to methods of using the nucleic acid molecules, proteins and fragments of proteins, and antibodies, for example for genome mapping, gene identification and analysis, plant breeding, preparation of constructs for use in plant gene expression, and transgenic plants.
Owner:CAO YONGWEI +1

Plant artificial chromosome compositions and methods

The present invention provides for the identification and cloning of functional plant centromeres in Arabidopsis. This will permit construction of stably inherited plant artificial chromosomes (PLACs) which can serve as vectors for the construction of transgenic plant and animal cells. In addition, information on the structure and function of these regions will prove valuable in isolating additional centromeric and centromere related genetic elements and polypeptides from other species.
Owner:UNIVERSITY OF CHICAGO

Three cotton ABF/AREB/ABI5/DPBF type transcription factors and coding genes and application thereof

The invention discloses three novel cotton ABF / AREB / ABI5 / DPBF type transcription factors GhABF2, GhABF3 and GhABI5, and coding genes and application thereof. Amino acid sequences of the GhABF2, GhABF3 and GhABI5 are shown as SEQ ID NO: 2, 5 and 8 respectively. Nucleotide sequences of the coding genes GhABF2, GhABF3 and GhABI5 are shown as SEQ ID NO: 1, 4 and 7 respectively. The invention also discloses the structural characteristics on genomic deoxyribonucleic acid (DNA) and a ribonucleic acid (RNA) editing law of reading frame regions of the coding genes GhABF2, GhABF3 and GhABI5. The invention also discloses the cotton expression characteristics and yeast expression characteristics of the three genes and the characteristic that the three genes can improve the drought resistance of arabidopsis thaliana. The genes provide gene resources for culturing a plant variety resistant to abiotic stress, and have great significance for improving the abiotic stress resistance of plants.
Owner:THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI +1

Plants having enhanced yield-related traits and a method for making the same

The present invention relates generally to the field of molecular biology and concerns a method for enhancing various economically important yield-related traits in plants. More specifically, the present invention concerns a method for enhancing yield-related traits in plants by modulating expression in a plant of a nucleic acid encoding an ASPAT (Asparatate AminoTransferase) polypeptide. The present invention also concerns plants having modulated expression of a nucleic acid encoding an ASPAT polypeptide, which plants have enhanced yield-related traits relative to control plants. The invention also provides hitherto unknown ASPAT-encoding nucleic acids and constructs comprising the same, useful in performing the methods of the invention. Furthermore, the present invention relates generally to the field of molecular biology and concerns a method for increasing various plant yield-related traits by increasing expression in a plant of a nucleic acid sequence encoding a MYB91 like transcription factor (MYB91 ) polypeptide. The present invention also concerns plants having increased expression of a nucleic acid sequence encoding an MYB91 polypeptide, which plants have increased yield- related traits relative to control plants. The invention additionally relates to nucleic acid sequences, nucleic acid constructs, vectors and plants containing said nucleic acid sequences. Even furthermore, the present invention relates generally to the field of molecular biology and concerns a method for improving various plant growth characteristics by modulating expression in a plant of a nucleic acid encoding a GASA (Gibberellic Acid-Stimulated Arabidopsis). The present invention also concerns plants having modulated expression of a nucleic acid encoding a GASA, which plants have improved growth characteristics relative to corresponding wild type plants or other control plants. The invention also provides constructs useful in the methods of the invention. Yet furthermore, the present invention relates generally to the field of molecular biology and concerns a method for enhancing various economically important yield-related traits in plants. More specifically, the present invention concerns a method for enhancing yield-related traits in plants by modulating expression in a plant of a nucleic acid encoding an AUX / IAA (auxin / indoleacetic acid) polypeptide. The present invention also concerns plants having modulated expression of a nucleic acid encoding IAA polypeptide, which plants have enhanced yield-related traits relative to control plants. The invention also provides constructs comprising AUX / IAA-encoding nucleic acids, useful in performing the methods of the invention.
Owner:BASF PLANT SCI GMBH

Application of Arabidopsis transcription factor in breeding drought-resistant salt-tolerant rice

The invention relates to application of Arabidopsis transcription factor in breeding drought-resistant salt-tolerant rice. Nucleotide sequences of the Arabidopsis transcription factor MYB44 are shown in SEQ ID NO.1. Encoded protein sequences of the Arabidopsis transcription factor MYB44 are shown in SEQ ID NO.2. Conditions that are met for the nucleotide sequences and the encoded protein sequences include: first, DNA (deoxyribonucleic acid) sequences shown on the 88th site to the 1005th site in the sequence table SEQ ID NO.1, or the sequences highly homologous to the DNA sequences shown on the 88th site to the 1005th site in the SEQ ID NO.1; second, other encodable sequencecs with the same protein as the DNA sequences shown in the sequence table SEQ ID NO.2; third, the sequences the same functional as the DNA sequences shown on the 88th site to the 1005th site in the SEQ ID NO.1, or sub-segments contained in the highly homologous DNA sequences shown on the 88th site to the 1005th site in the SEQ ID NO.1. The Arabidopsis transcription factor is applied to breeding of the drought-resistant salt-tolerant rice. An expression vector of the MYB44 gene can be introduced into plant cells by biotechnology. Transforming hosts, available to use the expression vector containing the MYB44 gene, can be monocotyledons such as rice, corn and wheat. The Arabidopsis transcription factor is also applicable to dicotyledons such as tobacco and soybean. The Arabidopsis transcription factor is used to breed drought-resistant salt-tolerant plant varieties.
Owner:UNIV OF SCI & TECH OF CHINA

Method for increasing contents of tanshinone and salvianolic acid in salvia miltiorrhiza hairy root by using transgene AtMYC2

The invention relates to a method for increasing the contents of tanshinone and salvianolic acid in a salvia miltiorrhiza hairy root by using a transgene AtMYC2, belonging to the technical field of gene engineering. The method comprises the steps of constructing a high-efficiency expression vector of a plant by using an arabidopsis transcription factor AtMYC2, and carrying out genetic transformation on salvia miltiorrhiza leaves to obtain a gene AtMYC2 overexpressed transgenetic salvia miltiorrhiza hairy root; analyzing the expression of AtMYC2 in the transgenetic salvia miltiorrhiza hairy root and related genes in biosynthetic pathways of tanshinone and salvianolic acid through qRT-PCR; measuring the contents of tanshinone and salvianolic acid in the transgenetic salvia miltiorrhiza hairy root by using a high-performance liquid chromatography (HPLC); and measuring the antioxidant activity of tanshinone and salvianolic acid in the transgenetic salvia miltiorrhiza hairy root by using a DPPH free radical scavenging method. The invention provides the method for simultaneously increasing the contents of tanshinone and salvianolic acid in salvia miltiorrhiza hairy root and also provides a novel high-quality raw material for producing tanshinone and salvianolic acid with important clinic demands so as to have the positive promoting significance and application value for relieving the problem that the drug resources of tanshinone and salvianolic acid are short.
Owner:SHANGHAI NORMAL UNIVERSITY

Method for predicting N6-methyladenosine modification site in RNA based on stacking integration

The invention discloses a method for predicting an N6-methyladenosine modification site in RNA based on stacking integration, belonging to the field of systems biology. The method comprises the following steps: extracting RNA sequence features of three species, namely saccharomyces cerevisiae, homo sapiens and arabidopsis thaliana through six feature extraction methods, and conducting feature fusion to obtain an initial feature space of an original data set; performing dimensionality reduction on the initial feature space by using an elastic network, eliminating redundant and noise features, and reserving important features related to model classification so as to obtain an optimal feature set; inputting optimal feature subsets and corresponding category labels into stacking integration for model training, and evaluating the prediction performance of a model in combination with evaluation indexes to obtain a prediction model; and inputting a to-be-predicted RNA sequence in a test set into the prediction model, predicting the m6A site and outputting the m6A site. The prediction accuracy of the model on the test set reaches 92.30% and 87.06% respectively, and the model has good development potential in the aspect of cross-species prediction and is expected to become a useful tool for identifying the m6A site.
Owner:QINGDAO UNIV OF SCI & TECH

Application of corn CIPK42 protein and coding gene of corn CIPK42 protein in regulation and control of salt stress tolerance of plants

The invention relates to the technical field of plant genetic engineering, and particularly discloses an application of a corn CIPK42 protein and a coding gene of the corn CIPK42 protein in regulationand control of salt stress tolerance of plants. The corn ZmCIPK42 gene is found to be able to positively regulate and control the salt tolerance of the plants; and the salt tolerance of the plants can be effectively improved by increasing of the expression quantity of the ZmCIPK42 gene. According to the invention, transgenic corn and arabidopsis thaliana plants with ZmCIPK42 overexpression are constructed; and compared with a non-transgenic wild type, the transgenic corn and arabidopsis thaliana plants are significantly improved in salt tolerance and growth. Discovery of the salt-tolerant function of the ZmCIPK42 gene provides a novel gene target and resource for cultivation of salt-tolerant plant varieties, is of great significance to research of a salt-tolerant molecular mechanism of the plants, and lays a certain theoretical foundation for research of a salt stress response mechanism of the plants and a molecular mechanism of resisting adverse environments.
Owner:新疆农业科学院核技术生物技术研究所(新疆维吾尔自治区生物技术研究中心)

A transcription factor PwNAC2 related to plant stress tolerance, a coding gene thereof and applications of the transcription factor

A transcription factor PwNAC2 related to plant stress tolerance, a coding gene thereof and applications of the transcription factor are disclosed. Protein provided by the invention is a), b), c) or d), wherein the a) is protein the amino acid sequence of which is shown as a sequence 2; the b) is fusion protein obtained by connecting a label to the N end and / or C end of the protein shown as the sequence 2; the c) is protein having same functions and obtained by substitution and / or deletion and / or addition of one or a plurality of amino acid residues to the amino acid sequence shown as the sequence 2; and the d) is protein which has functions same to functions of the amino acid sequence shown as the sequence 2, and which has 75% or 75% or above of homology with the amino acid sequence shownas the sequence 2. The new gene PwNAC2 is found and is introduced into arabidopsis thaliana to obtain a PwNAC2-transgenicarabidopsis thaliana plant. Experiments prove that drought tolerance and salt tolerance of the PwNAC2-transgenicarabidopsis thaliana plant are significantly improved, thus proving that the PwNAC2 or the protein coded by the PwNAC2 has stress tolerance.
Owner:BEIJING FORESTRY UNIVERSITY

WRKY45 gene of paddy rice, preparation method and application

This invention discloses a method for preparing rice gene WRKY45 and its applications. This invention successfully separates gene WRKY45 from rice, determines its nucleotide sequence, and deduces its amino acid sequence. Gene functional analysis shows that rice gene WRKY45 can effectively improve the disease and drought resistance of Arabidopsis thaliana, rice, economic crops, horticultural plants, and pasture grass, thus has wide potential applications.
Owner:XISHUANGBANNA TROPICAL BOTANICAL GARDEN CHINESE ACAD OF SCI

Sesame drought resisting and salt-tolerant gene SiMYB75 as well as encoded protein and application thereof

InactiveCN109337915AIncreased drought and salt toleranceImprove drought and salt toleranceMicrobiological testing/measurementPlant peptidesGenetic engineeringDrought resistance
The invention belongs to the technical field of genetic engineering and provides a sesame drought resisting and salt-tolerant gene SiMYB75 as well as encoded protein and application thereof. The sesame drought resisting and salt-tolerant gene SiMYB75 is a nucleotide sequence shown as SEQ ID NO:1 or a nucleotide sequence with drought resistance and salt tolerance, generated by adding, displacing, inserting or deleting one or more nucleotides. The gene SiMYB75 disclosed by the invention is constructed to an expression vector and is transferred into arabidopsis by a agrobacterium tumefaciens medicated transformation method to obtain transgenic arabidopsis; by overexpression of the drought resisting and salt-tolerant gene SiMYB75 in the arabidopsis, the gene is found to remarkably improve thedrought resistance and the salt tolerance of plants. Therefore, the sesame drought resisting and salt-tolerant gene SiMYB75 has a good application prospect in improving the properties of the plants, such as the drought resistance and the salt tolerance.
Owner:INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI

Thellungiella salsuginea transcription factor EsMYB41 for controlling plant anthocyanin synthesis and encoding gene and application thereof

The invention belongs to the technical field of agricultural biology, and particularly relates to a thellungiella salsuginea transcription factor EsMYB41 for controlling plant anthocyanin synthesis and an encoding gene and application thereof. The amino acid sequence of the thellungiella salsuginea transcription factor EsMYB41 for controlling plant anthocyanin synthesis is shown in SEQ ID No.1. Byconstructing an EsMyb41 containing over expression vector, tobacco and arabidopsis are transferred, and EsMYB41 transgenic tobacco and arabidopsis are obtained; results find that the transgenic planthas the almost whole-plant claret-colored phenotype; the anthocyanin content of different tissues of transgenic plants and the expression of the anthocyanin synthesis structure gene are both remarkably higher than the wild type, and meanwhile under the salt stress, the anti-oxidase capability of the transgenic plant is improved remarkably.
Owner:SHANDONG NORMAL UNIV

Compositions And Methods Of A Phosphatidic Acid Binding Protein

TGD2 proteins of Arabidopsis are proposed to be a substrate binding component of a lipid transfer complex in the inner chloroplast envelope membrane. Loss of function of this protein or other components of this complex may disrupt the endoplasmic reticulum (ER)-pathway of thylakoid lipid biosynthesis. In one embodiment, the present invention contemplates a minimal binding domain capable of specifically binding phosphatidic acid. Alternatively, the minimal binding domain may further comprise accessory binding domains that, in combination, create a complete TGD2 phosphatidic acid binding domain. Consequently, phosphatidic acid may be quantitatively detected from samples as described in the methods herein.
Owner:BOARD OF TRUSTEES OPERATING MICHIGAN STATE UNIV
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