Production method for pullulan based on cell metabolism regulation strategies

A pullulan polysaccharide and cell metabolism technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of complex separation and purification process, difficult to remove, low strain fermentation yield, etc., and achieve the cell metabolism rate. Improves, reduces feedback inhibition, improves substrate conversion

Active Publication Date: 2014-05-21
SHANDONG FREDA BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The production method of pullulan polysaccharide is mainly biological fermentation method, but there have been many problems in its production process. Among them, the long period of Aureobasidium pullulans fermentation process, the secretion of a large amount of melanin and the low conversion rate of substrate sugar lead to the production Low, complicated separation and purification process, greatly increased production costs, reduced product quality, and became a key issue restricting the industrial production of pullulan. Therefore, there is currently no report on the industrial production of pullulan polysaccharide in China
[0003] In response to these problems, the methods commonly used at home and abroad are to obtain strains with less pigment production through screening and mutagenesis, but the strains obtained by these methods have low fermentation yields, the cycle is still too long, and there is still a small amount of pigment produced, which is difficult to remove

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Preparation and sterilization of seed medium: prepare fermentation medium according to the following formula

[0023] Sucrose 5%, yeast extract 0.2%, dipotassium hydrogen phosphate 0.5%, magnesium sulfate 0.02%, ammonium sulfate 0.06%, sodium chloride 0.1%. min;

[0024] (2) Seed preparation: isolate and culture the bacteria stored in the glycerol tube by streaking on the plate for 3 days, pick a single colony that has grown and put it into the seed bottle, and culture it on a shaking table at 28.5°C and 260 r / m for 30 hours;

[0025] (3) Preparation and sterilization of fermentation medium: prepare fermentation medium according to the following formula

[0026] Sucrose 10%, yeast extract 0.2%, dipotassium hydrogen phosphate 0.6%, magnesium sulfate 0.02%, ammonium sulfate 0.06%, sodium chloride 0.1%, polyoxypropylene ether 1%, Tween-20 0.05%, prepared and packaged After completion, use a high-pressure steam sterilizer to sterilize at 121°C for 20 minutes;

[0027...

Embodiment 2

[0030] (1) Preparation and sterilization of seed medium: prepare fermentation medium according to the following formula

[0031] 6% sucrose, 0.3% yeast extract, 0.6% dipotassium hydrogen phosphate, 0.03% magnesium sulfate, 0.05% ammonium sulfate, 0.05% sodium chloride. min;

[0032] (2) Seed preparation: isolate and culture the strains stored in the glycerol tube by streaking on the plate for 3 days, pick a single colony that has grown and put it into the seed bottle, and culture it on a shaking table at 28.5 °C and 260 r / m for 25 h;

[0033] (3) Preparation and sterilization of fermentation medium: prepare fermentation medium according to the following formula

[0034] Sucrose 12%, yeast extract 0.3%, dipotassium hydrogen phosphate 0.6%, magnesium sulfate 0.05%, ammonium sulfate 0.06%, sodium chloride 0.1%, polyoxyethylene polyoxypropylene ether 2%, Tween-80 0.1%, prepared , After the packaging is completed, use a high-pressure steam sterilizer to sterilize at 121°C for 20 ...

Embodiment 3

[0038] (1) Preparation and sterilization of seed medium: prepare fermentation medium according to the following formula

[0039] Sucrose 8%, yeast extract 0.5%, dipotassium hydrogen phosphate 0.5%, magnesium sulfate 0.02%, ammonium sulfate 0.06%, sodium chloride 0.05%. min;

[0040] (2) Seed preparation: isolate and culture the strains stored in the glycerol tube by streaking on a plate for 3 days, pick a single colony that has grown and put it into the seed bottle, and culture it on a shaking table at 28.5°C and 260 r / m for 24 hours;

[0041] (3) Preparation and sterilization of fermentation medium: prepare fermentation medium according to the following formula

[0042] Sucrose 15%, yeast extract 0.3%, dipotassium hydrogen phosphate 0.6%, magnesium sulfate 0.05%, ammonium sulfate 0.08%, sodium chloride 0.1%, polyvinyl ether 3%, Tween-80 0.1%, preparation and packaging After completion, use a high-pressure steam sterilizer to sterilize at 121°C for 20 minutes;

[0043] (4) ...

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PUM

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Abstract

The invention relates to a novel method for producing pullulan by using cell metabolism regulation technology to improve cell permeability. 0.02% to 2.14% of a polyether substance and 0.01% to 1.35% of a surfactant substance are added into a fermentation medium, thereby effectively increasing the yield of pullulan, improving the conversion efficiency of a substrate, shortening a fermentation cycle and reducing formation of melanin.

Description

technical field [0001] The invention relates to a pullulan production method based on a cell metabolism regulation strategy, belonging to the field of bioengineering. Background technique [0002] Pullulan is a water-soluble macromolecular exopolysaccharide produced by the fermentation of small molecule sugars such as sucrose and glucose by Aureobasidium pullulans. It is widely used due to its excellent film-forming, plasticity and gas barrier properties It is used in food preservation, medical materials and cosmetics. The production method of pullulan polysaccharide is mainly biological fermentation method, but there have been many problems in its production process. Among them, the long period of Aureobasidium pullulans fermentation process, the secretion of a large amount of melanin and the low conversion rate of substrate sugar lead to the production Low cost, complex separation and purification process, greatly increased production costs, reduced product quality, and b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C08B37/00C12R1/645
Inventor 朱云峰朱希强苏移山王林李伟凌沛学
Owner SHANDONG FREDA BIOTECH
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