83 results about "Metabolism regulation" patented technology

The invention discloses an application of an activator protein prepared from Alternaria tenuissima by microbial fermentation to a pesticide preparation. The activator protein from Alternaria tenuissima which is prepared from Alternaria tenuissima by fermentation and special extraction processes is a single and stable protein with biological activities. The protein can be prepared with glucosamine oligosaccharides and related protein protectants into wettable powder, the protein can be combined with a receptor protein on a plant cell membrane, in order to induce increases of a series of related enzymatic activity and gene expression quantity in the plant, stimulate a series of metabolism regulation in the plant, promote growth of roots, stems and leaves of the plant, increase chlorophyll content, and increase crop yield. The wettable powder prepared by the protein from Alternaria tenuissima completely accords with each national index, and has the effects for preventing and controlling plant virus diseases, greatly improving physiological functions of plants, enhancing disease resistance of plants, and increasing crop output to a certain degree.

The invention belongs to the field of environmental protection technology and relates to an enhanced sewage biological nitrogen and phosphorus removal method bases on polyhydroxyalkanoate metabolism regulation. The method comprises the following steps: introducing grain organic matters obtained after primary sedimentation and residual sludge discharged from a secondary sedimentation tank into a sludge tihckener for condensation; conducting anaerobic fermentation on the condensed organic refuses in a production reactor of a polyhydroxyalkanoate precursor to synthesize polyhydroxyalkanoate precursor; removing nitrogen and phosphor, and pumping the liquid into a pool for the polyhydroxyalkanoate precursor; pumping the polyhydroxyalkanoate precursor into a production reactor of polyhydroxyalkanoate to generate polyhydroxyalkanoate; and pumping the sewage into a routine sewage biological nitrogen and phosphorus removal reactor to carry out a sewage biological nitrogen and phosphorus removal process. The method provided by the invention can increase the removal rate of nitrogen and phosphor to 80% and 95%, and the treated outlet water reaches the sewage discharge standard Grade A. The method has advantages of convenient technology control, a small amount of generated greenhouse gas and sludge, and low cost of a reconstruction technology.

The invention relates to high-fiber corn flake healthy food and a production method thereof, belonging to the food processing technology. The method comprises the following steps of: screening corn seeds to remove inedible impurities; rinsing, drying and crushing to obtain pure corn meal; adding a proper amount of high-quality corn dietary fiber, flavor endowing agent and the like, and adjusting the components; and preparing the corn flake healthy food with abnormal metabolism regulation functions such as shaping, resisting constipation and the like by the technology integrating separation and recombination, quick cropping and forming. The product is crispy, has good flavor, is suitable for all consumer groups, and can be taken as leisure food as well as snack or staple food. In the production process, the raw materials are completely used, the production of pollution, waste slag, waste gas and harmful substances is avoided, and the food is safe to eat because no chemical reagent is used.

The invention discloses a promoter for regulating and controlling expression of genes in non-secretory type glandular hairs. The nucleotide sequence of the promoter is shown in SEQ ID No:1. The invention further provides a preparation method of the promoter, a carrier containing the promoter and an expression case. The promoter can regulate and control the expression of target genes in time and space, therefore, morphogenesis and development of the non-secretory type glandular hairs and metabolism regulation of secondary metabolites in the non-secretory type glandular hairs can be intensively studied, and application in genetic engineering breeding for producing the metabolites can be achieved. Therefore, the promoter for regulating and controlling the expression of the genes in the non-secretory type glandular hairs has important significance on genetic engineering breeding for expressing and producing the metabolites by means of plant glandular hair tissue.

A functional compound natto health care product is compounded from natto, blueberry, slash pine needle, kidney bean, kudzuvine root and sorbitol. The compatibility proportions of the raw materials are as follows: 20 to 80 % of natto, 5 to 15 % of blueberry, 2 to 5 % of slash pine needle, 20 to 25 % of kidney bean, 15 to 20 % of kudzuvine root and 2 to 5 % of sorbitol, and the proportion of medicine and auxiliary material is (20 to 80 %) : (80 to 20 %). The health care product can be in the form of capsule, troche, chewable tablet, granula, oral liquid or soft capsule. The product has the health care effects of aging resistance, blood fat reduction, organism metabolism regulation, immunization improvement and the like, and has active social and economic significance on disease prevention, body-building and the realization of industrialization popularization.

In general, this invention relates to nucleic acid and amino acid sequences involved in fat metabolism regulation and the use of these sequences as targets for the diagnosis, treatment, and prevention of obesity and obesity-related diseases. In addition, the invention relates to screening methods for identifying modulators of body fat metabolism and the development of treatments for obesity and obesity-related diseases.

The present invention discloses applications of a grease metabolism related protein GmNF307 in plant grease metabolism regulation. The protein is the following protein a) or b) or c): a) the protein has the amino acid sequence represented by SEQ ID No.2; b) the fusion protein is obtained by linking a tag to the N terminal or / and C terminal of the protection represented by SEQ ID No.2; and c) the protein is obtained by substituting and / or deleting and / or adding one or a plurality of amino acid residues on the amino acid sequence represented by SEQ ID No.2 and is associated with the grease metabolism regulation. According to the present invention, the experiment results show that the contents of the total greases in plant tissues and / or organs and the contents of partial fatty acids in plant tissues and / or organs can be increased with the overexpression of the encoding gene GmNF307 of the grease metabolism related protein GmNF307.

The invention discloses an anti-stress regulator used for promoting growth and improving metabolism of pigs. The regulator is prepared from the following ingredients by weight: retinyl acetate, vitamin D3, d1-alpha-tocopheryl acetate, menadione, thiamine hydrochloride, riboflavin, pyridoxine hydrochloride, cyanocobalamin, L-ascorbic acid, d-biotin, nicotinamide, pantothenic acid, folic acid, methionine, lysine, xylooligosaccharide, citric acid, sodium glutamate and glucose as balance. The invention also relates to a preparation method of the anti-stress regulator used for promoting growth and improving metabolism of pigs. The regulator prepared by the method can stimulate the self-metabolism regulation, can improve the feed utilization rate, can improve the food consumption of the growing-finishing pigs and the reproductive performance of the sows, can improve the anti-stress ability, can reduce the death rate and the diarrhea occurrence rate of piglets, has a remarkable growth promotion effect, can improve the growth speed of the weaned pigs, the growing-finishing pigs and the suckling pigs, and has remarkable economic benefit.

The present invention discloses one kind of hydroxy alkanoic acid polymer, that is, 3-hydroxy butyric acid-3-hydroxy caprylic acid polymer (PHBHO). The 3-hydroxy butyric acid-3-hydroxy caprylic acid polymer (PHBHO) is prepared with Sinorhizobium fredii as production strain, carbohydrate as carbon source and ammonium salt as nitrogen source, and through high density fermentation and metabolism regulation with lauric acid in optimal culture medium and under optimal culture condition. Corresponding fermentation management method, separation and purification method and quality detection method are established. The present invention can obtain PHBHO product up to 14.4-22.1 g / L, of molecular weight of 112000-185000 Da, and endotoxin content 4-6 EU / g, and the novel nanometer level material may be used in biomedicine and medical tissue engineering.

The invention discloses oil and fat metabolism regulation related soybean transcription factor GmMYB172 and a coding gene and application thereof. The protein is named as GmMYB172, is derived from papilionaceae glycine soybean (Glycine max (L.) Merrill), is a protein as follows (a) or (b): (a) protein is composed of an amino acid sequence shown as the sequence 2 in the sequence table; and (b) protein is a protein obtained by substitution and / or deletion and / or addition of one or a plurality of amino acid residues of the amino acid sequence shown as the sequence 2 and is in relevant to plant tissue total oil and fat content and derived from the sequence 2. Experiments show that, the transcription factor GmMYB172 and the coding gene can regulate the oil and fat content in plant seeds, and after over expression, can improve the oil and fat content in the plant seeds. The coding gene has important theoretical and practical significances in the aspects of increasing and improving crop oil and fat ingredients, especially improving oil and fat ingredients in soybean and other oil plant seeds, and cultivating high oil and fat containing varieties.

The invention discloses a method for extracting mitochondria and mitochondrial protein from cotton. The method has minimum mechanical damage to mitochondria, nucleus components and plasmid components except mitochondria as well as phenolic and polysaccharide substances can be removed effectively, the contamination rate is reduced, the purity and the quality of mitochondrial protein are improved, experimental technique requirements of cotton genome research, mitochondrial proteome research, metabolism regulation network research and the like are met, and related research work fields are broadened.

The invention discloses oil and fat metabolism regulation related soybean transcription factor GmZF351 and a coding gene and application thereof. The protein is named as GmZF351, is derived from papilionaceae glycine soybean (Glycine max (L.) Merrill), is a protein as follows (a) or (b): (a) protein is composed of an amino acid sequence shown as the sequence 2 in the sequence table; and (b) protein is a protein obtained by substitution and / or deletion and / or addition of one or a plurality of amino acid residues of the amino acid sequence shown as the sequence 2 and is in relevant to plant tissue total oil and fat content and derived from the sequence 2. Experiments show that, the transcription factor GmZF351 and the coding gene can regulate the oil and fat content in plant seeds, and after over expression, can improve the oil and fat content in the plant seeds. The coding gene has important theoretical and practical significances in the aspects of increasing and improving crop oil and fat ingredients, especially improving oil and fat ingredients in soybean and other oil plant seeds, and cultivating high oil and fat containing varieties.

Isolated nucleic acids and proteins associated with lipid and sugar metabolism regulation are provided. In particular, lipid metabolism proteins (LMP) and encoding nucleic acids originating from Arabidopsis thaliana, Brassica napus, Glycine max, Oryza sativa, and Triticum aestivum are provided. The nucleic acids and proteins are used in methods of producing transgenic plants and modulating levels of seed storage compounds. Preferably, the seed storage compounds are lipids, fatty acids, starches, or seed storage proteins. The nucleic acids and proteins also are used in methods of modulating the seed size, seed number, seed weight, root length, and leaf size of plants.

The invention discloses a hybrid nano-formulation for promoting normalization of tumor blood vessels based on tumor extensive metabolism regulation and application thereof. A nano self-assembly technology based on electrostatic interaction and hydrogen bonding is used to obtain the hybrid nano-formulation. Heparin polysaccharide macromolecules that having VEGF inhibitory activity and derivatives and active biological macromolecules thereof, or active substance targeting vascular endothelial cells are assembled with non-viral gene carriers and metabolic regulation related gene drugs to construct stable nano-drugs targeting tumor cells and tumor vascular endothelial cells, separately. After the hybrid nano-formulation formed only by physically mixing the two ternary assembled nano-drugs areadministered in the manner of beat, significant metabolic regulation effects of tumor cells and tumor vascular endothelial cells, promoting normalization of tumor blood vessels, and the multiple therapeutic effects of tumor proliferation inhibition can be obtained. The three effects are synergistic and complement each other to enhance the effectiveness.

The present invention discloses the production process of hydroxy alkanoic acid polymer. The hydroxy alkanoic acid polymer is prepared with Sinorhizobium fredii as production strain, carbohydrate as carbon source and ammonium salt as nitrogen source, and through high density fermentation and metabolism regulation with caprate in optimal culture medium and under optimal culture condition to obtain 3-hydroxy butyric acid-3-hydroxy caprylic acid polymer. Corresponding fermentation management method, separation and purification method and quality detection method are established. The present invention can obtain 3-hydroxy butyric acid-3-hydroxy caprylic acid polymer product up to 10.7-16.4 g / L, of molecular weight of 127000-137000 Da, and endotoxin content 4-6 EU / g, and the novel nanometer level material may be used in biomedicine and medical tissue engineering.

The invention relates to a technique for preparing Aspergillus fermented black beans by a metabolism regulation process, which comprises the following steps: immersing, boiling, cooling, yeast preparation, fermenting and drying. In the yeast preparation process, a black bean hydrolysate is added to enhance the yeast enzyme activity, shorten the yeast preparation period and provide nutrient substances for secondary fermentation; and in the fermentation process, saline water is added to inhibit infectious microbes in the fermentation process. The invention also relates to fermented black beans produced by the technique. The production technique greatly shortens the production cycle of the fermented black beans, and enhances the nutritive value of the fermented black beans.

The invention belongs to the field of medical biotechnology, and particularly relates to a genetically modified immune cell with a malignant solid tumor treating function and application thereof. The immune cell is a specific immune cell which is subjected to gene modification so as to over-express lipid metabolism-related genes. The tumor growth inhibiting capacity of the immune cell is realized by reducing accumulation of lipid droplets of the immune cell itself in a tumor microenvironment, reducing expression of tumor promotion genes / protein, and improving the phagocytotic ability, antigen presentation ability and tumor killing ability of the immune cell. The immune cell is an in-vitro NKT cell, a DC cell, a macrophage, a monocyte, a granulocyte or a T cell. Overexpression of related metabolism regulation genes enables the anti-tumor capacity of the immune cell to be improved remarkably. Compared with a chimeric antigen receptor T cell immunotherapy (CAR-T) which receives much attention currently, toxic and side effects are small, cytokine storm can not be caused, and clinical requirements can be met.

The animal cell feeding culture process includes the following steps: designing different feeding processes to realize different pseudostable culture environment, determine optimized target and establish metabolism dynamics model; performing the optimized culture process based on the metabolism dynamics model; sampling and detecting ammonia concentration regularly, estimating the concentration of glutamine as the control quantity based on the metabolism dynamics model and feeding back to correct feeding rate to avoid system divergency. The process of the present invention can perform research on metabolism regulations of cell strain fast and accurately and realize cell culture process with high culture medium efficiency, high cell density and product concentration, high stability and controllability. The present invention makes it possible to establish large scale optimized animal cell feeding culture process and is significant in producing bioproduct.

The present invention belongs to the technical field of nutritious foods, and especially relates to a rectal cancer nutritious food and a preparation method thereof. The rectal cancer nutritious foodcontains nutrient elements including proteins, fat, carbohydrate, vitamins, minerals, ginkgo flavonoids and phytosterol. The nutritious food provided by the invention can provide sufficient and comprehensive nutrients for the organism, more importantly, the food enhances the barrier function of intestinal mucosa, better accords with the metabolic physiological state, facilitates the protein synthesis and metabolism regulation of the organism, promotes the recovery of intestinal function and form, prevents bacteria and toxin translocation, obviously reduces the occurrence of intestinal source infection, and is an effective way to improve the nutritional status of chemotherapy patients. The food has a certain improvement effect on the nutritional status of chemotherapy patients with middle or advanced rectal cancer, can reduce adverse reaction of patients, and can effectively improve the immunity of patients.

The invention discloses application of a soybean transcription factor GmAREB3 in plant fat metabolism regulation. The invention provides application of optional one of 1) protein GmAREB3, 2) DNA molecules for encoding the protein GmAREB3 and 3) a recombinant vector, an expression kit, a transgenic cell line or a recombinant bacterium containing the DNA molecules for encoding the protein GmAREB3 in the regulation of plant tissue total fat content and / or the regulation of the content of fatty acid in plant tissue, and the amino acid sequence of the protein GmAREB3 is as shown in the sequence 2 in a sequence table. Experiments show that the transcription factor GmAREB3 and the coding gene thereof can regulate the fat content in plant seeds and can increase the fat content in the plant seeds after overexpression; the gene can increase and improve the fat components of crops especially oil plant seeds such as soybean seeds and has important theoretical and practical significance to the culture of high-fat varieties.

The invention discloses a method for simultaneously detecting the contents of three alkaloids in a lycoris plant, which is capable of quickly extracting and qualitatively and quantitatively analyzingalkaloid matters of galanthamine, lycoramine and lycorine in the lycoris plant. According to the method disclosed by the invention, gradient eluting is carried out by taking an LC (Liquid Chromatography)-MS (Mass Spectrometry) technology as the basis, analysis is carried out in an MRM (Multiple Reaction Monitoring) mode under positive ion scanning by connecting LC with tandem MS, the three alkaloid matters in the lycoris plant can be quickly extracted and accurately, qualitatively and quantitatively analyzed, the analysis time can be shortened to a large extent, the analysis accuracy is increased, a detection result can be obtained within a program time which is not more than 6.0 minutes, the retention time of the three alkaloids is within 3 minutes, the adding standard recovery rate is 97.2 to 100.2 percent, the RSD (Relative Standard Deviation) is 0.8 to 4.3 percent, and the analysis speed and the analysis accuracy are greatly increased; an effective and accurate analysis method is provided for researching an accumulation law and constructive metabolism regulation of the three alkaloids in the lycoris plant.

The invention relates to a resistance inducer for preventing and controlling tobacco bacterial wilt and an application thereof. The resistance inducer for preventing and controlling tobacco bacterialwilt mainly comprises citric acid and a synergist, wherein the synergist is composed of the following raw materials in parts by weight: 1-3 parts of chitosan, 2-4 parts of gibberellin, 10-30 parts ofcalcium nitrate and 1-3 parts of monopotassium phosphate. The resistance inducer for preventing and controlling tobacco bacterial wilt is capable of stimulating a series of metabolism regulation systems in plants to generate resistance substances, inducing resistance of tobacco plants to bacterial wilt or restraining the growth of germs, achieving the purpose of safely and efficiently preventing and controlling tobacco bacterial wilt and realizing 'primary green prevention and control, prevention and control through chemical decrement'.

The invention relates to a strategy for growth and metabolism regulation of microalgae cultured by urban sewage. The strategy mainly includes: (1) in an early culture stage, a plant growth regulator and COD (chemical oxygen demand) degrading bacteria are added into microalgae-containing urban sewage to promote chlorophyll synthesis and degradation of organics in sewage and improve microalgae cellphotosynthetic efficiency and carbon sequestration efficiency to increase biomass yield; (2) in a later culture stage, the nitrogen content of sewage meets a nitrogen deficiency condition, and accordingly a glycolytic pathway is regulated so as to promote carbohydrate metabolism to enable more carbon for fatty acid synthesis, and grease accumulation promotion is realized. Adopting the urban sewagefor culturing microalgae meets the requirement of a two-stage culture process in a same system, both microalgae high-density culture and grease accumulation can be achieved, simplicity and convenience in operation are achieved, and large-scale production is facilitated; the strategy is significant to guidance of large-scale microalgae culture and provides theoretical and technical support for process optimization of an industrial chain for preparing biodiesel from microalgae cultured by urban sewage.

The invention belongs to the field of functional genomics and particularly relates to a method for analyzing a metabolism regulation mechanism of carotenoid in maize grains based on dynamic correlation between co-expression patterns of gene pairs. The method comprises the following steps: downloading 13 key genes in a carotenoid metabolism pathway from a maize genome database maizeGDB; collecting grain transcripts obtained after pollinating maize inbred lines for 15 days, and sequencing to obtain gene expression level data, and extracting expression level data of the 13 key genes; building a dynamic correlation analysis LA model; and analyzing and identifying a regulation relation between the key genes in the pathway utilizing a dynamic correlation analysis. The method is capable of excavating the regulation relation between the 13 key genes in the carotenoid metabolism pathway based on dynamic correlation analysis, analyzing the regulation mechanism of synthesis and accumulation of carotenoid in grains and providing optimal gene combination for molecular-marker assistant breeding, and has important significance for increasing carotenoid in maize and improving maize quality.

A regulation and control technology used for lowering the accumulation of leek azotate aims at the accumulation problem of leek azotate caused by excessively applying nitrogen fertilizer for pursuing high output and the negative solution problem for lowering fertilizer of simply and blindly limiting the application rates of nitrogen fertilizer. The new technology takes the promotion of the reduction and the assimilation of azotate as well the conversion and synthesis into amino acid and protein as an entry point, first creates the positive measures for lowering the accumulation of leek azotate and adopts salicylic acid ( novel metabolism regulation and control hormone) for leaf surface spraying and obtaining the obvious effect of lowering the accumulation of leek azotate, the salicylic acid can obviously lower the accumulation of leek azotate in the face of high nitrogen level, meanwhile, the effects of increasing output and improving the quality can be obtained and the efficiency and the utilization rate of the nitrogen fertilizer can be improved. The appropriate concentration of the leaf surface spraying salicylic acid is 3mmol.L<-1> and the appropriate period for leaf surface spraying is 6 to 9 days before harvesting leek.

The invention discloses application of a cotton transcription factor GaMAN1 to plant grease metabolism regulation and control. The transcription factor GaMAN1 is the protein shown as a) or b) or c) ord): (a) protein with the amino acid sequence shown as the sequence 2; b) fusion protein obtained by connecting tags onto the end N and / or the end C of the protein shown as the sequence 2; c) proteinwith the same function obtained by performing substitution and / or deletion and / or addition of one or several amino acid residues on the amino acid sequence shown by as sequence 2; d) protein with 75 percent or 75 percent or above of homology with the amino acid sequence shown as the sequence 2 and with the same function. Experiments prove that the cotton transcription factor GaMAN1 can improve thecrop grease content and the fatty acid content. The gene has the important theoretical and practical significance on crop grease ingredient increase and improvement, particularly on cotton seed grease ingredient improvement and high-grease variety culture.

The invention relates to a therapeutic drug target for preventing and treating skeletal muscle atrophy and an application of the therapeutic drug target, and provides the application of a BCKDC (branched-chain alpha-ketoacid dehydrogenase complex) or BCKDK (branched-chain alpha-ketoacid dehydrogenase kinase) as the therapeutic drug target to prevention or treatment of skeletal muscle atrophy and an application of the BCKDC or BCKDK to preparation of drugs for preventing or treating skeletal muscle atrophy and particularly provides a method for screening skeletal muscle atrophy resistant drugsin vivo and in vitro. According to the method, the BCKDC or a gene of the BCKDC is taken as a drug action object or BCKDK or a gene of BCKDK is taken as the drug action object, and an inhibitor of theBCKDC or an activator of BCKDK is selected as a primarily screened drug candidate for preventing or treating skeletal muscle atrophy. The key restrictive metabolic enzyme regulation mechanism of thetherapeutic drug target is analyzed from the perspective of tumor cachexia metabolism regulation, the drug intervention target is determined, the therapeutic drug target has potential clinical application value in treatment of skeletal muscle atrophy, and a new platform is provided for screening of the drugs for treating skeletal muscle atrophy.

The invention belongs to the field of a biological food, and specifically, discloses hawthorn fruit fermentation technology with contents of citric acid and tannin reduced. The technology comprises the following steps: (1) selecting, stoning, and cleaning hawthorn fruits; (2) taking 1 part by weight of hawthorn fruits with 1-2 parts of water added for pulping to prepare hawthorn juice, and adding oligomeric glutamic acid chelated magnesium to prepare a fermentation medium; (3) sterilizing the fermentation medium at 121 DEG C for 30 min; (4) after sterilization, performing inoculation with Yeast-Y86, and performing fermentation at 28 DEG C; and (5) when the fermentation is over, performing membrane filtration sterilization to obtain mellow hawthorn juice with a low citric acid content. Yeast-Y86 which is selected in the laboratory and is excellent in degradation of organic acid and tannin is used for fermentation to prepare the hawthorn fruit juice, and the citric acid and tannin are decreased through metabolism regulation during fermentation. The fermented hawthorn fruit juice is low in contents of citric acid and tannin and high in general flavones content, and is mellow and thick.