83 results about "Metabolism regulation" patented technology

Isolated nucleic acids and proteins associated with lipid and sugar metabolism regulation are provided. In particular, lipid metabolism proteins (LMP) and encoding nucleic acids originating from Arabidopsis thaliana, Brassica napus, Glycine max, Oryza sativa, and Triticum aestivum are provided. The nucleic acids and proteins are used in methods of producing transgenic plants and modulating levels of seed storage compounds. Preferably, the seed storage compounds are lipids, fatty acids, starches, or seed storage proteins. The nucleic acids and proteins also are used in methods of modulating the seed size, seed number, seed weight, root length, and leaf size of plants.

The invention relates to a novel method for producing pullulan by using cell metabolism regulation technology to improve cell permeability. 0.02% to 2.14% of a polyether substance and 0.01% to 1.35% of a surfactant substance are added into a fermentation medium, thereby effectively increasing the yield of pullulan, improving the conversion efficiency of a substrate, shortening a fermentation cycle and reducing formation of melanin.

Isolated nucleic acids and proteins associated with lipid and sugar metabolism regulation are provided. In particular, lipid metabolism proteins (LMP) and encoding nucleic acids originating from Arabidopsis thaliana, Brassica napus, Glycine max, Oryza sativa, and Triticum aestivum are provided. The nucleic acids and proteins are used in methods of producing transgenic plants and modulating levels of seed storage compounds. Preferably, the seed storage compounds are lipids, fatty acids, starches, or seed storage proteins. The nucleic acids and proteins also are used in methods of modulating the seed size, seed number, seed weight, root length, and leaf size of plants.

The invention discloses a hybrid nano-formulation for promoting normalization of tumor blood vessels based on tumor extensive metabolism regulation and application thereof. A nano self-assembly technology based on electrostatic interaction and hydrogen bonding is used to obtain the hybrid nano-formulation. Heparin polysaccharide macromolecules that having VEGF inhibitory activity and derivatives and active biological macromolecules thereof, or active substance targeting vascular endothelial cells are assembled with non-viral gene carriers and metabolic regulation related gene drugs to construct stable nano-drugs targeting tumor cells and tumor vascular endothelial cells, separately. After the hybrid nano-formulation formed only by physically mixing the two ternary assembled nano-drugs areadministered in the manner of beat, significant metabolic regulation effects of tumor cells and tumor vascular endothelial cells, promoting normalization of tumor blood vessels, and the multiple therapeutic effects of tumor proliferation inhibition can be obtained. The three effects are synergistic and complement each other to enhance the effectiveness.

The invention discloses a method for simultaneously detecting the contents of three alkaloids in a lycoris plant, which is capable of quickly extracting and qualitatively and quantitatively analyzingalkaloid matters of galanthamine, lycoramine and lycorine in the lycoris plant. According to the method disclosed by the invention, gradient eluting is carried out by taking an LC (Liquid Chromatography)-MS (Mass Spectrometry) technology as the basis, analysis is carried out in an MRM (Multiple Reaction Monitoring) mode under positive ion scanning by connecting LC with tandem MS, the three alkaloid matters in the lycoris plant can be quickly extracted and accurately, qualitatively and quantitatively analyzed, the analysis time can be shortened to a large extent, the analysis accuracy is increased, a detection result can be obtained within a program time which is not more than 6.0 minutes, the retention time of the three alkaloids is within 3 minutes, the adding standard recovery rate is 97.2 to 100.2 percent, the RSD (Relative Standard Deviation) is 0.8 to 4.3 percent, and the analysis speed and the analysis accuracy are greatly increased; an effective and accurate analysis method is provided for researching an accumulation law and constructive metabolism regulation of the three alkaloids in the lycoris plant.

The invention discloses application of a cotton transcription factor GaMAN1 to plant grease metabolism regulation and control. The transcription factor GaMAN1 is the protein shown as a) or b) or c) ord): (a) protein with the amino acid sequence shown as the sequence 2; b) fusion protein obtained by connecting tags onto the end N and / or the end C of the protein shown as the sequence 2; c) proteinwith the same function obtained by performing substitution and / or deletion and / or addition of one or several amino acid residues on the amino acid sequence shown by as sequence 2; d) protein with 75 percent or 75 percent or above of homology with the amino acid sequence shown as the sequence 2 and with the same function. Experiments prove that the cotton transcription factor GaMAN1 can improve thecrop grease content and the fatty acid content. The gene has the important theoretical and practical significance on crop grease ingredient increase and improvement, particularly on cotton seed grease ingredient improvement and high-grease variety culture.