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Plant stress tolerance correlative protein kinase, encoding gene and application thereof

A technology that encodes genes and plant stress resistance. It is applied in the field of protein kinases related to plant stress resistance and its encoding genes and applications. It can solve the problems that the stress resistance regulatory genes have not yet been excavated.

Active Publication Date: 2010-07-14
BEIJING AGRO BIOTECH RES CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, previous studies were limited to morphology, anatomy, and physiology, and the stress-resistance regulatory genes that play a key role have not yet been excavated.

Method used

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  • Plant stress tolerance correlative protein kinase, encoding gene and application thereof
  • Plant stress tolerance correlative protein kinase, encoding gene and application thereof
  • Plant stress tolerance correlative protein kinase, encoding gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Example 1. Discovery and Analysis of Protein Kinases Related to Plant Stress Resistance and Their Encoding Genes

[0059] 1. Discovery of protein kinases and their encoding genes related to plant stress resistance

[0060] 1. Experimental steps

[0061] 1) Cultivation of barley grass (wild barley) material

[0062] Halophytic forage grass-hordeum brevisubulatum (Trin.) Link) is produced by the National Animal Husbandry and Veterinary Center for the Preservation and Utilization of Livestock and Poultry Grass Germplasm Resources. The seeds of barley grass (wild barley) were soaked in deionized water for 12 hours at room temperature, and then put into a refrigerator at 4° C. overnight. Rinse three times with sterilized deionized water, put them in a culture dish with wet gauze, and germinate at 25°C. After 4 to 5 days, when most of them germinate, transfer them to a sterilized glass bottle filled with 1 / 2 Hoagland culture solution (wrapped in opaque paper), at a tempera...

Embodiment 2

[0099] Embodiment 2, acquisition and detection of anti-reverse gene plants

[0100] 1. Acquisition of anti-retrograde genetic plants

[0101] 1. Acquisition of HbCIPK2 gene

[0102] Synthesize the following two primers:

[0103] HbCIPK2-BamHI-F: 5'-T GGATCC ATGGGGGAGCAGAAG-3';

[0104] HbCIPK2-SacI-R: 5'-TGAGCTCTCAACATGGTTGCTGCTG-3'.

[0105] Using the genomic DNA of halophytic pasture-Barley grass (wild barley) as a template, PCR amplification was carried out with the above two primers, and the amplified fragment HbCIPK2 was sequenced, and it was found that the amplified fragment had sequence 1 from 5 The nucleotides shown at positions 68-1426 of the ' end.

[0106] 2. Construction of recombinant expression vector

[0107] Insert the HbCIPK2 obtained in step 1 into pGreen0029 (containing the CaMV35 promoter and Nos terminator) (purchased from http: / / www.pgreen.ac.uk / ) between the BamHI and SacI restriction sites to form the expression vector pGreen0029 -HbCIPK2.

[0...

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Abstract

The invention discloses plant stress tolerance correlative protein kinase, an encoding gene and application thereof. The protein refers to the protein of 1) or 2): 1) the protein formed by an amino acid sequence shown by a second sequence in a sequence table; or 2) the plant stress tolerance correlative protein which is formed through the substitution and / or lack and / or addition of one or a plurality of amino acid residue radicals on the amino acid residue radical sequence of the second sequence in the sequence table and is derived from the protein of 1). The encoding gene of the protein is introduced into arabidopsis mutant sos2-1 or wild type arabidopsis for improving the salt tolerance and the drought tolerance of transgenic plants.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to protein kinases related to plant stress resistance, coding genes and applications thereof. Background technique [0002] Soil salinization, drought and other adversities are the main environmental limiting factors for plant growth and development. Plants cannot escape adversity, they can only cope. Therefore, plants have evolved a set of fine-tuning mechanisms for sensing adversity stress, transmitting adversity signals, and responding at the molecular, cellular, and physiological levels. Many studies have shown that various stresses act on plant cells, first triggering intracellular Ca 2+ change in concentration. Ca 2+ The change of concentration in time and space represents a specific stress information, which is called calcium signal. Calcium ion receptors can monitor calcium signals at any time, conduct signals through the proteins acting on them, and then regulate the expre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/12C12N15/54C12N15/63C12N15/82C12N1/15C12N1/19C12N1/21C12N5/10A01H5/00
Inventor 李瑞芬魏建华王宏芝
Owner BEIJING AGRO BIOTECH RES CENT
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