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Strategy for growth and metabolism regulation of microalgae cultured by urban sewage

A technology for urban sewage and metabolic regulation, applied in the direction of microbe-based methods, microbes, biofuels, etc., can solve problems such as high cost and difficult industrial application

Inactive Publication Date: 2018-03-23
HARBIN INST OF TECH SHENZHEN GRADUATE SCHOOL
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Problems solved by technology

[0007] In view of the problems existing in the prior art, the main purpose of the present invention is to provide a strategy for regulating the growth and metabolism of microalgae cultured in urban sewage, which is used to overcome the problems of high cost and difficulty in industrial application of existing microalgae oil extraction methods

Method used

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Embodiment 1

[0035] Chlorella at initial concentration D 680 =0.1 was inoculated into 10L of urban sewage (the concentrations of COD, TN, and TP were 150, 25, and 5.5 mg / L, respectively), and 2,4-dichlorophenoxyacetic acid was added in the early stage of culture (the 0th day of microalgae culture in urban sewage) (2,4-D) The initial concentration is set to 1.5mg / L, and the initial concentration of photosynthetic bacteria is set to D 600 is 0.01. Promote the synthesis of chlorophyll and the degradation of organic matter in sewage, so as to regulate the light reaction and Calvin cycle, improve the photosynthesis efficiency and carbon fixation efficiency of microalgae cells, and achieve the purpose of increasing biomass production; Day) The nitrogen content in sewage meets the condition of nitrogen deficiency (nitrogen concentration is 4.5mg / L), thereby regulating the glycolysis pathway, promoting carbohydrate metabolism, making more carbon flow to fatty acid synthesis, and then achieving th...

Embodiment 2

[0037] Scenedesmus at initial concentration D 680 =0.1 was inoculated into 10L urban sewage (the concentrations of COD, TN and TP were 145, 35 and 6mg / L respectively), and the initial concentration of adenine (6-BA) was added in the early stage of culture (the 0th day of urban sewage culture microalgae). Set to 1mg / L, add the initial concentration of Bacillus subtilis to set D 600 is 0.02. Promote the synthesis of chlorophyll and the degradation of organic matter in sewage, so as to regulate the light reaction and Calvin cycle, improve the photosynthesis efficiency and carbon fixation efficiency of microalgae cells, and achieve the purpose of increasing biomass production; day) The nitrogen content in sewage meets the condition of nitrogen deficiency (nitrogen concentration is 5), thereby regulating the glycolysis pathway, promoting carbohydrate metabolism, allowing more carbon to flow to fatty acid synthesis, and then achieving the purpose of promoting oil accumulation. After...

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Abstract

The invention relates to a strategy for growth and metabolism regulation of microalgae cultured by urban sewage. The strategy mainly includes: (1) in an early culture stage, a plant growth regulator and COD (chemical oxygen demand) degrading bacteria are added into microalgae-containing urban sewage to promote chlorophyll synthesis and degradation of organics in sewage and improve microalgae cellphotosynthetic efficiency and carbon sequestration efficiency to increase biomass yield; (2) in a later culture stage, the nitrogen content of sewage meets a nitrogen deficiency condition, and accordingly a glycolytic pathway is regulated so as to promote carbohydrate metabolism to enable more carbon for fatty acid synthesis, and grease accumulation promotion is realized. Adopting the urban sewagefor culturing microalgae meets the requirement of a two-stage culture process in a same system, both microalgae high-density culture and grease accumulation can be achieved, simplicity and convenience in operation are achieved, and large-scale production is facilitated; the strategy is significant to guidance of large-scale microalgae culture and provides theoretical and technical support for process optimization of an industrial chain for preparing biodiesel from microalgae cultured by urban sewage.

Description

technical field [0001] The invention belongs to the field of microalgae biodiesel, in particular to a strategy for regulating the growth and metabolism of microalgae cultivated in urban sewage. Background technique [0002] The optimization and regulation of the microalgae culture process is an effective means to improve the system's lipid production performance and oil quality. For a long time, the optimization of microalgae cultivation process has mainly focused on promoting the synthesis and accumulation of microalgae lipids through stress conditions; or promoting the growth of microalgae through external stimuli. Due to the contradiction between the growth of microalgae and the accumulation of oil, when the growth rate of microalgae is faster and the biomass is higher, it is often unfavorable for its oil accumulation. How to achieve rapid oil accumulation without affecting the maximum biomass production of microalgae is the key to improving the final oil yield. The mai...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12P7/64C12R1/89
CPCC12N1/12C12P7/6463Y02E50/10
Inventor 金文标涂仁杰韩松芳
Owner HARBIN INST OF TECH SHENZHEN GRADUATE SCHOOL
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