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Application of soybean transcription factor GmMYB172 in vegetable oil and fat metabolism regulation

A technology of plant and oil content, applied in the biological field, can solve problems such as little known

Inactive Publication Date: 2014-09-24
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in plants, the regulatory mechanism of lipid synthesis and its related genes are still poorly understood

Method used

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  • Application of soybean transcription factor GmMYB172 in vegetable oil and fat metabolism regulation
  • Application of soybean transcription factor GmMYB172 in vegetable oil and fat metabolism regulation
  • Application of soybean transcription factor GmMYB172 in vegetable oil and fat metabolism regulation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1, cDNA cloning of the transcription factor GmMYB172 encoding gene related to the regulation of soybean and lipid metabolism and the construction of the plant expression vector

[0045] 1. Acquisition of transcription factor GmMYB172

[0046]A gene of the MYB transcription factor family, GmMYB172, was highly expressed during the analysis of fatty acid accumulation metabolism during soybean seed development.

[0047] Soybean Suinong 14 extracted (Hefeng 25 / Suinong 8, a new mid-maturing spring soybean variety bred by Suihua Agricultural Science Institute of Heilongjiang Academy of Agricultural Sciences, see China Agricultural Science and Technology Network, http: / / shucai.ag365.com / postdetail_351344.html. Provided by Manweiqun, Heilongjiang Academy of Agricultural Sciences. Recorded in the following documents: Qin Jun et al., Genetics, 28 (11), 1421-1427, 2006. Obtained) total RNA of seedlings, and the RNA was reverse-transcribed with reverse transcriptase to syn...

Embodiment 2

[0056] Embodiment 2, the acquisition of transgenic GmMYB172 Arabidopsis

[0057] 1. Obtaining recombinant Agrobacterium

[0058] The recombinant vector pPROKII-GmMYB172 obtained in Example 1 was introduced into Agrobacterium GV3101 by electroporation (Lee CW et al., Agrobacterium tumefaciens promotes tumor induction by modulating pathogen defense in Arabidopsis thaliana, Plant Cell, 2009, 21 (9), 2948-62; public available Obtained from the Institute of Genetics and Developmental Biology, Chinese Academy of Sciences), pick the recombinant Agrobacterium, extract the plasmid of the recombinant Agrobacterium and verify it by BamHI and KpnI double digestion, the insert fragment is a positive plasmid of about 222bp, and the recombination containing the positive plasmid Agrobacterium was named GV3101 / pPROKII-GmMYB172.

[0059] 2. Acquisition and identification of transgenic GmMYB172 Arabidopsis

[0060] The recombinant Agrobacterium GV3101 / pPROKII-GmMYB172 was cultivated to the log...

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Abstract

The invention discloses oil and fat metabolism regulation related soybean transcription factor GmMYB172 and a coding gene and application thereof. The protein is named as GmMYB172, is derived from papilionaceae glycine soybean (Glycine max (L.) Merrill), is a protein as follows (a) or (b): (a) protein is composed of an amino acid sequence shown as the sequence 2 in the sequence table; and (b) protein is a protein obtained by substitution and / or deletion and / or addition of one or a plurality of amino acid residues of the amino acid sequence shown as the sequence 2 and is in relevant to plant tissue total oil and fat content and derived from the sequence 2. Experiments show that, the transcription factor GmMYB172 and the coding gene can regulate the oil and fat content in plant seeds, and after over expression, can improve the oil and fat content in the plant seeds. The coding gene has important theoretical and practical significances in the aspects of increasing and improving crop oil and fat ingredients, especially improving oil and fat ingredients in soybean and other oil plant seeds, and cultivating high oil and fat containing varieties.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of soybean transcription factor GmMYB172 in the regulation and control of vegetable oil metabolism. Background technique [0002] 71% of the fat in the human diet comes from plants. Among several major oil-producing crops in the world, the total oil production of soybean accounts for about 30%, ranking first in the world's vegetable oil production (Table 1). [0003] Table 1 shows the main oil-producing crops in the world [0004] [0005] The synthesis of fatty acids is one of the most important metabolic pathways in plants. It exists in any cell of plants and is necessary for growth and development. Blocking it leads to cell death, so a plant mutant that blocks fatty acid synthesis has not been found so far. [0006] Plants differ greatly from other eukaryotes in the enzymes involved in the fatty acid synthesis pathway. Synthesis of fatty acids with 16 or 18 ...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N5/10C12N1/21C12N15/82C12N15/84A01H5/00
CPCC07K14/415C12N15/8247
Inventor 张劲松陈受宜刘云峰李擎天张万科马彪林晴何锶洁
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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